Restriction map for
pF1KB7841
(Circular) (Six-base) MAPSORT of: pF1KB7841.seq Check: 1422 from: 1 to: 4625
pF1KB7841 4625 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1663 1663
Size: 4625
Acc65I G'GTAC_C
Cuts at: 1641 1641
Size: 4625
AclI AA'CG_TT
Cuts at: 4465 4465
Size: 4625
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1282 2450 2882 3629 3897 1082
Size: 200 1168 432 747 268 1810
Fragments arranged by size:
1810 1168 747 432 268 200
AflIII A'CryG_T
Cuts at: 1977 3081 4058 1977
Size: 1104 977 2544
Fragments arranged by size:
2544 1104 977
AgeI A'CCGG_T
Cuts at: 1504 3013 1504
Size: 1509 3116
AleI CACnn'nnGTG
Cuts at: 704 704
Size: 4625
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1342 1345 1364 1342
Size: 3 19 4603
Fragments arranged by size:
4603 19 3
AlwNI CAG_nnn'CTG
Cuts at: 195 1930 3497 195
Size: 1735 1567 1323
Fragments arranged by size:
1735 1567 1323
ApaLI G'TGCA_C
Cuts at: 154 3395 154
Size: 3241 1384
ApoI r'AATT_y
Cuts at: 386 619 682 1088 1220 1241 1542 1629 3978
Size: 233 63 406 132 21 301 87 2349
Cuts at: 3978 4234 386
Size: 256 777
Fragments arranged by size:
2349 777 406 301 256 233 132 87
63 21
AseI AT'TA_AT
Cuts at: 20 1478 20
Size: 1458 3167
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
AvaI C'yCGr_G
Cuts at: 177 1645 177
Size: 1468 3157
BamHI G'GATC_C
Cuts at: 728 1650 728
Size: 922 3703
BanI G'GyrC_C
Cuts at: 199 1180 1380 1641 2055 2303 2338 199
Size: 981 200 261 414 248 35 2486
Fragments arranged by size:
2486 981 414 261 248 200 35
BanII G_rGCy'C
Cuts at: 1639 2669 1639
Size: 1030 3595
BbeI G_GCGC'C
Cuts at: 203 2059 2307 203
Size: 1856 248 2521
Fragments arranged by size:
2521 1856 248
BciVI GTATCCnnnnn_n'
Cuts at: 2523 3284 4625 2523
Size: 761 1341 2523
Fragments arranged by size:
2523 1341 761
BclI T'GATC_A
Cuts at: 1527 2145 1527
Size: 618 4007
BglI GCCn_nnn'nGGC
Cuts at: 179 179
Size: 4625
BglII A'GATC_T
Cuts at: 2140 4177 2140
Size: 2037 2588
BlpI GC'TnA_GC
Cuts at: 1737 1737
Size: 4625
Bme1580I G_kGCm'C
Cuts at: 158 722 1383 2250 2343 3399 158
Size: 564 661 867 93 1056 1384
Fragments arranged by size:
1384 1056 867 661 564 93
BmrI ACTGGGnnnn_n'
Cuts at: 205 1837 1918 2253 4397 205
Size: 1632 81 335 2144 433
Fragments arranged by size:
2144 1632 433 335 81
BmtI G_CTAG'C
Cuts at: 794 794
Size: 4625
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 890 2992 890
Size: 2102 2523
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 106 1801 2680 3172 3470 106
Size: 1695 879 492 298 1261
Fragments arranged by size:
1695 1261 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4329 4329
Size: 4625
BsaAI yAC'GTr
Cuts at: 1978 1978
Size: 4625
BsaBI GATnn'nnATC
Cuts at: 6 2139 4176 6
Size: 2133 2037 455
Fragments arranged by size:
2133 2037 455
BsaHI Gr'CG_yC
Cuts at: 167 200 2056 2304 3006 4502 167
Size: 33 1856 248 702 1496 290
Fragments arranged by size:
1856 1496 702 290 248 33
BsaWI w'CCGG_w
Cuts at: 1504 1814 2335 3013 3287 3434 3845 1504
Size: 310 521 678 274 147 411 2284
Fragments arranged by size:
2284 678 521 411 310 274 147
BseYI C'CCAG_C
Cuts at: 633 978 2050 2808 3385 3881 633
Size: 345 1072 758 577 496 1377
Fragments arranged by size:
1377 1072 758 577 496 345
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 845 1250 845
Size: 405 4220
BsiEI CG_ry'CG
Cuts at: 104 2167 2213 3421 104
Size: 2063 46 1208 1308
Fragments arranged by size:
2063 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 122 158 290 1012 1639 2417 2607 3399 122
Size: 36 132 722 627 778 190 792 1348
Fragments arranged by size:
1348 792 778 722 627 190 132 36
Bsp1286I G_dGCh'C
Cuts at: 122 158 290 722 1012 1383 1639 2250 2343
Size: 36 132 432 290 371 256 611 93
Cuts at: 2343 2417 2607 2669 3399 122
Size: 74 190 62 730 1348
Fragments arranged by size:
1348 730 611 432 371 290 256 190
132 93 74 62 36
BspEI T'CCGG_A
Cuts at: 3845 3845
Size: 4625
BspHI T'CATG_A
Cuts at: 876 3801 876
Size: 2925 1700
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1675 1889 2191 2572 1675
Size: 214 302 381 3728
Fragments arranged by size:
3728 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2917 2971 4455 4620 50
Size: 2867 54 1484 165 55
Fragments arranged by size:
2867 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 232 1129 2537 232
Size: 897 1408 2320
Fragments arranged by size:
2320 1408 897
BsrFI r'CCGG_y
Cuts at: 202 1504 2623 2804 3013 202
Size: 1302 1119 181 209 1814
Fragments arranged by size:
1814 1302 1119 209 181
BssSI C'ACGA_G
Cuts at: 2896 3254 2896
Size: 358 4267
BstAPI GCAn_nnn'nTGC
Cuts at: 1128 1357 2002 1128
Size: 229 645 3751
Fragments arranged by size:
3751 645 229
BstBI TT'CG_AA
Cuts at: 2986 2986
Size: 4625
BstYI r'GATC_y
Cuts at: 1 728 1650 2117 2140 2475 2721 3733 3819
Size: 727 922 467 23 335 246 1012 86
Cuts at: 3819 3831 4177 1
Size: 12 346 449
Fragments arranged by size:
1012 922 727 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 691 934 107
Size: 584 243 3798
Fragments arranged by size:
3798 584 243
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2575 2756 2919 2575
Size: 181 163 4281
Fragments arranged by size:
4281 181 163
BtsI GCAGTG_nn'
Cuts at: 666 1023 1362 1380 1868 666
Size: 357 339 18 488 3423
Fragments arranged by size:
3423 488 357 339 18
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 551 586 551
Size: 35 4590
DraI TTT'AAA
Cuts at: 1624 1624
Size: 4625
DraIII CAC_nnn'GTG
Cuts at: 926 926
Size: 4625
DrdI GACnn_nn'nnGTC
Cuts at: 2331 3189 2331
Size: 858 3767
EaeI y'GGCC_r
Cuts at: 2210 2384 2775 2802 4147 2210
Size: 174 391 27 1345 2688
Fragments arranged by size:
2688 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2210 2210
Size: 4625
EarI CTCTTCn'nnn_
Cuts at: 859 1152 2648 2858 859
Size: 293 1496 210 2626
Fragments arranged by size:
2626 1496 293 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2833 3143 3289 4435 2833
Size: 310 146 1146 3023
Fragments arranged by size:
3023 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1637 1637
Size: 4625
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1082 1282 2450 2882 3629 3897 1082
Size: 200 1168 432 747 268 1810
Fragments arranged by size:
1810 1168 747 432 268 200
EcoO109I rG'GnC_Cy
Cuts at: 630 1498 1764 630
Size: 868 266 3491
Fragments arranged by size:
3491 868 266
EcoRI G'AATT_C
Cuts at: 1241 1629 1241
Size: 388 4237
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2644 2644
Size: 4625
FspI TGC'GCA
Cuts at: 2406 2406
Size: 4625
HaeII r_GCGC'y
Cuts at: 203 209 985 2059 2307 3329 4308 203
Size: 6 776 1074 248 1022 979 520
Fragments arranged by size:
1074 1022 979 776 520 248 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2467 2497 2499 2529 2467
Size: 30 2 30 4563
Fragments arranged by size:
4563 30 30 2
HincII GTy'rAC
Cuts at: 738 1664 738
Size: 926 3699
KasI G'GCGC_C
Cuts at: 199 2055 2303 199
Size: 1856 248 2521
Fragments arranged by size:
2521 1856 248
KpnI G_GTAC'C
Cuts at: 1645 1645
Size: 4625
MluI A'CGCG_T
Cuts at: 4058 4058
Size: 4625
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3296 3480 3296
Size: 184 4441
MscI TGG'CCA
Cuts at: 2386 2386
Size: 4625
MslI CAynn'nnrTG
Cuts at: 704 1122 2614 2741 704
Size: 418 1492 127 2588
Fragments arranged by size:
2588 1492 418 127
MspA1I CmG'CkG
Cuts at: 1736 2050 2410 3423 3668 1736
Size: 314 360 1013 245 2693
Fragments arranged by size:
2693 1013 360 314 245
NaeI GCC'GGC
Cuts at: 204 2806 204
Size: 2602 2023
NarI GG'CG_CC
Cuts at: 200 2056 2304 200
Size: 1856 248 2521
Fragments arranged by size:
2521 1856 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4625
NdeI CA'TA_TG
Cuts at: 381 381
Size: 4625
NgoMIV G'CCGG_C
Cuts at: 202 2804 202
Size: 2602 2023
NheI G'CTAG_C
Cuts at: 790 790
Size: 4625
NspI r_CATG'y
Cuts at: 1678 2178 3085 1678
Size: 500 907 3218
Fragments arranged by size:
3218 907 500
PciI A'CATG_T
Cuts at: 3081 3081
Size: 4625
PflMI CCAn_nnn'nTGG
Cuts at: 113 274 113
Size: 161 4464
PfoI T'CCnGG_A
Cuts at: 1109 1109
Size: 4625
PmeI GTTT'AAAC
Cuts at: 1624 1624
Size: 4625
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3764 3796 3764
Size: 32 4593
PpuMI rG'GwC_Cy
Cuts at: 630 1498 630
Size: 868 3757
PstI C_TGCA'G
Cuts at: 1672 2357 1672
Size: 685 3940
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4625
PvuII CAG'CTG
Cuts at: 2050 2410 2050
Size: 360 4265
RsrII CG'GwC_CG
Cuts at: 2820 2820
Size: 4625
SacI G_AGCT'C
Cuts at: 1639 1639
Size: 4625
SalI G'TCGA_C
Cuts at: 1662 1662
Size: 4625
SanDI GG'GwC_CC
Cuts at: 630 1498 630
Size: 868 3757
SapI GCTCTTCn'nnn_
Cuts at: 2648 2858 2648
Size: 210 4415
SbfI CC_TGCA'GG
Cuts at: 1672 1672
Size: 4625
ScaI AGT'ACT
Cuts at: 4142 4142
Size: 4625
SfcI C'TryA_G
Cuts at: 33 1668 2353 3346 3537 33
Size: 1635 685 993 191 1121
Fragments arranged by size:
1635 1121 993 685 191
SfoI GGC'GCC
Cuts at: 201 2057 2305 201
Size: 1856 248 2521
Fragments arranged by size:
2521 1856 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4625
SgrAI Cr'CCGG_yG
Cuts at: 202 202
Size: 4625
SmaI CCC'GGG
Cuts at: 1647 1647
Size: 4625
SmlI C'TyrA_G
Cuts at: 121 1780 2695 3187 3449 121
Size: 1659 915 492 262 1297
Fragments arranged by size:
1659 1297 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4625
SphI G_CATG'C
Cuts at: 1678 2178 1678
Size: 500 4125
StyI C'CwwG_G
Cuts at: 107 369 1759 2113 107
Size: 262 1390 354 2619
Fragments arranged by size:
2619 1390 354 262
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 105 305 403 863 2208 2341 2800 3009 105
Size: 200 98 460 1345 133 459 209 1721
Fragments arranged by size:
1721 1345 460 459 209 200 133 98
TatI w'GTAC_w
Cuts at: 4140 4140
Size: 4625
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 399 431 4142 4174 399
Size: 32 3711 32 850
Fragments arranged by size:
3711 850 32 32
Tth111I GACn'n_nGTC
Cuts at: 1496 2422 1496
Size: 926 3699
XbaI T'CTAG_A
Cuts at: 1656 1656
Size: 4625
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 267 2120 267
Size: 1853 2772
XmaI C'CCGG_G
Cuts at: 1645 1645
Size: 4625
XmnI GAAnn'nnTTC
Cuts at: 1088 1088
Size: 4625
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstYI
BtgI BtgZI BtsI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BpmI
Bpu10I BsaXI BseRI BsiWI BsmI BsmBI BsrGI BssHII
BstEII BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI
FspAI HindIII HpaI MfeI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SexAI SfiI SnaBI SrfI SspI StuI SwaI XhoI
ZraI