Restriction map for
pF1KB7837
(Circular) (Six-base) MAPSORT of: pF1KB7837.seq Check: 3188 from: 1 to: 4733
pF1KB7837 4733 bp
With 182 enzymes: SgfI *
June 26, 2008 10:50 ..
AccI GT'mk_AC
Cuts at: 1771 1771
Size: 4733
Acc65I G'GTAC_C
Cuts at: 1749 1749
Size: 4733
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 537 957 1041 1461 1709 2558 2990 3737 4005
Size: 420 84 420 248 849 432 747 268
Cuts at: 4005 537
Size: 1265
Fragments arranged by size:
1265 849 747 432 420 420 268 248
84
AflIII A'CryG_T
Cuts at: 529 1673 2085 3189 4166 529
Size: 1144 412 1104 977 1096
Fragments arranged by size:
1144 1104 1096 977 412
AgeI A'CCGG_T
Cuts at: 3121 3121
Size: 4733
AleI CACnn'nnGTG
Cuts at: 1100 1520 1100
Size: 420 4313
AlwNI CAG_nnn'CTG
Cuts at: 381 2038 3605 381
Size: 1657 1567 1509
Fragments arranged by size:
1657 1567 1509
ApaLI G'TGCA_C
Cuts at: 3503 3503
Size: 4733
ApoI r'AATT_y
Cuts at: 353 923 1091 1737 4086 4342 353
Size: 570 168 646 2349 256 744
Fragments arranged by size:
2349 744 646 570 256 168
AseI AT'TA_AT
Cuts at: 20 20
Size: 4733
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 1753 1753
Size: 4733
BamHI G'GATC_C
Cuts at: 1758 1758
Size: 4733
BanI G'GyrC_C
Cuts at: 1749 2163 2411 2446 1749
Size: 414 248 35 4036
Fragments arranged by size:
4036 414 248 35
BanII G_rGCy'C
Cuts at: 1747 2777 1747
Size: 1030 3703
BbeI G_GCGC'C
Cuts at: 2167 2415 2167
Size: 248 4485
BbsI GAAGACnn'nnnn_
Cuts at: 666 834 1254 666
Size: 168 420 4145
Fragments arranged by size:
4145 420 168
BciVI GTATCCnnnnn_n'
Cuts at: 1243 2631 3392 4733 1243
Size: 1388 761 1341 1243
Fragments arranged by size:
1388 1341 1243 761
BclI T'GATC_A
Cuts at: 2253 2253
Size: 4733
BfrBI ATG'CAT
Cuts at: 1712 1712
Size: 4733
BglII A'GATC_T
Cuts at: 1144 1564 2248 4285 1144
Size: 420 684 2037 1592
Fragments arranged by size:
2037 1592 684 420
BlpI GC'TnA_GC
Cuts at: 1845 1845
Size: 4733
Bme1580I G_kGCm'C
Cuts at: 2358 2451 3507 2358
Size: 93 1056 3584
Fragments arranged by size:
3584 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 725 1945 2026 2361 4505 725
Size: 1220 81 335 2144 953
Fragments arranged by size:
2144 1220 953 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 424 622 3100 424
Size: 198 2478 2057
Fragments arranged by size:
2478 2057 198
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 617 785 869 953 1037 1205 1289 1373
Size: 198 168 84 84 84 168 84 84
Cuts at: 1373 1457 419
Size: 84 3695
Fragments arranged by size:
3695 198 168 168 84 84 84 84
84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1909 2788 3280 3578 1909
Size: 879 492 298 3064
Fragments arranged by size:
3064 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4437 4437
Size: 4733
BsaAI yAC'GTr
Cuts at: 532 2086 532
Size: 1554 3179
BsaBI GATnn'nnATC
Cuts at: 6 2247 4284 6
Size: 2241 2037 455
Fragments arranged by size:
2241 2037 455
BsaHI Gr'CG_yC
Cuts at: 2164 2412 3114 4610 2164
Size: 248 702 1496 2287
Fragments arranged by size:
2287 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1922 2443 3121 3395 3542 3953 1922
Size: 521 678 274 147 411 2702
Fragments arranged by size:
2702 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4733
BseYI C'CCAG_C
Cuts at: 2158 2916 3493 3989 2158
Size: 758 577 496 2902
Fragments arranged by size:
2902 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2275 2321 3529 104
Size: 2171 46 1208 1308
Fragments arranged by size:
2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1747 2525 2715 3507 1747
Size: 778 190 792 2973
Fragments arranged by size:
2973 792 778 190
BsmI GAATG_Cn'
Cuts at: 1039 1222 1039
Size: 183 4550
Bsp1286I G_dGCh'C
Cuts at: 1747 2358 2451 2525 2715 2777 3507 1747
Size: 611 93 74 190 62 730 2973
Fragments arranged by size:
2973 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3953 3953
Size: 4733
BspHI T'CATG_A
Cuts at: 3909 3909
Size: 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1783 1997 2299 2680 1783
Size: 214 302 381 3836
Fragments arranged by size:
3836 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3025 3079 4563 4728 50
Size: 2975 54 1484 165 55
Fragments arranged by size:
2975 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 964 1468 1620 2645 964
Size: 504 152 1025 3052
Fragments arranged by size:
3052 1025 504 152
BsrFI r'CCGG_y
Cuts at: 2731 2912 3121 2731
Size: 181 209 4343
Fragments arranged by size:
4343 209 181
BssSI C'ACGA_G
Cuts at: 3004 3362 3004
Size: 358 4375
BstAPI GCAn_nnn'nTGC
Cuts at: 2110 2110
Size: 4733
BstBI TT'CG_AA
Cuts at: 573 741 825 1329 1413 1497 3094 573
Size: 168 84 504 84 84 1597 2212
Fragments arranged by size:
2212 1597 504 168 84 84 84
BstYI r'GATC_y
Cuts at: 1 171 1144 1564 1758 2225 2248 2583 2829
Size: 170 973 420 194 467 23 335 246
Cuts at: 2829 3841 3927 3939 4285 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 973 467 449 420 346 335 246
194 170 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4733
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1560 2683 2864 3027 1560
Size: 1123 181 163 3266
Fragments arranged by size:
3266 1123 181 163
BtsI GCAGTG_nn'
Cuts at: 1048 1976 1048
Size: 928 3805
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1026 1061 1614 1649 1026
Size: 35 553 35 4110
Fragments arranged by size:
4110 553 35 35
DraI TTT'AAA
Cuts at: 948 1732 948
Size: 784 3949
DraIII CAC_nnn'GTG
Cuts at: 679 679
Size: 4733
DrdI GACnn_nn'nnGTC
Cuts at: 2439 3297 2439
Size: 858 3875
EaeI y'GGCC_r
Cuts at: 2318 2492 2883 2910 4255 2318
Size: 174 391 27 1345 2796
Fragments arranged by size:
2796 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 2756 2966 2756
Size: 210 4523
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2941 3251 3397 4543 2941
Size: 310 146 1146 3131
Fragments arranged by size:
3131 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1745 1745
Size: 4733
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 419 537 617 785 869 953 957 1037 1041
Size: 118 80 168 84 84 4 80 4
Cuts at: 1041 1205 1289 1373 1457 1461 1709 2558 2990
Size: 164 84 84 84 4 248 849 432
Cuts at: 2990 3737 4005 419
Size: 747 268 1147
Fragments arranged by size:
1147 849 747 432 268 248 168 164
118 84 84 84 84 84 80 80
4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1276 1872 1276
Size: 596 4137
EcoRI G'AATT_C
Cuts at: 923 1091 1737 923
Size: 168 646 3919
Fragments arranged by size:
3919 646 168
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2752 2752
Size: 4733
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 2167 2415 3437 4416 2167
Size: 248 1022 979 2484
Fragments arranged by size:
2484 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 653 685 2575 2605 2607 2637 653
Size: 32 1890 30 2 30 2749
Fragments arranged by size:
2749 1890 32 30 30 2
HincII GTy'rAC
Cuts at: 1679 1772 1679
Size: 93 4640
HindIII A'AGCT_T
Cuts at: 716 1388 716
Size: 672 4061
HpaI GTT'AAC
Cuts at: 1679 1679
Size: 4733
KasI G'GCGC_C
Cuts at: 2163 2411 2163
Size: 248 4485
KpnI G_GTAC'C
Cuts at: 1753 1753
Size: 4733
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 523 3404 3588 523
Size: 2881 184 1668
Fragments arranged by size:
2881 1668 184
MscI TGG'CCA
Cuts at: 2494 2494
Size: 4733
MslI CAynn'nnrTG
Cuts at: 696 1100 1520 2722 2849 696
Size: 404 420 1202 127 2580
Fragments arranged by size:
2580 1202 420 404 127
MspA1I CmG'CkG
Cuts at: 1844 2158 2518 3531 3776 1844
Size: 314 360 1013 245 2801
Fragments arranged by size:
2801 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2914 2914
Size: 4733
NarI GG'CG_CC
Cuts at: 2164 2412 2164
Size: 248 4485
NcoI C'CATG_G
Cuts at: 107 107
Size: 4733
NdeI CA'TA_TG
Cuts at: 305 305
Size: 4733
NgoMIV G'CCGG_C
Cuts at: 2912 2912
Size: 4733
NsiI A_TGCA'T
Cuts at: 1714 1714
Size: 4733
NspI r_CATG'y
Cuts at: 1677 1687 1716 1786 2286 3193 1677
Size: 10 29 70 500 907 3217
Fragments arranged by size:
3217 907 500 70 29 10
PciI A'CATG_T
Cuts at: 1673 3189 1673
Size: 1516 3217
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4701
PpuMI rG'GwC_Cy
Cuts at: 1276 1276
Size: 4733
PsiI TTA'TAA
Cuts at: 863 1283 1706 863
Size: 420 423 3890
Fragments arranged by size:
3890 423 420
PstI C_TGCA'G
Cuts at: 1780 2465 1780
Size: 685 4048
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 2158 2518 2158
Size: 360 4373
RsrII CG'GwC_CG
Cuts at: 2928 2928
Size: 4733
SacI G_AGCT'C
Cuts at: 1747 1747
Size: 4733
SalI G'TCGA_C
Cuts at: 1770 1770
Size: 4733
SapI GCTCTTCn'nnn_
Cuts at: 2756 2966 2756
Size: 210 4523
SbfI CC_TGCA'GG
Cuts at: 1780 1780
Size: 4733
ScaI AGT'ACT
Cuts at: 4250 4250
Size: 4733
SfcI C'TryA_G
Cuts at: 33 192 233 1776 2461 3454 3645 33
Size: 159 41 1543 685 993 191 1121
Fragments arranged by size:
1543 1121 993 685 191 159 41
SfoI GGC'GCC
Cuts at: 2165 2413 2165
Size: 248 4485
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SmaI CCC'GGG
Cuts at: 1755 1755
Size: 4733
SmlI C'TyrA_G
Cuts at: 1888 2803 3295 3557 1888
Size: 915 492 262 3064
Fragments arranged by size:
3064 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4733
SphI G_CATG'C
Cuts at: 1786 2286 1786
Size: 500 4233
StuI AGG'CCT
Cuts at: 1054 1054
Size: 4733
StyI C'CwwG_G
Cuts at: 107 1867 2221 107
Size: 1760 354 2619
Fragments arranged by size:
2619 1760 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 2316 2449 2908 3117 163
Size: 2153 133 459 209 1779
Fragments arranged by size:
2153 1779 459 209 133
TatI w'GTAC_w
Cuts at: 4248 4248
Size: 4733
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4250 4282 4250
Size: 32 4701
Tth111I GACn'n_nGTC
Cuts at: 2530 2530
Size: 4733
XbaI T'CTAG_A
Cuts at: 1764 1764
Size: 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 222 2228 114
Size: 108 2006 2619
Fragments arranged by size:
2619 2006 108
XmaI C'CCGG_G
Cuts at: 1753 1753
Size: 4733
XmnI GAAnn'nnTTC
Cuts at: 550 923 1054 1091 1111 1474 1511 550
Size: 373 131 37 20 363 37 3772
Fragments arranged by size:
3772 373 363 131 37 37 20
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII HpaI KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PsiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsaXI BsgI BsiWI BsmBI BsrGI BssHII BstEII
BstXI BstZ17I Bsu36I ClaI EcoNI EcoRV FseI FspAI
MfeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PshAI PspOMI PspXI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI SwaI XhoI ZraI