Restriction map for
pF1KB7835
(Circular) (Six-base) MAPSORT of: pF1KB7835.seq Check: 946 from: 1 to: 4733
pF1KB7835 4733 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1694 1694
Size: 4733
AccI GT'mk_AC
Cuts at: 206 1332 1771 206
Size: 1126 439 3168
Fragments arranged by size:
3168 1126 439
Acc65I G'GTAC_C
Cuts at: 453 1749 453
Size: 1296 3437
AclI AA'CG_TT
Cuts at: 4573 4573
Size: 4733
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2558 2990 3737 4005 2558
Size: 432 747 268 3286
Fragments arranged by size:
3286 747 432 268
AflIII A'CryG_T
Cuts at: 230 2085 3189 4166 230
Size: 1855 1104 977 797
Fragments arranged by size:
1855 1104 977 797
AgeI A'CCGG_T
Cuts at: 3121 3121
Size: 4733
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 362 362
Size: 4733
AlwNI CAG_nnn'CTG
Cuts at: 2038 3605 2038
Size: 1567 3166
ApaLI G'TGCA_C
Cuts at: 347 3503 347
Size: 3156 1577
ApoI r'AATT_y
Cuts at: 557 1054 1300 1737 4086 4342 557
Size: 497 246 437 2349 256 948
Fragments arranged by size:
2349 948 497 437 256 246
AseI AT'TA_AT
Cuts at: 20 850 20
Size: 830 3903
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
AvaI C'yCGr_G
Cuts at: 1753 1753
Size: 4733
AvrII C'CTAG_G
Cuts at: 514 514
Size: 4733
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 437 470 437
Size: 33 4700
BamHI G'GATC_C
Cuts at: 1109 1758 1109
Size: 649 4084
BanI G'GyrC_C
Cuts at: 453 1551 1749 2163 2411 2446 453
Size: 1098 198 414 248 35 2740
Fragments arranged by size:
2740 1098 414 248 198 35
BanII G_rGCy'C
Cuts at: 876 1747 2777 876
Size: 871 1030 2832
Fragments arranged by size:
2832 1030 871
BbeI G_GCGC'C
Cuts at: 2167 2415 2167
Size: 248 4485
BbsI GAAGACnn'nnnn_
Cuts at: 239 1436 239
Size: 1197 3536
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 634 668 634
Size: 34 4699
BciVI GTATCCnnnnn_n'
Cuts at: 2631 3392 4733 2631
Size: 761 1341 2631
Fragments arranged by size:
2631 1341 761
BclI T'GATC_A
Cuts at: 2253 2253
Size: 4733
BglII A'GATC_T
Cuts at: 2248 4285 2248
Size: 2037 2696
BlpI GC'TnA_GC
Cuts at: 1715 1845 1715
Size: 130 4603
Bme1580I G_kGCm'C
Cuts at: 351 1554 2358 2451 3507 351
Size: 1203 804 93 1056 1577
Fragments arranged by size:
1577 1203 1056 804 93
BmgBI CAC'GTC
Cuts at: 435 435
Size: 4733
BmrI ACTGGGnnnn_n'
Cuts at: 1945 2026 2361 4505 1945
Size: 81 335 2144 2173
Fragments arranged by size:
2173 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3100 3100
Size: 4733
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1082 1647 948
Size: 134 565 4034
Fragments arranged by size:
4034 565 134
Bpu10I CC'TnA_GC
Cuts at: 1640 1640
Size: 4733
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1133 1322 1909 2788 3280 3578 1133
Size: 189 587 879 492 298 2288
Fragments arranged by size:
2288 879 587 492 298 189
BsaI GGTCTCn'nnnn_
Cuts at: 4437 4437
Size: 4733
BsaAI yAC'GTr
Cuts at: 2086 2086
Size: 4733
BsaBI GATnn'nnATC
Cuts at: 6 2247 4284 6
Size: 2241 2037 455
Fragments arranged by size:
2241 2037 455
BsaHI Gr'CG_yC
Cuts at: 2164 2412 3114 4610 2164
Size: 248 702 1496 2287
Fragments arranged by size:
2287 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1922 2443 3121 3395 3542 3953 1922
Size: 521 678 274 147 411 2702
Fragments arranged by size:
2702 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1454 1484 1454
Size: 30 4703
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1478 1478
Size: 4733
BseYI C'CCAG_C
Cuts at: 798 2158 2916 3493 3989 798
Size: 1360 758 577 496 1542
Fragments arranged by size:
1542 1360 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2275 2321 3529 104
Size: 2171 46 1208 1308
Fragments arranged by size:
2171 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 351 876 1747 2525 2715 3507 351
Size: 525 871 778 190 792 1577
Fragments arranged by size:
1577 871 792 778 525 190
BsmBI CGTCTCn'nnnn_
Cuts at: 1460 1460
Size: 4733
Bsp1286I G_dGCh'C
Cuts at: 351 876 1554 1747 2358 2451 2525 2715 2777
Size: 525 678 193 611 93 74 190 62
Cuts at: 2777 3507 351
Size: 730 1577
Fragments arranged by size:
1577 730 678 611 525 193 190 93
74 62
BspEI T'CCGG_A
Cuts at: 3953 3953
Size: 4733
BspHI T'CATG_A
Cuts at: 3909 3909
Size: 4733
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1694 1783 1997 2299 2680 1694
Size: 89 214 302 381 3747
Fragments arranged by size:
3747 381 302 214 89
BsrBI CCG'CTC
Cuts at: 50 3025 3079 4563 4728 50
Size: 2975 54 1484 165 55
Fragments arranged by size:
2975 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2645 2645
Size: 4733
BsrFI r'CCGG_y
Cuts at: 2731 2912 3121 2731
Size: 181 209 4343
Fragments arranged by size:
4343 209 181
BssSI C'ACGA_G
Cuts at: 3004 3362 3004
Size: 358 4375
BstAPI GCAn_nnn'nTGC
Cuts at: 2110 2110
Size: 4733
BstBI TT'CG_AA
Cuts at: 3094 3094
Size: 4733
BstEII G'GTnAC_C
Cuts at: 835 835
Size: 4733
BstYI r'GATC_y
Cuts at: 1 975 1109 1758 2225 2248 2583 2829 3841
Size: 974 134 649 467 23 335 246 1012
Cuts at: 3841 3927 3939 4285 1
Size: 86 12 346 449
Fragments arranged by size:
1012 974 649 467 449 346 335 246
134 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4733
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2683 2864 3027 2683
Size: 181 163 4389
Fragments arranged by size:
4389 181 163
BtsI GCAGTG_nn'
Cuts at: 1496 1976 1496
Size: 480 4253
DraI TTT'AAA
Cuts at: 1732 1732
Size: 4733
DrdI GACnn_nn'nnGTC
Cuts at: 2439 3297 2439
Size: 858 3875
EaeI y'GGCC_r
Cuts at: 1416 2318 2492 2883 2910 4255 1416
Size: 902 174 391 27 1345 1894
Fragments arranged by size:
1894 1345 902 391 174 27
EagI C'GGCC_G
Cuts at: 2318 2318
Size: 4733
EarI CTCTTCn'nnn_
Cuts at: 864 1444 2756 2966 864
Size: 580 1312 210 2631
Fragments arranged by size:
2631 1312 580 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2941 3251 3397 4543 2941
Size: 310 146 1146 3131
Fragments arranged by size:
3131 1146 310 146
EcoICRI GAG'CTC
Cuts at: 874 1745 874
Size: 871 3862
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 948 1082 1647 2558 2990 3737 4005 948
Size: 134 565 911 432 747 268 1676
Fragments arranged by size:
1676 911 747 565 432 268 134
EcoNI CCTnn'n_nnAGG
Cuts at: 1590 1590
Size: 4733
EcoO109I rG'GnC_Cy
Cuts at: 1872 1872
Size: 4733
EcoRI G'AATT_C
Cuts at: 557 1737 557
Size: 1180 3553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 893 2752 893
Size: 1859 2874
FspI TGC'GCA
Cuts at: 2514 2514
Size: 4733
HaeII r_GCGC'y
Cuts at: 2167 2415 3437 4416 2167
Size: 248 1022 979 2484
Fragments arranged by size:
2484 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 967 988 999 1020 2575 2605 2607 2637 967
Size: 21 11 21 1555 30 2 30 3063
Fragments arranged by size:
3063 1555 30 30 21 21 11 2
HincII GTy'rAC
Cuts at: 207 1190 1333 1772 207
Size: 983 143 439 3168
Fragments arranged by size:
3168 983 439 143
HpaI GTT'AAC
Cuts at: 1190 1190
Size: 4733
KasI G'GCGC_C
Cuts at: 2163 2411 2163
Size: 248 4485
KpnI G_GTAC'C
Cuts at: 457 1753 457
Size: 1296 3437
MluI A'CGCG_T
Cuts at: 4166 4166
Size: 4733
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 864 1003 3404 3588 864
Size: 139 2401 184 2009
Fragments arranged by size:
2401 2009 184 139
MscI TGG'CCA
Cuts at: 2494 2494
Size: 4733
MslI CAynn'nnrTG
Cuts at: 2722 2849 2722
Size: 127 4606
MspA1I CmG'CkG
Cuts at: 309 1844 2158 2518 3531 3776 309
Size: 1535 314 360 1013 245 1266
Fragments arranged by size:
1535 1266 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2914 2914
Size: 4733
NarI GG'CG_CC
Cuts at: 2164 2412 2164
Size: 248 4485
NcoI C'CATG_G
Cuts at: 107 107
Size: 4733
NgoMIV G'CCGG_C
Cuts at: 2912 2912
Size: 4733
NspI r_CATG'y
Cuts at: 171 234 1786 2286 3193 171
Size: 63 1552 500 907 1711
Fragments arranged by size:
1711 1552 907 500 63
PasI CC'CwG_GG
Cuts at: 1313 1313
Size: 4733
PciI A'CATG_T
Cuts at: 230 3189 230
Size: 2959 1774
PmeI GTTT'AAAC
Cuts at: 1732 1732
Size: 4733
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3872 3904 3872
Size: 32 4701
PstI C_TGCA'G
Cuts at: 1491 1780 2465 1491
Size: 289 685 3759
Fragments arranged by size:
3759 685 289
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4733
PvuII CAG'CTG
Cuts at: 309 2158 2518 309
Size: 1849 360 2524
Fragments arranged by size:
2524 1849 360
RsrII CG'GwC_CG
Cuts at: 2928 2928
Size: 4733
SacI G_AGCT'C
Cuts at: 876 1747 876
Size: 871 3862
SalI G'TCGA_C
Cuts at: 205 1331 1770 205
Size: 1126 439 3168
Fragments arranged by size:
3168 1126 439
SapI GCTCTTCn'nnn_
Cuts at: 864 2756 2966 864
Size: 1892 210 2631
Fragments arranged by size:
2631 1892 210
SbfI CC_TGCA'GG
Cuts at: 1780 1780
Size: 4733
ScaI AGT'ACT
Cuts at: 1369 4250 1369
Size: 2881 1852
SexAI A'CCwGG_T
Cuts at: 152 152
Size: 4733
SfcI C'TryA_G
Cuts at: 33 237 1487 1776 2461 3454 3645 33
Size: 204 1250 289 685 993 191 1121
Fragments arranged by size:
1250 1121 993 685 289 204 191
SfoI GGC'GCC
Cuts at: 2165 2413 2165
Size: 248 4485
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4733
SmaI CCC'GGG
Cuts at: 1755 1755
Size: 4733
SmlI C'TyrA_G
Cuts at: 1148 1337 1888 2803 3295 3557 1148
Size: 189 551 915 492 262 2324
Fragments arranged by size:
2324 915 551 492 262 189
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4733
SphI G_CATG'C
Cuts at: 1786 2286 1786
Size: 500 4233
StuI AGG'CCT
Cuts at: 356 1265 356
Size: 909 3824
StyI C'CwwG_G
Cuts at: 107 514 860 1867 2221 107
Size: 407 346 1007 354 2619
Fragments arranged by size:
2619 1007 407 354 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 254 827 2316 2449 2908 3117 254
Size: 573 1489 133 459 209 1870
Fragments arranged by size:
1870 1489 573 459 209 133
TatI w'GTAC_w
Cuts at: 233 1039 1367 4248 233
Size: 806 328 2881 718
Fragments arranged by size:
2881 806 718 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1675 1707 4250 4282 1675
Size: 32 2543 32 2126
Fragments arranged by size:
2543 2126 32 32
Tth111I GACn'n_nGTC
Cuts at: 2530 2530
Size: 4733
XbaI T'CTAG_A
Cuts at: 1764 1764
Size: 4733
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 538 2228 538
Size: 1690 3043
XmaI C'CCGG_G
Cuts at: 1753 1753
Size: 4733
XmnI GAAnn'nnTTC
Cuts at: 944 1433 944
Size: 489 4244
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HpaI KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PasI PciI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI ApaI AscI
BbvCI BfrBI BglI BmtI BsgI BsiWI BsmI BsrGI
BssHII BstXI BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII MfeI NdeI NheI NotI NruI
NsiI PacI PflMI PfoI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI