Restriction map for
pF1KB7833
(Circular) (Six-base) MAPSORT of: pF1KB7833.seq Check: 8122 from: 1 to: 4727
pF1KB7833 4727 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1765 1765
Size: 4727
Acc65I G'GTAC_C
Cuts at: 1743 1743
Size: 4727
AclI AA'CG_TT
Cuts at: 4567 4567
Size: 4727
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 474 558 1512 2552 2984 3731 3999 474
Size: 84 954 1040 432 747 268 1202
Fragments arranged by size:
1202 1040 954 747 432 268 84
AflII C'TTAA_G
Cuts at: 424 424
Size: 4727
AflIII A'CryG_T
Cuts at: 2079 3183 4160 2079
Size: 1104 977 2646
Fragments arranged by size:
2646 1104 977
AgeI A'CCGG_T
Cuts at: 3115 3115
Size: 4727
AleI CACnn'nnGTG
Cuts at: 1235 1571 1235
Size: 336 4391
AlwNI CAG_nnn'CTG
Cuts at: 674 2032 3599 674
Size: 1358 1567 1802
Fragments arranged by size:
1802 1567 1358
ApaLI G'TGCA_C
Cuts at: 3497 3497
Size: 4727
ApoI r'AATT_y
Cuts at: 312 722 823 925 1021 1731 4080 4336 312
Size: 410 101 102 96 710 2349 256 703
Fragments arranged by size:
2349 710 703 410 256 102 101 96
AseI AT'TA_AT
Cuts at: 20 284 1280 20
Size: 264 996 3467
Fragments arranged by size:
3467 996 264
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
AvaI C'yCGr_G
Cuts at: 1747 1747
Size: 4727
BamHI G'GATC_C
Cuts at: 1752 1752
Size: 4727
BanI G'GyrC_C
Cuts at: 1743 2157 2405 2440 1743
Size: 414 248 35 4030
Fragments arranged by size:
4030 414 248 35
BanII G_rGCy'C
Cuts at: 1741 2771 1741
Size: 1030 3697
BbeI G_GCGC'C
Cuts at: 2161 2409 2161
Size: 248 4479
BbvCI CC'TCA_GC
Cuts at: 305 305
Size: 4727
BciVI GTATCCnnnnn_n'
Cuts at: 2625 3386 4727 2625
Size: 761 1341 2625
Fragments arranged by size:
2625 1341 761
BclI T'GATC_A
Cuts at: 2247 2247
Size: 4727
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4727
BglII A'GATC_T
Cuts at: 954 2242 4279 954
Size: 1288 2037 1402
Fragments arranged by size:
2037 1402 1288
BlpI GC'TnA_GC
Cuts at: 1839 1839
Size: 4727
Bme1580I G_kGCm'C
Cuts at: 2352 2445 3501 2352
Size: 93 1056 3578
Fragments arranged by size:
3578 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1939 2020 2355 4499 1939
Size: 81 335 2144 2167
Fragments arranged by size:
2167 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3094 3094
Size: 4727
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 1088 1172 259
Size: 829 84 3814
Fragments arranged by size:
3814 829 84
Bpu10I CC'TnA_GC
Cuts at: 305 305
Size: 4727
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 426 632 794 1903 2782 3274 3572 426
Size: 206 162 1109 879 492 298 1581
Fragments arranged by size:
1581 1109 879 492 298 206 162
BsaI GGTCTCn'nnnn_
Cuts at: 732 4431 732
Size: 3699 1028
BsaAI yAC'GTr
Cuts at: 2080 2080
Size: 4727
BsaBI GATnn'nnATC
Cuts at: 6 2241 4278 6
Size: 2235 2037 455
Fragments arranged by size:
2235 2037 455
BsaHI Gr'CG_yC
Cuts at: 2158 2406 3108 4604 2158
Size: 248 702 1496 2281
Fragments arranged by size:
2281 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1916 2437 3115 3389 3536 3947 1916
Size: 521 678 274 147 411 2696
Fragments arranged by size:
2696 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 687 1443 199
Size: 488 756 3483
Fragments arranged by size:
3483 756 488
BseYI C'CCAG_C
Cuts at: 115 2152 2910 3487 3983 115
Size: 2037 758 577 496 859
Fragments arranged by size:
2037 859 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1424 1424
Size: 4727
BsiEI CG_ry'CG
Cuts at: 104 2269 2315 3523 104
Size: 2165 46 1208 1308
Fragments arranged by size:
2165 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1741 2519 2709 3501 1741
Size: 778 190 792 2967
Fragments arranged by size:
2967 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1741 2352 2445 2519 2709 2771 3501 1741
Size: 611 93 74 190 62 730 2967
Fragments arranged by size:
2967 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3947 3947
Size: 4727
BspHI T'CATG_A
Cuts at: 3903 3903
Size: 4727
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1777 1991 2293 2674 1777
Size: 214 302 381 3830
Fragments arranged by size:
3830 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3019 3073 4557 4722 50
Size: 2969 54 1484 165 55
Fragments arranged by size:
2969 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2639 2639
Size: 4727
BsrFI r'CCGG_y
Cuts at: 2725 2906 3115 2725
Size: 181 209 4337
Fragments arranged by size:
4337 209 181
BsrGI T'GTAC_A
Cuts at: 795 1215 795
Size: 420 4307
BssSI C'ACGA_G
Cuts at: 2998 3356 2998
Size: 358 4369
BstAPI GCAn_nnn'nTGC
Cuts at: 2104 2104
Size: 4727
BstBI TT'CG_AA
Cuts at: 3088 3088
Size: 4727
BstXI CCAn_nnnn'nTGG
Cuts at: 342 342
Size: 4727
BstYI r'GATC_y
Cuts at: 1 954 1752 2219 2242 2577 2823 3835 3921
Size: 953 798 467 23 335 246 1012 86
Cuts at: 3921 3933 4279 1
Size: 12 346 449
Fragments arranged by size:
1012 953 798 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4727
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2677 2858 3021 2677
Size: 181 163 4383
Fragments arranged by size:
4383 181 163
BtsI GCAGTG_nn'
Cuts at: 675 1970 675
Size: 1295 3432
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1077 1112 1077
Size: 35 4692
DraI TTT'AAA
Cuts at: 1296 1726 1296
Size: 430 4297
DrdI GACnn_nn'nnGTC
Cuts at: 2433 3291 2433
Size: 858 3869
EaeI y'GGCC_r
Cuts at: 2312 2486 2877 2904 4249 2312
Size: 174 391 27 1345 2790
Fragments arranged by size:
2790 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2312 2312
Size: 4727
EarI CTCTTCn'nnn_
Cuts at: 1206 1293 2750 2960 1206
Size: 87 1457 210 2973
Fragments arranged by size:
2973 1457 210 87
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2935 3245 3391 4537 2935
Size: 310 146 1146 3125
Fragments arranged by size:
3125 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1739 1739
Size: 4727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 474 558 1088 1172 1512 2552 2984 3731
Size: 215 84 530 84 340 1040 432 747
Cuts at: 3731 3999 259
Size: 268 987
Fragments arranged by size:
1040 987 747 530 432 340 268 215
84 84
EcoO109I rG'GnC_Cy
Cuts at: 1866 1866
Size: 4727
EcoRI G'AATT_C
Cuts at: 1731 1731
Size: 4727
EcoRV GAT'ATC
Cuts at: 531 531
Size: 4727
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2746 2746
Size: 4727
FspI TGC'GCA
Cuts at: 2508 2508
Size: 4727
HaeII r_GCGC'y
Cuts at: 2161 2409 3431 4410 2161
Size: 248 1022 979 2478
Fragments arranged by size:
2478 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2569 2599 2601 2631 2569
Size: 30 2 30 4665
Fragments arranged by size:
4665 30 30 2
HincII GTy'rAC
Cuts at: 125 1342 1468 1766 125
Size: 1217 126 298 3086
Fragments arranged by size:
3086 1217 298 126
HindIII A'AGCT_T
Cuts at: 1187 1187
Size: 4727
KasI G'GCGC_C
Cuts at: 2157 2405 2157
Size: 248 4479
KpnI G_GTAC'C
Cuts at: 1747 1747
Size: 4727
MluI A'CGCG_T
Cuts at: 4160 4160
Size: 4727
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3398 3582 3398
Size: 184 4543
MscI TGG'CCA
Cuts at: 2488 2488
Size: 4727
MslI CAynn'nnrTG
Cuts at: 663 1235 1319 1503 1571 2716 2843 663
Size: 572 84 184 68 1145 127 2547
Fragments arranged by size:
2547 1145 572 184 127 84 68
MspA1I CmG'CkG
Cuts at: 1838 2152 2512 3525 3770 1838
Size: 314 360 1013 245 2795
Fragments arranged by size:
2795 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2908 2908
Size: 4727
NarI GG'CG_CC
Cuts at: 2158 2406 2158
Size: 248 4479
NcoI C'CATG_G
Cuts at: 107 107
Size: 4727
NdeI CA'TA_TG
Cuts at: 1220 1220
Size: 4727
NgoMIV G'CCGG_C
Cuts at: 2906 2906
Size: 4727
NspI r_CATG'y
Cuts at: 804 845 1308 1780 2280 3187 804
Size: 41 463 472 500 907 2344
Fragments arranged by size:
2344 907 500 472 463 41
PciI A'CATG_T
Cuts at: 3183 3183
Size: 4727
PmeI GTTT'AAAC
Cuts at: 1726 1726
Size: 4727
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3866 3898 3866
Size: 32 4695
PsiI TTA'TAA
Cuts at: 1129 1129
Size: 4727
PstI C_TGCA'G
Cuts at: 1774 2459 1774
Size: 685 4042
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4727
PvuII CAG'CTG
Cuts at: 2152 2512 2152
Size: 360 4367
RsrII CG'GwC_CG
Cuts at: 2922 2922
Size: 4727
SacI G_AGCT'C
Cuts at: 1741 1741
Size: 4727
SalI G'TCGA_C
Cuts at: 1764 1764
Size: 4727
SapI GCTCTTCn'nnn_
Cuts at: 2750 2960 2750
Size: 210 4517
SbfI CC_TGCA'GG
Cuts at: 1774 1774
Size: 4727
ScaI AGT'ACT
Cuts at: 4244 4244
Size: 4727
SfcI C'TryA_G
Cuts at: 33 782 911 1770 2455 3448 3639 33
Size: 749 129 859 685 993 191 1121
Fragments arranged by size:
1121 993 859 749 685 191 129
SfoI GGC'GCC
Cuts at: 2159 2407 2159
Size: 248 4479
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4727
SmaI CCC'GGG
Cuts at: 1749 1749
Size: 4727
SmlI C'TyrA_G
Cuts at: 405 424 647 809 1882 2797 3289 3551 405
Size: 19 223 162 1073 915 492 262 1581
Fragments arranged by size:
1581 1073 915 492 262 223 162 19
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4727
SphI G_CATG'C
Cuts at: 1780 2280 1780
Size: 500 4227
StuI AGG'CCT
Cuts at: 1413 1544 1413
Size: 131 4596
StyI C'CwwG_G
Cuts at: 107 1861 2215 107
Size: 1754 354 2619
Fragments arranged by size:
2619 1754 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 107 193 1607 2310 2443 2902 3111 107
Size: 86 1414 703 133 459 209 1723
Fragments arranged by size:
1723 1414 703 459 209 133 86
TatI w'GTAC_w
Cuts at: 795 1215 1551 4242 795
Size: 420 336 2691 1280
Fragments arranged by size:
2691 1280 420 336
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4244 4276 4244
Size: 32 4695
Tth111I GACn'n_nGTC
Cuts at: 2524 2524
Size: 4727
XbaI T'CTAG_A
Cuts at: 1758 1758
Size: 4727
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2222 2222
Size: 4727
XmaI C'CCGG_G
Cuts at: 1747 1747
Size: 4727
XmnI GAAnn'nnTTC
Cuts at: 853 1105 1357 853
Size: 252 252 4223
Fragments arranged by size:
4223 252 252
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NspI PciI
PmeI PpiI PsiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsmBI BssHII BstEII BstZ17I Bsu36I ClaI
DraIII EcoNI FseI FspAI HpaI MfeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmlI PpuMI
PshAI PspOMI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI ZraI