Restriction map for
pF1KB7832
(Circular) (Six-base) MAPSORT of: pF1KB7832.seq Check: 3974 from: 1 to: 4943
pF1KB7832 4943 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AccI GT'mk_AC
Cuts at: 1981 1981
Size: 4943
Acc65I G'GTAC_C
Cuts at: 1959 1959
Size: 4943
AclI AA'CG_TT
Cuts at: 4783 4783
Size: 4943
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2768 3200 3947 4215 2768
Size: 432 747 268 3496
Fragments arranged by size:
3496 747 432 268
AflIII A'CryG_T
Cuts at: 1024 2295 3399 4376 1024
Size: 1271 1104 977 1591
Fragments arranged by size:
1591 1271 1104 977
AgeI A'CCGG_T
Cuts at: 3331 3331
Size: 4943
AleI CACnn'nnGTG
Cuts at: 125 1109 125
Size: 984 3959
AlwNI CAG_nnn'CTG
Cuts at: 333 2248 3815 333
Size: 1915 1567 1461
Fragments arranged by size:
1915 1567 1461
ApaLI G'TGCA_C
Cuts at: 859 3713 859
Size: 2854 2089
ApoI r'AATT_y
Cuts at: 202 389 1623 1644 1947 4296 4552 202
Size: 187 1234 21 303 2349 256 593
Fragments arranged by size:
2349 1234 593 303 256 187 21
AseI AT'TA_AT
Cuts at: 20 20
Size: 4943
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4943
AvaI C'yCGr_G
Cuts at: 811 1963 811
Size: 1152 3791
BamHI G'GATC_C
Cuts at: 1968 1968
Size: 4943
BanI G'GyrC_C
Cuts at: 1959 2373 2621 2656 1959
Size: 414 248 35 4246
Fragments arranged by size:
4246 414 248 35
BanII G_rGCy'C
Cuts at: 1565 1957 2987 1565
Size: 392 1030 3521
Fragments arranged by size:
3521 1030 392
BbeI G_GCGC'C
Cuts at: 2377 2625 2377
Size: 248 4695
BbsI GAAGACnn'nnnn_
Cuts at: 529 1221 529
Size: 692 4251
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4909
BciVI GTATCCnnnnn_n'
Cuts at: 1842 2841 3602 4943 1842
Size: 999 761 1341 1842
Fragments arranged by size:
1842 1341 999 761
BclI T'GATC_A
Cuts at: 297 2463 297
Size: 2166 2777
BfrBI ATG'CAT
Cuts at: 727 1245 727
Size: 518 4425
BglII A'GATC_T
Cuts at: 2458 4495 2458
Size: 2037 2906
BlpI GC'TnA_GC
Cuts at: 2055 2055
Size: 4943
Bme1580I G_kGCm'C
Cuts at: 863 2568 2661 3717 863
Size: 1705 93 1056 2089
Fragments arranged by size:
2089 1705 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 701 2155 2236 2571 4715 701
Size: 1454 81 335 2144 929
Fragments arranged by size:
2144 1454 929 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1397 3310 1397
Size: 1913 3030
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1683 1442
Size: 241 4702
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1297 1367 2119 2998 3490 3788 1297
Size: 70 752 879 492 298 2452
Fragments arranged by size:
2452 879 752 492 298 70
BsaI GGTCTCn'nnnn_
Cuts at: 4647 4647
Size: 4943
BsaAI yAC'GTr
Cuts at: 2296 2296
Size: 4943
BsaBI GATnn'nnATC
Cuts at: 6 2457 4494 6
Size: 2451 2037 455
Fragments arranged by size:
2451 2037 455
BsaHI Gr'CG_yC
Cuts at: 2374 2622 3324 4820 2374
Size: 248 702 1496 2497
Fragments arranged by size:
2497 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2132 2653 3331 3605 3752 4163 2132
Size: 521 678 274 147 411 2912
Fragments arranged by size:
2912 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1842 1872 1842
Size: 30 4913
BseYI C'CCAG_C
Cuts at: 2368 3126 3703 4199 2368
Size: 758 577 496 3112
Fragments arranged by size:
3112 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1700 1724 1700
Size: 24 4919
BsiEI CG_ry'CG
Cuts at: 104 2485 2531 3739 104
Size: 2381 46 1208 1308
Fragments arranged by size:
2381 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 134 863 912 1565 1957 2735 2925 3717 134
Size: 729 49 653 392 778 190 792 1360
Fragments arranged by size:
1360 792 778 729 653 392 190 49
BsmI GAATG_Cn'
Cuts at: 729 729
Size: 4943
Bsp1286I G_dGCh'C
Cuts at: 134 863 912 1565 1957 2568 2661 2735 2925
Size: 729 49 653 392 611 93 74 190
Cuts at: 2925 2987 3717 134
Size: 62 730 1360
Fragments arranged by size:
1360 730 729 653 611 392 190 93
74 62 49
BspEI T'CCGG_A
Cuts at: 4163 4163
Size: 4943
BspHI T'CATG_A
Cuts at: 4119 4119
Size: 4943
BspMI ACCTGCnnnn'nnnn_
Cuts at: 157 1993 2207 2509 2890 157
Size: 1836 214 302 381 2210
Fragments arranged by size:
2210 1836 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3235 3289 4773 4938 50
Size: 3185 54 1484 165 55
Fragments arranged by size:
3185 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2855 2855
Size: 4943
BsrFI r'CCGG_y
Cuts at: 2941 3122 3331 2941
Size: 181 209 4553
Fragments arranged by size:
4553 209 181
BssSI C'ACGA_G
Cuts at: 3214 3572 3214
Size: 358 4585
BstAPI GCAn_nnn'nTGC
Cuts at: 2320 2320
Size: 4943
BstBI TT'CG_AA
Cuts at: 3304 3304
Size: 4943
BstXI CCAn_nnnn'nTGG
Cuts at: 1111 1111
Size: 4943
BstYI r'GATC_y
Cuts at: 1 1968 2435 2458 2793 3039 4051 4137 4149
Size: 1967 467 23 335 246 1012 86 12
Cuts at: 4149 4495 1
Size: 346 449
Fragments arranged by size:
1967 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 1554 1554
Size: 4943
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2893 3074 3237 2893
Size: 181 163 4599
Fragments arranged by size:
4599 181 163
BtsI GCAGTG_nn'
Cuts at: 2186 2186
Size: 4943
DraI TTT'AAA
Cuts at: 213 362 1649 1942 213
Size: 149 1287 293 3214
Fragments arranged by size:
3214 1287 293 149
DrdI GACnn_nn'nnGTC
Cuts at: 2649 3507 2649
Size: 858 4085
EaeI y'GGCC_r
Cuts at: 2528 2702 3093 3120 4465 2528
Size: 174 391 27 1345 3006
Fragments arranged by size:
3006 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2528 2528
Size: 4943
EarI CTCTTCn'nnn_
Cuts at: 416 974 1808 2966 3176 416
Size: 558 834 1158 210 2183
Fragments arranged by size:
2183 1158 834 558 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 979 3151 3461 3607 4753 979
Size: 2172 310 146 1146 1169
Fragments arranged by size:
2172 1169 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1563 1955 1563
Size: 392 4551
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1442 1683 2768 3200 3947 4215 1442
Size: 241 1085 432 747 268 2170
Fragments arranged by size:
2170 1085 747 432 268 241
EcoNI CCTnn'n_nnAGG
Cuts at: 611 611
Size: 4943
EcoO109I rG'GnC_Cy
Cuts at: 2082 2082
Size: 4943
EcoRI G'AATT_C
Cuts at: 202 1947 202
Size: 1745 3198
EcoRV GAT'ATC
Cuts at: 494 494
Size: 4943
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2962 2962
Size: 4943
FspI TGC'GCA
Cuts at: 2724 2724
Size: 4943
HaeII r_GCGC'y
Cuts at: 2377 2625 3647 4626 2377
Size: 248 1022 979 2694
Fragments arranged by size:
2694 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 712 744 805 837 1589 1621 1840 1872 2785
Size: 32 61 32 752 32 219 32 913
Cuts at: 2785 2815 2817 2847 712
Size: 30 2 30 2808
Fragments arranged by size:
2808 913 752 219 61 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 818 1886 1982 818
Size: 1068 96 3779
Fragments arranged by size:
3779 1068 96
KasI G'GCGC_C
Cuts at: 2373 2621 2373
Size: 248 4695
KpnI G_GTAC'C
Cuts at: 1963 1963
Size: 4943
MluI A'CGCG_T
Cuts at: 4376 4376
Size: 4943
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 317 355 3614 3798 317
Size: 38 3259 184 1462
Fragments arranged by size:
3259 1462 184 38
MscI TGG'CCA
Cuts at: 2704 2704
Size: 4943
MslI CAynn'nnrTG
Cuts at: 125 224 1109 1166 1182 1250 1505 1521 1676
Size: 99 885 57 16 68 255 16 155
Cuts at: 1676 2932 3059 125
Size: 1256 127 2009
Fragments arranged by size:
2009 1256 885 255 155 127 99 68
57 16 16
MspA1I CmG'CkG
Cuts at: 159 1140 2054 2368 2728 3741 3986 159
Size: 981 914 314 360 1013 245 1116
Fragments arranged by size:
1116 1013 981 914 360 314 245
NaeI GCC'GGC
Cuts at: 3124 3124
Size: 4943
NarI GG'CG_CC
Cuts at: 2374 2622 2374
Size: 248 4695
NdeI CA'TA_TG
Cuts at: 1350 1522 1350
Size: 172 4771
NgoMIV G'CCGG_C
Cuts at: 3122 3122
Size: 4943
NsiI A_TGCA'T
Cuts at: 729 1247 729
Size: 518 4425
NspI r_CATG'y
Cuts at: 1028 1153 1245 1833 1996 2496 3403 1028
Size: 125 92 588 163 500 907 2568
Fragments arranged by size:
2568 907 588 500 163 125 92
PciI A'CATG_T
Cuts at: 1024 3399 1024
Size: 2375 2568
PmeI GTTT'AAAC
Cuts at: 1942 1942
Size: 4943
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4082 4114 4082
Size: 32 4911
PsiI TTA'TAA
Cuts at: 557 557
Size: 4943
PspXI vC'TCGA_Gb
Cuts at: 811 811
Size: 4943
PstI C_TGCA'G
Cuts at: 1990 2675 1990
Size: 685 4258
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4943
PvuII CAG'CTG
Cuts at: 1140 2368 2728 1140
Size: 1228 360 3355
Fragments arranged by size:
3355 1228 360
RsrII CG'GwC_CG
Cuts at: 3138 3138
Size: 4943
SacI G_AGCT'C
Cuts at: 1565 1957 1565
Size: 392 4551
SalI G'TCGA_C
Cuts at: 1980 1980
Size: 4943
SapI GCTCTTCn'nnn_
Cuts at: 974 1808 2966 3176 974
Size: 834 1158 210 2741
Fragments arranged by size:
2741 1158 834 210
SbfI CC_TGCA'GG
Cuts at: 1990 1990
Size: 4943
ScaI AGT'ACT
Cuts at: 4460 4460
Size: 4943
SfcI C'TryA_G
Cuts at: 33 264 485 584 770 1986 2671 3664 3855
Size: 231 221 99 186 1216 685 993 191
Cuts at: 3855 33
Size: 1121
Fragments arranged by size:
1216 1121 993 685 231 221 191 186
99
SfoI GGC'GCC
Cuts at: 2375 2623 2375
Size: 248 4695
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4943
SmaI CCC'GGG
Cuts at: 1965 1965
Size: 4943
SmlI C'TyrA_G
Cuts at: 811 1312 1382 2098 3013 3505 3767 811
Size: 501 70 716 915 492 262 1987
Fragments arranged by size:
1987 915 716 501 492 262 70
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4943
SphI G_CATG'C
Cuts at: 1245 1996 2496 1245
Size: 751 500 3692
Fragments arranged by size:
3692 751 500
StyI C'CwwG_G
Cuts at: 1001 2077 2431 1001
Size: 1076 354 3513
Fragments arranged by size:
3513 1076 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1568 2526 2659 3118 3327 1568
Size: 958 133 459 209 3184
Fragments arranged by size:
3184 958 459 209 133
TatI w'GTAC_w
Cuts at: 4458 4458
Size: 4943
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1320 1352 1564 1596 4460 4492 1320
Size: 32 212 32 2864 32 1771
Fragments arranged by size:
2864 1771 212 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2740 2740
Size: 4943
XbaI T'CTAG_A
Cuts at: 1692 1974 1692
Size: 282 4661
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2438 2438
Size: 4943
XhoI C'TCGA_G
Cuts at: 811 811
Size: 4943
XmaI C'CCGG_G
Cuts at: 1963 1963
Size: 4943
XmnI GAAnn'nnTTC
Cuts at: 273 661 273
Size: 388 4555
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI Bsu36I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NdeI NgoMIV NsiI NspI PciI PmeI PpiI
PsiI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BaeI BbvCI BglI BmgBI BmtI Bpu10I
BseRI BsiWI BsmBI BsrGI BssHII BstEII BstZ17I BtgI
ClaI CspCI DraIII FseI FspAI HindIII HpaI MfeI
NcoI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI