Restriction map for
pF1KB7830
(Circular) (Six-base) MAPSORT of: pF1KB7830.seq Check: 8946 from: 1 to: 4928
pF1KB7830 4928 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1966 1966
Size: 4928
Acc65I G'GTAC_C
Cuts at: 1944 1944
Size: 4928
AclI AA'CG_TT
Cuts at: 4768 4768
Size: 4928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 594 1011 1091 1263 1515 2753 3185 3932 4200
Size: 417 80 172 252 1238 432 747 268
Cuts at: 4200 594
Size: 1322
Fragments arranged by size:
1322 1238 747 432 417 268 252 172
80
AflII C'TTAA_G
Cuts at: 1446 1446
Size: 4928
AflIII A'CryG_T
Cuts at: 1768 1852 2280 3384 4361 1768
Size: 84 428 1104 977 2335
Fragments arranged by size:
2335 1104 977 428 84
AgeI A'CCGG_T
Cuts at: 3316 3316
Size: 4928
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1131 1131
Size: 4928
AlwNI CAG_nnn'CTG
Cuts at: 426 2233 3800 426
Size: 1807 1567 1554
Fragments arranged by size:
1807 1567 1554
ApaLI G'TGCA_C
Cuts at: 3698 3698
Size: 4928
ApoI r'AATT_y
Cuts at: 702 1061 1145 1229 1313 1481 1649 1817 1901
Size: 359 84 84 84 168 168 168 84
Cuts at: 1901 1932 4281 4537 702
Size: 31 2349 256 1093
Fragments arranged by size:
2349 1093 359 256 168 168 168 84
84 84 84 31
AseI AT'TA_AT
Cuts at: 20 20
Size: 4928
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
AvaI C'yCGr_G
Cuts at: 179 1948 179
Size: 1769 3159
BamHI G'GATC_C
Cuts at: 1953 1953
Size: 4928
BanI G'GyrC_C
Cuts at: 1944 2358 2606 2641 1944
Size: 414 248 35 4231
Fragments arranged by size:
4231 414 248 35
BanII G_rGCy'C
Cuts at: 272 1942 2972 272
Size: 1670 1030 2228
Fragments arranged by size:
2228 1670 1030
BbeI G_GCGC'C
Cuts at: 2362 2610 2362
Size: 248 4680
BciVI GTATCCnnnnn_n'
Cuts at: 2826 3587 4928 2826
Size: 761 1341 2826
Fragments arranged by size:
2826 1341 761
BclI T'GATC_A
Cuts at: 2448 2448
Size: 4928
BfrBI ATG'CAT
Cuts at: 1569 1569
Size: 4928
BglII A'GATC_T
Cuts at: 2443 4480 2443
Size: 2037 2891
BlpI GC'TnA_GC
Cuts at: 2040 2040
Size: 4928
Bme1580I G_kGCm'C
Cuts at: 2553 2646 3702 2553
Size: 93 1056 3779
Fragments arranged by size:
3779 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 274 2140 2221 2556 4700 274
Size: 1866 81 335 2144 502
Fragments arranged by size:
2144 1866 502 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1310 1562 3295 1310
Size: 252 1733 2943
Fragments arranged by size:
2943 1733 252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 923 1259 1427 1511 1595 1679 1847 259
Size: 664 336 168 84 84 84 168 3340
Fragments arranged by size:
3340 664 336 168 168 84 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2104 2983 3475 3773 2104
Size: 879 492 298 3259
Fragments arranged by size:
3259 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 4632 264
Size: 4368 560
BsaAI yAC'GTr
Cuts at: 1801 2281 1801
Size: 480 4448
BsaBI GATnn'nnATC
Cuts at: 6 2442 4479 6
Size: 2436 2037 455
Fragments arranged by size:
2436 2037 455
BsaHI Gr'CG_yC
Cuts at: 350 2359 2607 3309 4805 350
Size: 2009 248 702 1496 473
Fragments arranged by size:
2009 1496 702 473 248
BsaWI w'CCGG_w
Cuts at: 1321 2117 2638 3316 3590 3737 4148 1321
Size: 796 521 678 274 147 411 2101
Fragments arranged by size:
2101 796 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 254 284 254
Size: 30 4898
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 345 345
Size: 4928
BseYI C'CCAG_C
Cuts at: 115 2353 3111 3688 4184 115
Size: 2238 758 577 496 859
Fragments arranged by size:
2238 859 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2470 2516 3724 104
Size: 2366 46 1208 1308
Fragments arranged by size:
2366 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1942 2720 2910 3702 1942
Size: 778 190 792 3168
Fragments arranged by size:
3168 792 778 190
BsmI GAATG_Cn'
Cuts at: 1571 1571
Size: 4928
BsmBI CGTCTCn'nnnn_
Cuts at: 357 357
Size: 4928
Bsp1286I G_dGCh'C
Cuts at: 272 1942 2553 2646 2720 2910 2972 3702 272
Size: 1670 611 93 74 190 62 730 1498
Fragments arranged by size:
1670 1498 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4148 4148
Size: 4928
BspHI T'CATG_A
Cuts at: 4104 4104
Size: 4928
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1978 2192 2494 2875 1978
Size: 214 302 381 4031
Fragments arranged by size:
4031 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3220 3274 4758 4923 50
Size: 3170 54 1484 165 55
Fragments arranged by size:
3170 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1350 2840 1350
Size: 1490 3438
BsrFI r'CCGG_y
Cuts at: 2926 3107 3316 2926
Size: 181 209 4538
Fragments arranged by size:
4538 209 181
BssSI C'ACGA_G
Cuts at: 1474 3199 3557 1474
Size: 1725 358 2845
Fragments arranged by size:
2845 1725 358
BstAPI GCAn_nnn'nTGC
Cuts at: 2305 2305
Size: 4928
BstBI TT'CG_AA
Cuts at: 3289 3289
Size: 4928
BstYI r'GATC_y
Cuts at: 1 318 439 1953 2420 2443 2778 3024 4036
Size: 317 121 1514 467 23 335 246 1012
Cuts at: 4036 4122 4134 4480 1
Size: 86 12 346 449
Fragments arranged by size:
1514 1012 467 449 346 335 317 246
121 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4928
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2878 3059 3222 2878
Size: 181 163 4584
Fragments arranged by size:
4584 181 163
BtsI GCAGTG_nn'
Cuts at: 2171 2171
Size: 4928
ClaI AT'CG_AT
Cuts at: 1674 1674
Size: 4928
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1332 1367 1332
Size: 35 4893
DraI TTT'AAA
Cuts at: 1254 1758 1927 1254
Size: 504 169 4255
Fragments arranged by size:
4255 504 169
DrdI GACnn_nn'nnGTC
Cuts at: 2634 3492 2634
Size: 858 4070
EaeI y'GGCC_r
Cuts at: 1701 2513 2687 3078 3105 4450 1701
Size: 812 174 391 27 1345 2179
Fragments arranged by size:
2179 1345 812 391 174 27
EagI C'GGCC_G
Cuts at: 2513 2513
Size: 4928
EarI CTCTTCn'nnn_
Cuts at: 364 417 1907 2951 3161 364
Size: 53 1490 1044 210 2131
Fragments arranged by size:
2131 1490 1044 210 53
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3136 3446 3592 4738 3136
Size: 310 146 1146 3326
Fragments arranged by size:
3326 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1940 1940
Size: 4928
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 594 923 1011 1091 1259 1263 1427 1511
Size: 335 329 88 80 168 4 164 84
Cuts at: 1511 1515 1595 1679 1847 2753 3185 3932 4200
Size: 4 80 84 168 906 432 747 268
Cuts at: 4200 259
Size: 987
Fragments arranged by size:
987 906 747 432 335 329 268 168
168 164 88 84 84 80 80 4
4
EcoO109I rG'GnC_Cy
Cuts at: 134 1917 2067 134
Size: 1783 150 2995
Fragments arranged by size:
2995 1783 150
EcoRI G'AATT_C
Cuts at: 702 1145 1229 1313 1481 1649 1817 1932 702
Size: 443 84 84 168 168 168 115 3698
Fragments arranged by size:
3698 443 168 168 168 115 84 84
EcoRV GAT'ATC
Cuts at: 1694 1694
Size: 4928
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1185 2947 1185
Size: 1762 3166
FspI TGC'GCA
Cuts at: 2709 2709
Size: 4928
HaeII r_GCGC'y
Cuts at: 2362 2610 3632 4611 2362
Size: 248 1022 979 2679
Fragments arranged by size:
2679 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1379 1411 2770 2800 2802 2832 1379
Size: 32 1359 30 2 30 3475
Fragments arranged by size:
3475 1359 32 30 30 2
HincII GTy'rAC
Cuts at: 395 1967 395
Size: 1572 3356
KasI G'GCGC_C
Cuts at: 2358 2606 2358
Size: 248 4680
KpnI G_GTAC'C
Cuts at: 1948 1948
Size: 4928
MluI A'CGCG_T
Cuts at: 4361 4361
Size: 4928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 467 3599 3783 467
Size: 3132 184 1612
Fragments arranged by size:
3132 1612 184
MscI TGG'CCA
Cuts at: 1703 2689 1703
Size: 986 3942
MslI CAynn'nnrTG
Cuts at: 669 1506 1617 1674 2917 3044 669
Size: 837 111 57 1243 127 2553
Fragments arranged by size:
2553 1243 837 127 111 57
MspA1I CmG'CkG
Cuts at: 123 2039 2353 2713 3726 3971 123
Size: 1916 314 360 1013 245 1080
Fragments arranged by size:
1916 1080 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3109 3109
Size: 4928
NarI GG'CG_CC
Cuts at: 2359 2607 2359
Size: 248 4680
NcoI C'CATG_G
Cuts at: 107 107
Size: 4928
NdeI CA'TA_TG
Cuts at: 919 919
Size: 4928
NgoMIV G'CCGG_C
Cuts at: 3107 3107
Size: 4928
NsiI A_TGCA'T
Cuts at: 1571 1571
Size: 4928
NspI r_CATG'y
Cuts at: 1772 1856 1981 2481 3388 1772
Size: 84 125 500 907 3312
Fragments arranged by size:
3312 907 500 125 84
PasI CC'CwG_GG
Cuts at: 323 323
Size: 4928
PciI A'CATG_T
Cuts at: 1768 1852 3384 1768
Size: 84 1532 3312
Fragments arranged by size:
3312 1532 84
PflMI CCAn_nnn'nTGG
Cuts at: 987 987
Size: 4928
PmeI GTTT'AAAC
Cuts at: 1927 1927
Size: 4928
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 244 276 4067 4099 244
Size: 32 3791 32 1073
Fragments arranged by size:
3791 1073 32 32
PpuMI rG'GwC_Cy
Cuts at: 1917 1917
Size: 4928
PsiI TTA'TAA
Cuts at: 965 1421 1898 965
Size: 456 477 3995
Fragments arranged by size:
3995 477 456
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 831 863 831
Size: 32 4896
PstI C_TGCA'G
Cuts at: 1975 2660 1975
Size: 685 4243
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4928
PvuII CAG'CTG
Cuts at: 2353 2713 2353
Size: 360 4568
RsrII CG'GwC_CG
Cuts at: 3123 3123
Size: 4928
SacI G_AGCT'C
Cuts at: 1942 1942
Size: 4928
SalI G'TCGA_C
Cuts at: 1965 1965
Size: 4928
SapI GCTCTTCn'nnn_
Cuts at: 364 2951 3161 364
Size: 2587 210 2131
Fragments arranged by size:
2587 2131 210
SbfI CC_TGCA'GG
Cuts at: 1975 1975
Size: 4928
ScaI AGT'ACT
Cuts at: 4445 4445
Size: 4928
SexAI A'CCwGG_T
Cuts at: 254 254
Size: 4928
SfcI C'TryA_G
Cuts at: 33 246 569 999 1335 1971 2656 3649 3840
Size: 213 323 430 336 636 685 993 191
Cuts at: 3840 33
Size: 1121
Fragments arranged by size:
1121 993 685 636 430 336 323 213
191
SfoI GGC'GCC
Cuts at: 2360 2608 2360
Size: 248 4680
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4928
SmaI CCC'GGG
Cuts at: 1950 1950
Size: 4928
SmlI C'TyrA_G
Cuts at: 1446 2083 2998 3490 3752 1446
Size: 637 915 492 262 2622
Fragments arranged by size:
2622 915 637 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4928
SphI G_CATG'C
Cuts at: 1981 2481 1981
Size: 500 4428
StuI AGG'CCT
Cuts at: 1043 1043
Size: 4928
StyI C'CwwG_G
Cuts at: 107 457 2062 2416 107
Size: 350 1605 354 2619
Fragments arranged by size:
2619 1605 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 565 2511 2644 3103 3312 565
Size: 1946 133 459 209 2181
Fragments arranged by size:
2181 1946 459 209 133
TatI w'GTAC_w
Cuts at: 952 4443 952
Size: 3491 1437
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 254 286 4445 4477 254
Size: 32 4159 32 705
Fragments arranged by size:
4159 705 32 32
Tth111I GACn'n_nGTC
Cuts at: 2725 2725
Size: 4928
XbaI T'CTAG_A
Cuts at: 1153 1959 1153
Size: 806 4122
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2423 2423
Size: 4928
XmaI C'CCGG_G
Cuts at: 1948 1948
Size: 4928
XmnI GAAnn'nnTTC
Cuts at: 1149 1313 1612 1864 1149
Size: 164 299 252 4213
Fragments arranged by size:
4213 299 252 164
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NsiI NspI PasI PciI
PflMI PmeI PpiI PpuMI PsiI PsrI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AfeI AhdI AleI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsrGI BssHII BstEII BstXI BstZ17I
Bsu36I DraIII EcoNI FseI FspAI HindIII HpaI MfeI
NheI NotI NruI PacI PfoI PmlI PshAI PspOMI
PspXI SacII SanDI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI