Restriction map for
pF1KB7824
(Circular) (Six-base) MAPSORT of: pF1KB7824.seq Check: 333 from: 1 to: 4925
pF1KB7824 4925 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1012 1655 1963 1012
Size: 643 308 3974
Fragments arranged by size:
3974 643 308
Acc65I G'GTAC_C
Cuts at: 1941 1941
Size: 4925
AclI AA'CG_TT
Cuts at: 4765 4765
Size: 4925
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 588 713 990 1242 2750 3182 3929 4197 588
Size: 125 277 252 1508 432 747 268 1316
Fragments arranged by size:
1508 1316 747 432 277 268 252 125
AflIII A'CryG_T
Cuts at: 2277 3381 4358 2277
Size: 1104 977 2844
Fragments arranged by size:
2844 1104 977
AgeI A'CCGG_T
Cuts at: 3313 3313
Size: 4925
AlwNI CAG_nnn'CTG
Cuts at: 736 2230 3797 736
Size: 1494 1567 1864
Fragments arranged by size:
1864 1567 1494
ApaLI G'TGCA_C
Cuts at: 3695 3695
Size: 4925
ApoI r'AATT_y
Cuts at: 379 587 1929 4278 4534 379
Size: 208 1342 2349 256 770
Fragments arranged by size:
2349 1342 770 256 208
AseI AT'TA_AT
Cuts at: 20 20
Size: 4925
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
AvaI C'yCGr_G
Cuts at: 963 1945 963
Size: 982 3943
BamHI G'GATC_C
Cuts at: 1950 1950
Size: 4925
BanI G'GyrC_C
Cuts at: 1367 1941 2355 2603 2638 1367
Size: 574 414 248 35 3654
Fragments arranged by size:
3654 574 414 248 35
BanII G_rGCy'C
Cuts at: 1210 1939 2969 1210
Size: 729 1030 3166
Fragments arranged by size:
3166 1030 729
BbeI G_GCGC'C
Cuts at: 2359 2607 2359
Size: 248 4677
BbsI GAAGACnn'nnnn_
Cuts at: 1908 1908
Size: 4925
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1324 1358 1324
Size: 34 4891
BciVI GTATCCnnnnn_n'
Cuts at: 2823 3584 4925 2823
Size: 761 1341 2823
Fragments arranged by size:
2823 1341 761
BclI T'GATC_A
Cuts at: 2445 2445
Size: 4925
BglII A'GATC_T
Cuts at: 464 2440 4477 464
Size: 1976 2037 912
Fragments arranged by size:
2037 1976 912
BlpI GC'TnA_GC
Cuts at: 2037 2037
Size: 4925
Bme1580I G_kGCm'C
Cuts at: 820 1337 1408 1492 1576 2550 2643 3699 820
Size: 517 71 84 84 974 93 1056 2046
Fragments arranged by size:
2046 1056 974 517 93 84 84 71
BmrI ACTGGGnnnn_n'
Cuts at: 619 775 1404 2137 2218 2553 4697 619
Size: 156 629 733 81 335 2144 847
Fragments arranged by size:
2144 847 733 629 335 156 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 224 3292 224
Size: 3068 1857
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 325 758 1180 259
Size: 66 433 422 4004
Fragments arranged by size:
4004 433 422 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1261 2101 2980 3472 3770 1261
Size: 840 879 492 298 2416
Fragments arranged by size:
2416 879 840 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 264 4629 264
Size: 4365 560
BsaAI yAC'GTr
Cuts at: 751 1653 2278 751
Size: 902 625 3398
Fragments arranged by size:
3398 902 625
BsaBI GATnn'nnATC
Cuts at: 6 2439 4476 6
Size: 2433 2037 455
Fragments arranged by size:
2433 2037 455
BsaHI Gr'CG_yC
Cuts at: 915 2356 2604 3306 4802 915
Size: 1441 248 702 1496 1038
Fragments arranged by size:
1496 1441 1038 702 248
BsaWI w'CCGG_w
Cuts at: 2114 2635 3313 3587 3734 4145 2114
Size: 521 678 274 147 411 2894
Fragments arranged by size:
2894 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 104 134 961 991 1801 1831 104
Size: 30 827 30 810 30 3198
Fragments arranged by size:
3198 827 810 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 346 418 1150 1183 1264 1600 1654 1657 346
Size: 72 732 33 81 336 54 3 3614
Fragments arranged by size:
3614 732 336 81 72 54 33 3
BseYI C'CCAG_C
Cuts at: 2350 3108 3685 4181 2350
Size: 758 577 496 3094
Fragments arranged by size:
3094 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1005 1005
Size: 4925
BsiEI CG_ry'CG
Cuts at: 104 2467 2513 3721 104
Size: 2363 46 1208 1308
Fragments arranged by size:
2363 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1210 1939 2717 2907 3699 1210
Size: 729 778 190 792 2436
Fragments arranged by size:
2436 792 778 729 190
BsmI GAATG_Cn'
Cuts at: 829 829
Size: 4925
Bsp1286I G_dGCh'C
Cuts at: 820 1210 1337 1408 1492 1576 1939 2550 2643
Size: 390 127 71 84 84 363 611 93
Cuts at: 2643 2717 2907 2969 3699 820
Size: 74 190 62 730 2046
Fragments arranged by size:
2046 730 611 390 363 190 127 93
84 84 74 71 62
BspEI T'CCGG_A
Cuts at: 4145 4145
Size: 4925
BspHI T'CATG_A
Cuts at: 4101 4101
Size: 4925
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1975 2189 2491 2872 1975
Size: 214 302 381 4028
Fragments arranged by size:
4028 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3217 3271 4755 4920 50
Size: 3167 54 1484 165 55
Fragments arranged by size:
3167 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2837 2837
Size: 4925
BsrFI r'CCGG_y
Cuts at: 2923 3104 3313 2923
Size: 181 209 4535
Fragments arranged by size:
4535 209 181
BsrGI T'GTAC_A
Cuts at: 221 221
Size: 4925
BssSI C'ACGA_G
Cuts at: 3196 3554 3196
Size: 358 4567
BstAPI GCAn_nnn'nTGC
Cuts at: 2302 2302
Size: 4925
BstBI TT'CG_AA
Cuts at: 3286 3286
Size: 4925
BstEII G'GTnAC_C
Cuts at: 619 1437 1521 619
Size: 818 84 4023
Fragments arranged by size:
4023 818 84
BstXI CCAn_nnnn'nTGG
Cuts at: 632 632
Size: 4925
BstYI r'GATC_y
Cuts at: 1 464 1950 2417 2440 2775 3021 4033 4119
Size: 463 1486 467 23 335 246 1012 86
Cuts at: 4119 4131 4477 1
Size: 12 346 449
Fragments arranged by size:
1486 1012 467 463 449 346 335 246
86 23 12
BstZ17I GTA'TAC
Cuts at: 1013 1013
Size: 4925
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1757 2875 3056 3219 1757
Size: 1118 181 163 3463
Fragments arranged by size:
3463 1118 181 163
BtsI GCAGTG_nn'
Cuts at: 2168 2168
Size: 4925
DraI TTT'AAA
Cuts at: 1924 1924
Size: 4925
DraIII CAC_nnn'GTG
Cuts at: 405 405
Size: 4925
DrdI GACnn_nn'nnGTC
Cuts at: 2631 3489 2631
Size: 858 4067
EaeI y'GGCC_r
Cuts at: 450 1680 2510 2684 3075 3102 4447 450
Size: 1230 830 174 391 27 1345 928
Fragments arranged by size:
1345 1230 928 830 391 174 27
EagI C'GGCC_G
Cuts at: 2510 2510
Size: 4925
EarI CTCTTCn'nnn_
Cuts at: 2948 3158 2948
Size: 210 4715
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3133 3443 3589 4735 3133
Size: 310 146 1146 3323
Fragments arranged by size:
3323 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1208 1937 1208
Size: 729 4196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 259 325 588 713 758 990 1180 1242 2750
Size: 66 263 125 45 232 190 62 1508
Cuts at: 2750 3182 3929 4197 259
Size: 432 747 268 987
Fragments arranged by size:
1508 987 747 432 268 263 232 190
125 66 62 45
EcoO109I rG'GnC_Cy
Cuts at: 215 553 1058 1226 2064 215
Size: 338 505 168 838 3076
Fragments arranged by size:
3076 838 505 338 168
EcoRI G'AATT_C
Cuts at: 379 1929 379
Size: 1550 3375
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1878 2944 1878
Size: 1066 3859
FspI TGC'GCA
Cuts at: 2706 2706
Size: 4925
HaeII r_GCGC'y
Cuts at: 2359 2607 3629 4608 2359
Size: 248 1022 979 2676
Fragments arranged by size:
2676 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2767 2797 2799 2829 2767
Size: 30 2 30 4863
Fragments arranged by size:
4863 30 30 2
HincII GTy'rAC
Cuts at: 1964 1964
Size: 4925
KasI G'GCGC_C
Cuts at: 2355 2603 2355
Size: 248 4677
KpnI G_GTAC'C
Cuts at: 1945 1945
Size: 4925
MluI A'CGCG_T
Cuts at: 4358 4358
Size: 4925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 522 1809 3596 3780 522
Size: 1287 1787 184 1667
Fragments arranged by size:
1787 1667 1287 184
MscI TGG'CCA
Cuts at: 452 1682 2686 452
Size: 1230 1004 2691
Fragments arranged by size:
2691 1230 1004
MslI CAynn'nnrTG
Cuts at: 617 897 2914 3041 617
Size: 280 2017 127 2501
Fragments arranged by size:
2501 2017 280 127
MspA1I CmG'CkG
Cuts at: 303 1794 2036 2350 2710 3723 3968 303
Size: 1491 242 314 360 1013 245 1260
Fragments arranged by size:
1491 1260 1013 360 314 245 242
NaeI GCC'GGC
Cuts at: 3106 3106
Size: 4925
NarI GG'CG_CC
Cuts at: 2356 2604 2356
Size: 248 4677
NgoMIV G'CCGG_C
Cuts at: 3104 3104
Size: 4925
NspI r_CATG'y
Cuts at: 1978 2478 3385 1978
Size: 500 907 3518
Fragments arranged by size:
3518 907 500
PasI CC'CwG_GG
Cuts at: 264 264
Size: 4925
PciI A'CATG_T
Cuts at: 3381 3381
Size: 4925
PflMI CCAn_nnn'nTGG
Cuts at: 405 405
Size: 4925
PfoI T'CCnGG_A
Cuts at: 155 155
Size: 4925
PmeI GTTT'AAAC
Cuts at: 1924 1924
Size: 4925
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4064 4096 4064
Size: 32 4893
PpuMI rG'GwC_Cy
Cuts at: 215 215
Size: 4925
PshAI GACnn'nnGTC
Cuts at: 356 356
Size: 4925
PstI C_TGCA'G
Cuts at: 486 1828 1972 2657 486
Size: 1342 144 685 2754
Fragments arranged by size:
2754 1342 685 144
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4925
PvuII CAG'CTG
Cuts at: 303 2350 2710 303
Size: 2047 360 2518
Fragments arranged by size:
2518 2047 360
RsrII CG'GwC_CG
Cuts at: 3120 3120
Size: 4925
SacI G_AGCT'C
Cuts at: 1210 1939 1210
Size: 729 4196
SalI G'TCGA_C
Cuts at: 1962 1962
Size: 4925
SapI GCTCTTCn'nnn_
Cuts at: 2948 3158 2948
Size: 210 4715
SbfI CC_TGCA'GG
Cuts at: 1972 1972
Size: 4925
ScaI AGT'ACT
Cuts at: 4442 4442
Size: 4925
SexAI A'CCwGG_T
Cuts at: 401 1286 401
Size: 885 4040
SfcI C'TryA_G
Cuts at: 33 246 482 759 1824 1968 2653 3646 3837
Size: 213 236 277 1065 144 685 993 191
Cuts at: 3837 33
Size: 1121
Fragments arranged by size:
1121 1065 993 685 277 236 213 191
144
SfoI GGC'GCC
Cuts at: 2357 2605 2357
Size: 248 4677
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4925
SmaI CCC'GGG
Cuts at: 1947 1947
Size: 4925
SmlI C'TyrA_G
Cuts at: 1240 2080 2995 3487 3749 1240
Size: 840 915 492 262 2416
Fragments arranged by size:
2416 915 840 492 262
SnaBI TAC'GTA
Cuts at: 1653 1653
Size: 4925
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4925
SphI G_CATG'C
Cuts at: 1978 2478 1978
Size: 500 4425
StuI AGG'CCT
Cuts at: 934 934
Size: 4925
StyI C'CwwG_G
Cuts at: 322 2059 2413 322
Size: 1737 354 2834
Fragments arranged by size:
2834 1737 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 193 2508 2641 3100 3309 193
Size: 2315 133 459 209 1809
Fragments arranged by size:
2315 1809 459 209 133
TatI w'GTAC_w
Cuts at: 221 4440 221
Size: 4219 706
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 104 136 452 484 4442 4474 104
Size: 32 316 32 3958 32 555
Fragments arranged by size:
3958 555 316 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1884 2722 1884
Size: 838 4087
XbaI T'CTAG_A
Cuts at: 1956 1956
Size: 4925
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2420 2420
Size: 4925
XmaI C'CCGG_G
Cuts at: 1945 1945
Size: 4925
XmnI GAAnn'nnTTC
Cuts at: 344 1144 344
Size: 800 4125
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgZI BtsI
DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SnaBI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BfrBI BglI BmgBI
BmtI Bpu10I BsiWI BsmBI BssHII Bsu36I BtgI ClaI
CspCI EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PmlI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SrfI SspI SwaI XhoI ZraI