Restriction map for
pF1KB7821
(Circular) (Six-base) MAPSORT of: pF1KB7821.seq Check: 4952 from: 1 to: 4721
pF1KB7821 4721 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1441 1759 1441
Size: 318 4403
Acc65I G'GTAC_C
Cuts at: 1737 1737
Size: 4721
AclI AA'CG_TT
Cuts at: 4561 4561
Size: 4721
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 827 1649 1655 2546 2978 3725 3993 827
Size: 822 6 891 432 747 268 1555
Fragments arranged by size:
1555 891 822 747 432 268 6
AflIII A'CryG_T
Cuts at: 2073 3177 4154 2073
Size: 1104 977 2640
Fragments arranged by size:
2640 1104 977
AgeI A'CCGG_T
Cuts at: 3109 3109
Size: 4721
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 254 254
Size: 4721
AlwNI CAG_nnn'CTG
Cuts at: 2026 3593 2026
Size: 1567 3154
ApaLI G'TGCA_C
Cuts at: 3491 3491
Size: 4721
ApoI r'AATT_y
Cuts at: 248 376 467 1725 4074 4330 248
Size: 128 91 1258 2349 256 639
Fragments arranged by size:
2349 1258 639 256 128 91
AseI AT'TA_AT
Cuts at: 20 20
Size: 4721
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
AvaI C'yCGr_G
Cuts at: 1741 1741
Size: 4721
BamHI G'GATC_C
Cuts at: 1746 1746
Size: 4721
BanI G'GyrC_C
Cuts at: 1737 2151 2399 2434 1737
Size: 414 248 35 4024
Fragments arranged by size:
4024 414 248 35
BanII G_rGCy'C
Cuts at: 729 1735 2765 729
Size: 1006 1030 2685
Fragments arranged by size:
2685 1030 1006
BbeI G_GCGC'C
Cuts at: 2155 2403 2155
Size: 248 4473
BbsI GAAGACnn'nnnn_
Cuts at: 1254 1254
Size: 4721
BbvCI CC'TCA_GC
Cuts at: 139 139
Size: 4721
BciVI GTATCCnnnnn_n'
Cuts at: 2619 3380 4721 2619
Size: 761 1341 2619
Fragments arranged by size:
2619 1341 761
BclI T'GATC_A
Cuts at: 2241 2241
Size: 4721
BglI GCCn_nnn'nGGC
Cuts at: 203 203
Size: 4721
BglII A'GATC_T
Cuts at: 1269 2236 4273 1269
Size: 967 2037 1717
Fragments arranged by size:
2037 1717 967
BlpI GC'TnA_GC
Cuts at: 1833 1833
Size: 4721
Bme1580I G_kGCm'C
Cuts at: 2346 2439 3495 2346
Size: 93 1056 3572
Fragments arranged by size:
3572 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 886 1933 2014 2349 4493 886
Size: 1047 81 335 2144 1114
Fragments arranged by size:
2144 1114 1047 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3088 3088
Size: 4721
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 1636 213
Size: 1423 3298
Bpu10I CC'TnA_GC
Cuts at: 139 139
Size: 4721
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1897 2776 3268 3566 1897
Size: 879 492 298 3052
Fragments arranged by size:
3052 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4425 4425
Size: 4721
BsaAI yAC'GTr
Cuts at: 2074 2074
Size: 4721
BsaBI GATnn'nnATC
Cuts at: 6 776 2235 4272 6
Size: 770 1459 2037 455
Fragments arranged by size:
2037 1459 770 455
BsaHI Gr'CG_yC
Cuts at: 2152 2400 3102 4598 2152
Size: 248 702 1496 2275
Fragments arranged by size:
2275 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1910 2431 3109 3383 3530 3941 1910
Size: 521 678 274 147 411 2690
Fragments arranged by size:
2690 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 2146 2904 3481 3977 2146
Size: 758 577 496 2890
Fragments arranged by size:
2890 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1321 1664 1321
Size: 343 4378
BsiEI CG_ry'CG
Cuts at: 104 2263 2309 3517 104
Size: 2159 46 1208 1308
Fragments arranged by size:
2159 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 507 1735 2513 2703 3495 507
Size: 1228 778 190 792 1733
Fragments arranged by size:
1733 1228 792 778 190
BsmI GAATG_Cn'
Cuts at: 1462 1462
Size: 4721
Bsp1286I G_dGCh'C
Cuts at: 507 729 1735 2346 2439 2513 2703 2765 3495
Size: 222 1006 611 93 74 190 62 730
Cuts at: 3495 507
Size: 1733
Fragments arranged by size:
1733 1006 730 611 222 190 93 74
62
BspEI T'CCGG_A
Cuts at: 3941 3941
Size: 4721
BspHI T'CATG_A
Cuts at: 3897 3897
Size: 4721
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1771 1985 2287 2668 1771
Size: 214 302 381 3824
Fragments arranged by size:
3824 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3013 3067 4551 4716 50
Size: 2963 54 1484 165 55
Fragments arranged by size:
2963 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2633 2633
Size: 4721
BsrFI r'CCGG_y
Cuts at: 129 2719 2900 3109 129
Size: 2590 181 209 1741
Fragments arranged by size:
2590 1741 209 181
BssSI C'ACGA_G
Cuts at: 2992 3350 2992
Size: 358 4363
BstAPI GCAn_nnn'nTGC
Cuts at: 610 2098 610
Size: 1488 3233
BstBI TT'CG_AA
Cuts at: 121 633 3082 121
Size: 512 2449 1760
Fragments arranged by size:
2449 1760 512
BstXI CCAn_nnnn'nTGG
Cuts at: 1096 1096
Size: 4721
BstYI r'GATC_y
Cuts at: 1 1122 1269 1746 2213 2236 2571 2817 3829
Size: 1121 147 477 467 23 335 246 1012
Cuts at: 3829 3915 3927 4273 1
Size: 86 12 346 449
Fragments arranged by size:
1121 1012 477 467 449 346 335 246
147 86 23 12
BstZ17I GTA'TAC
Cuts at: 1442 1442
Size: 4721
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2671 2852 3015 2671
Size: 181 163 4377
Fragments arranged by size:
4377 181 163
BtsI GCAGTG_nn'
Cuts at: 1059 1964 1059
Size: 905 3816
DraI TTT'AAA
Cuts at: 689 815 1720 689
Size: 126 905 3690
Fragments arranged by size:
3690 905 126
DrdI GACnn_nn'nnGTC
Cuts at: 2427 3285 2427
Size: 858 3863
EaeI y'GGCC_r
Cuts at: 204 2306 2480 2871 2898 4243 204
Size: 2102 174 391 27 1345 682
Fragments arranged by size:
2102 1345 682 391 174 27
EagI C'GGCC_G
Cuts at: 2306 2306
Size: 4721
EarI CTCTTCn'nnn_
Cuts at: 1388 2744 2954 1388
Size: 1356 210 3155
Fragments arranged by size:
3155 1356 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2929 3239 3385 4531 2929
Size: 310 146 1146 3119
Fragments arranged by size:
3119 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1733 1733
Size: 4721
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 213 827 1636 1649 1655 2546 2978 3725 3993
Size: 614 809 13 6 891 432 747 268
Cuts at: 3993 213
Size: 941
Fragments arranged by size:
941 891 809 747 614 432 268 13
6
EcoNI CCTnn'n_nnAGG
Cuts at: 580 580
Size: 4721
EcoO109I rG'GnC_Cy
Cuts at: 1860 1860
Size: 4721
EcoRI G'AATT_C
Cuts at: 1725 1725
Size: 4721
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 268 2740 268
Size: 2472 2249
FspI TGC'GCA
Cuts at: 2502 2502
Size: 4721
HaeII r_GCGC'y
Cuts at: 2155 2403 3425 4404 2155
Size: 248 1022 979 2472
Fragments arranged by size:
2472 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 556 588 907 939 2563 2593 2595 2625 556
Size: 32 319 32 1624 30 2 30 2652
Fragments arranged by size:
2652 1624 319 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1760 1760
Size: 4721
HindIII A'AGCT_T
Cuts at: 285 1603 285
Size: 1318 3403
KasI G'GCGC_C
Cuts at: 2151 2399 2151
Size: 248 4473
KpnI G_GTAC'C
Cuts at: 1741 1741
Size: 4721
MluI A'CGCG_T
Cuts at: 4154 4154
Size: 4721
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 398 959 1169 3392 3576 398
Size: 561 210 2223 184 1543
Fragments arranged by size:
2223 1543 561 210 184
MscI TGG'CCA
Cuts at: 206 2482 206
Size: 2276 2445
MslI CAynn'nnrTG
Cuts at: 2710 2837 2710
Size: 127 4594
MspA1I CmG'CkG
Cuts at: 1832 2146 2506 3519 3764 1832
Size: 314 360 1013 245 2789
Fragments arranged by size:
2789 1013 360 314 245
NaeI GCC'GGC
Cuts at: 131 2902 131
Size: 2771 1950
NarI GG'CG_CC
Cuts at: 2152 2400 2152
Size: 248 4473
NgoMIV G'CCGG_C
Cuts at: 129 2900 129
Size: 2771 1950
NspI r_CATG'y
Cuts at: 613 1774 2274 3181 613
Size: 1161 500 907 2153
Fragments arranged by size:
2153 1161 907 500
PciI A'CATG_T
Cuts at: 3177 3177
Size: 4721
PmeI GTTT'AAAC
Cuts at: 1720 1720
Size: 4721
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3892 3860
Size: 32 4689
PsiI TTA'TAA
Cuts at: 496 496
Size: 4721
PstI C_TGCA'G
Cuts at: 1216 1768 2453 1216
Size: 552 685 3484
Fragments arranged by size:
3484 685 552
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4721
PvuII CAG'CTG
Cuts at: 2146 2506 2146
Size: 360 4361
RsrII CG'GwC_CG
Cuts at: 2916 2916
Size: 4721
SacI G_AGCT'C
Cuts at: 1735 1735
Size: 4721
SalI G'TCGA_C
Cuts at: 1758 1758
Size: 4721
SapI GCTCTTCn'nnn_
Cuts at: 2744 2954 2744
Size: 210 4511
SbfI CC_TGCA'GG
Cuts at: 1768 1768
Size: 4721
ScaI AGT'ACT
Cuts at: 4238 4238
Size: 4721
SfcI C'TryA_G
Cuts at: 33 371 939 1212 1535 1764 2449 3442 3633
Size: 338 568 273 323 229 685 993 191
Cuts at: 3633 33
Size: 1121
Fragments arranged by size:
1121 993 685 568 338 323 273 229
191
SfoI GGC'GCC
Cuts at: 2153 2401 2153
Size: 248 4473
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4721
SmaI CCC'GGG
Cuts at: 1743 1743
Size: 4721
SmlI C'TyrA_G
Cuts at: 1876 2791 3283 3545 1876
Size: 915 492 262 3052
Fragments arranged by size:
3052 915 492 262
SpeI A'CTAG_T
Cuts at: 67 1291 67
Size: 1224 3497
SphI G_CATG'C
Cuts at: 613 1774 2274 613
Size: 1161 500 3060
Fragments arranged by size:
3060 1161 500
SspI AAT'ATT
Cuts at: 262 262
Size: 4721
StuI AGG'CCT
Cuts at: 702 702
Size: 4721
StyI C'CwwG_G
Cuts at: 820 1855 2209 820
Size: 1035 354 3332
Fragments arranged by size:
3332 1035 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2304 2437 2896 3105 2304
Size: 133 459 209 3920
Fragments arranged by size:
3920 459 209 133
TatI w'GTAC_w
Cuts at: 327 1504 4236 327
Size: 1177 2732 812
Fragments arranged by size:
2732 1177 812
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4238 4270 4238
Size: 32 4689
Tth111I GACn'n_nGTC
Cuts at: 2518 2518
Size: 4721
XbaI T'CTAG_A
Cuts at: 1752 1752
Size: 4721
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2216 2216
Size: 4721
XmaI C'CCGG_G
Cuts at: 1741 1741
Size: 4721
XmnI GAAnn'nnTTC
Cuts at: 251 251
Size: 4721
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlfI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BstZ17I BtgZI BtsI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PciI PmeI PpiI PsiI PstI
PvuI PvuII RsrII SacI SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmgBI BmtI BsaXI
BseRI BsiWI BsmBI BsrGI BssHII BstEII Bsu36I BtgI
ClaI CspCI DraIII EcoRV FseI FspAI HpaI MfeI
NcoI NdeI NheI NotI NruI NsiI PacI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PspXI PsrI
SacII SanDI SexAI SfiI SgrAI SnaBI SrfI SwaI
XhoI ZraI