Restriction map for
pF1KB7812
(Circular) (Six-base) MAPSORT of: pF1KB7812.seq Check: 3553 from: 1 to: 4898
pF1KB7812 4898 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 429 1936 429
Size: 1507 3391
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 1559 2723 3155 3902 4170 172
Size: 1387 1164 432 747 268 900
Fragments arranged by size:
1387 1164 900 747 432 268
AflIII A'CryG_T
Cuts at: 1723 2250 3354 4331 1723
Size: 527 1104 977 2290
Fragments arranged by size:
2290 1104 977 527
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 2203 3770 2203
Size: 1567 3331
ApaLI G'TGCA_C
Cuts at: 3668 3668
Size: 4898
ApoI r'AATT_y
Cuts at: 142 186 438 577 585 689 705 773 857
Size: 44 252 139 8 104 16 68 84
Cuts at: 857 1025 1445 1697 1781 1902 4251 4507 142
Size: 168 420 252 84 121 2349 256 533
Fragments arranged by size:
2349 533 420 256 252 252 168 139
121 104 84 84 68 44 16 8
AseI AT'TA_AT
Cuts at: 20 448 20
Size: 428 4470
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 356 1918 356
Size: 1562 3336
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 1914 2328 2576 2611 1914
Size: 414 248 35 4201
Fragments arranged by size:
4201 414 248 35
BanII G_rGCy'C
Cuts at: 1912 2942 1912
Size: 1030 3868
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbsI GAAGACnn'nnnn_
Cuts at: 222 222
Size: 4898
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3557 4898 2796
Size: 761 1341 2796
Fragments arranged by size:
2796 1341 761
BclI T'GATC_A
Cuts at: 263 2418 263
Size: 2155 2743
BglII A'GATC_T
Cuts at: 2413 4450 2413
Size: 2037 2861
BlpI GC'TnA_GC
Cuts at: 2010 2010
Size: 4898
Bme1580I G_kGCm'C
Cuts at: 2523 2616 3672 2523
Size: 93 1056 3749
Fragments arranged by size:
3749 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 2110 2191 2526 4670 2110
Size: 81 335 2144 2338
Fragments arranged by size:
2338 2144 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 295 315 887 971 1055 1139 1223 1307 1391
Size: 20 572 84 84 84 84 84 84
Cuts at: 1391 1475 1643 1727 1811 295
Size: 84 168 84 84 3382
Fragments arranged by size:
3382 572 168 84 84 84 84 84
84 84 84 84 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3445 3743 2074
Size: 879 492 298 3229
Fragments arranged by size:
3229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 353 4602 353
Size: 4249 649
BsaAI yAC'GTr
Cuts at: 2251 2251
Size: 4898
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3279 4775 2329
Size: 248 702 1496 2452
Fragments arranged by size:
2452 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1873 2087 2608 3286 3560 3707 4118 1873
Size: 214 521 678 274 147 411 2653
Fragments arranged by size:
2653 678 521 411 274 214 147
BseYI C'CCAG_C
Cuts at: 2323 3081 3658 4154 2323
Size: 758 577 496 3067
Fragments arranged by size:
3067 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 161 1154 161
Size: 993 3905
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1912 2690 2880 3672 1912
Size: 778 190 792 3138
Fragments arranged by size:
3138 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1912 2523 2616 2690 2880 2942 3672 1912
Size: 611 93 74 190 62 730 3138
Fragments arranged by size:
3138 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4118 4118
Size: 4898
BspHI T'CATG_A
Cuts at: 4074 4074
Size: 4898
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1948 2162 2464 2845 1948
Size: 214 302 381 4001
Fragments arranged by size:
4001 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 169 2810 169
Size: 2641 2257
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BssSI C'ACGA_G
Cuts at: 3169 3527 3169
Size: 358 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 2275 2275
Size: 4898
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstYI r'GATC_y
Cuts at: 1 1923 2390 2413 2748 2994 4006 4092 4104
Size: 1922 467 23 335 246 1012 86 12
Cuts at: 4104 4450 1
Size: 346 449
Fragments arranged by size:
1922 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2848 3029 3192 2848
Size: 181 163 4554
Fragments arranged by size:
4554 181 163
BtsI GCAGTG_nn'
Cuts at: 173 2141 173
Size: 1968 2930
DraI TTT'AAA
Cuts at: 1329 1581 1833 1897 1329
Size: 252 252 64 4330
Fragments arranged by size:
4330 252 252 64
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3462 2604
Size: 858 4040
EaeI y'GGCC_r
Cuts at: 134 323 367 2483 2657 3048 3075 4420 134
Size: 189 44 2116 174 391 27 1345 612
Fragments arranged by size:
2116 1345 612 391 189 174 44 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 298 1341 1535 2921 3131 298
Size: 1043 194 1386 210 2065
Fragments arranged by size:
2065 1386 1043 210 194
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3416 3562 4708 3106
Size: 310 146 1146 3296
Fragments arranged by size:
3296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 172 295 315 887 971 1055 1139 1223 1307
Size: 123 20 572 84 84 84 84 84
Cuts at: 1307 1391 1475 1559 1643 1727 1811 2723 3155
Size: 84 84 84 84 84 84 912 432
Cuts at: 3155 3902 4170 172
Size: 747 268 900
Fragments arranged by size:
912 900 747 572 432 268 123 84
84 84 84 84 84 84 84 84
84 84 20
EcoO109I rG'GnC_Cy
Cuts at: 2037 2037
Size: 4898
EcoRI G'AATT_C
Cuts at: 142 689 773 1781 1902 142
Size: 547 84 1008 121 3138
Fragments arranged by size:
3138 1008 547 121 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4898
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 2332 2580 3602 4581 2332
Size: 248 1022 979 2649
Fragments arranged by size:
2649 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 255 287 299 331 2740 2770 2772 2802 255
Size: 32 12 32 2409 30 2 30 2351
Fragments arranged by size:
2409 2351 32 32 30 30 12 2
HincII GTy'rAC
Cuts at: 1937 1937
Size: 4898
HindIII A'AGCT_T
Cuts at: 986 1322 1406 1490 1658 1826 986
Size: 336 84 84 168 168 4058
Fragments arranged by size:
4058 336 168 168 84 84
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3569 3753 3569
Size: 184 4714
MscI TGG'CCA
Cuts at: 136 325 2659 136
Size: 189 2334 2375
Fragments arranged by size:
2375 2334 189
MslI CAynn'nnrTG
Cuts at: 337 1638 2887 3014 337
Size: 1301 1249 127 2221
Fragments arranged by size:
2221 1301 1249 127
MspA1I CmG'CkG
Cuts at: 183 2009 2323 2683 3696 3941 183
Size: 1826 314 360 1013 245 1140
Fragments arranged by size:
1826 1140 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NcoI C'CATG_G
Cuts at: 107 107
Size: 4898
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NspI r_CATG'y
Cuts at: 1727 1951 2451 3358 1727
Size: 224 500 907 3267
Fragments arranged by size:
3267 907 500 224
PciI A'CATG_T
Cuts at: 1723 3354 1723
Size: 1631 3267
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4866
PstI C_TGCA'G
Cuts at: 1945 2630 1945
Size: 685 4213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 2323 2683 2323
Size: 360 4538
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 2921 3131 2921
Size: 210 4688
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SexAI A'CCwGG_T
Cuts at: 224 224
Size: 4898
SfcI C'TryA_G
Cuts at: 33 216 1941 2626 3619 3810 33
Size: 183 1725 685 993 191 1121
Fragments arranged by size:
1725 1121 993 685 191 183
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 2053 2968 3460 3722 2053
Size: 915 492 262 3229
Fragments arranged by size:
3229 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
SspI AAT'ATT
Cuts at: 542 542
Size: 4898
StuI AGG'CCT
Cuts at: 292 292
Size: 4898
StyI C'CwwG_G
Cuts at: 107 2032 2386 107
Size: 1925 354 2619
Fragments arranged by size:
2619 1925 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2481 2614 3073 3282 2481
Size: 133 459 209 4097
Fragments arranged by size:
4097 459 209 133
TatI w'GTAC_w
Cuts at: 4413 4413
Size: 4898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 4866
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2393 2393
Size: 4898
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 625 625
Size: 4898
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BciVI BclI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PmeI PpiI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SexAI SfcI SfoI SgfI SmaI SmlI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbvCI BcgI BfrBI BglI
BmgBI BmtI Bpu10I BsaXI BseRI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PfoI
PmlI PpuMI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SfiI SgrAI SnaBI SrfI SwaI XhoI ZraI