Restriction map for
pF1KB7810
(Circular) (Six-base) MAPSORT of: pF1KB7810.seq Check: 182 from: 1 to: 4898
pF1KB7810 4898 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 477 477
Size: 4898
AccI GT'mk_AC
Cuts at: 323 1936 323
Size: 1613 3285
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 4738 4738
Size: 4898
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 668 1101 1525 2723 3155 3902 4170 668
Size: 433 424 1198 432 747 268 1396
Fragments arranged by size:
1396 1198 747 433 432 424 268
AflIII A'CryG_T
Cuts at: 2250 3354 4331 2250
Size: 1104 977 2817
Fragments arranged by size:
2817 1104 977
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 1617 2203 3770 1617
Size: 586 1567 2745
Fragments arranged by size:
2745 1567 586
ApaLI G'TGCA_C
Cuts at: 786 3668 786
Size: 2882 2016
ApoI r'AATT_y
Cuts at: 564 1902 4251 4507 564
Size: 1338 2349 256 955
Fragments arranged by size:
2349 1338 955 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 271 352 1918 271
Size: 81 1566 3251
Fragments arranged by size:
3251 1566 81
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 277 489 1197 1707 1914 2328 2576 2611 277
Size: 212 708 510 207 414 248 35 2564
Fragments arranged by size:
2564 708 510 414 248 212 207 35
BanII G_rGCy'C
Cuts at: 182 427 515 1079 1825 1912 2942 182
Size: 245 88 564 746 87 1030 2138
Fragments arranged by size:
2138 1030 746 564 245 88 87
BbeI G_GCGC'C
Cuts at: 281 2332 2580 281
Size: 2051 248 2599
Fragments arranged by size:
2599 2051 248
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1179 1213 1179
Size: 34 4864
BciVI GTATCCnnnnn_n'
Cuts at: 863 2796 3557 4898 863
Size: 1933 761 1341 863
Fragments arranged by size:
1933 1341 863 761
BclI T'GATC_A
Cuts at: 998 1178 1227 2418 998
Size: 180 49 1191 3478
Fragments arranged by size:
3478 1191 180 49
BglI GCCn_nnn'nGGC
Cuts at: 587 587
Size: 4898
BglII A'GATC_T
Cuts at: 1376 2413 4450 1376
Size: 1037 2037 1824
Fragments arranged by size:
2037 1824 1037
BlpI GC'TnA_GC
Cuts at: 1073 2010 1073
Size: 937 3961
Bme1580I G_kGCm'C
Cuts at: 494 790 1712 2523 2616 3672 494
Size: 296 922 811 93 1056 1720
Fragments arranged by size:
1720 1056 922 811 296 93
BmrI ACTGGGnnnn_n'
Cuts at: 1194 2110 2191 2526 4670 1194
Size: 916 81 335 2144 1422
Fragments arranged by size:
2144 1422 916 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3265 3265
Size: 4898
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 232 523 977 1496 1750 196
Size: 36 291 454 519 254 3344
Fragments arranged by size:
3344 519 454 291 254 36
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 184 2074 2953 3445 3743 184
Size: 1890 879 492 298 1339
Fragments arranged by size:
1890 1339 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1282 4602 1282
Size: 3320 1578
BsaAI yAC'GTr
Cuts at: 2251 2251
Size: 4898
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 278 579 1742 2329 2577 3279 4775 278
Size: 301 1163 587 248 702 1496 401
Fragments arranged by size:
1496 1163 702 587 401 301 248
BsaWI w'CCGG_w
Cuts at: 2087 2608 3286 3560 3707 4118 2087
Size: 521 678 274 147 411 2867
Fragments arranged by size:
2867 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1429 1459 1666 1696 1429
Size: 30 207 30 4631
Fragments arranged by size:
4631 207 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 902 1450 1618 1621 1804 902
Size: 548 168 3 183 3996
Fragments arranged by size:
3996 548 183 168 3
BseYI C'CCAG_C
Cuts at: 514 1622 2323 3081 3658 4154 514
Size: 1108 701 758 577 496 1258
Fragments arranged by size:
1258 1108 758 701 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 424 683 424
Size: 259 4639
BsiEI CG_ry'CG
Cuts at: 104 305 1043 1892 2440 2486 3694 104
Size: 201 738 849 548 46 1208 1308
Fragments arranged by size:
1308 1208 849 738 548 201 46
BsiHKAI G_wGCw'C
Cuts at: 790 1301 1912 2690 2880 3672 790
Size: 511 611 778 190 792 2016
Fragments arranged by size:
2016 792 778 611 511 190
BsmI GAATG_Cn'
Cuts at: 550 1836 550
Size: 1286 3612
BsmBI CGTCTCn'nnnn_
Cuts at: 1786 1786
Size: 4898
Bsp1286I G_dGCh'C
Cuts at: 182 427 494 515 790 1079 1301 1712 1825
Size: 245 67 21 275 289 222 411 113
Cuts at: 1825 1912 2523 2616 2690 2880 2942 3672 182
Size: 87 611 93 74 190 62 730 1408
Fragments arranged by size:
1408 730 611 411 289 275 245 222
190 113 93 87 74 67 62 21
BspEI T'CCGG_A
Cuts at: 4118 4118
Size: 4898
BspHI T'CATG_A
Cuts at: 995 4074 995
Size: 3079 1819
BspMI ACCTGCnnnn'nnnn_
Cuts at: 309 477 1730 1948 2162 2464 2845 309
Size: 168 1253 218 214 302 381 2362
Fragments arranged by size:
2362 1253 381 302 218 214 168
BsrBI CCG'CTC
Cuts at: 50 216 993 3190 3244 4728 4893 50
Size: 166 777 2197 54 1484 165 55
Fragments arranged by size:
2197 1484 777 166 165 55 54
BsrDI GCAATG_nn'
Cuts at: 678 2810 678
Size: 2132 2766
BsrFI r'CCGG_y
Cuts at: 334 538 546 827 2896 3077 3286 334
Size: 204 8 281 2069 181 209 1946
Fragments arranged by size:
2069 1946 281 209 204 181 8
BsrGI T'GTAC_A
Cuts at: 260 1481 1682 260
Size: 1221 201 3476
Fragments arranged by size:
3476 1221 201
BssSI C'ACGA_G
Cuts at: 3169 3527 3169
Size: 358 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 1694 1842 2275 1694
Size: 148 433 4317
Fragments arranged by size:
4317 433 148
BstBI TT'CG_AA
Cuts at: 3259 3259
Size: 4898
BstXI CCAn_nnnn'nTGG
Cuts at: 387 387
Size: 4898
BstYI r'GATC_y
Cuts at: 1 158 203 1262 1376 1923 2390 2413 2748
Size: 157 45 1059 114 547 467 23 335
Cuts at: 2748 2994 4006 4092 4104 4450 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1059 1012 547 467 449 346 335 246
157 114 86 45 23 12
BtgI C'CryG_G
Cuts at: 299 477 1292 1770 1784 1886 299
Size: 178 815 478 14 102 3311
Fragments arranged by size:
3311 815 478 178 102 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1817 2848 3029 3192 1817
Size: 1031 181 163 3523
Fragments arranged by size:
3523 1031 181 163
BtsI GCAGTG_nn'
Cuts at: 671 1814 2141 671
Size: 1143 327 3428
Fragments arranged by size:
3428 1143 327
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 1051 2604 3462 1051
Size: 1553 858 2487
Fragments arranged by size:
2487 1553 858
EaeI y'GGCC_r
Cuts at: 302 480 602 982 1040 1577 1811 2483 2657
Size: 178 122 380 58 537 234 672 174
Cuts at: 2657 3048 3075 4420 302
Size: 391 27 1345 780
Fragments arranged by size:
1345 780 672 537 391 380 234 178
174 122 58 27
EagI C'GGCC_G
Cuts at: 302 1040 2483 302
Size: 738 1443 2717
Fragments arranged by size:
2717 1443 738
EarI CTCTTCn'nnn_
Cuts at: 805 879 1186 1511 2921 3131 805
Size: 74 307 325 1410 210 2572
Fragments arranged by size:
2572 1410 325 307 210 74
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3416 3562 4708 3106
Size: 310 146 1146 3296
Fragments arranged by size:
3296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 232 523 668 977 1101 1496 1525 1750
Size: 36 291 145 309 124 395 29 225
Cuts at: 1750 2723 3155 3902 4170 196
Size: 973 432 747 268 924
Fragments arranged by size:
973 924 747 432 395 309 291 268
225 145 124 36 29
EcoNI CCTnn'n_nnAGG
Cuts at: 226 226
Size: 4898
EcoO109I rG'GnC_Cy
Cuts at: 2037 2037
Size: 4898
EcoRI G'AATT_C
Cuts at: 1902 1902
Size: 4898
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 747 2917 747
Size: 2170 2728
FseI GG_CCGG'CC
Cuts at: 542 542
Size: 4898
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 281 915 1290 2332 2580 3602 4581 281
Size: 634 375 1042 248 1022 979 598
Fragments arranged by size:
1042 1022 979 634 598 375 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1260 1292 2740 2770 2772 2802 1260
Size: 32 1448 30 2 30 3356
Fragments arranged by size:
3356 1448 32 30 30 2
HincII GTy'rAC
Cuts at: 1937 1937
Size: 4898
HindIII A'AGCT_T
Cuts at: 1124 1124
Size: 4898
KasI G'GCGC_C
Cuts at: 277 2328 2576 277
Size: 2051 248 2599
Fragments arranged by size:
2599 2051 248
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 404 1046 3569 3753 404
Size: 642 2523 184 1549
Fragments arranged by size:
2523 1549 642 184
MscI TGG'CCA
Cuts at: 604 984 1579 1813 2659 604
Size: 380 595 234 846 2843
Fragments arranged by size:
2843 846 595 380 234
MslI CAynn'nnrTG
Cuts at: 112 2887 3014 112
Size: 2775 127 1996
Fragments arranged by size:
2775 1996 127
MspA1I CmG'CkG
Cuts at: 301 429 456 518 1614 2009 2323 2683 3696
Size: 128 27 62 1096 395 314 360 1013
Cuts at: 3696 3941 301
Size: 245 1258
Fragments arranged by size:
1258 1096 1013 395 360 314 245 128
62 27
NaeI GCC'GGC
Cuts at: 540 548 829 3079 540
Size: 8 281 2250 2359
Fragments arranged by size:
2359 2250 281 8
NarI GG'CG_CC
Cuts at: 278 2329 2577 278
Size: 2051 248 2599
Fragments arranged by size:
2599 2051 248
NcoI C'CATG_G
Cuts at: 1292 1292
Size: 4898
NgoMIV G'CCGG_C
Cuts at: 538 546 827 3077 538
Size: 8 281 2250 2359
Fragments arranged by size:
2359 2250 281 8
NotI GC'GGCC_GC
Cuts at: 302 302
Size: 4898
NspI r_CATG'y
Cuts at: 1389 1759 1951 2451 3358 1389
Size: 370 192 500 907 2929
Fragments arranged by size:
2929 907 500 370 192
PasI CC'CwG_GG
Cuts at: 222 1303 222
Size: 1081 3817
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PflMI CCAn_nnn'nTGG
Cuts at: 980 1575 1617 980
Size: 595 42 4261
Fragments arranged by size:
4261 595 42
PfoI T'CCnGG_A
Cuts at: 136 1541 136
Size: 1405 3493
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 4037
Size: 32 4866
PstI C_TGCA'G
Cuts at: 473 1430 1601 1945 2630 473
Size: 957 171 344 685 2741
Fragments arranged by size:
2741 957 685 344 171
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 456 2323 2683 456
Size: 1867 360 2671
Fragments arranged by size:
2671 1867 360
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SacII CC_GC'GG
Cuts at: 302 302
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 805 2921 3131 805
Size: 2116 210 2572
Fragments arranged by size:
2572 2116 210
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SfcI C'TryA_G
Cuts at: 33 469 555 1426 1597 1941 2626 3619 3810
Size: 436 86 871 171 344 685 993 191
Cuts at: 3810 33
Size: 1121
Fragments arranged by size:
1121 993 871 685 436 344 191 171
86
SfoI GGC'GCC
Cuts at: 279 2330 2578 279
Size: 2051 248 2599
Fragments arranged by size:
2599 2051 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 354 1920 354
Size: 1566 3332
SmlI C'TyrA_G
Cuts at: 199 2053 2968 3460 3722 199
Size: 1854 915 492 262 1375
Fragments arranged by size:
1854 1375 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StuI AGG'CCT
Cuts at: 727 823 1589 727
Size: 96 766 4036
Fragments arranged by size:
4036 766 96
StyI C'CwwG_G
Cuts at: 162 644 1292 1580 2032 2386 162
Size: 482 648 288 452 354 2674
Fragments arranged by size:
2674 648 482 452 354 288
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 896 1316 2481 2614 3073 3282 896
Size: 420 1165 133 459 209 2512
Fragments arranged by size:
2512 1165 459 420 209 133
TatI w'GTAC_w
Cuts at: 260 1481 1682 4413 260
Size: 1221 201 2731 745
Fragments arranged by size:
2731 1221 745 201
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1429 1461 4415 4447 1429
Size: 32 2954 32 1880
Fragments arranged by size:
2954 1880 32 32
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1242 1929 1242
Size: 687 4211
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 922 2393 922
Size: 1471 3427
XmaI C'CCGG_G
Cuts at: 352 1918 352
Size: 1566 3332
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BcgI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI
Bpu10I BsiWI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FspAI HpaI MfeI NdeI NheI NruI
NsiI PacI PmlI PpuMI PshAI PsiI PspOMI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI