Restriction map for
pF1KB7808
(Circular) (Six-base) MAPSORT of: pF1KB7808.seq Check: 4104 from: 1 to: 4898
pF1KB7808 4898 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 924 1008 1936 924
Size: 84 928 3886
Fragments arranged by size:
3886 928 84
Acc65I G'GTAC_C
Cuts at: 1914 1914
Size: 4898
AclI AA'CG_TT
Cuts at: 1578 4738 1578
Size: 3160 1738
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 431 549 1305 1389 1577 1661 1741 1745
Size: 152 118 756 84 188 84 80 4
Cuts at: 1745 2723 3155 3902 4170 279
Size: 978 432 747 268 1007
Fragments arranged by size:
1007 978 756 747 432 268 188 152
118 84 84 80 4
AflIII A'CryG_T
Cuts at: 988 1095 2250 3354 4331 988
Size: 107 1155 1104 977 1555
Fragments arranged by size:
1555 1155 1104 977 107
AgeI A'CCGG_T
Cuts at: 3286 3286
Size: 4898
AlwNI CAG_nnn'CTG
Cuts at: 2203 3770 2203
Size: 1567 3331
ApaLI G'TGCA_C
Cuts at: 3668 3668
Size: 4898
ApoI r'AATT_y
Cuts at: 767 1164 1187 1867 1902 4251 4507 767
Size: 397 23 680 35 2349 256 1158
Fragments arranged by size:
2349 1158 680 397 256 35 23
AseI AT'TA_AT
Cuts at: 20 20
Size: 4898
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
AvaI C'yCGr_G
Cuts at: 1918 1918
Size: 4898
AvrII C'CTAG_G
Cuts at: 453 453
Size: 4898
BamHI G'GATC_C
Cuts at: 1923 1923
Size: 4898
BanI G'GyrC_C
Cuts at: 1914 2328 2576 2611 1914
Size: 414 248 35 4201
Fragments arranged by size:
4201 414 248 35
BanII G_rGCy'C
Cuts at: 1912 2942 1912
Size: 1030 3868
BbeI G_GCGC'C
Cuts at: 2332 2580 2332
Size: 248 4650
BbsI GAAGACnn'nnnn_
Cuts at: 678 678
Size: 4898
BciVI GTATCCnnnnn_n'
Cuts at: 2796 3557 4898 2796
Size: 761 1341 2796
Fragments arranged by size:
2796 1341 761
BclI T'GATC_A
Cuts at: 2418 2418
Size: 4898
BfrBI ATG'CAT
Cuts at: 1703 1821 1703
Size: 118 4780
BglII A'GATC_T
Cuts at: 1324 1764 2413 4450 1324
Size: 440 649 2037 1772
Fragments arranged by size:
2037 1772 649 440
BlpI GC'TnA_GC
Cuts at: 2010 2010
Size: 4898
Bme1580I G_kGCm'C
Cuts at: 2523 2616 3672 2523
Size: 93 1056 3749
Fragments arranged by size:
3749 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 109 358 2110 2191 2526 4670 109
Size: 249 1752 81 335 2144 337
Fragments arranged by size:
2144 1752 337 335 249 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1576 3265 1576
Size: 1689 3209
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 629 797 881 1133 1301 1385 1487 1571 1657
Size: 168 84 252 168 84 102 84 86
Cuts at: 1657 629
Size: 3870
Fragments arranged by size:
3870 252 168 168 102 86 84 84
84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2074 2953 3445 3743 2074
Size: 879 492 298 3229
Fragments arranged by size:
3229 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4602 4602
Size: 4898
BsaAI yAC'GTr
Cuts at: 989 1098 1788 2251 989
Size: 109 690 463 3636
Fragments arranged by size:
3636 690 463 109
BsaBI GATnn'nnATC
Cuts at: 6 2412 4449 6
Size: 2406 2037 455
Fragments arranged by size:
2406 2037 455
BsaHI Gr'CG_yC
Cuts at: 2329 2577 3279 4775 2329
Size: 248 702 1496 2452
Fragments arranged by size:
2452 1496 702 248
BsaWI w'CCGG_w
Cuts at: 2087 2608 3286 3560 3707 4118 2087
Size: 521 678 274 147 411 2867
Fragments arranged by size:
2867 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 562 592 562
Size: 30 4868
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 169 169
Size: 4898
BseYI C'CCAG_C
Cuts at: 2323 3081 3658 4154 2323
Size: 758 577 496 3067
Fragments arranged by size:
3067 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2440 2486 3694 104
Size: 2336 46 1208 1308
Fragments arranged by size:
2336 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 275 1912 2690 2880 3672 275
Size: 1637 778 190 792 1501
Fragments arranged by size:
1637 1501 792 778 190
BsmI GAATG_Cn'
Cuts at: 982 1573 1705 982
Size: 591 132 4175
Fragments arranged by size:
4175 591 132
Bsp1286I G_dGCh'C
Cuts at: 275 1912 2523 2616 2690 2880 2942 3672 275
Size: 1637 611 93 74 190 62 730 1501
Fragments arranged by size:
1637 1501 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 4118 4118
Size: 4898
BspHI T'CATG_A
Cuts at: 1263 1431 1619 1871 4074 1263
Size: 168 188 252 2203 2087
Fragments arranged by size:
2203 2087 252 188 168
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1948 2162 2464 2845 1948
Size: 214 302 381 4001
Fragments arranged by size:
4001 381 302 214
BsrBI CCG'CTC
Cuts at: 50 3190 3244 4728 4893 50
Size: 3140 54 1484 165 55
Fragments arranged by size:
3140 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1312 1668 1752 2810 1312
Size: 356 84 1058 3400
Fragments arranged by size:
3400 1058 356 84
BsrFI r'CCGG_y
Cuts at: 2896 3077 3286 2896
Size: 181 209 4508
Fragments arranged by size:
4508 209 181
BssSI C'ACGA_G
Cuts at: 3169 3527 3169
Size: 358 4540
BstAPI GCAn_nnn'nTGC
Cuts at: 2275 2275
Size: 4898
BstBI TT'CG_AA
Cuts at: 669 1865 3259 669
Size: 1196 1394 2308
Fragments arranged by size:
2308 1394 1196
BstYI r'GATC_y
Cuts at: 1 913 1324 1764 1848 1923 2390 2413 2748
Size: 912 411 440 84 75 467 23 335
Cuts at: 2748 2994 4006 4092 4104 4450 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 912 467 449 440 411 346 335
246 86 84 75 23 12
BstZ17I GTA'TAC
Cuts at: 1009 1009
Size: 4898
BtgI C'CryG_G
Cuts at: 107 107
Size: 4898
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2848 3029 3192 2848
Size: 181 163 4554
Fragments arranged by size:
4554 181 163
BtsI GCAGTG_nn'
Cuts at: 1060 1228 2141 1060
Size: 168 913 3817
Fragments arranged by size:
3817 913 168
DraI TTT'AAA
Cuts at: 1897 1897
Size: 4898
DrdI GACnn_nn'nnGTC
Cuts at: 2604 3462 2604
Size: 858 4040
EaeI y'GGCC_r
Cuts at: 2483 2657 3048 3075 4420 2483
Size: 174 391 27 1345 2961
Fragments arranged by size:
2961 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2483 2483
Size: 4898
EarI CTCTTCn'nnn_
Cuts at: 1717 2921 3131 1717
Size: 1204 210 3484
Fragments arranged by size:
3484 1204 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 3106 3416 3562 4708 3106
Size: 310 146 1146 3296
Fragments arranged by size:
3296 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1910 1910
Size: 4898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 279 431 549 629 797 881 1133 1301 1305
Size: 152 118 80 168 84 252 168 4
Cuts at: 1305 1385 1389 1487 1571 1577 1657 1661 1741
Size: 80 4 98 84 6 80 4 80
Cuts at: 1741 1745 2723 3155 3902 4170 279
Size: 4 978 432 747 268 1007
Fragments arranged by size:
1007 978 747 432 268 252 168 168
152 118 98 84 84 80 80 80
80 6 4 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 1019 2037 1019
Size: 1018 3880
EcoRI G'AATT_C
Cuts at: 767 1164 1867 1902 767
Size: 397 703 35 3763
Fragments arranged by size:
3763 703 397 35
EcoRV GAT'ATC
Cuts at: 1261 1617 1261
Size: 356 4542
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2917 2917
Size: 4898
FspI TGC'GCA
Cuts at: 2679 2679
Size: 4898
HaeII r_GCGC'y
Cuts at: 2332 2580 3602 4581 2332
Size: 248 1022 979 2649
Fragments arranged by size:
2649 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2740 2770 2772 2802 2740
Size: 30 2 30 4836
Fragments arranged by size:
4836 30 30 2
HincII GTy'rAC
Cuts at: 547 1937 547
Size: 1390 3508
HindIII A'AGCT_T
Cuts at: 401 429 401
Size: 28 4870
KasI G'GCGC_C
Cuts at: 2328 2576 2328
Size: 248 4650
KpnI G_GTAC'C
Cuts at: 1918 1918
Size: 4898
MluI A'CGCG_T
Cuts at: 4331 4331
Size: 4898
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3569 3753 3569
Size: 184 4714
MscI TGG'CCA
Cuts at: 2659 2659
Size: 4898
MslI CAynn'nnrTG
Cuts at: 540 662 682 792 987 2887 3014 540
Size: 122 20 110 195 1900 127 2424
Fragments arranged by size:
2424 1900 195 127 122 110 20
MspA1I CmG'CkG
Cuts at: 2009 2323 2683 3696 3941 2009
Size: 314 360 1013 245 2966
Fragments arranged by size:
2966 1013 360 314 245
NaeI GCC'GGC
Cuts at: 3079 3079
Size: 4898
NarI GG'CG_CC
Cuts at: 2329 2577 2329
Size: 248 4650
NcoI C'CATG_G
Cuts at: 107 107
Size: 4898
NdeI CA'TA_TG
Cuts at: 507 507
Size: 4898
NgoMIV G'CCGG_C
Cuts at: 3077 3077
Size: 4898
NsiI A_TGCA'T
Cuts at: 1705 1823 1705
Size: 118 4780
NspI r_CATG'y
Cuts at: 1825 1951 2451 3358 1825
Size: 126 500 907 3365
Fragments arranged by size:
3365 907 500 126
PciI A'CATG_T
Cuts at: 3354 3354
Size: 4898
PfoI T'CCnGG_A
Cuts at: 389 389
Size: 4898
PmeI GTTT'AAAC
Cuts at: 1897 1897
Size: 4898
PmlI CAC'GTG
Cuts at: 989 989
Size: 4898
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 214 246 4037 4069 214
Size: 32 3791 32 1043
Fragments arranged by size:
3791 1043 32 32
PpuMI rG'GwC_Cy
Cuts at: 1019 1019
Size: 4898
PsiI TTA'TAA
Cuts at: 707 1481 707
Size: 774 4124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 1589 1621 1589
Size: 32 4866
PstI C_TGCA'G
Cuts at: 1945 2630 1945
Size: 685 4213
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4898
PvuII CAG'CTG
Cuts at: 2323 2683 2323
Size: 360 4538
RsrII CG'GwC_CG
Cuts at: 3093 3093
Size: 4898
SacI G_AGCT'C
Cuts at: 1912 1912
Size: 4898
SalI G'TCGA_C
Cuts at: 1935 1935
Size: 4898
SapI GCTCTTCn'nnn_
Cuts at: 2921 3131 2921
Size: 210 4688
SbfI CC_TGCA'GG
Cuts at: 1945 1945
Size: 4898
ScaI AGT'ACT
Cuts at: 4415 4415
Size: 4898
SfcI C'TryA_G
Cuts at: 33 192 233 1941 2626 3619 3810 33
Size: 159 41 1708 685 993 191 1121
Fragments arranged by size:
1708 1121 993 685 191 159 41
SfoI GGC'GCC
Cuts at: 2330 2578 2330
Size: 248 4650
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4898
SmaI CCC'GGG
Cuts at: 1920 1920
Size: 4898
SmlI C'TyrA_G
Cuts at: 2053 2968 3460 3722 2053
Size: 915 492 262 3229
Fragments arranged by size:
3229 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4898
SphI G_CATG'C
Cuts at: 1951 2451 1951
Size: 500 4398
StyI C'CwwG_G
Cuts at: 107 453 2032 2386 107
Size: 346 1579 354 2619
Fragments arranged by size:
2619 1579 354 346
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 2481 2614 3073 3282 163
Size: 2318 133 459 209 1779
Fragments arranged by size:
2318 1779 459 209 133
TatI w'GTAC_w
Cuts at: 4413 4413
Size: 4898
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4415 4447 4415
Size: 32 4866
Tth111I GACn'n_nGTC
Cuts at: 2695 2695
Size: 4898
XbaI T'CTAG_A
Cuts at: 1929 1929
Size: 4898
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 2393 114
Size: 2279 2619
XmaI C'CCGG_G
Cuts at: 1918 1918
Size: 4898
XmnI GAAnn'nnTTC
Cuts at: 730 767 935 1187 1318 1402 1674 1758 1842
Size: 37 168 252 131 84 272 84 84
Cuts at: 1842 730
Size: 3786
Fragments arranged by size:
3786 272 252 168 131 84 84 84
37
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII
BbeI BbsI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI BstZ17I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI FspI HaeII Hin4I HincII HindIII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NsiI NspI PciI PfoI PmeI
PmlI PpiI PpuMI PsiI PsrI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI BaeI BbvCI BcgI BglI BmgBI BmtI
Bpu10I BsgI BsiWI BsmBI BsrGI BssHII BstEII BstXI
Bsu36I ClaI CspCI DraIII EcoNI FseI FspAI HpaI
MfeI NheI NotI NruI PacI PasI PflMI PshAI
PspOMI PspXI SacII SanDI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI ZraI