Restriction map for
pF1KB7801
(Circular) (Six-base) MAPSORT of: pF1KB7801.seq Check: 1888 from: 1 to: 4670
pF1KB7801 4670 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 710 1246 710
Size: 536 4134
AatII G_ACGT'C
Cuts at: 322 322
Size: 4670
AccI GT'mk_AC
Cuts at: 454 1708 454
Size: 1254 3416
Acc65I G'GTAC_C
Cuts at: 446 1686 446
Size: 1240 3430
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 768 1009 1141 1159 2495 2927 3674 3942 768
Size: 241 132 18 1336 432 747 268 1496
Fragments arranged by size:
1496 1336 747 432 268 241 132 18
AflIII A'CryG_T
Cuts at: 2022 3126 4103 2022
Size: 1104 977 2589
Fragments arranged by size:
2589 1104 977
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AleI CACnn'nnGTG
Cuts at: 737 737
Size: 4670
AlwNI CAG_nnn'CTG
Cuts at: 261 1590 1975 3542 261
Size: 1329 385 1567 1389
Fragments arranged by size:
1567 1389 1329 385
ApaI G_GGCC'C
Cuts at: 381 831 1304 381
Size: 450 473 3747
Fragments arranged by size:
3747 473 450
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 995 1674 4023 4279 995
Size: 679 2349 256 1386
Fragments arranged by size:
2349 1386 679 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 178 374 824 1434 1690 178
Size: 196 450 610 256 3158
Fragments arranged by size:
3158 610 450 256 196
BamHI G'GATC_C
Cuts at: 1695 1695
Size: 4670
BanI G'GyrC_C
Cuts at: 327 446 1234 1686 2100 2348 2383 327
Size: 119 788 452 414 248 35 2614
Fragments arranged by size:
2614 788 452 414 248 119 35
BanII G_rGCy'C
Cuts at: 179 381 831 1304 1358 1441 1684 2714 179
Size: 202 450 473 54 83 243 1030 2135
Fragments arranged by size:
2135 1030 473 450 243 202 83 54
BbeI G_GCGC'C
Cuts at: 331 2104 2352 331
Size: 1773 248 2649
Fragments arranged by size:
2649 1773 248
BbvCI CC'TCA_GC
Cuts at: 932 932
Size: 4670
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 2190 2190
Size: 4670
BglII A'GATC_T
Cuts at: 2185 4222 2185
Size: 2037 2633
BlpI GC'TnA_GC
Cuts at: 160 344 1782 160
Size: 184 1438 3048
Fragments arranged by size:
3048 1438 184
Bme1580I G_kGCm'C
Cuts at: 381 831 1304 2295 2388 3444 381
Size: 450 473 991 93 1056 1607
Fragments arranged by size:
1607 1056 991 473 450 93
BmrI ACTGGGnnnn_n'
Cuts at: 375 605 685 1882 1963 2298 4442 375
Size: 230 80 1197 81 335 2144 603
Fragments arranged by size:
2144 1197 603 335 230 81 80
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3037 3037
Size: 4670
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 661 795 828 1090 1150 1519 1576 1627
Size: 522 134 33 262 60 369 57 51
Cuts at: 1627 139
Size: 3182
Fragments arranged by size:
3182 522 369 262 134 60 57 51
33
Bpu10I CC'TnA_GC
Cuts at: 932 1028 1483 932
Size: 96 455 4119
Fragments arranged by size:
4119 455 96
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 2725 3217 3515 1846
Size: 879 492 298 3001
Fragments arranged by size:
3001 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1540 4374 1540
Size: 2834 1836
BsaAI yAC'GTr
Cuts at: 2023 2023
Size: 4670
BsaBI GATnn'nnATC
Cuts at: 6 1517 2184 4221 6
Size: 1511 667 2037 455
Fragments arranged by size:
2037 1511 667 455
BsaHI Gr'CG_yC
Cuts at: 319 328 2101 2349 3051 4547 319
Size: 9 1773 248 702 1496 442
Fragments arranged by size:
1773 1496 702 442 248 9
BsaWI w'CCGG_w
Cuts at: 1859 2380 3058 3332 3479 3890 1859
Size: 521 678 274 147 411 2639
Fragments arranged by size:
2639 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 340 370 793 823 1351 1381 340
Size: 30 423 30 528 30 3629
Fragments arranged by size:
3629 528 423 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 382 569 847 857 922 925 991 1435 382
Size: 187 278 10 65 3 66 444 3617
Fragments arranged by size:
3617 444 278 187 66 65 10 3
BseYI C'CCAG_C
Cuts at: 1333 1444 1452 1460 2095 2853 3430 3926 1333
Size: 111 8 8 635 758 577 496 2077
Fragments arranged by size:
2077 758 635 577 496 111 8 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 760 803 760
Size: 43 4627
BsiEI CG_ry'CG
Cuts at: 104 2212 2258 3466 104
Size: 2108 46 1208 1308
Fragments arranged by size:
2108 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 350 1684 2462 2652 3444 350
Size: 1334 778 190 792 1576
Fragments arranged by size:
1576 1334 792 778 190
BsmI GAATG_Cn'
Cuts at: 1273 1341 1273
Size: 68 4602
Bsp1286I G_dGCh'C
Cuts at: 179 350 381 831 1304 1358 1441 1684 2295
Size: 171 31 450 473 54 83 243 611
Cuts at: 2295 2388 2462 2652 2714 3444 179
Size: 93 74 190 62 730 1405
Fragments arranged by size:
1405 730 611 473 450 243 190 171
93 83 74 62 54 31
BspEI T'CCGG_A
Cuts at: 3890 3890
Size: 4670
BspHI T'CATG_A
Cuts at: 648 3846 648
Size: 3198 1472
BspMI ACCTGCnnnn'nnnn_
Cuts at: 710 1216 1246 1397 1720 1934 2236 2617 710
Size: 506 30 151 323 214 302 381 2763
Fragments arranged by size:
2763 506 381 323 302 214 151 30
BsrBI CCG'CTC
Cuts at: 50 125 2962 3016 4500 4665 50
Size: 75 2837 54 1484 165 55
Fragments arranged by size:
2837 1484 165 75 55 54
BsrDI GCAATG_nn'
Cuts at: 2582 2582
Size: 4670
BsrFI r'CCGG_y
Cuts at: 2668 2849 3058 2668
Size: 181 209 4280
Fragments arranged by size:
4280 209 181
BssSI C'ACGA_G
Cuts at: 609 2941 3299 609
Size: 2332 358 1980
Fragments arranged by size:
2332 1980 358
BstAPI GCAn_nnn'nTGC
Cuts at: 138 1347 2047 138
Size: 1209 700 2761
Fragments arranged by size:
2761 1209 700
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 561 1323 561
Size: 762 3908
BstYI r'GATC_y
Cuts at: 1 1378 1518 1695 2162 2185 2520 2766 3778
Size: 1377 140 177 467 23 335 246 1012
Cuts at: 3778 3864 3876 4222 1
Size: 86 12 346 449
Fragments arranged by size:
1377 1012 467 449 346 335 246 177
140 86 23 12
BtgI C'CryG_G
Cuts at: 107 1370 107
Size: 1263 3407
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2620 2801 2964 2620
Size: 181 163 4326
Fragments arranged by size:
4326 181 163
BtsI GCAGTG_nn'
Cuts at: 151 744 1913 151
Size: 593 1169 2908
Fragments arranged by size:
2908 1169 593
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 284 319 284
Size: 35 4635
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 2376 3234 2376
Size: 858 3812
EaeI y'GGCC_r
Cuts at: 2255 2429 2820 2847 4192 2255
Size: 174 391 27 1345 2733
Fragments arranged by size:
2733 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2255 2255
Size: 4670
EarI CTCTTCn'nnn_
Cuts at: 280 910 1641 2693 2903 280
Size: 630 731 1052 210 2047
Fragments arranged by size:
2047 1052 731 630 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 825 2878 3188 3334 4480 825
Size: 2053 310 146 1146 1015
Fragments arranged by size:
2053 1146 1015 310 146
EcoICRI GAG'CTC
Cuts at: 1682 1682
Size: 4670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 139 661 768 795 828 1009 1090 1141 1150
Size: 522 107 27 33 181 81 51 9
Cuts at: 1150 1159 1519 1576 1627 2495 2927 3674 3942
Size: 9 360 57 51 868 432 747 268
Cuts at: 3942 139
Size: 867
Fragments arranged by size:
868 867 747 522 432 360 268 181
107 81 57 51 51 33 27 9
9
EcoNI CCTnn'n_nnAGG
Cuts at: 364 904 364
Size: 540 4130
EcoO109I rG'GnC_Cy
Cuts at: 192 828 889 920 984 1301 1809 192
Size: 636 61 31 64 317 508 3053
Fragments arranged by size:
3053 636 508 317 64 61 31
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4670
FspI TGC'GCA
Cuts at: 2451 2451
Size: 4670
HaeII r_GCGC'y
Cuts at: 331 1601 2104 2352 3374 4353 331
Size: 1270 503 248 1022 979 648
Fragments arranged by size:
1270 1022 979 648 503 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1011 1043 1411 1443 2512 2542 2544 2574 1011
Size: 32 368 32 1069 30 2 30 3107
Fragments arranged by size:
3107 1069 368 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
HindIII A'AGCT_T
Cuts at: 1171 1171
Size: 4670
KasI G'GCGC_C
Cuts at: 327 2100 2348 327
Size: 1773 248 2649
Fragments arranged by size:
2649 1773 248
KpnI G_GTAC'C
Cuts at: 450 1690 450
Size: 1240 3430
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 542 3341 3525 542
Size: 2799 184 1687
Fragments arranged by size:
2799 1687 184
MscI TGG'CCA
Cuts at: 2431 2431
Size: 4670
MslI CAynn'nnrTG
Cuts at: 112 737 1249 1321 2659 2786 112
Size: 625 512 72 1338 127 1996
Fragments arranged by size:
1996 1338 625 512 127 72
MspA1I CmG'CkG
Cuts at: 1781 2095 2455 3468 3713 1781
Size: 314 360 1013 245 2738
Fragments arranged by size:
2738 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2851 2851
Size: 4670
NarI GG'CG_CC
Cuts at: 328 2101 2349 328
Size: 1773 248 2649
Fragments arranged by size:
2649 1773 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4670
NgoMIV G'CCGG_C
Cuts at: 2849 2849
Size: 4670
NspI r_CATG'y
Cuts at: 1723 2223 3130 1723
Size: 500 907 3263
Fragments arranged by size:
3263 907 500
PciI A'CATG_T
Cuts at: 3126 3126
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3809 3841 3809
Size: 32 4638
PpuMI rG'GwC_Cy
Cuts at: 920 920
Size: 4670
PspOMI G'GGCC_C
Cuts at: 377 827 1300 377
Size: 450 473 3747
Fragments arranged by size:
3747 473 450
PstI C_TGCA'G
Cuts at: 146 881 1352 1583 1717 2402 146
Size: 735 471 231 134 685 2414
Fragments arranged by size:
2414 735 685 471 231 134
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 2095 2455 2095
Size: 360 4310
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1684 1684
Size: 4670
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 2693 2903 2693
Size: 210 4460
SbfI CC_TGCA'GG
Cuts at: 1352 1717 1352
Size: 365 4305
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SexAI A'CCwGG_T
Cuts at: 1589 1589
Size: 4670
SfcI C'TryA_G
Cuts at: 33 142 455 877 1348 1579 1713 2398 3391
Size: 109 313 422 471 231 134 685 993
Cuts at: 3391 3582 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 471 422 313 231 191
134 109
SfoI GGC'GCC
Cuts at: 329 2102 2350 329
Size: 1773 248 2649
Fragments arranged by size:
2649 1773 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 180 826 1436 1692 180
Size: 646 610 256 3158
Fragments arranged by size:
3158 646 610 256
SmlI C'TyrA_G
Cuts at: 1825 2740 3232 3494 1825
Size: 915 492 262 3001
Fragments arranged by size:
3001 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
SrfI GCCC'GGGC
Cuts at: 1436 1436
Size: 4670
StyI C'CwwG_G
Cuts at: 107 1804 2158 107
Size: 1697 354 2619
Fragments arranged by size:
2619 1697 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 409 2253 2386 2845 3054 409
Size: 1844 133 459 209 2025
Fragments arranged by size:
2025 1844 459 209 133
TatI w'GTAC_w
Cuts at: 4185 4185
Size: 4670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 340 372 793 825 4187 4219 340
Size: 32 421 32 3362 32 791
Fragments arranged by size:
3362 791 421 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2467 2467
Size: 4670
XbaI T'CTAG_A
Cuts at: 1131 1701 1131
Size: 570 4100
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1512 2165 114
Size: 1398 653 2619
Fragments arranged by size:
2619 1398 653
XmaI C'CCGG_G
Cuts at: 178 824 1434 1690 178
Size: 646 610 256 3158
Fragments arranged by size:
3158 646 610 256
ZraI GAC'GTC
Cuts at: 320 320
Size: 4670
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI KpnI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SapI SbfI ScaI SexAI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI ZraI
Enzymes that do not cut:
AfeI AflII AhdI AlfI AloI AscI AvrII BaeI
BbsI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HpaI MfeI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmlI PshAI PsiI
PspXI PsrI SacII SanDI SfiI SgrAI SnaBI SspI
StuI SwaI XhoI XmnI