Restriction map for
pF1KB7794
(Circular) (Six-base) MAPSORT of: pF1KB7794.seq Check: 4659 from: 1 to: 4670
pF1KB7794 4670 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1514 1514
Size: 4670
AccI GT'mk_AC
Cuts at: 1349 1708 1349
Size: 359 4311
Acc65I G'GTAC_C
Cuts at: 1686 1686
Size: 4670
AclI AA'CG_TT
Cuts at: 4510 4510
Size: 4670
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2495 2927 3674 3942 2495
Size: 432 747 268 3223
Fragments arranged by size:
3223 747 432 268
AflIII A'CryG_T
Cuts at: 2022 3126 4103 2022
Size: 1104 977 2589
Fragments arranged by size:
2589 1104 977
AgeI A'CCGG_T
Cuts at: 3058 3058
Size: 4670
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1266 1447 1450 1266
Size: 181 3 4486
Fragments arranged by size:
4486 181 3
AlwNI CAG_nnn'CTG
Cuts at: 1155 1173 1975 3542 1155
Size: 18 802 1567 2283
Fragments arranged by size:
2283 1567 802 18
ApaI G_GGCC'C
Cuts at: 1642 1642
Size: 4670
ApaLI G'TGCA_C
Cuts at: 3440 3440
Size: 4670
ApoI r'AATT_y
Cuts at: 1674 4023 4279 1674
Size: 2349 256 2065
Fragments arranged by size:
2349 2065 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4670
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
AvaI C'yCGr_G
Cuts at: 627 1291 1471 1496 1690 627
Size: 664 180 25 194 3607
Fragments arranged by size:
3607 664 194 180 25
BamHI G'GATC_C
Cuts at: 154 1695 154
Size: 1541 3129
BanI G'GyrC_C
Cuts at: 1585 1686 2100 2348 2383 1585
Size: 101 414 248 35 3872
Fragments arranged by size:
3872 414 248 101 35
BanII G_rGCy'C
Cuts at: 313 403 508 1273 1448 1642 1684 2714 313
Size: 90 105 765 175 194 42 1030 2269
Fragments arranged by size:
2269 1030 765 194 175 105 90 42
BbeI G_GCGC'C
Cuts at: 2104 2352 2104
Size: 248 4422
BbsI GAAGACnn'nnnn_
Cuts at: 1331 1331
Size: 4670
BbvCI CC'TCA_GC
Cuts at: 589 923 589
Size: 334 4336
BciVI GTATCCnnnnn_n'
Cuts at: 2568 3329 4670 2568
Size: 761 1341 2568
Fragments arranged by size:
2568 1341 761
BclI T'GATC_A
Cuts at: 653 1515 2190 653
Size: 862 675 3133
Fragments arranged by size:
3133 862 675
BglI GCCn_nnn'nGGC
Cuts at: 541 565 541
Size: 24 4646
BglII A'GATC_T
Cuts at: 1010 1187 2185 4222 1010
Size: 177 998 2037 1458
Fragments arranged by size:
2037 1458 998 177
BlpI GC'TnA_GC
Cuts at: 1782 1782
Size: 4670
Bme1580I G_kGCm'C
Cuts at: 413 1283 1642 2295 2388 3444 413
Size: 870 359 653 93 1056 1639
Fragments arranged by size:
1639 1056 870 653 359 93
BmrI ACTGGGnnnn_n'
Cuts at: 1232 1882 1963 2298 4442 1232
Size: 650 81 335 2144 1460
Fragments arranged by size:
2144 1460 650 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 273 1317 3037 273
Size: 1044 1720 1906
Fragments arranged by size:
1906 1720 1044
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 774 1132 1144 1150 1176 691
Size: 83 358 12 6 26 4185
Fragments arranged by size:
4185 358 83 26 12 6
Bpu10I CC'TnA_GC
Cuts at: 218 376 589 923 218
Size: 158 213 334 3965
Fragments arranged by size:
3965 334 213 158
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1846 2725 3217 3515 1846
Size: 879 492 298 3001
Fragments arranged by size:
3001 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1111 1531 4374 1111
Size: 420 2843 1407
Fragments arranged by size:
2843 1407 420
BsaAI yAC'GTr
Cuts at: 2023 2023
Size: 4670
BsaBI GATnn'nnATC
Cuts at: 6 2184 4221 6
Size: 2178 2037 455
Fragments arranged by size:
2178 2037 455
BsaHI Gr'CG_yC
Cuts at: 113 727 1346 2101 2349 3051 4547 113
Size: 614 619 755 248 702 1496 236
Fragments arranged by size:
1496 755 702 619 614 248 236
BsaWI w'CCGG_w
Cuts at: 699 989 1859 2380 3058 3332 3479 3890 699
Size: 290 870 521 678 274 147 411 1479
Fragments arranged by size:
1479 870 678 521 411 290 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 355 385 1174 1204 355
Size: 30 789 30 3821
Fragments arranged by size:
3821 789 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 807 835 913 916 982 1290 807
Size: 28 78 3 66 308 4187
Fragments arranged by size:
4187 308 78 66 28 3
BseYI C'CCAG_C
Cuts at: 462 582 733 1266 1457 2095 2853 3430 3926
Size: 120 151 533 191 638 758 577 496
Cuts at: 3926 462
Size: 1206
Fragments arranged by size:
1206 758 638 577 533 496 191 151
120
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 733
Size: 4670
BsiEI CG_ry'CG
Cuts at: 104 137 560 2212 2258 3466 104
Size: 33 423 1652 46 1208 1308
Fragments arranged by size:
1652 1308 1208 423 46 33
BsiHKAI G_wGCw'C
Cuts at: 224 365 481 1684 2462 2652 3444 224
Size: 141 116 1203 778 190 792 1450
Fragments arranged by size:
1450 1203 792 778 190 141 116
BsmI GAATG_Cn'
Cuts at: 1134 1134
Size: 4670
Bsp1286I G_dGCh'C
Cuts at: 224 313 365 403 413 481 508 1273 1283
Size: 89 52 38 10 68 27 765 10
Cuts at: 1283 1448 1642 1684 2295 2388 2462 2652 2714
Size: 165 194 42 611 93 74 190 62
Cuts at: 2714 3444 224
Size: 730 1450
Fragments arranged by size:
1450 765 730 611 194 190 165 93
89 74 68 62 52 42 38 27
10 10
BspEI T'CCGG_A
Cuts at: 989 3890 989
Size: 2901 1769
BspHI T'CATG_A
Cuts at: 3846 3846
Size: 4670
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1237 1514 1720 1934 2236 2617 1237
Size: 277 206 214 302 381 3290
Fragments arranged by size:
3290 381 302 277 214 206
BsrBI CCG'CTC
Cuts at: 50 380 776 2962 3016 4500 4665 50
Size: 330 396 2186 54 1484 165 55
Fragments arranged by size:
2186 1484 396 330 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2582 2582
Size: 4670
BsrFI r'CCGG_y
Cuts at: 1479 2668 2849 3058 1479
Size: 1189 181 209 3091
Fragments arranged by size:
3091 1189 209 181
BssHII G'CGCG_C
Cuts at: 811 811
Size: 4670
BssSI C'ACGA_G
Cuts at: 1276 1534 2941 3299 1276
Size: 258 1407 358 2647
Fragments arranged by size:
2647 1407 358 258
BstAPI GCAn_nnn'nTGC
Cuts at: 2047 2047
Size: 4670
BstBI TT'CG_AA
Cuts at: 3031 3031
Size: 4670
BstEII G'GTnAC_C
Cuts at: 1176 1176
Size: 4670
BstXI CCAn_nnnn'nTGG
Cuts at: 165 420 1457 165
Size: 255 1037 3378
Fragments arranged by size:
3378 1037 255
BstYI r'GATC_y
Cuts at: 1 154 177 1010 1187 1632 1695 2162 2185
Size: 153 23 833 177 445 63 467 23
Cuts at: 2185 2520 2766 3778 3864 3876 4222 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 833 467 449 445 346 335 246
177 153 86 63 23 23 12
BtgI C'CryG_G
Cuts at: 107 518 545 554 563 741 806 821 1439
Size: 411 27 9 9 178 65 15 618
Cuts at: 1439 107
Size: 3338
Fragments arranged by size:
3338 618 411 178 65 27 15 9
9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2620 2801 2964 2620
Size: 181 163 4326
Fragments arranged by size:
4326 181 163
BtsI GCAGTG_nn'
Cuts at: 753 848 883 1913 753
Size: 95 35 1030 3510
Fragments arranged by size:
3510 1030 95 35
DraI TTT'AAA
Cuts at: 1669 1669
Size: 4670
DrdI GACnn_nn'nnGTC
Cuts at: 2376 3234 2376
Size: 858 3812
EaeI y'GGCC_r
Cuts at: 134 542 557 566 824 1208 1334 1355 1419
Size: 408 15 9 258 384 126 21 64
Cuts at: 1419 2255 2429 2820 2847 4192 134
Size: 836 174 391 27 1345 612
Fragments arranged by size:
1345 836 612 408 391 384 258 174
126 64 27 21 15 9
EagI C'GGCC_G
Cuts at: 134 557 2255 134
Size: 423 1698 2549
Fragments arranged by size:
2549 1698 423
EarI CTCTTCn'nnn_
Cuts at: 301 976 2693 2903 301
Size: 675 1717 210 2068
Fragments arranged by size:
2068 1717 675 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 373 2878 3188 3334 4480 373
Size: 2505 310 146 1146 563
Fragments arranged by size:
2505 1146 563 310 146
EcoICRI GAG'CTC
Cuts at: 1682 1682
Size: 4670
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 691 774 1132 1144 1150 1176 2495 2927 3674
Size: 83 358 12 6 26 1319 432 747
Cuts at: 3674 3942 691
Size: 268 1419
Fragments arranged by size:
1419 1319 747 432 358 268 83 26
12 6
EcoNI CCTnn'n_nnAGG
Cuts at: 344 600 895 1555 344
Size: 256 295 660 3459
Fragments arranged by size:
3459 660 295 256
EcoO109I rG'GnC_Cy
Cuts at: 624 705 1139 1226 1639 1809 624
Size: 81 434 87 413 170 3485
Fragments arranged by size:
3485 434 413 170 87 81
EcoRI G'AATT_C
Cuts at: 1674 1674
Size: 4670
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2689 2689
Size: 4670
FspI TGC'GCA
Cuts at: 209 2451 209
Size: 2242 2428
FspAI rTGC'GCAy
Cuts at: 209 209
Size: 4670
HaeII r_GCGC'y
Cuts at: 394 463 2104 2352 3374 4353 394
Size: 69 1641 248 1022 979 711
Fragments arranged by size:
1641 1022 979 711 248 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2512 2542 2544 2574 2512
Size: 30 2 30 4608
Fragments arranged by size:
4608 30 30 2
HincII GTy'rAC
Cuts at: 1709 1709
Size: 4670
KasI G'GCGC_C
Cuts at: 2100 2348 2100
Size: 248 4422
KpnI G_GTAC'C
Cuts at: 1690 1690
Size: 4670
MluI A'CGCG_T
Cuts at: 4103 4103
Size: 4670
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 170 748 3341 3525 170
Size: 578 2593 184 1315
Fragments arranged by size:
2593 1315 578 184
MscI TGG'CCA
Cuts at: 568 2431 568
Size: 1863 2807
MslI CAynn'nnrTG
Cuts at: 418 487 746 2659 2786 418
Size: 69 259 1913 127 2302
Fragments arranged by size:
2302 1913 259 127 69
MspA1I CmG'CkG
Cuts at: 556 579 645 1155 1491 1781 2095 2455 3468
Size: 23 66 510 336 290 314 360 1013
Cuts at: 3468 3713 556
Size: 245 1513
Fragments arranged by size:
1513 1013 510 360 336 314 290 245
66 23
NaeI GCC'GGC
Cuts at: 1481 2851 1481
Size: 1370 3300
NarI GG'CG_CC
Cuts at: 2101 2349 2101
Size: 248 4422
NcoI C'CATG_G
Cuts at: 107 545 563 741 806 1439 107
Size: 438 18 178 65 633 3338
Fragments arranged by size:
3338 633 438 178 65 18
NgoMIV G'CCGG_C
Cuts at: 1479 2849 1479
Size: 1370 3300
NotI GC'GGCC_GC
Cuts at: 557 557
Size: 4670
NspI r_CATG'y
Cuts at: 1723 2223 3130 1723
Size: 500 907 3263
Fragments arranged by size:
3263 907 500
PciI A'CATG_T
Cuts at: 3126 3126
Size: 4670
PflMI CCAn_nnn'nTGG
Cuts at: 1173 1616 1173
Size: 443 4227
PfoI T'CCnGG_A
Cuts at: 164 164
Size: 4670
PmeI GTTT'AAAC
Cuts at: 1669 1669
Size: 4670
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 997 1029 3809 3841 997
Size: 32 2780 32 1826
Fragments arranged by size:
2780 1826 32 32
PpuMI rG'GwC_Cy
Cuts at: 705 1139 1226 705
Size: 434 87 4149
Fragments arranged by size:
4149 434 87
PshAI GACnn'nnGTC
Cuts at: 1174 1174
Size: 4670
PspOMI G'GGCC_C
Cuts at: 1638 1638
Size: 4670
PstI C_TGCA'G
Cuts at: 1160 1511 1717 2402 1160
Size: 351 206 685 3428
Fragments arranged by size:
3428 685 351 206
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4670
PvuII CAG'CTG
Cuts at: 645 1155 2095 2455 645
Size: 510 940 360 2860
Fragments arranged by size:
2860 940 510 360
RsrII CG'GwC_CG
Cuts at: 2865 2865
Size: 4670
SacI G_AGCT'C
Cuts at: 1684 1684
Size: 4670
SacII CC_GC'GG
Cuts at: 557 557
Size: 4670
SalI G'TCGA_C
Cuts at: 1707 1707
Size: 4670
SapI GCTCTTCn'nnn_
Cuts at: 301 2693 2903 301
Size: 2392 210 2068
Fragments arranged by size:
2392 2068 210
SbfI CC_TGCA'GG
Cuts at: 1717 1717
Size: 4670
ScaI AGT'ACT
Cuts at: 4187 4187
Size: 4670
SexAI A'CCwGG_T
Cuts at: 707 707
Size: 4670
SfcI C'TryA_G
Cuts at: 33 1156 1507 1713 2398 3391 3582 33
Size: 1123 351 206 685 993 191 1121
Fragments arranged by size:
1123 1121 993 685 351 206 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 565 565
Size: 4670
SfoI GGC'GCC
Cuts at: 2102 2350 2102
Size: 248 4422
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4670
SmaI CCC'GGG
Cuts at: 629 1473 1498 1692 629
Size: 844 25 194 3607
Fragments arranged by size:
3607 844 194 25
SmlI C'TyrA_G
Cuts at: 1825 2740 3232 3494 1825
Size: 915 492 262 3001
Fragments arranged by size:
3001 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4670
SphI G_CATG'C
Cuts at: 1723 2223 1723
Size: 500 4170
StuI AGG'CCT
Cuts at: 976 1561 976
Size: 585 4085
StyI C'CwwG_G
Cuts at: 107 545 563 741 806 1439 1610 1804 2158
Size: 438 18 178 65 633 171 194 354
Cuts at: 2158 107
Size: 2619
Fragments arranged by size:
2619 633 438 354 194 178 171 65
18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 448 1231 1546 2253 2386 2845 3054 448
Size: 783 315 707 133 459 209 2064
Fragments arranged by size:
2064 783 707 459 315 209 133
TatI w'GTAC_w
Cuts at: 4185 4185
Size: 4670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 355 387 4187 4219 355
Size: 32 3800 32 806
Fragments arranged by size:
3800 806 32 32
Tth111I GACn'n_nGTC
Cuts at: 115 1345 2467 115
Size: 1230 1122 2318
Fragments arranged by size:
2318 1230 1122
XbaI T'CTAG_A
Cuts at: 1059 1701 1059
Size: 642 4028
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 579 2165 579
Size: 1586 3084
XmaI C'CCGG_G
Cuts at: 627 1471 1496 1690 627
Size: 844 25 194 3607
Fragments arranged by size:
3607 844 194 25
XmnI GAAnn'nnTTC
Cuts at: 682 682
Size: 4670
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BbvCI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NotI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PshAI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BcgI BfrBI BmgBI BmtI BsiWI BsmBI BsrGI
BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI HindIII
HpaI MfeI NdeI NheI NruI NsiI PacI PasI
PmlI PsiI PspXI PsrI SanDI SgrAI SnaBI SrfI
SspI SwaI XhoI ZraI