Restriction map for
pF1KB7789
(Circular) (Six-base) MAPSORT of: pF1KB7789.seq Check: 5642 from: 1 to: 4487
pF1KB7789 4487 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 419 1525 419
Size: 1106 3381
Acc65I G'GTAC_C
Cuts at: 1503 1503
Size: 4487
AclI AA'CG_TT
Cuts at: 4327 4327
Size: 4487
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 347 2312 2744 3491 3759 134
Size: 213 1965 432 747 268 862
Fragments arranged by size:
1965 862 747 432 268 213
AflIII A'CryG_T
Cuts at: 1839 2943 3920 1839
Size: 1104 977 2406
Fragments arranged by size:
2406 1104 977
AgeI A'CCGG_T
Cuts at: 2875 2875
Size: 4487
AleI CACnn'nnGTG
Cuts at: 631 631
Size: 4487
AlwNI CAG_nnn'CTG
Cuts at: 894 1199 1792 3359 894
Size: 305 593 1567 2022
Fragments arranged by size:
2022 1567 593 305
ApaI G_GGCC'C
Cuts at: 182 996 182
Size: 814 3673
ApaLI G'TGCA_C
Cuts at: 3257 3257
Size: 4487
ApoI r'AATT_y
Cuts at: 370 1491 3840 4096 370
Size: 1121 2349 256 761
Fragments arranged by size:
2349 1121 761 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4487
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
AvaI C'yCGr_G
Cuts at: 175 181 219 341 814 1507 175
Size: 6 38 122 473 693 3155
Fragments arranged by size:
3155 693 473 122 38 6
BamHI G'GATC_C
Cuts at: 1512 1512
Size: 4487
BanI G'GyrC_C
Cuts at: 1443 1503 1917 2165 2200 1443
Size: 60 414 248 35 3730
Fragments arranged by size:
3730 414 248 60 35
BanII G_rGCy'C
Cuts at: 176 182 497 996 1124 1501 2531 176
Size: 6 315 499 128 377 1030 2132
Fragments arranged by size:
2132 1030 499 377 315 128 6
BbeI G_GCGC'C
Cuts at: 1921 2169 1921
Size: 248 4239
BbsI GAAGACnn'nnnn_
Cuts at: 574 710 574
Size: 136 4351
BbvCI CC'TCA_GC
Cuts at: 200 1261 200
Size: 1061 3426
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 658 692 658
Size: 34 4453
BciVI GTATCCnnnnn_n'
Cuts at: 2385 3146 4487 2385
Size: 761 1341 2385
Fragments arranged by size:
2385 1341 761
BclI T'GATC_A
Cuts at: 2007 2007
Size: 4487
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4487
BglII A'GATC_T
Cuts at: 824 2002 4039 824
Size: 1178 2037 1272
Fragments arranged by size:
2037 1272 1178
BlpI GC'TnA_GC
Cuts at: 1183 1599 1183
Size: 416 4071
Bme1580I G_kGCm'C
Cuts at: 182 996 1243 1395 1446 2112 2205 3261 182
Size: 814 247 152 51 666 93 1056 1408
Fragments arranged by size:
1408 1056 814 666 247 152 93 51
BmgBI CAC'GTC
Cuts at: 945 945
Size: 4487
BmrI ACTGGGnnnn_n'
Cuts at: 1389 1699 1780 2115 4259 1389
Size: 310 81 335 2144 1617
Fragments arranged by size:
2144 1617 335 310 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2854 2854
Size: 4487
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1153 1237 1153
Size: 84 4403
Bpu10I CC'TnA_GC
Cuts at: 200 487 509 1261 200
Size: 287 22 752 3426
Fragments arranged by size:
3426 752 287 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 733 1420 1663 2542 3034 3332 733
Size: 687 243 879 492 298 1888
Fragments arranged by size:
1888 879 687 492 298 243
BsaI GGTCTCn'nnnn_
Cuts at: 4191 4191
Size: 4487
BsaAI yAC'GTr
Cuts at: 387 1347 1840 387
Size: 960 493 3034
Fragments arranged by size:
3034 960 493
BsaBI GATnn'nnATC
Cuts at: 6 655 2001 4038 6
Size: 649 1346 2037 455
Fragments arranged by size:
2037 1346 649 455
BsaHI Gr'CG_yC
Cuts at: 1918 2166 2868 4364 1918
Size: 248 702 1496 2041
Fragments arranged by size:
2041 1496 702 248
BsaWI w'CCGG_w
Cuts at: 303 1676 2197 2875 3149 3296 3707 303
Size: 1373 521 678 274 147 411 1083
Fragments arranged by size:
1373 1083 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1012 1042 1012
Size: 30 4457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1390 1390
Size: 4487
BseYI C'CCAG_C
Cuts at: 1912 2670 3247 3743 1912
Size: 758 577 496 2656
Fragments arranged by size:
2656 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 583 583
Size: 4487
BsiEI CG_ry'CG
Cuts at: 104 197 304 2029 2075 3283 104
Size: 93 107 1725 46 1208 1308
Fragments arranged by size:
1725 1308 1208 107 93 46
BsiHKAI G_wGCw'C
Cuts at: 497 1124 1141 1382 1501 2279 2469 3261 497
Size: 627 17 241 119 778 190 792 1723
Fragments arranged by size:
1723 792 778 627 241 190 119 17
BsmI GAATG_Cn'
Cuts at: 195 318 195
Size: 123 4364
BsmBI CGTCTCn'nnnn_
Cuts at: 234 234
Size: 4487
Bsp1286I G_dGCh'C
Cuts at: 176 182 497 996 1124 1141 1243 1382 1395
Size: 6 315 499 128 17 102 139 13
Cuts at: 1395 1446 1501 2112 2205 2279 2469 2531 3261
Size: 51 55 611 93 74 190 62 730
Cuts at: 3261 176
Size: 1402
Fragments arranged by size:
1402 730 611 499 315 190 139 128
102 93 74 62 55 51 17 13
6
BspEI T'CCGG_A
Cuts at: 3707 3707
Size: 4487
BspHI T'CATG_A
Cuts at: 3663 3663
Size: 4487
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1537 1751 2053 2434 1537
Size: 214 302 381 3590
Fragments arranged by size:
3590 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2779 2833 4317 4482 50
Size: 2729 54 1484 165 55
Fragments arranged by size:
2729 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2399 2399
Size: 4487
BsrFI r'CCGG_y
Cuts at: 296 398 428 983 2485 2666 2875 296
Size: 102 30 555 1502 181 209 1908
Fragments arranged by size:
1908 1502 555 209 181 102 30
BsrGI T'GTAC_A
Cuts at: 378 462 378
Size: 84 4403
BssHII G'CGCG_C
Cuts at: 247 247
Size: 4487
BssSI C'ACGA_G
Cuts at: 2758 3116 2758
Size: 358 4129
BstAPI GCAn_nnn'nTGC
Cuts at: 1864 1864
Size: 4487
BstBI TT'CG_AA
Cuts at: 2848 2848
Size: 4487
BstXI CCAn_nnnn'nTGG
Cuts at: 1003 1371 1419 1003
Size: 368 48 4071
Fragments arranged by size:
4071 368 48
BstYI r'GATC_y
Cuts at: 1 650 824 1220 1512 1979 2002 2337 2583
Size: 649 174 396 292 467 23 335 246
Cuts at: 2583 3595 3681 3693 4039 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 649 467 449 396 346 335 292
246 174 86 23 12
BtgI C'CryG_G
Cuts at: 107 1083 107
Size: 976 3511
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1089 2437 2618 2781 1089
Size: 1348 181 163 2795
Fragments arranged by size:
2795 1348 181 163
BtsI GCAGTG_nn'
Cuts at: 1730 1730
Size: 4487
DraI TTT'AAA
Cuts at: 1486 1486
Size: 4487
DraIII CAC_nnn'GTG
Cuts at: 387 387
Size: 4487
DrdI GACnn_nn'nnGTC
Cuts at: 2193 3051 2193
Size: 858 3629
EaeI y'GGCC_r
Cuts at: 194 905 2072 2246 2637 2664 4009 194
Size: 711 1167 174 391 27 1345 672
Fragments arranged by size:
1345 1167 711 672 391 174 27
EagI C'GGCC_G
Cuts at: 194 2072 194
Size: 1878 2609
EarI CTCTTCn'nnn_
Cuts at: 763 2510 2720 763
Size: 1747 210 2530
Fragments arranged by size:
2530 1747 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 224 2695 3005 3151 4297 224
Size: 2471 310 146 1146 414
Fragments arranged by size:
2471 1146 414 310 146
EcoICRI GAG'CTC
Cuts at: 495 1122 1499 495
Size: 627 377 3483
Fragments arranged by size:
3483 627 377
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 134 347 1153 1237 2312 2744 3491 3759 134
Size: 213 806 84 1075 432 747 268 862
Fragments arranged by size:
1075 862 806 747 432 268 213 84
EcoNI CCTnn'n_nnAGG
Cuts at: 973 973
Size: 4487
EcoO109I rG'GnC_Cy
Cuts at: 992 1257 1626 992
Size: 265 369 3853
Fragments arranged by size:
3853 369 265
EcoRI G'AATT_C
Cuts at: 370 1491 370
Size: 1121 3366
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2506 2506
Size: 4487
FspI TGC'GCA
Cuts at: 2268 2268
Size: 4487
HaeII r_GCGC'y
Cuts at: 439 1921 2169 3191 4170 439
Size: 1482 248 1022 979 756
Fragments arranged by size:
1482 1022 979 756 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 324 356 870 902 1012 1044 2329 2359 2361
Size: 32 514 32 110 32 1285 30 2
Cuts at: 2361 2391 324
Size: 30 2420
Fragments arranged by size:
2420 1285 514 110 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1526 1526
Size: 4487
HindIII A'AGCT_T
Cuts at: 910 910
Size: 4487
KasI G'GCGC_C
Cuts at: 1917 2165 1917
Size: 248 4239
KpnI G_GTAC'C
Cuts at: 1507 1507
Size: 4487
MluI A'CGCG_T
Cuts at: 3920 3920
Size: 4487
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3158 3342 3158
Size: 184 4303
MscI TGG'CCA
Cuts at: 907 2248 907
Size: 1341 3146
MslI CAynn'nnrTG
Cuts at: 631 847 1417 2476 2603 631
Size: 216 570 1059 127 2515
Fragments arranged by size:
2515 1059 570 216 127
MspA1I CmG'CkG
Cuts at: 199 1085 1164 1598 1912 2272 3285 3530 199
Size: 886 79 434 314 360 1013 245 1156
Fragments arranged by size:
1156 1013 886 434 360 314 245 79
NaeI GCC'GGC
Cuts at: 298 2668 298
Size: 2370 2117
NarI GG'CG_CC
Cuts at: 1918 2166 1918
Size: 248 4239
NcoI C'CATG_G
Cuts at: 107 107
Size: 4487
NgoMIV G'CCGG_C
Cuts at: 296 2666 296
Size: 2370 2117
NotI GC'GGCC_GC
Cuts at: 194 194
Size: 4487
NspI r_CATG'y
Cuts at: 1540 2040 2947 1540
Size: 500 907 3080
Fragments arranged by size:
3080 907 500
PciI A'CATG_T
Cuts at: 2943 2943
Size: 4487
PflMI CCAn_nnn'nTGG
Cuts at: 728 728
Size: 4487
PfoI T'CCnGG_A
Cuts at: 917 917
Size: 4487
PmeI GTTT'AAAC
Cuts at: 1486 1486
Size: 4487
PmlI CAC'GTG
Cuts at: 1347 1347
Size: 4487
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3626 3658 3626
Size: 32 4455
PpuMI rG'GwC_Cy
Cuts at: 1257 1257
Size: 4487
PspOMI G'GGCC_C
Cuts at: 178 992 178
Size: 814 3673
PstI C_TGCA'G
Cuts at: 1534 2219 1534
Size: 685 3802
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4487
PvuII CAG'CTG
Cuts at: 1912 2272 1912
Size: 360 4127
RsrII CG'GwC_CG
Cuts at: 2682 2682
Size: 4487
SacI G_AGCT'C
Cuts at: 497 1124 1501 497
Size: 627 377 3483
Fragments arranged by size:
3483 627 377
SacII CC_GC'GG
Cuts at: 1086 1086
Size: 4487
SalI G'TCGA_C
Cuts at: 1524 1524
Size: 4487
SapI GCTCTTCn'nnn_
Cuts at: 763 2510 2720 763
Size: 1747 210 2530
Fragments arranged by size:
2530 1747 210
SbfI CC_TGCA'GG
Cuts at: 1534 1534
Size: 4487
ScaI AGT'ACT
Cuts at: 742 1330 4004 742
Size: 588 2674 1225
Fragments arranged by size:
2674 1225 588
SfcI C'TryA_G
Cuts at: 33 1530 2215 3208 3399 33
Size: 1497 685 993 191 1121
Fragments arranged by size:
1497 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1919 2167 1919
Size: 248 4239
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4487
SmaI CCC'GGG
Cuts at: 177 183 221 816 1509 177
Size: 6 38 595 693 3155
Fragments arranged by size:
3155 693 595 38 6
SmlI C'TyrA_G
Cuts at: 341 748 1435 1642 2557 3049 3311 341
Size: 407 687 207 915 492 262 1517
Fragments arranged by size:
1517 915 687 492 407 262 207
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4487
SphI G_CATG'C
Cuts at: 1540 2040 1540
Size: 500 3987
SrfI GCCC'GGGC
Cuts at: 177 177
Size: 4487
StyI C'CwwG_G
Cuts at: 107 602 629 1016 1621 1975 107
Size: 495 27 387 605 354 2619
Fragments arranged by size:
2619 605 495 387 354 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1197 1297 1377 1425 2070 2203 2662 2871 1197
Size: 100 80 48 645 133 459 209 2813
Fragments arranged by size:
2813 645 459 209 133 100 80 48
TatI w'GTAC_w
Cuts at: 378 462 740 752 935 1328 4002 378
Size: 84 278 12 183 393 2674 863
Fragments arranged by size:
2674 863 393 278 183 84 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1031 1063 4004 4036 1031
Size: 32 2941 32 1482
Fragments arranged by size:
2941 1482 32 32
Tth111I GACn'n_nGTC
Cuts at: 1426 2284 1426
Size: 858 3629
XbaI T'CTAG_A
Cuts at: 1518 1518
Size: 4487
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 600 915 1982 600
Size: 315 1067 3105
Fragments arranged by size:
3105 1067 315
XhoI C'TCGA_G
Cuts at: 341 341
Size: 4487
XmaI C'CCGG_G
Cuts at: 175 181 219 814 1507 175
Size: 6 38 595 693 3155
Fragments arranged by size:
3155 693 595 38 6
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NotI NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SacII SalI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SrfI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BfrBI BmtI BsiWI BstEII BstZ17I Bsu36I
ClaI CspCI EcoRV FseI FspAI HpaI MfeI NdeI
NheI NruI NsiI PacI PasI PshAI PsiI PspXI
PsrI SanDI SexAI SfiI SgrAI SnaBI SspI StuI
SwaI XmnI ZraI