Restriction map for
pF1KB7788
(Circular) (Six-base) MAPSORT of: pF1KB7788.seq Check: 1384 from: 1 to: 4655
pF1KB7788 4655 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1110 1693 1110
Size: 583 4072
Acc65I G'GTAC_C
Cuts at: 1671 1671
Size: 4655
AclI AA'CG_TT
Cuts at: 1593 4495 1593
Size: 2902 1753
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 848 961 1601 2480 2912 3659 3927 277
Size: 571 113 640 879 432 747 268 1005
Fragments arranged by size:
1005 879 747 640 571 432 268 113
AflIII A'CryG_T
Cuts at: 1647 2007 3111 4088 1647
Size: 360 1104 977 2214
Fragments arranged by size:
2214 1104 977 360
AgeI A'CCGG_T
Cuts at: 3043 3043
Size: 4655
AleI CACnn'nnGTG
Cuts at: 768 768
Size: 4655
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 584 616 584
Size: 32 4623
AlwNI CAG_nnn'CTG
Cuts at: 667 1381 1960 3527 667
Size: 714 579 1567 1795
Fragments arranged by size:
1795 1567 714 579
ApaLI G'TGCA_C
Cuts at: 3425 3425
Size: 4655
ApoI r'AATT_y
Cuts at: 225 1659 4008 4264 225
Size: 1434 2349 256 616
Fragments arranged by size:
2349 1434 616 256
AseI AT'TA_AT
Cuts at: 20 1136 20
Size: 1116 3539
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
AvaI C'yCGr_G
Cuts at: 1675 1675
Size: 4655
BamHI G'GATC_C
Cuts at: 1680 1680
Size: 4655
BanI G'GyrC_C
Cuts at: 1671 2085 2333 2368 1671
Size: 414 248 35 3958
Fragments arranged by size:
3958 414 248 35
BanII G_rGCy'C
Cuts at: 1669 2699 1669
Size: 1030 3625
BbeI G_GCGC'C
Cuts at: 2089 2337 2089
Size: 248 4407
BbsI GAAGACnn'nnnn_
Cuts at: 130 130
Size: 4655
BciVI GTATCCnnnnn_n'
Cuts at: 1484 2553 3314 4655 1484
Size: 1069 761 1341 1484
Fragments arranged by size:
1484 1341 1069 761
BclI T'GATC_A
Cuts at: 2175 2175
Size: 4655
BglII A'GATC_T
Cuts at: 849 1285 2170 4207 849
Size: 436 885 2037 1297
Fragments arranged by size:
2037 1297 885 436
BlpI GC'TnA_GC
Cuts at: 1767 1767
Size: 4655
Bme1580I G_kGCm'C
Cuts at: 2280 2373 3429 2280
Size: 93 1056 3506
Fragments arranged by size:
3506 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1867 1948 2283 4427 1867
Size: 81 335 2144 2095
Fragments arranged by size:
2144 2095 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3022 3022
Size: 4655
Bpu10I CC'TnA_GC
Cuts at: 1374 1374
Size: 4655
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1831 2710 3202 3500 1831
Size: 879 492 298 2986
Fragments arranged by size:
2986 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4359 4359
Size: 4655
BsaAI yAC'GTr
Cuts at: 2008 2008
Size: 4655
BsaBI GATnn'nnATC
Cuts at: 6 2169 4206 6
Size: 2163 2037 455
Fragments arranged by size:
2163 2037 455
BsaHI Gr'CG_yC
Cuts at: 2086 2334 3036 4532 2086
Size: 248 702 1496 2209
Fragments arranged by size:
2209 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1844 2365 3043 3317 3464 3875 1844
Size: 521 678 274 147 411 2624
Fragments arranged by size:
2624 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 140 170 140
Size: 30 4625
BseYI C'CCAG_C
Cuts at: 2080 2838 3415 3911 2080
Size: 758 577 496 2824
Fragments arranged by size:
2824 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2197 2243 3451 104
Size: 2093 46 1208 1308
Fragments arranged by size:
2093 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 493 1669 2447 2637 3429 493
Size: 1176 778 190 792 1719
Fragments arranged by size:
1719 1176 792 778 190
BsmI GAATG_Cn'
Cuts at: 863 1073 1428 863
Size: 210 355 4090
Fragments arranged by size:
4090 355 210
Bsp1286I G_dGCh'C
Cuts at: 493 1669 2280 2373 2447 2637 2699 3429 493
Size: 1176 611 93 74 190 62 730 1719
Fragments arranged by size:
1719 1176 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3875 3875
Size: 4655
BspHI T'CATG_A
Cuts at: 3831 3831
Size: 4655
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1705 1919 2221 2602 1705
Size: 214 302 381 3758
Fragments arranged by size:
3758 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2947 3001 4485 4650 50
Size: 2897 54 1484 165 55
Fragments arranged by size:
2897 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2567 2567
Size: 4655
BsrFI r'CCGG_y
Cuts at: 2653 2834 3043 2653
Size: 181 209 4265
Fragments arranged by size:
4265 209 181
BsrGI T'GTAC_A
Cuts at: 516 1553 516
Size: 1037 3618
BssSI C'ACGA_G
Cuts at: 2926 3284 2926
Size: 358 4297
BstAPI GCAn_nnn'nTGC
Cuts at: 677 2032 677
Size: 1355 3300
BstBI TT'CG_AA
Cuts at: 583 3016 583
Size: 2433 2222
BstYI r'GATC_y
Cuts at: 1 849 1285 1680 2147 2170 2505 2751 3763
Size: 848 436 395 467 23 335 246 1012
Cuts at: 3763 3849 3861 4207 1
Size: 86 12 346 449
Fragments arranged by size:
1012 848 467 449 436 395 346 335
246 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 183 183
Size: 4655
BtgI C'CryG_G
Cuts at: 107 107
Size: 4655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 1115 2605 2786 2949 1115
Size: 1490 181 163 2821
Fragments arranged by size:
2821 1490 181 163
BtsI GCAGTG_nn'
Cuts at: 220 493 1898 220
Size: 273 1405 2977
Fragments arranged by size:
2977 1405 273
DraI TTT'AAA
Cuts at: 1170 1598 1654 1170
Size: 428 56 4171
Fragments arranged by size:
4171 428 56
DrdI GACnn_nn'nnGTC
Cuts at: 1268 2361 3219 1268
Size: 1093 858 2704
Fragments arranged by size:
2704 1093 858
EaeI y'GGCC_r
Cuts at: 2240 2414 2805 2832 4177 2240
Size: 174 391 27 1345 2718
Fragments arranged by size:
2718 1345 391 174 27
EagI C'GGCC_G
Cuts at: 2240 2240
Size: 4655
EarI CTCTTCn'nnn_
Cuts at: 580 582 1338 1535 1609 2678 2888 580
Size: 2 756 197 74 1069 210 2347
Fragments arranged by size:
2347 1069 756 210 197 74 2
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2863 3173 3319 4465 2863
Size: 310 146 1146 3053
Fragments arranged by size:
3053 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1667 1667
Size: 4655
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 277 848 961 1601 2480 2912 3659 3927 277
Size: 571 113 640 879 432 747 268 1005
Fragments arranged by size:
1005 879 747 640 571 432 268 113
EcoO109I rG'GnC_Cy
Cuts at: 1261 1794 1261
Size: 533 4122
EcoRI G'AATT_C
Cuts at: 1659 1659
Size: 4655
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 392 2674 392
Size: 2282 2373
FspI TGC'GCA
Cuts at: 2436 2436
Size: 4655
HaeII r_GCGC'y
Cuts at: 2089 2337 3359 4338 2089
Size: 248 1022 979 2406
Fragments arranged by size:
2406 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 649 681 939 971 2497 2527 2529 2559 649
Size: 32 258 32 1526 30 2 30 2745
Fragments arranged by size:
2745 1526 258 32 32 30 30 2
HincII GTy'rAC
Cuts at: 947 1694 947
Size: 747 3908
HindIII A'AGCT_T
Cuts at: 1050 1050
Size: 4655
KasI G'GCGC_C
Cuts at: 2085 2333 2085
Size: 248 4407
KpnI G_GTAC'C
Cuts at: 1675 1675
Size: 4655
MluI A'CGCG_T
Cuts at: 4088 4088
Size: 4655
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1591 3326 3510 1591
Size: 1735 184 2736
Fragments arranged by size:
2736 1735 184
MscI TGG'CCA
Cuts at: 2416 2416
Size: 4655
MslI CAynn'nnrTG
Cuts at: 551 768 1127 1189 1501 2644 2771 551
Size: 217 359 62 312 1143 127 2435
Fragments arranged by size:
2435 1143 359 312 217 127 62
MspA1I CmG'CkG
Cuts at: 336 1766 2080 2440 3453 3698 336
Size: 1430 314 360 1013 245 1293
Fragments arranged by size:
1430 1293 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2836 2836
Size: 4655
NarI GG'CG_CC
Cuts at: 2086 2334 2086
Size: 248 4407
NcoI C'CATG_G
Cuts at: 107 107
Size: 4655
NgoMIV G'CCGG_C
Cuts at: 2834 2834
Size: 4655
NruI TCG'CGA
Cuts at: 460 460
Size: 4655
NspI r_CATG'y
Cuts at: 330 684 1651 1708 2208 3115 330
Size: 354 967 57 500 907 1870
Fragments arranged by size:
1870 967 907 500 354 57
PciI A'CATG_T
Cuts at: 1647 3111 1647
Size: 1464 3191
PflMI CCAn_nnn'nTGG
Cuts at: 113 113
Size: 4655
PfoI T'CCnGG_A
Cuts at: 1116 1116
Size: 4655
PmeI GTTT'AAAC
Cuts at: 1654 1654
Size: 4655
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3794 3826 3794
Size: 32 4623
PpuMI rG'GwC_Cy
Cuts at: 1261 1261
Size: 4655
PstI C_TGCA'G
Cuts at: 215 1206 1702 2387 215
Size: 991 496 685 2483
Fragments arranged by size:
2483 991 685 496
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4655
PvuII CAG'CTG
Cuts at: 336 2080 2440 336
Size: 1744 360 2551
Fragments arranged by size:
2551 1744 360
RsrII CG'GwC_CG
Cuts at: 2850 2850
Size: 4655
SacI G_AGCT'C
Cuts at: 1669 1669
Size: 4655
SalI G'TCGA_C
Cuts at: 1692 1692
Size: 4655
SapI GCTCTTCn'nnn_
Cuts at: 2678 2888 2678
Size: 210 4445
SbfI CC_TGCA'GG
Cuts at: 1702 1702
Size: 4655
ScaI AGT'ACT
Cuts at: 4172 4172
Size: 4655
SexAI A'CCwGG_T
Cuts at: 1263 1263
Size: 4655
SfcI C'TryA_G
Cuts at: 33 211 1202 1698 2383 3376 3567 33
Size: 178 991 496 685 993 191 1121
Fragments arranged by size:
1121 993 991 685 496 191 178
SfoI GGC'GCC
Cuts at: 2087 2335 2087
Size: 248 4407
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4655
SmaI CCC'GGG
Cuts at: 1677 1677
Size: 4655
SmlI C'TyrA_G
Cuts at: 1810 2725 3217 3479 1810
Size: 915 492 262 2986
Fragments arranged by size:
2986 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4655
SphI G_CATG'C
Cuts at: 1708 2208 1708
Size: 500 4155
StyI C'CwwG_G
Cuts at: 107 1509 1789 2143 107
Size: 1402 280 354 2619
Fragments arranged by size:
2619 1402 354 280
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2238 2371 2830 3039 2238
Size: 133 459 209 3854
Fragments arranged by size:
3854 459 209 133
TatI w'GTAC_w
Cuts at: 147 516 936 1553 4170 147
Size: 369 420 617 2617 632
Fragments arranged by size:
2617 632 617 420 369
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1371 1403 4172 4204 1371
Size: 32 2769 32 1822
Fragments arranged by size:
2769 1822 32 32
Tth111I GACn'n_nGTC
Cuts at: 2452 2452
Size: 4655
XbaI T'CTAG_A
Cuts at: 1686 1686
Size: 4655
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 374 2150 374
Size: 1776 2879
XmaI C'CCGG_G
Cuts at: 1675 1675
Size: 4655
XmnI GAAnn'nnTTC
Cuts at: 851 1426 1629 851
Size: 575 203 3877
Fragments arranged by size:
3877 575 203
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglII BlpI Bme1580I
BmrI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PciI PflMI PfoI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BglI BmgBI BmtI
BpmI BseRI BsgI BsiWI BsmBI BssHII BstEII BstXI
BstZ17I ClaI CspCI DraIII EcoNI EcoRV FseI FspAI
HpaI MfeI NdeI NheI NotI NsiI PacI PasI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
ZraI