Restriction map for
pF1KB7785
(Circular) (Six-base) MAPSORT of: pF1KB7785.seq Check: 6636 from: 1 to: 4484
pF1KB7785 4484 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 621 1522 621
Size: 901 3583
Acc65I G'GTAC_C
Cuts at: 1500 1500
Size: 4484
AclI AA'CG_TT
Cuts at: 4324 4324
Size: 4484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 1272 1356 2309 2741 3488 3756 160
Size: 1112 84 953 432 747 268 888
Fragments arranged by size:
1112 953 888 747 432 268 84
AflIII A'CryG_T
Cuts at: 1836 2940 3917 1836
Size: 1104 977 2403
Fragments arranged by size:
2403 1104 977
AgeI A'CCGG_T
Cuts at: 2872 2872
Size: 4484
AlwNI CAG_nnn'CTG
Cuts at: 1789 3356 1789
Size: 1567 2917
ApaLI G'TGCA_C
Cuts at: 3254 3254
Size: 4484
ApoI r'AATT_y
Cuts at: 425 902 1154 1440 1488 3837 4093 425
Size: 477 252 286 48 2349 256 816
Fragments arranged by size:
2349 816 477 286 256 252 48
AseI AT'TA_AT
Cuts at: 20 20
Size: 4484
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
AvaI C'yCGr_G
Cuts at: 1504 1504
Size: 4484
BamHI G'GATC_C
Cuts at: 1509 1509
Size: 4484
BanI G'GyrC_C
Cuts at: 1500 1914 2162 2197 1500
Size: 414 248 35 3787
Fragments arranged by size:
3787 414 248 35
BanII G_rGCy'C
Cuts at: 1384 1498 2528 1384
Size: 114 1030 3340
Fragments arranged by size:
3340 1030 114
BbeI G_GCGC'C
Cuts at: 1918 2166 1918
Size: 248 4236
BciVI GTATCCnnnnn_n'
Cuts at: 2382 3143 4484 2382
Size: 761 1341 2382
Fragments arranged by size:
2382 1341 761
BclI T'GATC_A
Cuts at: 413 2004 413
Size: 1591 2893
BfrBI ATG'CAT
Cuts at: 676 1016 1216 676
Size: 340 200 3944
Fragments arranged by size:
3944 340 200
BglII A'GATC_T
Cuts at: 327 1999 4036 327
Size: 1672 2037 775
Fragments arranged by size:
2037 1672 775
BlpI GC'TnA_GC
Cuts at: 1596 1596
Size: 4484
Bme1580I G_kGCm'C
Cuts at: 2109 2202 3258 2109
Size: 93 1056 3335
Fragments arranged by size:
3335 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 250 399 836 1696 1777 2112 4256 250
Size: 149 437 860 81 335 2144 478
Fragments arranged by size:
2144 860 478 437 335 149 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1189 2851 1189
Size: 1662 2822
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 680 1016 1184 1268 1352 1398 680
Size: 336 168 84 84 46 3766
Fragments arranged by size:
3766 336 168 84 84 46
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1660 2539 3031 3329 1660
Size: 879 492 298 2815
Fragments arranged by size:
2815 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 240 4188 240
Size: 3948 536
BsaAI yAC'GTr
Cuts at: 1837 1837
Size: 4484
BsaBI GATnn'nnATC
Cuts at: 6 1998 4035 6
Size: 1992 2037 455
Fragments arranged by size:
2037 1992 455
BsaHI Gr'CG_yC
Cuts at: 1915 2163 2865 4361 1915
Size: 248 702 1496 2038
Fragments arranged by size:
2038 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1673 2194 2872 3146 3293 3704 1673
Size: 521 678 274 147 411 2453
Fragments arranged by size:
2453 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 175 175
Size: 4484
BseYI C'CCAG_C
Cuts at: 303 1909 2667 3244 3740 303
Size: 1606 758 577 496 1047
Fragments arranged by size:
1606 1047 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 2026 2072 3280 104
Size: 1922 46 1208 1308
Fragments arranged by size:
1922 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1384 1498 2276 2466 3258 1384
Size: 114 778 190 792 2610
Fragments arranged by size:
2610 792 778 190 114
BsmI GAATG_Cn'
Cuts at: 1018 1018
Size: 4484
Bsp1286I G_dGCh'C
Cuts at: 1384 1498 2109 2202 2276 2466 2528 3258 1384
Size: 114 611 93 74 190 62 730 2610
Fragments arranged by size:
2610 730 611 190 114 93 74 62
BspEI T'CCGG_A
Cuts at: 3704 3704
Size: 4484
BspHI T'CATG_A
Cuts at: 3660 3660
Size: 4484
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1534 1748 2050 2431 1534
Size: 214 302 381 3587
Fragments arranged by size:
3587 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2776 2830 4314 4479 50
Size: 2726 54 1484 165 55
Fragments arranged by size:
2726 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2396 2396
Size: 4484
BsrFI r'CCGG_y
Cuts at: 2482 2663 2872 2482
Size: 181 209 4094
Fragments arranged by size:
4094 209 181
BsrGI T'GTAC_A
Cuts at: 197 197
Size: 4484
BssSI C'ACGA_G
Cuts at: 2755 3113 2755
Size: 358 4126
BstAPI GCAn_nnn'nTGC
Cuts at: 1861 1861
Size: 4484
BstBI TT'CG_AA
Cuts at: 2845 2845
Size: 4484
BstXI CCAn_nnnn'nTGG
Cuts at: 604 604
Size: 4484
BstYI r'GATC_y
Cuts at: 1 327 1509 1976 1999 2334 2580 3592 3678
Size: 326 1182 467 23 335 246 1012 86
Cuts at: 3678 3690 4036 1
Size: 12 346 449
Fragments arranged by size:
1182 1012 467 449 346 335 326 246
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2434 2615 2778 2434
Size: 181 163 4140
Fragments arranged by size:
4140 181 163
BtsI GCAGTG_nn'
Cuts at: 963 1727 963
Size: 764 3720
DraI TTT'AAA
Cuts at: 459 1483 459
Size: 1024 3460
DrdI GACnn_nn'nnGTC
Cuts at: 2190 3048 2190
Size: 858 3626
EaeI y'GGCC_r
Cuts at: 140 225 2069 2243 2634 2661 4006 140
Size: 85 1844 174 391 27 1345 618
Fragments arranged by size:
1844 1345 618 391 174 85 27
EagI C'GGCC_G
Cuts at: 2069 2069
Size: 4484
EarI CTCTTCn'nnn_
Cuts at: 1231 2507 2717 1231
Size: 1276 210 2998
Fragments arranged by size:
2998 1276 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2692 3002 3148 4294 2692
Size: 310 146 1146 2882
Fragments arranged by size:
2882 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1496 1382
Size: 114 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 680 1016 1184 1268 1272 1352 1356 1398
Size: 520 336 168 84 4 80 4 42
Cuts at: 1398 2309 2741 3488 3756 160
Size: 911 432 747 268 888
Fragments arranged by size:
911 888 747 520 432 336 268 168
84 80 42 4 4
EcoO109I rG'GnC_Cy
Cuts at: 439 584 1623 439
Size: 145 1039 3300
Fragments arranged by size:
3300 1039 145
EcoRI G'AATT_C
Cuts at: 425 902 1154 1488 425
Size: 477 252 334 3421
Fragments arranged by size:
3421 477 334 252
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2503 2503
Size: 4484
FspI TGC'GCA
Cuts at: 2265 2265
Size: 4484
HaeII r_GCGC'y
Cuts at: 1918 2166 3188 4167 1918
Size: 248 1022 979 2235
Fragments arranged by size:
2235 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 385 417 1398 1430 2326 2356 2358 2388 385
Size: 32 981 32 896 30 2 30 2481
Fragments arranged by size:
2481 981 896 32 32 30 30 2
HincII GTy'rAC
Cuts at: 622 1523 622
Size: 901 3583
HindIII A'AGCT_T
Cuts at: 1115 1115
Size: 4484
KasI G'GCGC_C
Cuts at: 1914 2162 1914
Size: 248 4236
KpnI G_GTAC'C
Cuts at: 1504 1504
Size: 4484
MluI A'CGCG_T
Cuts at: 3917 3917
Size: 4484
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 194 905 3155 3339 194
Size: 711 2250 184 1339
Fragments arranged by size:
2250 1339 711 184
MscI TGG'CCA
Cuts at: 142 227 2245 142
Size: 85 2018 2381
Fragments arranged by size:
2381 2018 85
MslI CAynn'nnrTG
Cuts at: 602 675 2473 2600 602
Size: 73 1798 127 2486
Fragments arranged by size:
2486 1798 127 73
MspA1I CmG'CkG
Cuts at: 1376 1595 1909 2269 3282 3527 1376
Size: 219 314 360 1013 245 2333
Fragments arranged by size:
2333 1013 360 314 245 219
NaeI GCC'GGC
Cuts at: 2665 2665
Size: 4484
NarI GG'CG_CC
Cuts at: 1915 2163 1915
Size: 248 4236
NdeI CA'TA_TG
Cuts at: 560 672 560
Size: 112 4372
NgoMIV G'CCGG_C
Cuts at: 2663 2663
Size: 4484
NsiI A_TGCA'T
Cuts at: 678 1018 1218 678
Size: 340 200 3944
Fragments arranged by size:
3944 340 200
NspI r_CATG'y
Cuts at: 680 1216 1537 2037 2944 680
Size: 536 321 500 907 2220
Fragments arranged by size:
2220 907 536 500 321
PciI A'CATG_T
Cuts at: 2940 2940
Size: 4484
PmeI GTTT'AAAC
Cuts at: 1483 1483
Size: 4484
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3623 3655 3623
Size: 32 4452
PstI C_TGCA'G
Cuts at: 1531 2216 1531
Size: 685 3799
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4484
PvuII CAG'CTG
Cuts at: 1376 1909 2269 1376
Size: 533 360 3591
Fragments arranged by size:
3591 533 360
RsrII CG'GwC_CG
Cuts at: 2679 2679
Size: 4484
SacI G_AGCT'C
Cuts at: 1384 1498 1384
Size: 114 4370
SalI G'TCGA_C
Cuts at: 620 1521 620
Size: 901 3583
SapI GCTCTTCn'nnn_
Cuts at: 2507 2717 2507
Size: 210 4274
SbfI CC_TGCA'GG
Cuts at: 1531 1531
Size: 4484
ScaI AGT'ACT
Cuts at: 573 4001 573
Size: 3428 1056
SexAI A'CCwGG_T
Cuts at: 1475 1475
Size: 4484
SfcI C'TryA_G
Cuts at: 33 1527 2212 3205 3396 33
Size: 1494 685 993 191 1121
Fragments arranged by size:
1494 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1916 2164 1916
Size: 248 4236
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4484
SmaI CCC'GGG
Cuts at: 1506 1506
Size: 4484
SmlI C'TyrA_G
Cuts at: 1639 2554 3046 3308 1639
Size: 915 492 262 2815
Fragments arranged by size:
2815 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4484
SphI G_CATG'C
Cuts at: 1216 1537 2037 1216
Size: 321 500 3663
Fragments arranged by size:
3663 500 321
StuI AGG'CCT
Cuts at: 865 865
Size: 4484
StyI C'CwwG_G
Cuts at: 1618 1972 1618
Size: 354 4130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2067 2200 2659 2868 2067
Size: 133 459 209 3683
Fragments arranged by size:
3683 459 209 133
TatI w'GTAC_w
Cuts at: 197 571 847 3999 197
Size: 374 276 3152 682
Fragments arranged by size:
3152 682 374 276
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4001 4033 4001
Size: 32 4452
Tth111I GACn'n_nGTC
Cuts at: 2281 2281
Size: 4484
XbaI T'CTAG_A
Cuts at: 1515 1515
Size: 4484
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1979 1979
Size: 4484
XmaI C'CCGG_G
Cuts at: 1504 1504
Size: 4484
XmnI GAAnn'nnTTC
Cuts at: 425 971 1117 425
Size: 546 146 3792
Fragments arranged by size:
3792 546 146
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BciVI BclI BfrBI BglII BlpI Bme1580I BmrI BplI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI
BstYI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NsiI NspI
PciI PmeI PpiI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BcgI BglI
BmgBI BmtI Bpu10I BsaXI BsgI BsiWI BsmBI BssHII
BstEII BstZ17I Bsu36I BtgI ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI PacI PasI PflMI PfoI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI ZraI