Restriction map for
pF1KB7770
(Circular) (Six-base) MAPSORT of: pF1KB7770.seq Check: 2162 from: 1 to: 4544
pF1KB7770 4544 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1458 1458
Size: 4544
AccI GT'mk_AC
Cuts at: 1582 1582
Size: 4544
Acc65I G'GTAC_C
Cuts at: 1560 1560
Size: 4544
AclI AA'CG_TT
Cuts at: 4384 4384
Size: 4544
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1390 2369 2801 3548 3816 1390
Size: 979 432 747 268 2118
Fragments arranged by size:
2118 979 747 432 268
AflIII A'CryG_T
Cuts at: 1452 1896 3000 3977 1452
Size: 444 1104 977 2019
Fragments arranged by size:
2019 1104 977 444
AgeI A'CCGG_T
Cuts at: 2932 2932
Size: 4544
AhdI GACnn_n'nnGTC
Cuts at: 1441 1441
Size: 4544
AlwNI CAG_nnn'CTG
Cuts at: 1464 1849 3416 1464
Size: 385 1567 2592
Fragments arranged by size:
2592 1567 385
ApaI G_GGCC'C
Cuts at: 747 747
Size: 4544
ApaLI G'TGCA_C
Cuts at: 3314 3314
Size: 4544
ApoI r'AATT_y
Cuts at: 864 1548 3897 4153 864
Size: 684 2349 256 1255
Fragments arranged by size:
2349 1255 684 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4544
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
AvaI C'yCGr_G
Cuts at: 1564 1564
Size: 4544
BamHI G'GATC_C
Cuts at: 566 1569 566
Size: 1003 3541
BanI G'GyrC_C
Cuts at: 1560 1974 2222 2257 1560
Size: 414 248 35 3847
Fragments arranged by size:
3847 414 248 35
BanII G_rGCy'C
Cuts at: 747 1205 1364 1558 2588 747
Size: 458 159 194 1030 2703
Fragments arranged by size:
2703 1030 458 194 159
BbeI G_GCGC'C
Cuts at: 1978 2226 1978
Size: 248 4296
BbsI GAAGACnn'nnnn_
Cuts at: 191 266 385 1334 1486 1492 191
Size: 75 119 949 152 6 3243
Fragments arranged by size:
3243 949 152 119 75 6
BciVI GTATCCnnnnn_n'
Cuts at: 2442 3203 4544 2442
Size: 761 1341 2442
Fragments arranged by size:
2442 1341 761
BclI T'GATC_A
Cuts at: 1472 2064 1472
Size: 592 3952
BglI GCCn_nnn'nGGC
Cuts at: 138 138
Size: 4544
BglII A'GATC_T
Cuts at: 695 1520 2059 4096 695
Size: 825 539 2037 1143
Fragments arranged by size:
2037 1143 825 539
BlpI GC'TnA_GC
Cuts at: 1656 1656
Size: 4544
Bme1580I G_kGCm'C
Cuts at: 747 2169 2262 3318 747
Size: 1422 93 1056 1973
Fragments arranged by size:
1973 1422 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1756 1837 2172 4316 1756
Size: 81 335 2144 1984
Fragments arranged by size:
2144 1984 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 2911
Size: 4544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 684 1348 1378 1399 219
Size: 465 664 30 21 3364
Fragments arranged by size:
3364 664 465 30 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1720 2599 3091 3389 1720
Size: 879 492 298 2875
Fragments arranged by size:
2875 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 1286 4248 1286
Size: 2962 1582
BsaAI yAC'GTr
Cuts at: 1455 1897 1455
Size: 442 4102
BsaBI GATnn'nnATC
Cuts at: 6 2058 4095 6
Size: 2052 2037 455
Fragments arranged by size:
2052 2037 455
BsaHI Gr'CG_yC
Cuts at: 1975 2223 2925 4421 1975
Size: 248 702 1496 2098
Fragments arranged by size:
2098 1496 702 248
BsaWI w'CCGG_w
Cuts at: 512 1733 2254 2932 3206 3353 3764 512
Size: 1221 521 678 274 147 411 1292
Fragments arranged by size:
1292 1221 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 181 211 670 700 1192 1222 181
Size: 30 459 30 492 30 3503
Fragments arranged by size:
3503 492 459 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1048 1048
Size: 4544
BseYI C'CCAG_C
Cuts at: 1969 2727 3304 3800 1969
Size: 758 577 496 2713
Fragments arranged by size:
2713 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1006 1477 1006
Size: 471 4073
BsiEI CG_ry'CG
Cuts at: 104 2086 2132 3340 104
Size: 1982 46 1208 1308
Fragments arranged by size:
1982 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1364 1558 2336 2526 3318 1364
Size: 194 778 190 792 2590
Fragments arranged by size:
2590 792 778 194 190
BsmI GAATG_Cn'
Cuts at: 1013 1013
Size: 4544
Bsp1286I G_dGCh'C
Cuts at: 747 1205 1364 1558 2169 2262 2336 2526 2588
Size: 458 159 194 611 93 74 190 62
Cuts at: 2588 3318 747
Size: 730 1973
Fragments arranged by size:
1973 730 611 458 194 190 159 93
74 62
BspEI T'CCGG_A
Cuts at: 512 3764 512
Size: 3252 1292
BspHI T'CATG_A
Cuts at: 402 1148 3720 402
Size: 746 2572 1226
Fragments arranged by size:
2572 1226 746
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1352 1458 1594 1808 2110 2491 1352
Size: 106 136 214 302 381 3405
Fragments arranged by size:
3405 381 302 214 136 106
BsrBI CCG'CTC
Cuts at: 50 1509 2836 2890 4374 4539 50
Size: 1459 1327 54 1484 165 55
Fragments arranged by size:
1484 1459 1327 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2456 2456
Size: 4544
BsrFI r'CCGG_y
Cuts at: 2542 2723 2932 2542
Size: 181 209 4154
Fragments arranged by size:
4154 209 181
BssSI C'ACGA_G
Cuts at: 1075 2815 3173 1075
Size: 1740 358 2446
Fragments arranged by size:
2446 1740 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1464 1921 1464
Size: 457 4087
BstBI TT'CG_AA
Cuts at: 2905 2905
Size: 4544
BstXI CCAn_nnnn'nTGG
Cuts at: 114 114
Size: 4544
BstYI r'GATC_y
Cuts at: 1 168 282 566 695 1520 1569 2036 2059
Size: 167 114 284 129 825 49 467 23
Cuts at: 2059 2394 2640 3652 3738 3750 4096 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1012 825 467 449 346 335 284 246
167 129 114 86 49 23 12
BtgI C'CryG_G
Cuts at: 113 164 974 113
Size: 51 810 3683
Fragments arranged by size:
3683 810 51
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2494 2675 2838 2494
Size: 181 163 4200
Fragments arranged by size:
4200 181 163
BtsI GCAGTG_nn'
Cuts at: 629 1787 629
Size: 1158 3386
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 132 167 326 361 132
Size: 35 159 35 4315
Fragments arranged by size:
4315 159 35 35
DraI TTT'AAA
Cuts at: 1543 1543
Size: 4544
DrdI GACnn_nn'nnGTC
Cuts at: 2250 3108 2250
Size: 858 3686
EaeI y'GGCC_r
Cuts at: 938 2129 2303 2694 2721 4066 938
Size: 1191 174 391 27 1345 1416
Fragments arranged by size:
1416 1345 1191 391 174 27
EagI C'GGCC_G
Cuts at: 2129 2129
Size: 4544
EarI CTCTTCn'nnn_
Cuts at: 401 2567 2777 401
Size: 2166 210 2168
Fragments arranged by size:
2168 2166 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 672 706 2752 3062 3208 4354 672
Size: 34 2046 310 146 1146 862
Fragments arranged by size:
2046 1146 862 310 146 34
EcoICRI GAG'CTC
Cuts at: 1362 1556 1362
Size: 194 4350
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 219 684 1348 1378 1390 1399 2369 2801 3548
Size: 465 664 30 12 9 970 432 747
Cuts at: 3548 3816 219
Size: 268 947
Fragments arranged by size:
970 947 747 664 465 432 268 30
12 9
EcoO109I rG'GnC_Cy
Cuts at: 744 1683 744
Size: 939 3605
EcoRI G'AATT_C
Cuts at: 864 1548 864
Size: 684 3860
EcoRV GAT'ATC
Cuts at: 207 708 207
Size: 501 4043
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 525 2563 525
Size: 2038 2506
FspI TGC'GCA
Cuts at: 2325 2325
Size: 4544
HaeII r_GCGC'y
Cuts at: 1978 2226 3248 4227 1978
Size: 248 1022 979 2295
Fragments arranged by size:
2295 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 340 372 1427 1459 2386 2416 2418 2448 340
Size: 32 1055 32 927 30 2 30 2436
Fragments arranged by size:
2436 1055 927 32 32 30 30 2
HincII GTy'rAC
Cuts at: 120 228 1583 120
Size: 108 1355 3081
Fragments arranged by size:
3081 1355 108
HindIII A'AGCT_T
Cuts at: 458 532 458
Size: 74 4470
KasI G'GCGC_C
Cuts at: 1974 2222 1974
Size: 248 4296
KpnI G_GTAC'C
Cuts at: 1564 1564
Size: 4544
MfeI C'AATT_G
Cuts at: 431 431
Size: 4544
MluI A'CGCG_T
Cuts at: 3977 3977
Size: 4544
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 440 544 3215 3399 440
Size: 104 2671 184 1585
Fragments arranged by size:
2671 1585 184 104
MscI TGG'CCA
Cuts at: 940 2305 940
Size: 1365 3179
MslI CAynn'nnrTG
Cuts at: 112 127 488 761 2533 2660 112
Size: 15 361 273 1772 127 1996
Fragments arranged by size:
1996 1772 361 273 127 15
MspA1I CmG'CkG
Cuts at: 717 733 789 1368 1398 1655 1969 2329 3342
Size: 16 56 579 30 257 314 360 1013
Cuts at: 3342 3587 717
Size: 245 1674
Fragments arranged by size:
1674 1013 579 360 314 257 245 56
30 16
NaeI GCC'GGC
Cuts at: 2725 2725
Size: 4544
NarI GG'CG_CC
Cuts at: 1975 2223 1975
Size: 248 4296
NcoI C'CATG_G
Cuts at: 113 113
Size: 4544
NgoMIV G'CCGG_C
Cuts at: 2723 2723
Size: 4544
NspI r_CATG'y
Cuts at: 1597 2097 3004 1597
Size: 500 907 3137
Fragments arranged by size:
3137 907 500
PciI A'CATG_T
Cuts at: 3000 3000
Size: 4544
PmeI GTTT'AAAC
Cuts at: 1543 1543
Size: 4544
PmlI CAC'GTG
Cuts at: 1455 1455
Size: 4544
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3683 3715 3683
Size: 32 4512
PshAI GACnn'nnGTC
Cuts at: 1496 1496
Size: 4544
PspOMI G'GGCC_C
Cuts at: 743 743
Size: 4544
PstI C_TGCA'G
Cuts at: 920 1469 1517 1591 2276 920
Size: 549 48 74 685 3188
Fragments arranged by size:
3188 685 549 74 48
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4544
PvuII CAG'CTG
Cuts at: 717 1368 1398 1969 2329 717
Size: 651 30 571 360 2932
Fragments arranged by size:
2932 651 571 360 30
RsrII CG'GwC_CG
Cuts at: 2739 2739
Size: 4544
SacI G_AGCT'C
Cuts at: 1364 1558 1364
Size: 194 4350
SalI G'TCGA_C
Cuts at: 1581 1581
Size: 4544
SapI GCTCTTCn'nnn_
Cuts at: 401 2567 2777 401
Size: 2166 210 2168
Fragments arranged by size:
2168 2166 210
SbfI CC_TGCA'GG
Cuts at: 920 1517 1591 920
Size: 597 74 3873
Fragments arranged by size:
3873 597 74
ScaI AGT'ACT
Cuts at: 598 4061 598
Size: 3463 1081
SfcI C'TryA_G
Cuts at: 33 916 1465 1513 1587 2272 3265 3456 33
Size: 883 549 48 74 685 993 191 1121
Fragments arranged by size:
1121 993 883 685 549 191 74 48
SfoI GGC'GCC
Cuts at: 1976 2224 1976
Size: 248 4296
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4544
SmaI CCC'GGG
Cuts at: 1566 1566
Size: 4544
SmlI C'TyrA_G
Cuts at: 1699 2614 3106 3368 1699
Size: 915 492 262 2875
Fragments arranged by size:
2875 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4544
SphI G_CATG'C
Cuts at: 1597 2097 1597
Size: 500 4044
SspI AAT'ATT
Cuts at: 1265 1265
Size: 4544
StyI C'CwwG_G
Cuts at: 113 1140 1678 2032 113
Size: 1027 538 354 2625
Fragments arranged by size:
2625 1027 538 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2127 2260 2719 2928 2127
Size: 133 459 209 3743
Fragments arranged by size:
3743 459 209 133
TatI w'GTAC_w
Cuts at: 596 4059 596
Size: 3463 1081
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 181 213 1372 1404 4061 4093 181
Size: 32 1159 32 2657 32 632
Fragments arranged by size:
2657 1159 632 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2341 2341
Size: 4544
XbaI T'CTAG_A
Cuts at: 1575 1575
Size: 4544
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 423 2039 423
Size: 1616 2928
XmaI C'CCGG_G
Cuts at: 1564 1564
Size: 4544
XmnI GAAnn'nnTTC
Cuts at: 263 1331 263
Size: 1068 3476
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI CspCI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI
PciI PmeI PmlI PpiI PshAI PspOMI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AlfI AloI AscI AvrII
BaeI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII
EcoNI FseI FspAI HpaI NdeI NheI NotI NruI
NsiI PacI PasI PflMI PfoI PpuMI PsiI PspXI
PsrI SacII SanDI SexAI SfiI SgrAI SnaBI SrfI
StuI SwaI XhoI ZraI