Restriction map for
pF1KB7748
(Circular) (Six-base) MAPSORT of: pF1KB7748.seq Check: 8116 from: 1 to: 4577
pF1KB7748 4577 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1615 1615
Size: 4577
Acc65I G'GTAC_C
Cuts at: 1593 1593
Size: 4577
AclI AA'CG_TT
Cuts at: 4417 4417
Size: 4577
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 1238 1398 2402 2834 3581 3849 132
Size: 1106 160 1004 432 747 268 860
Fragments arranged by size:
1106 1004 860 747 432 268 160
AfeI AGC'GCT
Cuts at: 1527 1527
Size: 4577
AflIII A'CryG_T
Cuts at: 869 1929 3033 4010 869
Size: 1060 1104 977 1436
Fragments arranged by size:
1436 1104 1060 977
AgeI A'CCGG_T
Cuts at: 718 2965 718
Size: 2247 2330
AhdI GACnn_n'nnGTC
Cuts at: 1251 1251
Size: 4577
AleI CACnn'nnGTG
Cuts at: 397 593 397
Size: 196 4381
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1287 1319 1287
Size: 32 4545
AlwNI CAG_nnn'CTG
Cuts at: 155 441 1882 3449 155
Size: 286 1441 1567 1283
Fragments arranged by size:
1567 1441 1283 286
ApaLI G'TGCA_C
Cuts at: 739 3347 739
Size: 2608 1969
ApoI r'AATT_y
Cuts at: 631 661 843 1581 3930 4186 631
Size: 30 182 738 2349 256 1022
Fragments arranged by size:
2349 1022 738 256 182 30
AseI AT'TA_AT
Cuts at: 20 20
Size: 4577
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
AvaI C'yCGr_G
Cuts at: 533 561 1540 1597 533
Size: 28 979 57 3513
Fragments arranged by size:
3513 979 57 28
BamHI G'GATC_C
Cuts at: 1602 1602
Size: 4577
BanI G'GyrC_C
Cuts at: 405 541 1345 1483 1593 2007 2255 2290 405
Size: 136 804 138 110 414 248 35 2692
Fragments arranged by size:
2692 804 414 248 138 136 110 35
BanII G_rGCy'C
Cuts at: 340 1173 1207 1591 2621 340
Size: 833 34 384 1030 2296
Fragments arranged by size:
2296 1030 833 384 34
BbeI G_GCGC'C
Cuts at: 409 545 1349 1487 2011 2259 409
Size: 136 804 138 524 248 2727
Fragments arranged by size:
2727 804 524 248 138 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 290 324 790 824 290
Size: 34 466 34 4043
Fragments arranged by size:
4043 466 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2475 3236 4577 2475
Size: 761 1341 2475
Fragments arranged by size:
2475 1341 761
BclI T'GATC_A
Cuts at: 2097 2097
Size: 4577
BglI GCCn_nnn'nGGC
Cuts at: 124 191 1475 124
Size: 67 1284 3226
Fragments arranged by size:
3226 1284 67
BglII A'GATC_T
Cuts at: 699 2092 4129 699
Size: 1393 2037 1147
Fragments arranged by size:
2037 1393 1147
BlpI GC'TnA_GC
Cuts at: 1689 1689
Size: 4577
Bme1580I G_kGCm'C
Cuts at: 743 2202 2295 3351 743
Size: 1459 93 1056 1969
Fragments arranged by size:
1969 1459 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 391 1789 1870 2205 4349 391
Size: 1398 81 335 2144 619
Fragments arranged by size:
2144 1398 619 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2944 2944
Size: 4577
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 600 1000 600
Size: 400 4177
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1753 2632 3124 3422 1753
Size: 879 492 298 2908
Fragments arranged by size:
2908 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4281 4281
Size: 4577
BsaAI yAC'GTr
Cuts at: 1008 1930 1008
Size: 922 3655
BsaBI GATnn'nnATC
Cuts at: 6 2091 4128 6
Size: 2085 2037 455
Fragments arranged by size:
2085 2037 455
BsaHI Gr'CG_yC
Cuts at: 406 542 1346 1484 2008 2256 2958 4454 406
Size: 136 804 138 524 248 702 1496 529
Fragments arranged by size:
1496 804 702 529 524 248 138 136
BsaWI w'CCGG_w
Cuts at: 718 1766 2287 2965 3239 3386 3797 718
Size: 1048 521 678 274 147 411 1498
Fragments arranged by size:
1498 1048 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 973 1003 1287 1317 973
Size: 30 284 30 4233
Fragments arranged by size:
4233 284 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 165 1215 165
Size: 1050 3527
BseYI C'CCAG_C
Cuts at: 1044 1207 1270 1369 2002 2760 3337 3833 1044
Size: 163 63 99 633 758 577 496 1788
Fragments arranged by size:
1788 758 633 577 496 163 99 63
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 241 1294 241
Size: 1053 3524
BsiEI CG_ry'CG
Cuts at: 104 164 182 314 767 2119 2165 3373 104
Size: 60 18 132 453 1352 46 1208 1308
Fragments arranged by size:
1352 1308 1208 453 132 60 46 18
BsiHKAI G_wGCw'C
Cuts at: 743 896 1591 2369 2559 3351 743
Size: 153 695 778 190 792 1969
Fragments arranged by size:
1969 792 778 695 190 153
Bsp1286I G_dGCh'C
Cuts at: 340 743 896 1173 1207 1591 2202 2295 2369
Size: 403 153 277 34 384 611 93 74
Cuts at: 2369 2559 2621 3351 340
Size: 190 62 730 1566
Fragments arranged by size:
1566 730 611 403 384 277 190 153
93 74 62 34
BspEI T'CCGG_A
Cuts at: 3797 3797
Size: 4577
BspHI T'CATG_A
Cuts at: 3753 3753
Size: 4577
BspMI ACCTGCnnnn'nnnn_
Cuts at: 971 1627 1841 2143 2524 971
Size: 656 214 302 381 3024
Fragments arranged by size:
3024 656 381 302 214
BsrBI CCG'CTC
Cuts at: 50 450 1284 2869 2923 4407 4572 50
Size: 400 834 1585 54 1484 165 55
Fragments arranged by size:
1585 1484 834 400 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2489 2489
Size: 4577
BsrFI r'CCGG_y
Cuts at: 181 262 718 766 1085 2575 2756 2965 181
Size: 81 456 48 319 1490 181 209 1793
Fragments arranged by size:
1793 1490 456 319 209 181 81 48
BssHII G'CGCG_C
Cuts at: 945 1556 945
Size: 611 3966
BssSI C'ACGA_G
Cuts at: 2848 3206 2848
Size: 358 4219
BstAPI GCAn_nnn'nTGC
Cuts at: 1954 1954
Size: 4577
BstBI TT'CG_AA
Cuts at: 2938 2938
Size: 4577
BstXI CCAn_nnnn'nTGG
Cuts at: 399 595 399
Size: 196 4381
BstYI r'GATC_y
Cuts at: 1 273 376 699 789 1602 2069 2092 2427
Size: 272 103 323 90 813 467 23 335
Cuts at: 2427 2673 3685 3771 3783 4129 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 813 467 449 346 335 323 272
246 103 90 86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 228 1054 2527 2708 2871 228
Size: 826 1473 181 163 1934
Fragments arranged by size:
1934 1473 826 181 163
BtsI GCAGTG_nn'
Cuts at: 1371 1820 1371
Size: 449 4128
DraI TTT'AAA
Cuts at: 1576 1576
Size: 4577
DrdI GACnn_nn'nnGTC
Cuts at: 673 2283 3141 673
Size: 1610 858 2109
Fragments arranged by size:
2109 1610 858
EaeI y'GGCC_r
Cuts at: 161 179 311 371 764 768 2162 2336 2727
Size: 18 132 60 393 4 1394 174 391
Cuts at: 2727 2754 4099 161
Size: 27 1345 639
Fragments arranged by size:
1394 1345 639 393 391 174 132 60
27 18 4
EagI C'GGCC_G
Cuts at: 161 179 311 764 2162 161
Size: 18 132 453 1398 2576
Fragments arranged by size:
2576 1398 453 132 18
EarI CTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 512 518 1104 2785 3095 3241 4387 512
Size: 6 586 1681 310 146 1146 702
Fragments arranged by size:
1681 1146 702 586 310 146 6
EcoICRI GAG'CTC
Cuts at: 1589 1589
Size: 4577
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 132 600 1000 1238 1398 2402 2834 3581 3849
Size: 468 400 238 160 1004 432 747 268
Cuts at: 3849 132
Size: 860
Fragments arranged by size:
1004 860 747 468 432 400 268 238
160
EcoO109I rG'GnC_Cy
Cuts at: 294 305 880 1332 1716 294
Size: 11 575 452 384 3155
Fragments arranged by size:
3155 575 452 384 11
EcoRI G'AATT_C
Cuts at: 1581 1581
Size: 4577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 252 1576 2596 252
Size: 1324 1020 2233
Fragments arranged by size:
2233 1324 1020
FseI GG_CCGG'CC
Cuts at: 185 770 185
Size: 585 3992
FspI TGC'GCA
Cuts at: 2358 2358
Size: 4577
HaeII r_GCGC'y
Cuts at: 409 545 1349 1424 1487 1529 2011 2259 3281
Size: 136 804 75 63 42 482 248 1022
Cuts at: 3281 4260 409
Size: 979 726
Fragments arranged by size:
1022 979 804 726 482 248 136 75
63 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1144 1176 1237 1269 2419 2449 2451 2481 1144
Size: 32 61 32 1150 30 2 30 3240
Fragments arranged by size:
3240 1150 61 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1616 1616
Size: 4577
KasI G'GCGC_C
Cuts at: 405 541 1345 1483 2007 2255 405
Size: 136 804 138 524 248 2727
Fragments arranged by size:
2727 804 524 248 138 136
KpnI G_GTAC'C
Cuts at: 1597 1597
Size: 4577
MluI A'CGCG_T
Cuts at: 4010 4010
Size: 4577
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 167 983 3248 3432 167
Size: 816 2265 184 1312
Fragments arranged by size:
2265 1312 816 184
MscI TGG'CCA
Cuts at: 2338 2338
Size: 4577
MslI CAynn'nnrTG
Cuts at: 397 593 2566 2693 397
Size: 196 1973 127 2281
Fragments arranged by size:
2281 1973 196 127
MspA1I CmG'CkG
Cuts at: 438 1032 1388 1688 2002 2362 3375 3620 438
Size: 594 356 300 314 360 1013 245 1395
Fragments arranged by size:
1395 1013 594 360 356 314 300 245
NaeI GCC'GGC
Cuts at: 183 264 768 2758 183
Size: 81 504 1990 2002
Fragments arranged by size:
2002 1990 504 81
NarI GG'CG_CC
Cuts at: 406 542 1346 1484 2008 2256 406
Size: 136 804 138 524 248 2727
Fragments arranged by size:
2727 804 524 248 138 136
NgoMIV G'CCGG_C
Cuts at: 181 262 766 2756 181
Size: 81 504 1990 2002
Fragments arranged by size:
2002 1990 504 81
NspI r_CATG'y
Cuts at: 1630 2130 3037 1630
Size: 500 907 3170
Fragments arranged by size:
3170 907 500
PciI A'CATG_T
Cuts at: 3033 3033
Size: 4577
PflMI CCAn_nnn'nTGG
Cuts at: 1165 1165
Size: 4577
PmeI GTTT'AAAC
Cuts at: 1576 1576
Size: 4577
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1287 1319 3716 3748 1287
Size: 32 2397 32 2116
Fragments arranged by size:
2397 2116 32 32
PpuMI rG'GwC_Cy
Cuts at: 880 880
Size: 4577
PstI C_TGCA'G
Cuts at: 551 660 758 968 1624 2309 551
Size: 109 98 210 656 685 2819
Fragments arranged by size:
2819 685 656 210 109 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4577
PvuII CAG'CTG
Cuts at: 1032 2002 2362 1032
Size: 970 360 3247
Fragments arranged by size:
3247 970 360
RsrII CG'GwC_CG
Cuts at: 1020 2772 1020
Size: 1752 2825
SacI G_AGCT'C
Cuts at: 1591 1591
Size: 4577
SalI G'TCGA_C
Cuts at: 1614 1614
Size: 4577
SapI GCTCTTCn'nnn_
Cuts at: 2600 2810 2600
Size: 210 4367
SbfI CC_TGCA'GG
Cuts at: 1624 1624
Size: 4577
ScaI AGT'ACT
Cuts at: 4094 4094
Size: 4577
SfcI C'TryA_G
Cuts at: 33 547 656 754 964 1620 2305 3298 3489
Size: 514 109 98 210 656 685 993 191
Cuts at: 3489 33
Size: 1121
Fragments arranged by size:
1121 993 685 656 514 210 191 109
98
SfoI GGC'GCC
Cuts at: 407 543 1347 1485 2009 2257 407
Size: 136 804 138 524 248 2727
Fragments arranged by size:
2727 804 524 248 138 136
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4577
SgrAI Cr'CCGG_yG
Cuts at: 262 262
Size: 4577
SmaI CCC'GGG
Cuts at: 535 1599 535
Size: 1064 3513
SmlI C'TyrA_G
Cuts at: 1732 2647 3139 3401 1732
Size: 915 492 262 2908
Fragments arranged by size:
2908 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4577
SphI G_CATG'C
Cuts at: 1630 2130 1630
Size: 500 4077
StuI AGG'CCT
Cuts at: 752 977 752
Size: 225 4352
StyI C'CwwG_G
Cuts at: 206 1109 1470 1551 1711 2065 206
Size: 903 361 81 160 354 2718
Fragments arranged by size:
2718 903 361 354 160 81
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1037 2160 2293 2752 2961 1037
Size: 1123 133 459 209 2653
Fragments arranged by size:
2653 1123 459 209 133
TatI w'GTAC_w
Cuts at: 4092 4092
Size: 4577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 4094 4126 4094
Size: 32 4545
Tth111I GACn'n_nGTC
Cuts at: 2374 2374
Size: 4577
XbaI T'CTAG_A
Cuts at: 786 1608 786
Size: 822 3755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 399 1558 2072 399
Size: 1159 514 2904
Fragments arranged by size:
2904 1159 514
XmaI C'CCGG_G
Cuts at: 533 1597 533
Size: 1064 3513
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PflMI PmeI PpiI
PpuMI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SpeI SphI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AflII AlfI ApaI AscI AvrII BaeI
BbsI BbvCI BfrBI BmgBI BmtI Bpu10I BsiWI BsmI
BsmBI BsrGI BstEII BstZ17I Bsu36I BtgI ClaI CspCI
DraIII EcoNI EcoRV FspAI HindIII HpaI MfeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PfoI
PmlI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SnaBI SrfI SspI SwaI XhoI XmnI
ZraI