Restriction map for
pF1KB7737
(Circular) (Six-base) MAPSORT of: pF1KB7737.seq Check: 7534 from: 1 to: 4421
pF1KB7737 4421 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 623 1459 623
Size: 836 3585
Acc65I G'GTAC_C
Cuts at: 1298 1320 1437 1298
Size: 22 117 4282
Fragments arranged by size:
4282 117 22
AclI AA'CG_TT
Cuts at: 4261 4261
Size: 4421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 907 2246 2678 3425 3693 907
Size: 1339 432 747 268 1635
Fragments arranged by size:
1635 1339 747 432 268
AflIII A'CryG_T
Cuts at: 1773 2877 3854 1773
Size: 1104 977 2340
Fragments arranged by size:
2340 1104 977
AgeI A'CCGG_T
Cuts at: 2809 2809
Size: 4421
AleI CACnn'nnGTG
Cuts at: 1125 1125
Size: 4421
AlwNI CAG_nnn'CTG
Cuts at: 493 938 1726 3293 493
Size: 445 788 1567 1621
Fragments arranged by size:
1621 1567 788 445
ApaI G_GGCC'C
Cuts at: 164 985 1185 1354 164
Size: 821 200 169 3231
Fragments arranged by size:
3231 821 200 169
ApaLI G'TGCA_C
Cuts at: 653 3191 653
Size: 2538 1883
ApoI r'AATT_y
Cuts at: 1425 3774 4030 1425
Size: 2349 256 1816
Fragments arranged by size:
2349 1816 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4421
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
AvaI C'yCGr_G
Cuts at: 372 505 832 1096 1441 372
Size: 133 327 264 345 3352
Fragments arranged by size:
3352 345 327 264 133
BamHI G'GATC_C
Cuts at: 1446 1446
Size: 4421
BanI G'GyrC_C
Cuts at: 1119 1298 1320 1437 1851 2099 2134 1119
Size: 179 22 117 414 248 35 3406
Fragments arranged by size:
3406 414 248 179 117 35 22
BanII G_rGCy'C
Cuts at: 164 555 587 605 985 1185 1210 1294 1354
Size: 391 32 18 380 200 25 84 60
Cuts at: 1354 1402 1435 2465 164
Size: 48 33 1030 2120
Fragments arranged by size:
2120 1030 391 380 200 84 60 48
33 32 25 18
BbeI G_GCGC'C
Cuts at: 1855 2103 1855
Size: 248 4173
BbvCI CC'TCA_GC
Cuts at: 1145 1145
Size: 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 625 659 625
Size: 34 4387
BciVI GTATCCnnnnn_n'
Cuts at: 2319 3080 4421 2319
Size: 761 1341 2319
Fragments arranged by size:
2319 1341 761
BclI T'GATC_A
Cuts at: 440 1941 440
Size: 1501 2920
BglI GCCn_nnn'nGGC
Cuts at: 1003 1003
Size: 4421
BglII A'GATC_T
Cuts at: 1936 3973 1936
Size: 2037 2384
BlpI GC'TnA_GC
Cuts at: 1242 1533 1242
Size: 291 4130
Bme1580I G_kGCm'C
Cuts at: 164 331 657 985 1185 1354 2046 2139 3195
Size: 167 326 328 200 169 692 93 1056
Cuts at: 3195 164
Size: 1390
Fragments arranged by size:
1390 1056 692 328 326 200 169 167
93
BmrI ACTGGGnnnn_n'
Cuts at: 813 1133 1181 1633 1714 2049 4193 813
Size: 320 48 452 81 335 2144 1041
Fragments arranged by size:
2144 1041 452 335 320 81 48
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 438 2788 438
Size: 2350 2071
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 335 412 1211 1288 168
Size: 167 77 799 77 3301
Fragments arranged by size:
3301 799 167 77 77
Bpu10I CC'TnA_GC
Cuts at: 385 1145 385
Size: 760 3661
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1597 2476 2968 3266 1597
Size: 879 492 298 2752
Fragments arranged by size:
2752 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 829 4125 829
Size: 3296 1125
BsaAI yAC'GTr
Cuts at: 1774 1774
Size: 4421
BsaBI GATnn'nnATC
Cuts at: 6 1935 3972 6
Size: 1929 2037 455
Fragments arranged by size:
2037 1929 455
BsaHI Gr'CG_yC
Cuts at: 956 1852 2100 2802 4298 956
Size: 896 248 702 1496 1079
Fragments arranged by size:
1496 1079 896 702 248
BsaWI w'CCGG_w
Cuts at: 540 1610 2131 2809 3083 3230 3641 540
Size: 1070 521 678 274 147 411 1320
Fragments arranged by size:
1320 1070 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1325 1355 1325
Size: 30 4391
BseYI C'CCAG_C
Cuts at: 454 578 586 785 1089 1210 1302 1846 2604
Size: 124 8 199 304 121 92 544 758
Cuts at: 2604 3181 3677 454
Size: 577 496 1198
Fragments arranged by size:
1198 758 577 544 496 304 199 124
121 92 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1144 721
Size: 423 3998
BsiEI CG_ry'CG
Cuts at: 104 1963 2009 3217 104
Size: 1859 46 1208 1308
Fragments arranged by size:
1859 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 657 1435 2213 2403 3195 657
Size: 778 778 190 792 1883
Fragments arranged by size:
1883 792 778 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 947 947
Size: 4421
Bsp1286I G_dGCh'C
Cuts at: 164 331 555 587 605 657 985 1185 1210
Size: 167 224 32 18 52 328 200 25
Cuts at: 1210 1294 1354 1402 1435 2046 2139 2213 2403
Size: 84 60 48 33 611 93 74 190
Cuts at: 2403 2465 3195 164
Size: 62 730 1390
Fragments arranged by size:
1390 730 611 328 224 200 190 167
93 84 74 62 60 52 48 33
32 25 18
BspEI T'CCGG_A
Cuts at: 3641 3641
Size: 4421
BspHI T'CATG_A
Cuts at: 3597 3597
Size: 4421
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1471 1685 1987 2368 1471
Size: 214 302 381 3524
Fragments arranged by size:
3524 381 302 214
BsrBI CCG'CTC
Cuts at: 50 1016 2713 2767 4251 4416 50
Size: 966 1697 54 1484 165 55
Fragments arranged by size:
1697 1484 966 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2333 2333
Size: 4421
BsrFI r'CCGG_y
Cuts at: 145 336 572 1314 2419 2600 2809 145
Size: 191 236 742 1105 181 209 1757
Fragments arranged by size:
1757 1105 742 236 209 191 181
BssSI C'ACGA_G
Cuts at: 119 2692 3050 119
Size: 2573 358 1490
Fragments arranged by size:
2573 1490 358
BstAPI GCAn_nnn'nTGC
Cuts at: 744 1798 744
Size: 1054 3367
BstBI TT'CG_AA
Cuts at: 2782 2782
Size: 4421
BstYI r'GATC_y
Cuts at: 1 1074 1446 1913 1936 2271 2517 3529 3615
Size: 1073 372 467 23 335 246 1012 86
Cuts at: 3615 3627 3973 1
Size: 12 346 449
Fragments arranged by size:
1073 1012 467 449 372 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 323 720 1176 323
Size: 397 456 3568
Fragments arranged by size:
3568 456 397
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2371 2552 2715 2371
Size: 181 163 4077
Fragments arranged by size:
4077 181 163
BtsI GCAGTG_nn'
Cuts at: 1664 1664
Size: 4421
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 107 142 107
Size: 35 4386
DraI TTT'AAA
Cuts at: 1420 1420
Size: 4421
DraIII CAC_nnn'GTG
Cuts at: 1030 1030
Size: 4421
DrdI GACnn_nn'nnGTC
Cuts at: 2127 2985 2127
Size: 858 3563
EaeI y'GGCC_r
Cuts at: 287 723 2006 2180 2571 2598 3943 287
Size: 436 1283 174 391 27 1345 765
Fragments arranged by size:
1345 1283 765 436 391 174 27
EagI C'GGCC_G
Cuts at: 2006 2006
Size: 4421
EarI CTCTTCn'nnn_
Cuts at: 961 2444 2654 961
Size: 1483 210 2728
Fragments arranged by size:
2728 1483 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2629 2939 3085 4231 2629
Size: 310 146 1146 2819
Fragments arranged by size:
2819 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1433 1433
Size: 4421
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 168 335 412 907 1211 1288 2246 2678 3425
Size: 167 77 495 304 77 958 432 747
Cuts at: 3425 3693 168
Size: 268 896
Fragments arranged by size:
958 896 747 495 432 304 268 167
77 77
EcoNI CCTnn'n_nnAGG
Cuts at: 205 205
Size: 4421
EcoO109I rG'GnC_Cy
Cuts at: 160 357 981 1086 1181 1182 1350 1351 1560
Size: 197 624 105 95 1 168 1 209
Cuts at: 1560 160
Size: 3021
Fragments arranged by size:
3021 624 209 197 168 105 95 1
1
EcoRI G'AATT_C
Cuts at: 1425 1425
Size: 4421
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2440 2440
Size: 4421
FspI TGC'GCA
Cuts at: 2202 2202
Size: 4421
HaeII r_GCGC'y
Cuts at: 579 1242 1855 2103 3125 4104 579
Size: 663 613 248 1022 979 896
Fragments arranged by size:
1022 979 896 663 613 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 473 505 1384 1416 2263 2293 2295 2325 473
Size: 32 879 32 847 30 2 30 2569
Fragments arranged by size:
2569 879 847 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1460 1460
Size: 4421
KasI G'GCGC_C
Cuts at: 1851 2099 1851
Size: 248 4173
KpnI G_GTAC'C
Cuts at: 1302 1324 1441 1302
Size: 22 117 4282
Fragments arranged by size:
4282 117 22
MluI A'CGCG_T
Cuts at: 3854 3854
Size: 4421
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3092 3276 3092
Size: 184 4237
MscI TGG'CCA
Cuts at: 2182 2182
Size: 4421
MslI CAynn'nnrTG
Cuts at: 850 1125 2410 2537 850
Size: 275 1285 127 2734
Fragments arranged by size:
2734 1285 275 127
MspA1I CmG'CkG
Cuts at: 563 590 1111 1306 1532 1846 2206 3219 3464
Size: 27 521 195 226 314 360 1013 245
Cuts at: 3464 563
Size: 1520
Fragments arranged by size:
1520 1013 521 360 314 245 226 195
27
NaeI GCC'GGC
Cuts at: 147 338 574 2602 147
Size: 191 236 2028 1966
Fragments arranged by size:
2028 1966 236 191
NarI GG'CG_CC
Cuts at: 1852 2100 1852
Size: 248 4173
NcoI C'CATG_G
Cuts at: 323 1176 323
Size: 853 3568
NgoMIV G'CCGG_C
Cuts at: 145 336 572 2600 145
Size: 191 236 2028 1966
Fragments arranged by size:
2028 1966 236 191
NspI r_CATG'y
Cuts at: 468 1474 1974 2881 468
Size: 1006 500 907 2008
Fragments arranged by size:
2008 1006 907 500
PasI CC'CwG_GG
Cuts at: 138 1050 138
Size: 912 3509
PciI A'CATG_T
Cuts at: 2877 2877
Size: 4421
PfoI T'CCnGG_A
Cuts at: 259 920 1217 259
Size: 661 297 3463
Fragments arranged by size:
3463 661 297
PmeI GTTT'AAAC
Cuts at: 1420 1420
Size: 4421
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3560 3592 3560
Size: 32 4389
PspOMI G'GGCC_C
Cuts at: 160 981 1181 1350 160
Size: 821 200 169 3231
Fragments arranged by size:
3231 821 200 169
PstI C_TGCA'G
Cuts at: 796 1468 2153 796
Size: 672 685 3064
Fragments arranged by size:
3064 685 672
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4421
PvuII CAG'CTG
Cuts at: 1846 2206 1846
Size: 360 4061
RsrII CG'GwC_CG
Cuts at: 2616 2616
Size: 4421
SacI G_AGCT'C
Cuts at: 1435 1435
Size: 4421
SalI G'TCGA_C
Cuts at: 1458 1458
Size: 4421
SapI GCTCTTCn'nnn_
Cuts at: 2444 2654 2444
Size: 210 4211
SbfI CC_TGCA'GG
Cuts at: 1468 1468
Size: 4421
ScaI AGT'ACT
Cuts at: 3938 3938
Size: 4421
SexAI A'CCwGG_T
Cuts at: 1026 1026
Size: 4421
SfcI C'TryA_G
Cuts at: 33 792 1464 2149 3142 3333 33
Size: 759 672 685 993 191 1121
Fragments arranged by size:
1121 993 759 685 672 191
SfoI GGC'GCC
Cuts at: 1853 2101 1853
Size: 248 4173
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4421
SgrAI Cr'CCGG_yG
Cuts at: 1314 1314
Size: 4421
SmaI CCC'GGG
Cuts at: 374 1098 1443 374
Size: 724 345 3352
Fragments arranged by size:
3352 724 345
SmlI C'TyrA_G
Cuts at: 1576 2491 2983 3245 1576
Size: 915 492 262 2752
Fragments arranged by size:
2752 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4421
SphI G_CATG'C
Cuts at: 1474 1974 1474
Size: 500 3921
StuI AGG'CCT
Cuts at: 1006 1150 1006
Size: 144 4277
StyI C'CwwG_G
Cuts at: 323 1176 1344 1555 1909 323
Size: 853 168 211 354 2835
Fragments arranged by size:
2835 853 354 211 168
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 311 1042 2004 2137 2596 2805 311
Size: 731 962 133 459 209 1927
Fragments arranged by size:
1927 962 731 459 209 133
TatI w'GTAC_w
Cuts at: 3936 3936
Size: 4421
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1325 1357 3938 3970 1325
Size: 32 2581 32 1776
Fragments arranged by size:
2581 1776 32 32
Tth111I GACn'n_nGTC
Cuts at: 619 2218 619
Size: 1599 2822
XbaI T'CTAG_A
Cuts at: 1452 1452
Size: 4421
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 708 1916 708
Size: 1208 3213
XmaI C'CCGG_G
Cuts at: 372 1096 1441 372
Size: 724 345 3352
Fragments arranged by size:
3352 724 345
XmnI GAAnn'nnTTC
Cuts at: 415 415
Size: 4421
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PfoI PmeI PpiI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SgrAI SmaI SmlI SpeI SphI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI AscI
AvrII BaeI BbsI BfrBI BmgBI BmtI BseRI BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PmlI PpuMI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SnaBI SrfI
SspI SwaI XhoI ZraI