Restriction map for
pF1KB7736
(Circular) (Six-base) MAPSORT of: pF1KB7736.seq Check: 5676 from: 1 to: 4559
pF1KB7736 4559 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 445 1597 445
Size: 1152 3407
Acc65I G'GTAC_C
Cuts at: 1575 1575
Size: 4559
AclI AA'CG_TT
Cuts at: 392 1028 4399 392
Size: 636 3371 552
Fragments arranged by size:
3371 636 552
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1397 2384 2816 3563 3831 1397
Size: 987 432 747 268 2125
Fragments arranged by size:
2125 987 747 432 268
AflIII A'CryG_T
Cuts at: 356 1911 3015 3992 356
Size: 1555 1104 977 923
Fragments arranged by size:
1555 1104 977 923
AgeI A'CCGG_T
Cuts at: 2947 2947
Size: 4559
AleI CACnn'nnGTG
Cuts at: 644 644
Size: 4559
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 394 394
Size: 4559
AlwNI CAG_nnn'CTG
Cuts at: 730 1203 1864 3431 730
Size: 473 661 1567 1858
Fragments arranged by size:
1858 1567 661 473
ApaLI G'TGCA_C
Cuts at: 3329 3329
Size: 4559
ApoI r'AATT_y
Cuts at: 328 964 1563 3912 4168 328
Size: 636 599 2349 256 719
Fragments arranged by size:
2349 719 636 599 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4559
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
AvaI C'yCGr_G
Cuts at: 1579 1579
Size: 4559
BamHI G'GATC_C
Cuts at: 1584 1584
Size: 4559
BanI G'GyrC_C
Cuts at: 124 1575 1989 2237 2272 124
Size: 1451 414 248 35 2411
Fragments arranged by size:
2411 1451 414 248 35
BanII G_rGCy'C
Cuts at: 1573 2603 1573
Size: 1030 3529
BbeI G_GCGC'C
Cuts at: 1993 2241 1993
Size: 248 4311
BbsI GAAGACnn'nnnn_
Cuts at: 1307 1307
Size: 4559
BbvCI CC'TCA_GC
Cuts at: 290 290
Size: 4559
BciVI GTATCCnnnnn_n'
Cuts at: 1062 2457 3218 4559 1062
Size: 1395 761 1341 1062
Fragments arranged by size:
1395 1341 1062 761
BclI T'GATC_A
Cuts at: 2079 2079
Size: 4559
BglII A'GATC_T
Cuts at: 2074 4111 2074
Size: 2037 2522
BlpI GC'TnA_GC
Cuts at: 1671 1671
Size: 4559
Bme1580I G_kGCm'C
Cuts at: 127 2184 2277 3333 127
Size: 2057 93 1056 1353
Fragments arranged by size:
2057 1353 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 740 1771 1852 2187 4331 740
Size: 1031 81 335 2144 968
Fragments arranged by size:
2144 1031 968 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2926 2926
Size: 4559
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1204
Size: 4559
Bpu10I CC'TnA_GC
Cuts at: 290 290
Size: 4559
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1735 2614 3106 3404 1735
Size: 879 492 298 2890
Fragments arranged by size:
2890 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 250 4263 250
Size: 4013 546
BsaAI yAC'GTr
Cuts at: 1912 1912
Size: 4559
BsaBI GATnn'nnATC
Cuts at: 6 2073 4110 6
Size: 2067 2037 455
Fragments arranged by size:
2067 2037 455
BsaHI Gr'CG_yC
Cuts at: 1268 1990 2238 2940 4436 1268
Size: 722 248 702 1496 1391
Fragments arranged by size:
1496 1391 722 702 248
BsaWI w'CCGG_w
Cuts at: 1748 2269 2947 3221 3368 3779 1748
Size: 521 678 274 147 411 2528
Fragments arranged by size:
2528 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1531 1561 1531
Size: 30 4529
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 1294 1323 1396 1483 1507 1522 1552 307
Size: 987 29 73 87 24 15 30 3314
Fragments arranged by size:
3314 987 87 73 30 29 24 15
BseYI C'CCAG_C
Cuts at: 514 999 1984 2742 3319 3815 514
Size: 485 985 758 577 496 1258
Fragments arranged by size:
1258 985 758 577 496 485
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 508
Size: 4559
BsiEI CG_ry'CG
Cuts at: 104 2101 2147 3355 104
Size: 1997 46 1208 1308
Fragments arranged by size:
1997 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1573 2351 2541 3333 1573
Size: 778 190 792 2799
Fragments arranged by size:
2799 792 778 190
BsmI GAATG_Cn'
Cuts at: 1226 1226
Size: 4559
BsmBI CGTCTCn'nnnn_
Cuts at: 788 788
Size: 4559
Bsp1286I G_dGCh'C
Cuts at: 127 1573 2184 2277 2351 2541 2603 3333 127
Size: 1446 611 93 74 190 62 730 1353
Fragments arranged by size:
1446 1353 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3779 3779
Size: 4559
BspHI T'CATG_A
Cuts at: 686 3735 686
Size: 3049 1510
BspMI ACCTGCnnnn'nnnn_
Cuts at: 992 1609 1823 2125 2506 992
Size: 617 214 302 381 3045
Fragments arranged by size:
3045 617 381 302 214
BsrBI CCG'CTC
Cuts at: 50 789 2851 2905 4389 4554 50
Size: 739 2062 54 1484 165 55
Fragments arranged by size:
2062 1484 739 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2471 2471
Size: 4559
BsrFI r'CCGG_y
Cuts at: 834 2557 2738 2947 834
Size: 1723 181 209 2446
Fragments arranged by size:
2446 1723 209 181
BsrGI T'GTAC_A
Cuts at: 620 620
Size: 4559
BssSI C'ACGA_G
Cuts at: 2830 3188 2830
Size: 358 4201
BstAPI GCAn_nnn'nTGC
Cuts at: 1936 1936
Size: 4559
BstBI TT'CG_AA
Cuts at: 2920 2920
Size: 4559
BstXI CCAn_nnnn'nTGG
Cuts at: 852 960 852
Size: 108 4451
BstYI r'GATC_y
Cuts at: 1 1076 1584 2051 2074 2409 2655 3667 3753
Size: 1075 508 467 23 335 246 1012 86
Cuts at: 3753 3765 4111 1
Size: 12 346 449
Fragments arranged by size:
1075 1012 508 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1262 1277 1262
Size: 15 4544
BtgI C'CryG_G
Cuts at: 107 642 674 107
Size: 535 32 3992
Fragments arranged by size:
3992 535 32
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 329 483 1304 2509 2690 2853 329
Size: 154 821 1205 181 163 2035
Fragments arranged by size:
2035 1205 821 181 163 154
BtsI GCAGTG_nn'
Cuts at: 1802 1802
Size: 4559
DraI TTT'AAA
Cuts at: 1558 1558
Size: 4559
DrdI GACnn_nn'nnGTC
Cuts at: 2265 3123 2265
Size: 858 3701
EaeI y'GGCC_r
Cuts at: 581 1550 2144 2318 2709 2736 4081 581
Size: 969 594 174 391 27 1345 1059
Fragments arranged by size:
1345 1059 969 594 391 174 27
EagI C'GGCC_G
Cuts at: 2144 2144
Size: 4559
EarI CTCTTCn'nnn_
Cuts at: 653 1355 1373 1413 1484 2582 2792 653
Size: 702 18 40 71 1098 210 2420
Fragments arranged by size:
2420 1098 702 210 71 40 18
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2767 3077 3223 4369 2767
Size: 310 146 1146 2957
Fragments arranged by size:
2957 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1571 1571
Size: 4559
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1204 1397 2384 2816 3563 3831 1204
Size: 193 987 432 747 268 1932
Fragments arranged by size:
1932 987 747 432 268 193
EcoO109I rG'GnC_Cy
Cuts at: 519 735 1698 519
Size: 216 963 3380
Fragments arranged by size:
3380 963 216
EcoRI G'AATT_C
Cuts at: 1563 1563
Size: 4559
EcoRV GAT'ATC
Cuts at: 573 747 573
Size: 174 4385
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2578 2578
Size: 4559
FspI TGC'GCA
Cuts at: 2340 2340
Size: 4559
HaeII r_GCGC'y
Cuts at: 1993 2241 3263 4242 1993
Size: 248 1022 979 2310
Fragments arranged by size:
2310 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 343 375 796 828 889 921 2401 2431 2433
Size: 32 421 32 61 32 1480 30 2
Cuts at: 2433 2463 343
Size: 30 2439
Fragments arranged by size:
2439 1480 421 61 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 780 1598 780
Size: 818 3741
HpaI GTT'AAC
Cuts at: 780 780
Size: 4559
KasI G'GCGC_C
Cuts at: 1989 2237 1989
Size: 248 4311
KpnI G_GTAC'C
Cuts at: 1579 1579
Size: 4559
MluI A'CGCG_T
Cuts at: 3992 3992
Size: 4559
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 144 341 1436 3230 3414 144
Size: 197 1095 1794 184 1289
Fragments arranged by size:
1794 1289 1095 197 184
MscI TGG'CCA
Cuts at: 583 2320 583
Size: 1737 2822
MslI CAynn'nnrTG
Cuts at: 314 502 644 691 707 2548 2675 314
Size: 188 142 47 16 1841 127 2198
Fragments arranged by size:
2198 1841 188 142 127 47 16
MspA1I CmG'CkG
Cuts at: 1057 1670 1984 2344 3357 3602 1057
Size: 613 314 360 1013 245 2014
Fragments arranged by size:
2014 1013 613 360 314 245
NaeI GCC'GGC
Cuts at: 2740 2740
Size: 4559
NarI GG'CG_CC
Cuts at: 1990 2238 1990
Size: 248 4311
NcoI C'CATG_G
Cuts at: 107 642 107
Size: 535 4024
NdeI CA'TA_TG
Cuts at: 1047 1047
Size: 4559
NgoMIV G'CCGG_C
Cuts at: 2738 2738
Size: 4559
NspI r_CATG'y
Cuts at: 360 1612 2112 3019 360
Size: 1252 500 907 1900
Fragments arranged by size:
1900 1252 907 500
PciI A'CATG_T
Cuts at: 356 3015 356
Size: 2659 1900
PflMI CCAn_nnn'nTGG
Cuts at: 730 730
Size: 4559
PfoI T'CCnGG_A
Cuts at: 587 587
Size: 4559
PmeI GTTT'AAAC
Cuts at: 1558 1558
Size: 4559
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1348 1380 3698 3730 1348
Size: 32 2318 32 2177
Fragments arranged by size:
2318 2177 32 32
PpuMI rG'GwC_Cy
Cuts at: 735 735
Size: 4559
PstI C_TGCA'G
Cuts at: 1606 2291 1606
Size: 685 3874
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4559
PvuII CAG'CTG
Cuts at: 1057 1984 2344 1057
Size: 927 360 3272
Fragments arranged by size:
3272 927 360
RsrII CG'GwC_CG
Cuts at: 678 2754 678
Size: 2076 2483
SacI G_AGCT'C
Cuts at: 1573 1573
Size: 4559
SalI G'TCGA_C
Cuts at: 1596 1596
Size: 4559
SapI GCTCTTCn'nnn_
Cuts at: 2582 2792 2582
Size: 210 4349
SbfI CC_TGCA'GG
Cuts at: 1606 1606
Size: 4559
ScaI AGT'ACT
Cuts at: 718 4076 718
Size: 3358 1201
SfcI C'TryA_G
Cuts at: 33 1602 2287 3280 3471 33
Size: 1569 685 993 191 1121
Fragments arranged by size:
1569 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1991 2239 1991
Size: 248 4311
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4559
SmaI CCC'GGG
Cuts at: 1581 1581
Size: 4559
SmlI C'TyrA_G
Cuts at: 1714 2629 3121 3383 1714
Size: 915 492 262 2890
Fragments arranged by size:
2890 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4559
SphI G_CATG'C
Cuts at: 1612 2112 1612
Size: 500 4059
StuI AGG'CCT
Cuts at: 664 664
Size: 4559
StyI C'CwwG_G
Cuts at: 107 642 1502 1693 2047 107
Size: 535 860 191 354 2619
Fragments arranged by size:
2619 860 535 354 191
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 206 2142 2275 2734 2943 206
Size: 1936 133 459 209 1822
Fragments arranged by size:
1936 1822 459 209 133
TatI w'GTAC_w
Cuts at: 365 620 716 4074 365
Size: 255 96 3358 850
Fragments arranged by size:
3358 850 255 96
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1226 1258 4076 4108 1226
Size: 32 2818 32 1677
Fragments arranged by size:
2818 1677 32 32
Tth111I GACn'n_nGTC
Cuts at: 682 693 2356 682
Size: 11 1663 2885
Fragments arranged by size:
2885 1663 11
XbaI T'CTAG_A
Cuts at: 1590 1590
Size: 4559
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2054 2054
Size: 4559
XmaI C'CCGG_G
Cuts at: 1579 1579
Size: 4559
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI
FspI HaeII Hin4I HincII HpaI KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NspI PciI PflMI PfoI PmeI PpiI PpuMI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI FseI
FspAI HindIII MfeI NheI NotI NruI NsiI PacI
PasI PmlI PshAI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI