Restriction map for
pF1KB7721
(Circular) (Six-base) MAPSORT of: pF1KB7721.seq Check: 9530 from: 1 to: 4397
pF1KB7721 4397 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AccI GT'mk_AC
Cuts at: 1435 1435
Size: 4397
Acc65I G'GTAC_C
Cuts at: 1413 1413
Size: 4397
AclI AA'CG_TT
Cuts at: 4237 4237
Size: 4397
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1225 2222 2654 3401 3669 1225
Size: 997 432 747 268 1953
Fragments arranged by size:
1953 997 747 432 268
AflIII A'CryG_T
Cuts at: 278 722 1749 2853 3830 278
Size: 444 1027 1104 977 845
Fragments arranged by size:
1104 1027 977 845 444
AgeI A'CCGG_T
Cuts at: 2785 2785
Size: 4397
AleI CACnn'nnGTG
Cuts at: 626 626
Size: 4397
AlwNI CAG_nnn'CTG
Cuts at: 1170 1702 3269 1170
Size: 532 1567 2298
Fragments arranged by size:
2298 1567 532
ApaLI G'TGCA_C
Cuts at: 3167 3167
Size: 4397
ApoI r'AATT_y
Cuts at: 1401 3750 4006 1401
Size: 2349 256 1792
Fragments arranged by size:
2349 1792 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4397
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
AvaI C'yCGr_G
Cuts at: 400 593 1417 400
Size: 193 824 3380
Fragments arranged by size:
3380 824 193
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 1150 1183 1150
Size: 33 4364
BamHI G'GATC_C
Cuts at: 1422 1422
Size: 4397
BanI G'GyrC_C
Cuts at: 1332 1413 1827 2075 2110 1332
Size: 81 414 248 35 3619
Fragments arranged by size:
3619 414 248 81 35
BanII G_rGCy'C
Cuts at: 407 1411 2441 407
Size: 1004 1030 2363
Fragments arranged by size:
2363 1030 1004
BbeI G_GCGC'C
Cuts at: 1831 2079 1831
Size: 248 4149
BbsI GAAGACnn'nnnn_
Cuts at: 240 240
Size: 4397
BbvCI CC'TCA_GC
Cuts at: 919 919
Size: 4397
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 890 924 1084 1118 1125 1159 890
Size: 34 160 34 7 34 4128
Fragments arranged by size:
4128 160 34 34 34 7
BciVI GTATCCnnnnn_n'
Cuts at: 2295 3056 4397 2295
Size: 761 1341 2295
Fragments arranged by size:
2295 1341 761
BclI T'GATC_A
Cuts at: 1917 1917
Size: 4397
BglII A'GATC_T
Cuts at: 470 1912 3949 470
Size: 1442 2037 918
Fragments arranged by size:
2037 1442 918
BlpI GC'TnA_GC
Cuts at: 1509 1509
Size: 4397
Bme1580I G_kGCm'C
Cuts at: 773 2022 2115 3171 773
Size: 1249 93 1056 1999
Fragments arranged by size:
1999 1249 1056 93
BmgBI CAC'GTC
Cuts at: 144 1245 144
Size: 1101 3296
BmrI ACTGGGnnnn_n'
Cuts at: 385 501 1609 1690 2025 4169 385
Size: 116 1108 81 335 2144 613
Fragments arranged by size:
2144 1108 613 335 116 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 435 2764 435
Size: 2329 2068
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 853 592
Size: 261 4136
Bpu10I CC'TnA_GC
Cuts at: 919 919
Size: 4397
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1573 2452 2944 3242 565
Size: 1008 879 492 298 1720
Fragments arranged by size:
1720 1008 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 232 1349 4101 232
Size: 1117 2752 528
Fragments arranged by size:
2752 1117 528
BsaAI yAC'GTr
Cuts at: 857 1750 857
Size: 893 3504
BsaBI GATnn'nnATC
Cuts at: 6 565 1911 3948 6
Size: 559 1346 2037 455
Fragments arranged by size:
2037 1346 559 455
BsaHI Gr'CG_yC
Cuts at: 1828 2076 2778 4274 1828
Size: 248 702 1496 1951
Fragments arranged by size:
1951 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1586 2107 2785 3059 3206 3617 1586
Size: 521 678 274 147 411 2366
Fragments arranged by size:
2366 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 250 280 250
Size: 30 4367
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1084 1309 1324 1084
Size: 225 15 4157
Fragments arranged by size:
4157 225 15
BseYI C'CCAG_C
Cuts at: 455 984 1822 2580 3157 3653 455
Size: 529 838 758 577 496 1199
Fragments arranged by size:
1199 838 758 577 529 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 490 490
Size: 4397
BsiEI CG_ry'CG
Cuts at: 104 1939 1985 3193 104
Size: 1835 46 1208 1308
Fragments arranged by size:
1835 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 602 869 1411 2189 2379 3171 602
Size: 267 542 778 190 792 1828
Fragments arranged by size:
1828 792 778 542 267 190
BsmI GAATG_Cn'
Cuts at: 946 946
Size: 4397
Bsp1286I G_dGCh'C
Cuts at: 407 602 773 869 1411 2022 2115 2189 2379
Size: 195 171 96 542 611 93 74 190
Cuts at: 2379 2441 3171 407
Size: 62 730 1633
Fragments arranged by size:
1633 730 611 542 195 190 171 96
93 74 62
BspEI T'CCGG_A
Cuts at: 3617 3617
Size: 4397
BspHI T'CATG_A
Cuts at: 668 1239 3573 668
Size: 571 2334 1492
Fragments arranged by size:
2334 1492 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1447 1661 1963 2344 1447
Size: 214 302 381 3500
Fragments arranged by size:
3500 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2689 2743 4227 4392 50
Size: 2639 54 1484 165 55
Fragments arranged by size:
2639 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2309 2309
Size: 4397
BsrFI r'CCGG_y
Cuts at: 819 2395 2576 2785 819
Size: 1576 181 209 2431
Fragments arranged by size:
2431 1576 209 181
BssSI C'ACGA_G
Cuts at: 979 2668 3026 979
Size: 1689 358 2350
Fragments arranged by size:
2350 1689 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1774 1774
Size: 4397
BstBI TT'CG_AA
Cuts at: 2758 2758
Size: 4397
BstXI CCAn_nnnn'nTGG
Cuts at: 120 120
Size: 4397
BstYI r'GATC_y
Cuts at: 1 470 1178 1422 1889 1912 2247 2493 3505
Size: 469 708 244 467 23 335 246 1012
Cuts at: 3505 3591 3603 3949 1
Size: 86 12 346 449
Fragments arranged by size:
1012 708 469 467 449 346 335 246
244 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1307 1307
Size: 4397
BtgI C'CryG_G
Cuts at: 107 119 624 107
Size: 12 505 3880
Fragments arranged by size:
3880 505 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 172 395 1025 1394 2347 2528 2691 172
Size: 223 630 369 953 181 163 1878
Fragments arranged by size:
1878 953 630 369 223 181 163
BtsI GCAGTG_nn'
Cuts at: 1640 1640
Size: 4397
ClaI AT'CG_AT
Cuts at: 900 900
Size: 4397
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 843 878 843
Size: 35 4362
DraI TTT'AAA
Cuts at: 1396 1396
Size: 4397
DraIII CAC_nnn'GTG
Cuts at: 1228 1228
Size: 4397
DrdI GACnn_nn'nnGTC
Cuts at: 2103 2961 2103
Size: 858 3539
EaeI y'GGCC_r
Cuts at: 110 747 1982 2156 2547 2574 3919 110
Size: 637 1235 174 391 27 1345 588
Fragments arranged by size:
1345 1235 637 588 391 174 27
EagI C'GGCC_G
Cuts at: 1982 1982
Size: 4397
EarI CTCTTCn'nnn_
Cuts at: 1049 2420 2630 1049
Size: 1371 210 2816
Fragments arranged by size:
2816 1371 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 1171 2605 2915 3061 4207 1171
Size: 1434 310 146 1146 1361
Fragments arranged by size:
1434 1361 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1409 1409
Size: 4397
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 592 853 1225 2222 2654 3401 3669 592
Size: 261 372 997 432 747 268 1320
Fragments arranged by size:
1320 997 747 432 372 268 261
EcoNI CCTnn'n_nnAGG
Cuts at: 713 1269 713
Size: 556 3841
EcoO109I rG'GnC_Cy
Cuts at: 1303 1536 1303
Size: 233 4164
EcoRI G'AATT_C
Cuts at: 1401 1401
Size: 4397
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2416 2416
Size: 4397
FspI TGC'GCA
Cuts at: 2178 2178
Size: 4397
HaeII r_GCGC'y
Cuts at: 1831 2079 3101 4080 1831
Size: 248 1022 979 2148
Fragments arranged by size:
2148 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2239 2269 2271 2301 2239
Size: 30 2 30 4335
Fragments arranged by size:
4335 30 30 2
HincII GTy'rAC
Cuts at: 549 1436 549
Size: 887 3510
HindIII A'AGCT_T
Cuts at: 644 644
Size: 4397
KasI G'GCGC_C
Cuts at: 1827 2075 1827
Size: 248 4149
KpnI G_GTAC'C
Cuts at: 1417 1417
Size: 4397
MluI A'CGCG_T
Cuts at: 3830 3830
Size: 4397
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3068 3252 3068
Size: 184 4213
MscI TGG'CCA
Cuts at: 112 2158 112
Size: 2046 2351
MslI CAynn'nnrTG
Cuts at: 337 626 673 1127 1364 2386 2513 337
Size: 289 47 454 237 1022 127 2221
Fragments arranged by size:
2221 1022 454 289 237 127 47
MspA1I CmG'CkG
Cuts at: 459 1022 1508 1822 2182 3195 3440 459
Size: 563 486 314 360 1013 245 1416
Fragments arranged by size:
1416 1013 563 486 360 314 245
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 4397
NarI GG'CG_CC
Cuts at: 1828 2076 1828
Size: 248 4149
NcoI C'CATG_G
Cuts at: 107 624 107
Size: 517 3880
NdeI CA'TA_TG
Cuts at: 1032 1032
Size: 4397
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 4397
NspI r_CATG'y
Cuts at: 282 726 1450 1950 2857 282
Size: 444 724 500 907 1822
Fragments arranged by size:
1822 907 724 500 444
PciI A'CATG_T
Cuts at: 278 722 2853 278
Size: 444 2131 1822
Fragments arranged by size:
2131 1822 444
PflMI CCAn_nnn'nTGG
Cuts at: 192 210 491 837 1170 192
Size: 18 281 346 333 3419
Fragments arranged by size:
3419 346 333 281 18
PfoI T'CCnGG_A
Cuts at: 569 569
Size: 4397
PmeI GTTT'AAAC
Cuts at: 1396 1396
Size: 4397
PmlI CAC'GTG
Cuts at: 857 857
Size: 4397
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3536 3568 3536
Size: 32 4365
PpuMI rG'GwC_Cy
Cuts at: 1303 1303
Size: 4397
PstI C_TGCA'G
Cuts at: 320 1254 1444 2129 320
Size: 934 190 685 2588
Fragments arranged by size:
2588 934 685 190
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4397
PvuII CAG'CTG
Cuts at: 459 1822 2182 459
Size: 1363 360 2674
Fragments arranged by size:
2674 1363 360
RsrII CG'GwC_CG
Cuts at: 2592 2592
Size: 4397
SacI G_AGCT'C
Cuts at: 1411 1411
Size: 4397
SalI G'TCGA_C
Cuts at: 1434 1434
Size: 4397
SapI GCTCTTCn'nnn_
Cuts at: 2420 2630 2420
Size: 210 4187
SbfI CC_TGCA'GG
Cuts at: 1444 1444
Size: 4397
ScaI AGT'ACT
Cuts at: 1099 3914 1099
Size: 2815 1582
SexAI A'CCwGG_T
Cuts at: 830 830
Size: 4397
SfcI C'TryA_G
Cuts at: 33 316 1080 1250 1317 1440 2125 3118 3309
Size: 283 764 170 67 123 685 993 191
Cuts at: 3309 33
Size: 1121
Fragments arranged by size:
1121 993 764 685 283 191 170 123
67
SfoI GGC'GCC
Cuts at: 1829 2077 1829
Size: 248 4149
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4397
SmaI CCC'GGG
Cuts at: 402 1419 402
Size: 1017 3380
SmlI C'TyrA_G
Cuts at: 580 1552 2467 2959 3221 580
Size: 972 915 492 262 1756
Fragments arranged by size:
1756 972 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4397
SphI G_CATG'C
Cuts at: 1450 1950 1450
Size: 500 3897
StuI AGG'CCT
Cuts at: 265 529 265
Size: 264 4133
StyI C'CwwG_G
Cuts at: 107 624 1531 1885 107
Size: 517 907 354 2619
Fragments arranged by size:
2619 907 517 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 976 1350 1980 2113 2572 2781 976
Size: 374 630 133 459 209 2592
Fragments arranged by size:
2592 630 459 374 209 133
TatI w'GTAC_w
Cuts at: 1097 3912 1097
Size: 2815 1582
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1107 1139 1214 1246 3914 3946 1107
Size: 32 75 32 2668 32 1558
Fragments arranged by size:
2668 1558 75 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 664 675 2194 664
Size: 11 1519 2867
Fragments arranged by size:
2867 1519 11
XbaI T'CTAG_A
Cuts at: 1428 1428
Size: 4397
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 163 1892 163
Size: 1729 2668
XmaI C'CCGG_G
Cuts at: 400 1417 400
Size: 1017 3380
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlwNI
ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglII
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NdeI NgoMIV NspI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AlfI AloI ApaI
AscI AvrII BfrBI BglI BmtI BsiWI BsmBI BsrGI
BssHII BstEII BstZ17I EcoRV FseI FspAI HpaI MfeI
NheI NotI NruI NsiI PacI PasI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI XmnI ZraI