Restriction map for
pF1KB7719
(Circular) (Six-base) MAPSORT of: pF1KB7719.seq Check: 1563 from: 1 to: 4394
pF1KB7719 4394 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 292 1247 292
Size: 955 3439
AccI GT'mk_AC
Cuts at: 1432 1432
Size: 4394
Acc65I G'GTAC_C
Cuts at: 1410 1410
Size: 4394
AclI AA'CG_TT
Cuts at: 4234 4234
Size: 4394
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 234 451 671 916 1087 2219 2651 3398 3666
Size: 217 220 245 171 1132 432 747 268
Cuts at: 3666 234
Size: 962
Fragments arranged by size:
1132 962 747 432 268 245 220 217
171
AflIII A'CryG_T
Cuts at: 956 1746 2850 3827 956
Size: 790 1104 977 1523
Fragments arranged by size:
1523 1104 977 790
AgeI A'CCGG_T
Cuts at: 2782 2782
Size: 4394
AhdI GACnn_n'nnGTC
Cuts at: 957 1182 957
Size: 225 4169
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 151 1255 151
Size: 1104 3290
AlwNI CAG_nnn'CTG
Cuts at: 1699 3266 1699
Size: 1567 2827
ApaLI G'TGCA_C
Cuts at: 3164 3164
Size: 4394
ApoI r'AATT_y
Cuts at: 1398 3747 4003 1398
Size: 2349 256 1789
Fragments arranged by size:
2349 1789 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4394
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
AvaI C'yCGr_G
Cuts at: 1227 1414 1227
Size: 187 4207
BamHI G'GATC_C
Cuts at: 1419 1419
Size: 4394
BanI G'GyrC_C
Cuts at: 1410 1824 2072 2107 1410
Size: 414 248 35 3697
Fragments arranged by size:
3697 414 248 35
BanII G_rGCy'C
Cuts at: 1043 1408 2438 1043
Size: 365 1030 2999
Fragments arranged by size:
2999 1030 365
BbeI G_GCGC'C
Cuts at: 1828 2076 1828
Size: 248 4146
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 314 348 314
Size: 34 4360
BciVI GTATCCnnnnn_n'
Cuts at: 2292 3053 4394 2292
Size: 761 1341 2292
Fragments arranged by size:
2292 1341 761
BclI T'GATC_A
Cuts at: 1914 1914
Size: 4394
BglI GCCn_nnn'nGGC
Cuts at: 112 112
Size: 4394
BglII A'GATC_T
Cuts at: 1382 1909 3946 1382
Size: 527 2037 1830
Fragments arranged by size:
2037 1830 527
BlpI GC'TnA_GC
Cuts at: 1506 1506
Size: 4394
Bme1580I G_kGCm'C
Cuts at: 170 2019 2112 3168 170
Size: 1849 93 1056 1396
Fragments arranged by size:
1849 1396 1056 93
BmgBI CAC'GTC
Cuts at: 1005 1005
Size: 4394
BmrI ACTGGGnnnn_n'
Cuts at: 1606 1687 2022 4166 1606
Size: 81 335 2144 1834
Fragments arranged by size:
2144 1834 335 81
BmtI G_CTAG'C
Cuts at: 1270 1270
Size: 4394
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1314 2761 1314
Size: 1447 2947
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 557 606 1048 167
Size: 390 49 442 3513
Fragments arranged by size:
3513 442 390 49
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1570 2449 2941 3239 1570
Size: 879 492 298 2725
Fragments arranged by size:
2725 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 185 857 4098 185
Size: 672 3241 481
Fragments arranged by size:
3241 672 481
BsaAI yAC'GTr
Cuts at: 1747 1747
Size: 4394
BsaBI GATnn'nnATC
Cuts at: 6 1908 3945 6
Size: 1902 2037 455
Fragments arranged by size:
2037 1902 455
BsaHI Gr'CG_yC
Cuts at: 327 518 1151 1825 2073 2775 4271 327
Size: 191 633 674 248 702 1496 450
Fragments arranged by size:
1496 702 674 633 450 248 191
BsaWI w'CCGG_w
Cuts at: 158 1583 2104 2782 3056 3203 3614 158
Size: 1425 521 678 274 147 411 938
Fragments arranged by size:
1425 938 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 640 670 640
Size: 30 4364
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 457 460 499 642 645 648 1102 1105 1306
Size: 3 39 143 3 3 454 3 201
Cuts at: 1306 457
Size: 3545
Fragments arranged by size:
3545 454 201 143 39 3 3 3
3
BseYI C'CCAG_C
Cuts at: 634 773 1819 2577 3154 3650 634
Size: 139 1046 758 577 496 1378
Fragments arranged by size:
1378 1046 758 577 496 139
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 1165 1356 427
Size: 738 191 3465
Fragments arranged by size:
3465 738 191
BsiEI CG_ry'CG
Cuts at: 104 1936 1982 3190 104
Size: 1832 46 1208 1308
Fragments arranged by size:
1832 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1298 1364 1408 2186 2376 3168 1298
Size: 66 44 778 190 792 2524
Fragments arranged by size:
2524 792 778 190 66 44
BsmBI CGTCTCn'nnnn_
Cuts at: 1128 1128
Size: 4394
Bsp1286I G_dGCh'C
Cuts at: 170 1043 1298 1364 1408 2019 2112 2186 2376
Size: 873 255 66 44 611 93 74 190
Cuts at: 2376 2438 3168 170
Size: 62 730 1396
Fragments arranged by size:
1396 873 730 611 255 190 93 74
66 62 44
BspEI T'CCGG_A
Cuts at: 3614 3614
Size: 4394
BspHI T'CATG_A
Cuts at: 3570 3570
Size: 4394
BspMI ACCTGCnnnn'nnnn_
Cuts at: 292 1247 1283 1444 1658 1960 2341 292
Size: 955 36 161 214 302 381 2345
Fragments arranged by size:
2345 955 381 302 214 161 36
BsrBI CCG'CTC
Cuts at: 50 2686 2740 4224 4389 50
Size: 2636 54 1484 165 55
Fragments arranged by size:
2636 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 122 500 1383 2306 122
Size: 378 883 923 2210
Fragments arranged by size:
2210 923 883 378
BsrFI r'CCGG_y
Cuts at: 344 2392 2573 2782 344
Size: 2048 181 209 1956
Fragments arranged by size:
2048 1956 209 181
BssSI C'ACGA_G
Cuts at: 2665 3023 2665
Size: 358 4036
BstAPI GCAn_nnn'nTGC
Cuts at: 1101 1771 1101
Size: 670 3724
BstBI TT'CG_AA
Cuts at: 2755 2755
Size: 4394
BstXI CCAn_nnnn'nTGG
Cuts at: 513 513
Size: 4394
BstYI r'GATC_y
Cuts at: 1 173 723 1382 1419 1886 1909 2244 2490
Size: 172 550 659 37 467 23 335 246
Cuts at: 2490 3502 3588 3600 3946 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 659 550 467 449 346 335 246
172 86 37 23 12
BtgI C'CryG_G
Cuts at: 107 947 107
Size: 840 3554
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2344 2525 2688 2344
Size: 181 163 4050
Fragments arranged by size:
4050 181 163
BtsI GCAGTG_nn'
Cuts at: 330 1192 1637 330
Size: 862 445 3087
Fragments arranged by size:
3087 862 445
DraI TTT'AAA
Cuts at: 1393 1393
Size: 4394
DrdI GACnn_nn'nnGTC
Cuts at: 2100 2958 2100
Size: 858 3536
EaeI y'GGCC_r
Cuts at: 122 1109 1979 2153 2544 2571 3916 122
Size: 987 870 174 391 27 1345 600
Fragments arranged by size:
1345 987 870 600 391 174 27
EagI C'GGCC_G
Cuts at: 1979 1979
Size: 4394
EarI CTCTTCn'nnn_
Cuts at: 690 2417 2627 690
Size: 1727 210 2457
Fragments arranged by size:
2457 1727 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2602 2912 3058 4204 2602
Size: 310 146 1146 2792
Fragments arranged by size:
2792 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1406 1406
Size: 4394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 167 234 451 557 606 671 916 1048 1087
Size: 67 217 106 49 65 245 132 39
Cuts at: 1087 2219 2651 3398 3666 167
Size: 1132 432 747 268 895
Fragments arranged by size:
1132 895 747 432 268 245 217 132
106 67 65 49 39
EcoNI CCTnn'n_nnAGG
Cuts at: 1288 1288
Size: 4394
EcoO109I rG'GnC_Cy
Cuts at: 1533 1533
Size: 4394
EcoRI G'AATT_C
Cuts at: 1398 1398
Size: 4394
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 1095 2413 1095
Size: 1318 3076
FspI TGC'GCA
Cuts at: 1279 2175 1279
Size: 896 3498
HaeII r_GCGC'y
Cuts at: 1828 2076 3098 4077 1828
Size: 248 1022 979 2145
Fragments arranged by size:
2145 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 640 672 856 888 943 975 1168 1200 2236
Size: 32 184 32 55 32 193 32 1036
Cuts at: 2236 2266 2268 2298 640
Size: 30 2 30 2736
Fragments arranged by size:
2736 1036 193 184 55 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1433 1433
Size: 4394
KasI G'GCGC_C
Cuts at: 1824 2072 1824
Size: 248 4146
KpnI G_GTAC'C
Cuts at: 1414 1414
Size: 4394
MluI A'CGCG_T
Cuts at: 3827 3827
Size: 4394
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 575 1211 3065 3249 575
Size: 636 1854 184 1720
Fragments arranged by size:
1854 1720 636 184
MscI TGG'CCA
Cuts at: 1111 2155 1111
Size: 1044 3350
MslI CAynn'nnrTG
Cuts at: 215 370 2383 2510 215
Size: 155 2013 127 2099
Fragments arranged by size:
2099 2013 155 127
MspA1I CmG'CkG
Cuts at: 1211 1313 1505 1819 2179 3192 3437 1211
Size: 102 192 314 360 1013 245 2168
Fragments arranged by size:
2168 1013 360 314 245 192 102
NaeI GCC'GGC
Cuts at: 346 2575 346
Size: 2229 2165
NarI GG'CG_CC
Cuts at: 1825 2073 1825
Size: 248 4146
NcoI C'CATG_G
Cuts at: 107 947 107
Size: 840 3554
NgoMIV G'CCGG_C
Cuts at: 344 2573 344
Size: 2229 2165
NheI G'CTAG_C
Cuts at: 1266 1266
Size: 4394
NspI r_CATG'y
Cuts at: 960 1099 1447 1947 2854 960
Size: 139 348 500 907 2500
Fragments arranged by size:
2500 907 500 348 139
PciI A'CATG_T
Cuts at: 956 2850 956
Size: 1894 2500
PfoI T'CCnGG_A
Cuts at: 961 1172 961
Size: 211 4183
PmeI GTTT'AAAC
Cuts at: 1393 1393
Size: 4394
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3533 3565 3533
Size: 32 4362
PstI C_TGCA'G
Cuts at: 1199 1441 2126 1199
Size: 242 685 3467
Fragments arranged by size:
3467 685 242
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4394
PvuII CAG'CTG
Cuts at: 1819 2179 1819
Size: 360 4034
RsrII CG'GwC_CG
Cuts at: 2589 2589
Size: 4394
SacI G_AGCT'C
Cuts at: 1408 1408
Size: 4394
SalI G'TCGA_C
Cuts at: 1431 1431
Size: 4394
SapI GCTCTTCn'nnn_
Cuts at: 2417 2627 2417
Size: 210 4184
SbfI CC_TGCA'GG
Cuts at: 1441 1441
Size: 4394
ScaI AGT'ACT
Cuts at: 3911 3911
Size: 4394
SexAI A'CCwGG_T
Cuts at: 803 803
Size: 4394
SfcI C'TryA_G
Cuts at: 33 1195 1437 2122 3115 3306 33
Size: 1162 242 685 993 191 1121
Fragments arranged by size:
1162 1121 993 685 242 191
SfoI GGC'GCC
Cuts at: 1826 2074 1826
Size: 248 4146
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4394
SmaI CCC'GGG
Cuts at: 1229 1416 1229
Size: 187 4207
SmlI C'TyrA_G
Cuts at: 1549 2464 2956 3218 1549
Size: 915 492 262 2725
Fragments arranged by size:
2725 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4394
SphI G_CATG'C
Cuts at: 1447 1947 1447
Size: 500 3894
StuI AGG'CCT
Cuts at: 340 451 340
Size: 111 4283
StyI C'CwwG_G
Cuts at: 107 314 947 1528 1882 107
Size: 207 633 581 354 2619
Fragments arranged by size:
2619 633 581 354 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 209 1977 2110 2569 2778 209
Size: 1768 133 459 209 1825
Fragments arranged by size:
1825 1768 459 209 133
TatI w'GTAC_w
Cuts at: 3909 3909
Size: 4394
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3911 3943 3911
Size: 32 4362
Tth111I GACn'n_nGTC
Cuts at: 805 2191 805
Size: 1386 3008
XbaI T'CTAG_A
Cuts at: 1425 1425
Size: 4394
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 829 1889 114
Size: 715 1060 2619
Fragments arranged by size:
2619 1060 715
XmaI C'CCGG_G
Cuts at: 1227 1414 1227
Size: 187 4207
XmnI GAAnn'nnTTC
Cuts at: 382 382
Size: 4394
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PciI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AleI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BfrBI Bpu10I BsiWI BsmI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NotI NruI
NsiI PacI PasI PflMI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI