(Circular) (Six-base) MAPSORT of: pF1KB7714.seq Check: 7038 from: 1 to: 4532 pF1KB7714 4532 bp With 182 enzymes: SgfI * October 7, 2009 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 165 1150 165 Size: 985 3547 AccI GT'mk_AC Cuts at: 1570 1570 Size: 4532 Acc65I G'GTAC_C Cuts at: 1548 1548 Size: 4532 AclI AA'CG_TT Cuts at: 4372 4372 Size: 4532 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 999 1072 1234 1363 1417 2357 2789 3536 3804 Size: 73 162 129 54 940 432 747 268 Cuts at: 3804 999 Size: 1727 Fragments arranged by size: 1727 940 747 432 268 162 129 73 54 AflIII A'CryG_T Cuts at: 394 959 971 1884 2988 3965 394 Size: 565 12 913 1104 977 961 Fragments arranged by size: 1104 977 961 913 565 12 AgeI A'CCGG_T Cuts at: 2920 2920 Size: 4532 AhdI GACnn_n'nnGTC Cuts at: 285 285 Size: 4532 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 194 194 Size: 4532 AlwNI CAG_nnn'CTG Cuts at: 289 636 651 767 890 1455 1837 3404 289 Size: 347 15 116 123 565 382 1567 1417 Fragments arranged by size: 1567 1417 565 382 347 123 116 15 ApaLI G'TGCA_C Cuts at: 3302 3302 Size: 4532 ApoI r'AATT_y Cuts at: 735 1198 1536 3885 4141 735 Size: 463 338 2349 256 1126 Fragments arranged by size: 2349 1126 463 338 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4532 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4532 AvaI C'yCGr_G Cuts at: 1552 1552 Size: 4532 BamHI G'GATC_C Cuts at: 1557 1557 Size: 4532 BanI G'GyrC_C Cuts at: 452 469 1548 1962 2210 2245 452 Size: 17 1079 414 248 35 2739 Fragments arranged by size: 2739 1079 414 248 35 17 BanII G_rGCy'C Cuts at: 1190 1424 1546 2576 1190 Size: 234 122 1030 3146 Fragments arranged by size: 3146 1030 234 122 BbeI G_GCGC'C Cuts at: 473 1966 2214 473 Size: 1493 248 2791 Fragments arranged by size: 2791 1493 248 BbsI GAAGACnn'nnnn_ Cuts at: 496 1157 496 Size: 661 3871 BbvCI CC'TCA_GC Cuts at: 448 448 Size: 4532 BciVI GTATCCnnnnn_n' Cuts at: 2430 3191 4532 2430 Size: 761 1341 2430 Fragments arranged by size: 2430 1341 761 BclI T'GATC_A Cuts at: 557 596 674 2052 557 Size: 39 78 1378 3037 Fragments arranged by size: 3037 1378 78 39 BglII A'GATC_T Cuts at: 837 929 1040 2047 4084 837 Size: 92 111 1007 2037 1285 Fragments arranged by size: 2037 1285 1007 111 92 BlpI GC'TnA_GC Cuts at: 757 1439 1644 757 Size: 682 205 3645 Fragments arranged by size: 3645 682 205 Bme1580I G_kGCm'C Cuts at: 457 2157 2250 3306 457 Size: 1700 93 1056 1683 Fragments arranged by size: 1700 1683 1056 93 BmrI ACTGGGnnnn_n' Cuts at: 299 646 1744 1825 2160 4304 299 Size: 347 1098 81 335 2144 527 Fragments arranged by size: 2144 1098 527 347 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 951 2899 951 Size: 1948 2584 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 247 553 1204 247 Size: 306 651 3575 Fragments arranged by size: 3575 651 306 Bpu10I CC'TnA_GC Cuts at: 448 448 Size: 4532 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 1708 2587 3079 3377 1708 Size: 879 492 298 2863 Fragments arranged by size: 2863 879 492 298 BsaI GGTCTCn'nnnn_ Cuts at: 228 861 4236 228 Size: 633 3375 524 Fragments arranged by size: 3375 633 524 BsaAI yAC'GTr Cuts at: 1885 1885 Size: 4532 BsaBI GATnn'nnATC Cuts at: 6 673 2046 4083 6 Size: 667 1373 2037 455 Fragments arranged by size: 2037 1373 667 455 BsaHI Gr'CG_yC Cuts at: 470 1963 2211 2913 4409 470 Size: 1493 248 702 1496 593 Fragments arranged by size: 1496 1493 702 593 248 BsaWI w'CCGG_w Cuts at: 461 830 1721 2242 2920 3194 3341 3752 461 Size: 369 891 521 678 274 147 411 1241 Fragments arranged by size: 1241 891 678 521 411 369 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1027 1057 1027 Size: 30 4502 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 595 673 1249 1252 595 Size: 78 576 3 3875 Fragments arranged by size: 3875 576 78 3 BseYI C'CCAG_C Cuts at: 906 1370 1448 1957 2715 3292 3788 906 Size: 464 78 509 758 577 496 1650 Fragments arranged by size: 1650 758 577 509 496 464 78 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 154 664 154 Size: 510 4022 BsiEI CG_ry'CG Cuts at: 104 2074 2120 3328 104 Size: 1970 46 1208 1308 Fragments arranged by size: 1970 1308 1208 46 BsiHKAI G_wGCw'C Cuts at: 156 847 1424 1546 2324 2514 3306 156 Size: 691 577 122 778 190 792 1382 Fragments arranged by size: 1382 792 778 691 577 190 122 BsmI GAATG_Cn' Cuts at: 1363 1363 Size: 4532 Bsp1286I G_dGCh'C Cuts at: 156 457 847 1190 1424 1546 2157 2250 2324 Size: 301 390 343 234 122 611 93 74 Cuts at: 2324 2514 2576 3306 156 Size: 190 62 730 1382 Fragments arranged by size: 1382 730 611 390 343 301 234 190 122 93 74 62 BspEI T'CCGG_A Cuts at: 461 830 3752 461 Size: 369 2922 1241 Fragments arranged by size: 2922 1241 369 BspHI T'CATG_A Cuts at: 389 3708 389 Size: 3319 1213 BspMI ACCTGCnnnn'nnnn_ Cuts at: 165 1150 1582 1796 2098 2479 165 Size: 985 432 214 302 381 2218 Fragments arranged by size: 2218 985 432 381 302 214 BsrBI CCG'CTC Cuts at: 50 539 2824 2878 4362 4527 50 Size: 489 2285 54 1484 165 55 Fragments arranged by size: 2285 1484 489 165 55 54 BsrDI GCAATG_nn' Cuts at: 2444 2444 Size: 4532 BsrFI r'CCGG_y Cuts at: 1130 2530 2711 2920 1130 Size: 1400 181 209 2742 Fragments arranged by size: 2742 1400 209 181 BsrGI T'GTAC_A Cuts at: 267 267 Size: 4532 BssSI C'ACGA_G Cuts at: 2803 3161 2803 Size: 358 4174 BstAPI GCAn_nnn'nTGC Cuts at: 767 1909 767 Size: 1142 3390 BstBI TT'CG_AA Cuts at: 2893 2893 Size: 4532 BstYI r'GATC_y Cuts at: 1 837 929 1040 1557 2024 2047 2382 2628 Size: 836 92 111 517 467 23 335 246 Cuts at: 2628 3640 3726 3738 4084 1 Size: 1012 86 12 346 449 Fragments arranged by size: 1012 836 517 467 449 346 335 246 111 92 86 23 12 BtgI C'CryG_G Cuts at: 575 575 Size: 4532 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2482 2663 2826 2482 Size: 181 163 4188 Fragments arranged by size: 4188 181 163 BtsI GCAGTG_nn' Cuts at: 768 891 1154 1775 768 Size: 123 263 621 3525 Fragments arranged by size: 3525 621 263 123 DraI TTT'AAA Cuts at: 1531 1531 Size: 4532 DraIII CAC_nnn'GTG Cuts at: 1305 1305 Size: 4532 DrdI GACnn_nn'nnGTC Cuts at: 2238 3096 2238 Size: 858 3674 EaeI y'GGCC_r Cuts at: 1128 2117 2291 2682 2709 4054 1128 Size: 989 174 391 27 1345 1606 Fragments arranged by size: 1606 1345 989 391 174 27 EagI C'GGCC_G Cuts at: 2117 2117 Size: 4532 EarI CTCTTCn'nnn_ Cuts at: 1273 2555 2765 1273 Size: 1282 210 3040 Fragments arranged by size: 3040 1282 210 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 2740 3050 3196 4342 2740 Size: 310 146 1146 2930 Fragments arranged by size: 2930 1146 310 146 EcoICRI GAG'CTC Cuts at: 1422 1544 1422 Size: 122 4410 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 247 553 999 1072 1204 1234 1363 1417 2357 Size: 306 446 73 132 30 129 54 940 Cuts at: 2357 2789 3536 3804 247 Size: 432 747 268 975 Fragments arranged by size: 975 940 747 446 432 306 268 132 129 73 54 30 EcoNI CCTnn'n_nnAGG Cuts at: 1014 1051 1498 1014 Size: 37 447 4048 Fragments arranged by size: 4048 447 37 EcoO109I rG'GnC_Cy Cuts at: 143 334 792 1671 143 Size: 191 458 879 3004 Fragments arranged by size: 3004 879 458 191 EcoRI G'AATT_C Cuts at: 1536 1536 Size: 4532 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2551 2551 Size: 4532 FspI TGC'GCA Cuts at: 523 1462 2313 523 Size: 939 851 2742 Fragments arranged by size: 2742 939 851 HaeII r_GCGC'y Cuts at: 473 1966 2214 3236 4215 473 Size: 1493 248 1022 979 790 Fragments arranged by size: 1493 1022 979 790 248 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 271 303 612 644 678 710 829 861 1027 Size: 32 309 32 34 32 119 32 166 Cuts at: 1027 1059 2374 2404 2406 2436 271 Size: 32 1315 30 2 30 2367 Fragments arranged by size: 2367 1315 309 166 119 34 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 312 965 1078 1571 312 Size: 653 113 493 3273 Fragments arranged by size: 3273 653 493 113 KasI G'GCGC_C Cuts at: 469 1962 2210 469 Size: 1493 248 2791 Fragments arranged by size: 2791 1493 248 KpnI G_GTAC'C Cuts at: 1552 1552 Size: 4532 MluI A'CGCG_T Cuts at: 3965 3965 Size: 4532 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 309 590 3203 3387 309 Size: 281 2613 184 1454 Fragments arranged by size: 2613 1454 281 184 MscI TGG'CCA Cuts at: 2293 2293 Size: 4532 MslI CAynn'nnrTG Cuts at: 383 1408 2521 2648 383 Size: 1025 1113 127 2267 Fragments arranged by size: 2267 1113 1025 127 MspA1I CmG'CkG Cuts at: 666 867 1227 1317 1452 1643 1957 2317 3330 Size: 201 360 90 135 191 314 360 1013 Cuts at: 3330 3575 666 Size: 245 1623 Fragments arranged by size: 1623 1013 360 360 314 245 201 191 135 90 NaeI GCC'GGC Cuts at: 1132 2713 1132 Size: 1581 2951 NarI GG'CG_CC Cuts at: 470 1963 2211 470 Size: 1493 248 2791 Fragments arranged by size: 2791 1493 248 NgoMIV G'CCGG_C Cuts at: 1130 2711 1130 Size: 1581 2951 NspI r_CATG'y Cuts at: 398 963 975 1212 1585 2085 2992 398 Size: 565 12 237 373 500 907 1938 Fragments arranged by size: 1938 907 565 500 373 237 12 PciI A'CATG_T Cuts at: 394 959 971 2988 394 Size: 565 12 2017 1938 Fragments arranged by size: 2017 1938 565 12 PfoI T'CCnGG_A Cuts at: 294 1466 294 Size: 1172 3360 PmeI GTTT'AAAC Cuts at: 1531 1531 Size: 4532 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 3671 Size: 32 4500 PpuMI rG'GwC_Cy Cuts at: 143 334 143 Size: 191 4341 PstI C_TGCA'G Cuts at: 791 866 1161 1226 1298 1322 1579 2264 791 Size: 75 295 65 72 24 257 685 3059 Fragments arranged by size: 3059 685 295 257 75 72 65 24 PvuI CG_AT'CG Cuts at: 104 104 Size: 4532 PvuII CAG'CTG Cuts at: 867 1227 1317 1957 2317 867 Size: 360 90 640 360 3082 Fragments arranged by size: 3082 640 360 360 90 RsrII CG'GwC_CG Cuts at: 2727 2727 Size: 4532 SacI G_AGCT'C Cuts at: 1424 1546 1424 Size: 122 4410 SalI G'TCGA_C Cuts at: 1569 1569 Size: 4532 SanDI GG'GwC_CC Cuts at: 143 143 Size: 4532 SapI GCTCTTCn'nnn_ Cuts at: 2555 2765 2555 Size: 210 4322 SbfI CC_TGCA'GG Cuts at: 1579 1579 Size: 4532 ScaI AGT'ACT Cuts at: 4049 4049 Size: 4532 SfcI C'TryA_G Cuts at: 33 787 862 1157 1222 1294 1318 1575 2260 Size: 754 75 295 65 72 24 257 685 Cuts at: 2260 3253 3444 33 Size: 993 191 1121 Fragments arranged by size: 1121 993 754 685 295 257 191 75 72 65 24 SfoI GGC'GCC Cuts at: 471 1964 2212 471 Size: 1493 248 2791 Fragments arranged by size: 2791 1493 248 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4532 SmaI CCC'GGG Cuts at: 1554 1554 Size: 4532 SmlI C'TyrA_G Cuts at: 1687 2602 3094 3356 1687 Size: 915 492 262 2863 Fragments arranged by size: 2863 915 492 262 SpeI A'CTAG_T Cuts at: 67 67 Size: 4532 SphI G_CATG'C Cuts at: 1585 2085 1585 Size: 500 4032 StuI AGG'CCT Cuts at: 511 511 Size: 4532 StyI C'CwwG_G Cuts at: 1666 2020 1666 Size: 354 4178 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 1462 2115 2248 2707 2916 1462 Size: 653 133 459 209 3078 Fragments arranged by size: 3078 653 459 209 133 TatI w'GTAC_w Cuts at: 267 4047 267 Size: 3780 752 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 597 629 4049 4081 597 Size: 32 3420 32 1048 Fragments arranged by size: 3420 1048 32 32 Tth111I GACn'n_nGTC Cuts at: 2329 2329 Size: 4532 XbaI T'CTAG_A Cuts at: 1563 1563 Size: 4532 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2027 2027 Size: 4532 XmaI C'CCGG_G Cuts at: 1552 1552 Size: 4532 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI PciI PfoI PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI Enzymes that do not cut: AatII AfeI AflII AleI AloI ApaI AscI AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI EcoRV FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI NotI NruI NsiI PacI PasI PflMI PmlI PshAI PsiI PspOMI PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI