Restriction map for pF1KB7714
 (Circular) (Six-base) MAPSORT of: pF1KB7714.seq  Check: 7038  from: 1  to: 4532



pF1KB7714  4532 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:24  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    165    1150     165 
   Size:        985    3547
 
AccI GT'mk_AC

Cuts at:   1570    1570 
   Size:       4532
 
Acc65I G'GTAC_C

Cuts at:   1548    1548 
   Size:       4532
 
AclI AA'CG_TT

Cuts at:   4372    4372 
   Size:       4532
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    999    1072    1234    1363    1417    2357    2789    3536    3804 
   Size:         73     162     129      54     940     432     747     268

Cuts at:   3804     999 
   Size:       1727

  Fragments arranged by size: 

                1727     940     747     432     268     162     129      73
                  54
 
AflIII A'CryG_T

Cuts at:    394     959     971    1884    2988    3965     394 
   Size:        565      12     913    1104     977     961

  Fragments arranged by size: 

                1104     977     961     913     565      12
 
AgeI A'CCGG_T

Cuts at:   2920    2920 
   Size:       4532
 
AhdI GACnn_n'nnGTC

Cuts at:    285     285 
   Size:       4532
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    194     194 
   Size:       4532
 
AlwNI CAG_nnn'CTG

Cuts at:    289     636     651     767     890    1455    1837    3404     289 
   Size:        347      15     116     123     565     382    1567    1417

  Fragments arranged by size: 

                1567    1417     565     382     347     123     116      15
            
 
ApaLI G'TGCA_C

Cuts at:   3302    3302 
   Size:       4532
 
ApoI r'AATT_y

Cuts at:    735    1198    1536    3885    4141     735 
   Size:        463     338    2349     256    1126

  Fragments arranged by size: 

                2349    1126     463     338     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4532
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4532
 
AvaI C'yCGr_G

Cuts at:   1552    1552 
   Size:       4532
 
BamHI G'GATC_C

Cuts at:   1557    1557 
   Size:       4532
 
BanI G'GyrC_C

Cuts at:    452     469    1548    1962    2210    2245     452 
   Size:         17    1079     414     248      35    2739

  Fragments arranged by size: 

                2739    1079     414     248      35      17
 
BanII G_rGCy'C

Cuts at:   1190    1424    1546    2576    1190 
   Size:        234     122    1030    3146

  Fragments arranged by size: 

                3146    1030     234     122
 
BbeI G_GCGC'C

Cuts at:    473    1966    2214     473 
   Size:       1493     248    2791

  Fragments arranged by size: 

                2791    1493     248
 
BbsI GAAGACnn'nnnn_

Cuts at:    496    1157     496 
   Size:        661    3871
 
BbvCI CC'TCA_GC

Cuts at:    448     448 
   Size:       4532
 
BciVI GTATCCnnnnn_n'

Cuts at:   2430    3191    4532    2430 
   Size:        761    1341    2430

  Fragments arranged by size: 

                2430    1341     761
 
BclI T'GATC_A

Cuts at:    557     596     674    2052     557 
   Size:         39      78    1378    3037

  Fragments arranged by size: 

                3037    1378      78      39
 
BglII A'GATC_T

Cuts at:    837     929    1040    2047    4084     837 
   Size:         92     111    1007    2037    1285

  Fragments arranged by size: 

                2037    1285    1007     111      92
 
BlpI GC'TnA_GC

Cuts at:    757    1439    1644     757 
   Size:        682     205    3645

  Fragments arranged by size: 

                3645     682     205
 
Bme1580I G_kGCm'C

Cuts at:    457    2157    2250    3306     457 
   Size:       1700      93    1056    1683

  Fragments arranged by size: 

                1700    1683    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    299     646    1744    1825    2160    4304     299 
   Size:        347    1098      81     335    2144     527

  Fragments arranged by size: 

                2144    1098     527     347     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    951    2899     951 
   Size:       1948    2584
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     553    1204     247 
   Size:        306     651    3575

  Fragments arranged by size: 

                3575     651     306
 
Bpu10I CC'TnA_GC

Cuts at:    448     448 
   Size:       4532
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1708    2587    3079    3377    1708 
   Size:        879     492     298    2863

  Fragments arranged by size: 

                2863     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    228     861    4236     228 
   Size:        633    3375     524

  Fragments arranged by size: 

                3375     633     524
 
BsaAI yAC'GTr

Cuts at:   1885    1885 
   Size:       4532
 
BsaBI GATnn'nnATC

Cuts at:      6     673    2046    4083       6 
   Size:        667    1373    2037     455

  Fragments arranged by size: 

                2037    1373     667     455
 
BsaHI Gr'CG_yC

Cuts at:    470    1963    2211    2913    4409     470 
   Size:       1493     248     702    1496     593

  Fragments arranged by size: 

                1496    1493     702     593     248
 
BsaWI w'CCGG_w

Cuts at:    461     830    1721    2242    2920    3194    3341    3752     461 
   Size:        369     891     521     678     274     147     411    1241

  Fragments arranged by size: 

                1241     891     678     521     411     369     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1027    1057    1027 
   Size:         30    4502
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    595     673    1249    1252     595 
   Size:         78     576       3    3875

  Fragments arranged by size: 

                3875     576      78       3
 
BseYI C'CCAG_C

Cuts at:    906    1370    1448    1957    2715    3292    3788     906 
   Size:        464      78     509     758     577     496    1650

  Fragments arranged by size: 

                1650     758     577     509     496     464      78
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    154     664     154 
   Size:        510    4022
 
BsiEI CG_ry'CG

Cuts at:    104    2074    2120    3328     104 
   Size:       1970      46    1208    1308

  Fragments arranged by size: 

                1970    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    156     847    1424    1546    2324    2514    3306     156 
   Size:        691     577     122     778     190     792    1382

  Fragments arranged by size: 

                1382     792     778     691     577     190     122
 
BsmI GAATG_Cn'

Cuts at:   1363    1363 
   Size:       4532
 
Bsp1286I G_dGCh'C

Cuts at:    156     457     847    1190    1424    1546    2157    2250    2324 
   Size:        301     390     343     234     122     611      93      74

Cuts at:   2324    2514    2576    3306     156 
   Size:        190      62     730    1382

  Fragments arranged by size: 

                1382     730     611     390     343     301     234     190
                 122      93      74      62
 
BspEI T'CCGG_A

Cuts at:    461     830    3752     461 
   Size:        369    2922    1241

  Fragments arranged by size: 

                2922    1241     369
 
BspHI T'CATG_A

Cuts at:    389    3708     389 
   Size:       3319    1213
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    165    1150    1582    1796    2098    2479     165 
   Size:        985     432     214     302     381    2218

  Fragments arranged by size: 

                2218     985     432     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     539    2824    2878    4362    4527      50 
   Size:        489    2285      54    1484     165      55

  Fragments arranged by size: 

                2285    1484     489     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2444    2444 
   Size:       4532
 
BsrFI r'CCGG_y

Cuts at:   1130    2530    2711    2920    1130 
   Size:       1400     181     209    2742

  Fragments arranged by size: 

                2742    1400     209     181
 
BsrGI T'GTAC_A

Cuts at:    267     267 
   Size:       4532
 
BssSI C'ACGA_G

Cuts at:   2803    3161    2803 
   Size:        358    4174
 
BstAPI GCAn_nnn'nTGC

Cuts at:    767    1909     767 
   Size:       1142    3390
 
BstBI TT'CG_AA

Cuts at:   2893    2893 
   Size:       4532
 
BstYI r'GATC_y

Cuts at:      1     837     929    1040    1557    2024    2047    2382    2628 
   Size:        836      92     111     517     467      23     335     246

Cuts at:   2628    3640    3726    3738    4084       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     836     517     467     449     346     335     246
                 111      92      86      23      12
 
BtgI C'CryG_G

Cuts at:    575     575 
   Size:       4532
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2482    2663    2826    2482 
   Size:        181     163    4188

  Fragments arranged by size: 

                4188     181     163
 
BtsI GCAGTG_nn'

Cuts at:    768     891    1154    1775     768 
   Size:        123     263     621    3525

  Fragments arranged by size: 

                3525     621     263     123
 
DraI TTT'AAA

Cuts at:   1531    1531 
   Size:       4532
 
DraIII CAC_nnn'GTG

Cuts at:   1305    1305 
   Size:       4532
 
DrdI GACnn_nn'nnGTC

Cuts at:   2238    3096    2238 
   Size:        858    3674
 
EaeI y'GGCC_r

Cuts at:   1128    2117    2291    2682    2709    4054    1128 
   Size:        989     174     391      27    1345    1606

  Fragments arranged by size: 

                1606    1345     989     391     174      27
 
EagI C'GGCC_G

Cuts at:   2117    2117 
   Size:       4532
 
EarI CTCTTCn'nnn_

Cuts at:   1273    2555    2765    1273 
   Size:       1282     210    3040

  Fragments arranged by size: 

                3040    1282     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2740    3050    3196    4342    2740 
   Size:        310     146    1146    2930

  Fragments arranged by size: 

                2930    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1422    1544    1422 
   Size:        122    4410
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    247     553     999    1072    1204    1234    1363    1417    2357 
   Size:        306     446      73     132      30     129      54     940

Cuts at:   2357    2789    3536    3804     247 
   Size:        432     747     268     975

  Fragments arranged by size: 

                 975     940     747     446     432     306     268     132
                 129      73      54      30
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1014    1051    1498    1014 
   Size:         37     447    4048

  Fragments arranged by size: 

                4048     447      37
 
EcoO109I rG'GnC_Cy

Cuts at:    143     334     792    1671     143 
   Size:        191     458     879    3004

  Fragments arranged by size: 

                3004     879     458     191
 
EcoRI G'AATT_C

Cuts at:   1536    1536 
   Size:       4532
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2551    2551 
   Size:       4532
 
FspI TGC'GCA

Cuts at:    523    1462    2313     523 
   Size:        939     851    2742

  Fragments arranged by size: 

                2742     939     851
 
HaeII r_GCGC'y

Cuts at:    473    1966    2214    3236    4215     473 
   Size:       1493     248    1022     979     790

  Fragments arranged by size: 

                1493    1022     979     790     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    271     303     612     644     678     710     829     861    1027 
   Size:         32     309      32      34      32     119      32     166

Cuts at:   1027    1059    2374    2404    2406    2436     271 
   Size:         32    1315      30       2      30    2367

  Fragments arranged by size: 

                2367    1315     309     166     119      34      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:    312     965    1078    1571     312 
   Size:        653     113     493    3273

  Fragments arranged by size: 

                3273     653     493     113
 
KasI G'GCGC_C

Cuts at:    469    1962    2210     469 
   Size:       1493     248    2791

  Fragments arranged by size: 

                2791    1493     248
 
KpnI G_GTAC'C

Cuts at:   1552    1552 
   Size:       4532
 
MluI A'CGCG_T

Cuts at:   3965    3965 
   Size:       4532
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    309     590    3203    3387     309 
   Size:        281    2613     184    1454

  Fragments arranged by size: 

                2613    1454     281     184
 
MscI TGG'CCA

Cuts at:   2293    2293 
   Size:       4532
 
MslI CAynn'nnrTG

Cuts at:    383    1408    2521    2648     383 
   Size:       1025    1113     127    2267

  Fragments arranged by size: 

                2267    1113    1025     127
 
MspA1I CmG'CkG

Cuts at:    666     867    1227    1317    1452    1643    1957    2317    3330 
   Size:        201     360      90     135     191     314     360    1013

Cuts at:   3330    3575     666 
   Size:        245    1623

  Fragments arranged by size: 

                1623    1013     360     360     314     245     201     191
                 135      90
 
NaeI GCC'GGC

Cuts at:   1132    2713    1132 
   Size:       1581    2951
 
NarI GG'CG_CC

Cuts at:    470    1963    2211     470 
   Size:       1493     248    2791

  Fragments arranged by size: 

                2791    1493     248
 
NgoMIV G'CCGG_C

Cuts at:   1130    2711    1130 
   Size:       1581    2951
 
NspI r_CATG'y

Cuts at:    398     963     975    1212    1585    2085    2992     398 
   Size:        565      12     237     373     500     907    1938

  Fragments arranged by size: 

                1938     907     565     500     373     237      12
 
PciI A'CATG_T

Cuts at:    394     959     971    2988     394 
   Size:        565      12    2017    1938

  Fragments arranged by size: 

                2017    1938     565      12
 
PfoI T'CCnGG_A

Cuts at:    294    1466     294 
   Size:       1172    3360
 
PmeI GTTT'AAAC

Cuts at:   1531    1531 
   Size:       4532
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3671    3703    3671 
   Size:         32    4500
 
PpuMI rG'GwC_Cy

Cuts at:    143     334     143 
   Size:        191    4341
 
PstI C_TGCA'G

Cuts at:    791     866    1161    1226    1298    1322    1579    2264     791 
   Size:         75     295      65      72      24     257     685    3059

  Fragments arranged by size: 

                3059     685     295     257      75      72      65      24
            
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4532
 
PvuII CAG'CTG

Cuts at:    867    1227    1317    1957    2317     867 
   Size:        360      90     640     360    3082

  Fragments arranged by size: 

                3082     640     360     360      90
 
RsrII CG'GwC_CG

Cuts at:   2727    2727 
   Size:       4532
 
SacI G_AGCT'C

Cuts at:   1424    1546    1424 
   Size:        122    4410
 
SalI G'TCGA_C

Cuts at:   1569    1569 
   Size:       4532
 
SanDI GG'GwC_CC

Cuts at:    143     143 
   Size:       4532
 
SapI GCTCTTCn'nnn_

Cuts at:   2555    2765    2555 
   Size:        210    4322
 
SbfI CC_TGCA'GG

Cuts at:   1579    1579 
   Size:       4532
 
ScaI AGT'ACT

Cuts at:   4049    4049 
   Size:       4532
 
SfcI C'TryA_G

Cuts at:     33     787     862    1157    1222    1294    1318    1575    2260 
   Size:        754      75     295      65      72      24     257     685

Cuts at:   2260    3253    3444      33 
   Size:        993     191    1121

  Fragments arranged by size: 

                1121     993     754     685     295     257     191      75
                  72      65      24
 
SfoI GGC'GCC

Cuts at:    471    1964    2212     471 
   Size:       1493     248    2791

  Fragments arranged by size: 

                2791    1493     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4532
 
SmaI CCC'GGG

Cuts at:   1554    1554 
   Size:       4532
 
SmlI C'TyrA_G

Cuts at:   1687    2602    3094    3356    1687 
   Size:        915     492     262    2863

  Fragments arranged by size: 

                2863     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4532
 
SphI G_CATG'C

Cuts at:   1585    2085    1585 
   Size:        500    4032
 
StuI AGG'CCT

Cuts at:    511     511 
   Size:       4532
 
StyI C'CwwG_G

Cuts at:   1666    2020    1666 
   Size:        354    4178
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1462    2115    2248    2707    2916    1462 
   Size:        653     133     459     209    3078

  Fragments arranged by size: 

                3078     653     459     209     133
 
TatI w'GTAC_w

Cuts at:    267    4047     267 
   Size:       3780     752
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    597     629    4049    4081     597 
   Size:         32    3420      32    1048

  Fragments arranged by size: 

                3420    1048      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2329    2329 
   Size:       4532
 
XbaI T'CTAG_A

Cuts at:   1563    1563 
   Size:       4532
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2027    2027 
   Size:       4532
 
XmaI C'CCGG_G

Cuts at:   1552    1552 
   Size:       4532
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI   NgoMIV     NspI     PciI     PfoI
     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI     SfcI     SfoI     SgfI
     SmaI     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AleI     AloI     ApaI     AscI    AvrII
     BaeI     BcgI    BfrBI     BglI    BmgBI     BmtI    BsiWI    BsmBI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI    PflMI     PmlI    PshAI
     PsiI   PspOMI    PspXI     PsrI    SacII    SexAI     SfiI    SgrAI
    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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