Restriction map for
pF1KB7710
(Circular) (Six-base) MAPSORT of: pF1KB7710.seq Check: 5001 from: 1 to: 4508
pF1KB7710 4508 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 827 1371 827
Size: 544 3964
AccI GT'mk_AC
Cuts at: 504 1546 504
Size: 1042 3466
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 2333 2765 3512 3780 148
Size: 2185 432 747 268 876
Fragments arranged by size:
2185 876 747 432 268
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AhdI GACnn_n'nnGTC
Cuts at: 1047 1047
Size: 4508
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 286 1339 286
Size: 1053 3455
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1464 1496 1464
Size: 32 4476
AlwNI CAG_nnn'CTG
Cuts at: 645 1813 3380 645
Size: 1168 1567 1773
Fragments arranged by size:
1773 1567 1168
ApaI G_GGCC'C
Cuts at: 252 337 252
Size: 85 4423
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 1512 3861 4117 1512
Size: 2349 256 1903
Fragments arranged by size:
2349 1903 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 258 1131 1311 1528 258
Size: 873 180 217 3238
Fragments arranged by size:
3238 873 217 180
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 1148 1315 1524 1938 2186 2221 1148
Size: 167 209 414 248 35 3435
Fragments arranged by size:
3435 414 248 209 167 35
BanII G_rGCy'C
Cuts at: 252 337 762 1415 1522 2552 252
Size: 85 425 653 107 1030 2208
Fragments arranged by size:
2208 1030 653 425 107 85
BbeI G_GCGC'C
Cuts at: 1152 1942 2190 1152
Size: 790 248 3470
Fragments arranged by size:
3470 790 248
BbsI GAAGACnn'nnnn_
Cuts at: 566 856 1046 1443 566
Size: 290 190 397 3631
Fragments arranged by size:
3631 397 290 190
BbvCI CC'TCA_GC
Cuts at: 679 679
Size: 4508
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 379 413 90
Size: 34 255 34 4185
Fragments arranged by size:
4185 255 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2406 3167 4508 2406
Size: 761 1341 2406
Fragments arranged by size:
2406 1341 761
BclI T'GATC_A
Cuts at: 1274 2028 1274
Size: 754 3754
BglII A'GATC_T
Cuts at: 374 2023 4060 374
Size: 1649 2037 822
Fragments arranged by size:
2037 1649 822
BlpI GC'TnA_GC
Cuts at: 1330 1620 1330
Size: 290 4218
Bme1580I G_kGCm'C
Cuts at: 252 329 337 1318 2133 2226 3282 252
Size: 77 8 981 815 93 1056 1478
Fragments arranged by size:
1478 1056 981 815 93 77 8
BmrI ACTGGGnnnn_n'
Cuts at: 1720 1801 2136 4280 1720
Size: 81 335 2144 1948
Fragments arranged by size:
2144 1948 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 196 667 709 796 196
Size: 471 42 87 3908
Fragments arranged by size:
3908 471 87 42
Bpu10I CC'TnA_GC
Cuts at: 679 679
Size: 4508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1684 2563 3055 3353 1684
Size: 879 492 298 2839
Fragments arranged by size:
2839 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 738 4212 738
Size: 3474 1034
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 1149 1262 1939 2187 2889 4385 1149
Size: 113 677 248 702 1496 1272
Fragments arranged by size:
1496 1272 702 677 248 113
BsaWI w'CCGG_w
Cuts at: 1697 2218 2896 3170 3317 3728 1697
Size: 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 749 769 779 799 911 941 749
Size: 20 10 20 112 30 4316
Fragments arranged by size:
4316 112 30 20 20 10
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 307 310 373 793 1131 1239 307
Size: 3 63 420 338 108 3576
Fragments arranged by size:
3576 420 338 108 63 3
BseYI C'CCAG_C
Cuts at: 339 1176 1295 1933 2691 3268 3764 339
Size: 837 119 638 758 577 496 1083
Fragments arranged by size:
1083 837 758 638 577 496 119
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1179 1179
Size: 4508
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 365 523 1522 2300 2490 3282 365
Size: 158 999 778 190 792 1591
Fragments arranged by size:
1591 999 792 778 190 158
BsmBI CGTCTCn'nnnn_
Cuts at: 499 499
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 252 329 337 365 523 762 1318 1415 1522
Size: 77 8 28 158 239 556 97 107
Cuts at: 1522 2133 2226 2300 2490 2552 3282 252
Size: 611 93 74 190 62 730 1478
Fragments arranged by size:
1478 730 611 556 239 190 158 107
97 93 77 74 62 28 8
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 3684 3684
Size: 4508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 827 1152 1178 1331 1371 1558 1772 2074 2455
Size: 325 26 153 40 187 214 302 381
Cuts at: 2455 827
Size: 2880
Fragments arranged by size:
2880 381 325 302 214 187 153 40
26
BsrBI CCG'CTC
Cuts at: 50 282 2800 2854 4338 4503 50
Size: 232 2518 54 1484 165 55
Fragments arranged by size:
2518 1484 232 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2420 2420
Size: 4508
BsrFI r'CCGG_y
Cuts at: 215 2506 2687 2896 215
Size: 2291 181 209 1827
Fragments arranged by size:
2291 1827 209 181
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 1885 1885
Size: 4508
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstEII G'GTnAC_C
Cuts at: 186 1383 186
Size: 1197 3311
BstYI r'GATC_y
Cuts at: 1 266 374 896 1337 1533 2000 2023 2358
Size: 265 108 522 441 196 467 23 335
Cuts at: 2358 2604 3616 3702 3714 4060 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 522 467 449 441 346 335 265
246 196 108 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 866 866
Size: 4508
BtgI C'CryG_G
Cuts at: 314 452 314
Size: 138 4370
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 672 2458 2639 2802 672
Size: 1786 181 163 2378
Fragments arranged by size:
2378 1786 181 163
BtsI GCAGTG_nn'
Cuts at: 1297 1427 1751 1297
Size: 130 324 4054
Fragments arranged by size:
4054 324 130
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DraIII CAC_nnn'GTG
Cuts at: 453 453
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 2214 3072 2214
Size: 858 3650
EaeI y'GGCC_r
Cuts at: 671 1208 2093 2267 2658 2685 4030 671
Size: 537 885 174 391 27 1345 1149
Fragments arranged by size:
1345 1149 885 537 391 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 1069 2531 2741 1069
Size: 1462 210 2836
Fragments arranged by size:
2836 1462 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1520 1520
Size: 4508
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 196 667 709 796 2333 2765 3512 3780
Size: 48 471 42 87 1537 432 747 268
Cuts at: 3780 148
Size: 876
Fragments arranged by size:
1537 876 747 471 432 268 87 48
42
EcoNI CCTnn'n_nnAGG
Cuts at: 466 466
Size: 4508
EcoO109I rG'GnC_Cy
Cuts at: 248 430 1647 248
Size: 182 1217 3109
Fragments arranged by size:
3109 1217 182
EcoRI G'AATT_C
Cuts at: 1512 1512
Size: 4508
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 396 2527 396
Size: 2131 2377
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 1152 1253 1942 2190 3212 4191 1152
Size: 101 689 248 1022 979 1469
Fragments arranged by size:
1469 1022 979 689 248 101
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 749 781 1033 1065 2350 2380 2382 2412 749
Size: 32 252 32 1285 30 2 30 2845
Fragments arranged by size:
2845 1285 252 32 32 30 30 2
HincII GTy'rAC
Cuts at: 505 828 1167 1547 505
Size: 323 339 380 3466
Fragments arranged by size:
3466 380 339 323
HindIII A'AGCT_T
Cuts at: 568 568
Size: 4508
KasI G'GCGC_C
Cuts at: 1148 1938 2186 1148
Size: 790 248 3470
Fragments arranged by size:
3470 790 248
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3179 3363 3179
Size: 184 4324
MscI TGG'CCA
Cuts at: 673 2269 673
Size: 1596 2912
MslI CAynn'nnrTG
Cuts at: 2497 2624 2497
Size: 127 4381
MspA1I CmG'CkG
Cuts at: 174 339 1157 1176 1329 1619 1933 2293 3306
Size: 165 818 19 153 290 314 360 1013
Cuts at: 3306 3551 174
Size: 245 1131
Fragments arranged by size:
1131 1013 818 360 314 290 245 165
153 19
NaeI GCC'GGC
Cuts at: 217 2689 217
Size: 2472 2036
NarI GG'CG_CC
Cuts at: 1149 1939 2187 1149
Size: 790 248 3470
Fragments arranged by size:
3470 790 248
NcoI C'CATG_G
Cuts at: 314 314
Size: 4508
NgoMIV G'CCGG_C
Cuts at: 215 2687 215
Size: 2472 2036
NspI r_CATG'y
Cuts at: 1561 2061 2968 1561
Size: 500 907 3101
Fragments arranged by size:
3101 907 500
PasI CC'CwG_GG
Cuts at: 330 330
Size: 4508
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PfoI T'CCnGG_A
Cuts at: 461 461
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PpuMI rG'GwC_Cy
Cuts at: 430 430
Size: 4508
PspOMI G'GGCC_C
Cuts at: 248 333 248
Size: 85 4423
PstI C_TGCA'G
Cuts at: 424 638 1175 1555 2240 424
Size: 214 537 380 685 2692
Fragments arranged by size:
2692 685 537 380 214
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 1176 1933 2293 1176
Size: 757 360 3391
Fragments arranged by size:
3391 757 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1522 1522
Size: 4508
SalI G'TCGA_C
Cuts at: 503 1545 503
Size: 1042 3466
SanDI GG'GwC_CC
Cuts at: 430 430
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 1069 2531 2741 1069
Size: 1462 210 2836
Fragments arranged by size:
2836 1462 210
SbfI CC_TGCA'GG
Cuts at: 1555 1555
Size: 4508
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 420 634 1171 1551 2236 3229 3420 33
Size: 387 214 537 380 685 993 191 1121
Fragments arranged by size:
1121 993 685 537 387 380 214 191
SfoI GGC'GCC
Cuts at: 1150 1940 2188 1150
Size: 790 248 3470
Fragments arranged by size:
3470 790 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1133 1313 1530 1133
Size: 180 217 4111
Fragments arranged by size:
4111 217 180
SmlI C'TyrA_G
Cuts at: 1663 2578 3070 3332 1663
Size: 915 492 262 2839
Fragments arranged by size:
2839 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4508
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StyI C'CwwG_G
Cuts at: 314 1642 1996 314
Size: 1328 354 2826
Fragments arranged by size:
2826 1328 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2091 2224 2683 2892 2091
Size: 133 459 209 3707
Fragments arranged by size:
3707 459 209 133
TatI w'GTAC_w
Cuts at: 4023 4023
Size: 4508
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 535 567 769 801 4025 4057 535
Size: 32 202 32 3224 32 986
Fragments arranged by size:
3224 986 202 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 886 1261 2305 886
Size: 375 1044 3089
Fragments arranged by size:
3089 1044 375
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1023 2003 1023
Size: 980 3528
XmaI C'CCGG_G
Cuts at: 1131 1311 1528 1131
Size: 180 217 4111
Fragments arranged by size:
4111 217 180
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI Bsu36I BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AleI AscI AvrII BaeI BfrBI
BglI BmgBI BmtI BsiWI BsmI BsrGI BssHII BstXI
BstZ17I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI XmnI ZraI