Restriction map for
pF1KB7707
(Circular) (Six-base) MAPSORT of: pF1KB7707.seq Check: 7996 from: 1 to: 4370
pF1KB7707 4370 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 490 490
Size: 4370
AccI GT'mk_AC
Cuts at: 1408 1408
Size: 4370
Acc65I G'GTAC_C
Cuts at: 1386 1386
Size: 4370
AclI AA'CG_TT
Cuts at: 4210 4210
Size: 4370
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 2195 2627 3374 3642 222
Size: 1973 432 747 268 950
Fragments arranged by size:
1973 950 747 432 268
AflIII A'CryG_T
Cuts at: 1722 2826 3803 1722
Size: 1104 977 2289
Fragments arranged by size:
2289 1104 977
AgeI A'CCGG_T
Cuts at: 2758 2758
Size: 4370
AhdI GACnn_n'nnGTC
Cuts at: 978 978
Size: 4370
AleI CACnn'nnGTG
Cuts at: 998 1089 998
Size: 91 4279
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 583 583
Size: 4370
AlwNI CAG_nnn'CTG
Cuts at: 816 912 1026 1675 3242 816
Size: 96 114 649 1567 1944
Fragments arranged by size:
1944 1567 649 114 96
ApaLI G'TGCA_C
Cuts at: 3140 3140
Size: 4370
ApoI r'AATT_y
Cuts at: 1374 3723 3979 1374
Size: 2349 256 1765
Fragments arranged by size:
2349 1765 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4370
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
AvaI C'yCGr_G
Cuts at: 1070 1390 1070
Size: 320 4050
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 371 404 371
Size: 33 4337
BamHI G'GATC_C
Cuts at: 1395 1395
Size: 4370
BanI G'GyrC_C
Cuts at: 1386 1800 2048 2083 1386
Size: 414 248 35 3673
Fragments arranged by size:
3673 414 248 35
BanII G_rGCy'C
Cuts at: 1323 1384 2414 1323
Size: 61 1030 3279
Fragments arranged by size:
3279 1030 61
BbeI G_GCGC'C
Cuts at: 1804 2052 1804
Size: 248 4122
BbsI GAAGACnn'nnnn_
Cuts at: 164 634 698 164
Size: 470 64 3836
Fragments arranged by size:
3836 470 64
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4336
BciVI GTATCCnnnnn_n'
Cuts at: 372 2268 3029 4370 372
Size: 1896 761 1341 372
Fragments arranged by size:
1896 1341 761 372
BclI T'GATC_A
Cuts at: 1890 1890
Size: 4370
BglII A'GATC_T
Cuts at: 1885 3922 1885
Size: 2037 2333
BlpI GC'TnA_GC
Cuts at: 938 1482 938
Size: 544 3826
Bme1580I G_kGCm'C
Cuts at: 1995 2088 3144 1995
Size: 93 1056 3221
Fragments arranged by size:
3221 1056 93
BmgBI CAC'GTC
Cuts at: 749 749
Size: 4370
BmrI ACTGGGnnnn_n'
Cuts at: 1582 1663 1998 4142 1582
Size: 81 335 2144 1810
Fragments arranged by size:
2144 1810 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2737 2737
Size: 4370
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 994 1265 994
Size: 271 4099
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1546 2425 2917 3215 1546
Size: 879 492 298 2701
Fragments arranged by size:
2701 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 328 332 1060 4074 328
Size: 4 728 3014 624
Fragments arranged by size:
3014 728 624 4
BsaAI yAC'GTr
Cuts at: 687 1723 687
Size: 1036 3334
BsaBI GATnn'nnATC
Cuts at: 6 1884 3921 6
Size: 1878 2037 455
Fragments arranged by size:
2037 1878 455
BsaHI Gr'CG_yC
Cuts at: 892 1801 2049 2751 4247 892
Size: 909 248 702 1496 1015
Fragments arranged by size:
1496 1015 909 702 248
BsaWI w'CCGG_w
Cuts at: 1559 2080 2758 3032 3179 3590 1559
Size: 521 678 274 147 411 2339
Fragments arranged by size:
2339 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 124 154 308 338 124
Size: 30 154 30 4156
Fragments arranged by size:
4156 154 30 30
BseYI C'CCAG_C
Cuts at: 1795 2553 3130 3626 1795
Size: 758 577 496 2539
Fragments arranged by size:
2539 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 507 507
Size: 4370
BsiEI CG_ry'CG
Cuts at: 104 1159 1912 1958 3166 104
Size: 1055 753 46 1208 1308
Fragments arranged by size:
1308 1208 1055 753 46
BsiHKAI G_wGCw'C
Cuts at: 193 1384 2162 2352 3144 193
Size: 1191 778 190 792 1419
Fragments arranged by size:
1419 1191 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 739 739
Size: 4370
Bsp1286I G_dGCh'C
Cuts at: 193 1323 1384 1995 2088 2162 2352 2414 3144
Size: 1130 61 611 93 74 190 62 730
Cuts at: 3144 193
Size: 1419
Fragments arranged by size:
1419 1130 730 611 190 93 74 62
61
BspEI T'CCGG_A
Cuts at: 3590 3590
Size: 4370
BspHI T'CATG_A
Cuts at: 3546 3546
Size: 4370
BspMI ACCTGCnnnn'nnnn_
Cuts at: 490 887 1420 1634 1936 2317 490
Size: 397 533 214 302 381 2543
Fragments arranged by size:
2543 533 397 381 302 214
BsrBI CCG'CTC
Cuts at: 50 430 1034 2662 2716 4200 4365 50
Size: 380 604 1628 54 1484 165 55
Fragments arranged by size:
1628 1484 604 380 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2282 2282
Size: 4370
BsrFI r'CCGG_y
Cuts at: 1158 2368 2549 2758 1158
Size: 1210 181 209 2770
Fragments arranged by size:
2770 1210 209 181
BssSI C'ACGA_G
Cuts at: 1344 2641 2999 1344
Size: 1297 358 2715
Fragments arranged by size:
2715 1297 358
BstAPI GCAn_nnn'nTGC
Cuts at: 774 816 912 1747 774
Size: 42 96 835 3397
Fragments arranged by size:
3397 835 96 42
BstBI TT'CG_AA
Cuts at: 2731 2731
Size: 4370
BstEII G'GTnAC_C
Cuts at: 210 756 1000 210
Size: 546 244 3580
Fragments arranged by size:
3580 546 244
BstXI CCAn_nnnn'nTGG
Cuts at: 150 687 1091 150
Size: 537 404 3429
Fragments arranged by size:
3429 537 404
BstYI r'GATC_y
Cuts at: 1 1211 1395 1862 1885 2220 2466 3478 3564
Size: 1210 184 467 23 335 246 1012 86
Cuts at: 3564 3576 3922 1
Size: 12 346 449
Fragments arranged by size:
1210 1012 467 449 346 335 246 184
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1134 1295 1134
Size: 161 4209
BtgI C'CryG_G
Cuts at: 143 383 143
Size: 240 4130
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2320 2501 2664 2320
Size: 181 163 4026
Fragments arranged by size:
4026 181 163
BtsI GCAGTG_nn'
Cuts at: 785 1613 785
Size: 828 3542
DraI TTT'AAA
Cuts at: 1369 1369
Size: 4370
DrdI GACnn_nn'nnGTC
Cuts at: 2076 2934 2076
Size: 858 3512
EaeI y'GGCC_r
Cuts at: 431 1156 1160 1955 2129 2520 2547 3892 431
Size: 725 4 795 174 391 27 1345 909
Fragments arranged by size:
1345 909 795 725 391 174 27 4
EagI C'GGCC_G
Cuts at: 1156 1955 1156
Size: 799 3571
EarI CTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2578 2888 3034 4180 2578
Size: 310 146 1146 2768
Fragments arranged by size:
2768 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1382 1382
Size: 4370
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 222 994 1265 2195 2627 3374 3642 222
Size: 772 271 930 432 747 268 950
Fragments arranged by size:
950 930 772 747 432 271 268
EcoO109I rG'GnC_Cy
Cuts at: 222 1509 222
Size: 1287 3083
EcoRI G'AATT_C
Cuts at: 1374 1374
Size: 4370
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2389 2389
Size: 4370
FseI GG_CCGG'CC
Cuts at: 1162 1162
Size: 4370
FspI TGC'GCA
Cuts at: 2151 2151
Size: 4370
HaeII r_GCGC'y
Cuts at: 1804 2052 3074 4053 1804
Size: 248 1022 979 2121
Fragments arranged by size:
2121 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 124 156 586 618 696 728 964 996 2212
Size: 32 430 32 78 32 236 32 1216
Cuts at: 2212 2242 2244 2274 124
Size: 30 2 30 2220
Fragments arranged by size:
2220 1216 430 236 78 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1409 1409
Size: 4370
HindIII A'AGCT_T
Cuts at: 659 659
Size: 4370
KasI G'GCGC_C
Cuts at: 1800 2048 1800
Size: 248 4122
KpnI G_GTAC'C
Cuts at: 1390 1390
Size: 4370
MluI A'CGCG_T
Cuts at: 3803 3803
Size: 4370
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 218 3041 3225 218
Size: 2823 184 1363
Fragments arranged by size:
2823 1363 184
MscI TGG'CCA
Cuts at: 2131 2131
Size: 4370
MslI CAynn'nnrTG
Cuts at: 565 606 998 1089 2359 2486 565
Size: 41 392 91 1270 127 2449
Fragments arranged by size:
2449 1270 392 127 91 41
MspA1I CmG'CkG
Cuts at: 125 183 385 945 1481 1795 2155 3168 3413
Size: 58 202 560 536 314 360 1013 245
Cuts at: 3413 125
Size: 1082
Fragments arranged by size:
1082 1013 560 536 360 314 245 202
58
NaeI GCC'GGC
Cuts at: 1160 2551 1160
Size: 1391 2979
NarI GG'CG_CC
Cuts at: 1801 2049 1801
Size: 248 4122
NgoMIV G'CCGG_C
Cuts at: 1158 2549 1158
Size: 1391 2979
NspI r_CATG'y
Cuts at: 570 1194 1423 1923 2830 570
Size: 624 229 500 907 2110
Fragments arranged by size:
2110 907 624 500 229
PasI CC'CwG_GG
Cuts at: 1146 1146
Size: 4370
PciI A'CATG_T
Cuts at: 2826 2826
Size: 4370
PflMI CCAn_nnn'nTGG
Cuts at: 1026 1026
Size: 4370
PmeI GTTT'AAAC
Cuts at: 1369 1369
Size: 4370
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3509 3541 3509
Size: 32 4338
PpuMI rG'GwC_Cy
Cuts at: 222 222
Size: 4370
PshAI GACnn'nnGTC
Cuts at: 344 344
Size: 4370
PstI C_TGCA'G
Cuts at: 359 821 828 908 1417 2102 359
Size: 462 7 80 509 685 2627
Fragments arranged by size:
2627 685 509 462 80 7
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4370
PvuII CAG'CTG
Cuts at: 183 945 1795 2155 183
Size: 762 850 360 2398
Fragments arranged by size:
2398 850 762 360
RsrII CG'GwC_CG
Cuts at: 2565 2565
Size: 4370
SacI G_AGCT'C
Cuts at: 1384 1384
Size: 4370
SacII CC_GC'GG
Cuts at: 386 386
Size: 4370
SalI G'TCGA_C
Cuts at: 1407 1407
Size: 4370
SapI GCTCTTCn'nnn_
Cuts at: 2393 2603 2393
Size: 210 4160
SbfI CC_TGCA'GG
Cuts at: 908 1417 908
Size: 509 3861
ScaI AGT'ACT
Cuts at: 3887 3887
Size: 4370
SfcI C'TryA_G
Cuts at: 33 355 817 824 904 1413 2098 3091 3282
Size: 322 462 7 80 509 685 993 191
Cuts at: 3282 33
Size: 1121
Fragments arranged by size:
1121 993 685 509 462 322 191 80
7
SfoI GGC'GCC
Cuts at: 1802 2050 1802
Size: 248 4122
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4370
SmaI CCC'GGG
Cuts at: 1392 1392
Size: 4370
SmlI C'TyrA_G
Cuts at: 1525 2440 2932 3194 1525
Size: 915 492 262 2701
Fragments arranged by size:
2701 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4370
SphI G_CATG'C
Cuts at: 1423 1923 1423
Size: 500 3870
StyI C'CwwG_G
Cuts at: 1504 1858 1504
Size: 354 4016
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 452 1953 2086 2545 2754 452
Size: 1501 133 459 209 2068
Fragments arranged by size:
2068 1501 459 209 133
TatI w'GTAC_w
Cuts at: 3885 3885
Size: 4370
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 581 613 3887 3919 581
Size: 32 3274 32 1032
Fragments arranged by size:
3274 1032 32 32
Tth111I GACn'n_nGTC
Cuts at: 2167 2167
Size: 4370
XbaI T'CTAG_A
Cuts at: 1401 1401
Size: 4370
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 150 1865 150
Size: 1715 2655
XmaI C'CCGG_G
Cuts at: 1390 1390
Size: 4370
XmnI GAAnn'nnTTC
Cuts at: 1128 1128
Size: 4370
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI
BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI
BclI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FseI FspI HaeII Hin4I HincII HindIII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PstI PvuI PvuII RsrII SacI SacII SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AloI ApaI AscI AvrII BbvCI
BfrBI BglI BmtI Bpu10I BseRI BsiWI BsmI BsrGI
BssHII BstZ17I ClaI CspCI DraIII EcoNI EcoRV FspAI
HpaI MfeI NcoI NdeI NheI NotI NruI NsiI
PacI PfoI PmlI PsiI PspOMI PspXI PsrI SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI