Restriction map for
pF1KB7706
(Circular) (Six-base) MAPSORT of: pF1KB7706.seq Check: 5882 from: 1 to: 4508
pF1KB7706 4508 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AatII G_ACGT'C
Cuts at: 115 115
Size: 4508
AccI GT'mk_AC
Cuts at: 932 1546 932
Size: 614 3894
Acc65I G'GTAC_C
Cuts at: 1524 1524
Size: 4508
AclI AA'CG_TT
Cuts at: 4348 4348
Size: 4508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1423 2333 2765 3512 3780 1423
Size: 910 432 747 268 2151
Fragments arranged by size:
2151 910 747 432 268
AflIII A'CryG_T
Cuts at: 1860 2964 3941 1860
Size: 1104 977 2427
Fragments arranged by size:
2427 1104 977
AgeI A'CCGG_T
Cuts at: 2896 2896
Size: 4508
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1091 1123 1091
Size: 32 4476
AlwNI CAG_nnn'CTG
Cuts at: 1238 1281 1446 1813 3380 1238
Size: 43 165 367 1567 2366
Fragments arranged by size:
2366 1567 367 165 43
ApaLI G'TGCA_C
Cuts at: 3278 3278
Size: 4508
ApoI r'AATT_y
Cuts at: 439 544 1250 1512 3861 4117 439
Size: 105 706 262 2349 256 830
Fragments arranged by size:
2349 830 706 262 256 105
AseI AT'TA_AT
Cuts at: 20 20
Size: 4508
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
AvaI C'yCGr_G
Cuts at: 1528 1528
Size: 4508
BamHI G'GATC_C
Cuts at: 1533 1533
Size: 4508
BanI G'GyrC_C
Cuts at: 247 1524 1938 2186 2221 247
Size: 1277 414 248 35 2534
Fragments arranged by size:
2534 1277 414 248 35
BanII G_rGCy'C
Cuts at: 1325 1474 1522 2552 1325
Size: 149 48 1030 3281
Fragments arranged by size:
3281 1030 149 48
BbeI G_GCGC'C
Cuts at: 1942 2190 1942
Size: 248 4260
BciVI GTATCCnnnnn_n'
Cuts at: 825 2406 3167 4508 825
Size: 1581 761 1341 825
Fragments arranged by size:
1581 1341 825 761
BclI T'GATC_A
Cuts at: 798 2028 798
Size: 1230 3278
BfrBI ATG'CAT
Cuts at: 973 973
Size: 4508
BglII A'GATC_T
Cuts at: 316 2023 4060 316
Size: 1707 2037 764
Fragments arranged by size:
2037 1707 764
BlpI GC'TnA_GC
Cuts at: 1274 1620 1274
Size: 346 4162
Bme1580I G_kGCm'C
Cuts at: 2133 2226 3282 2133
Size: 93 1056 3359
Fragments arranged by size:
3359 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 860 1720 1801 2136 4280 860
Size: 860 81 335 2144 1088
Fragments arranged by size:
2144 1088 860 335 81
BmtI G_CTAG'C
Cuts at: 704 704
Size: 4508
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2875 2875
Size: 4508
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 503 283
Size: 220 4288
Bpu10I CC'TnA_GC
Cuts at: 586 586
Size: 4508
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 294 1684 2563 3055 3353 294
Size: 1390 879 492 298 1449
Fragments arranged by size:
1449 1390 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 4212 4212
Size: 4508
BsaAI yAC'GTr
Cuts at: 1861 1861
Size: 4508
BsaBI GATnn'nnATC
Cuts at: 6 2022 4059 6
Size: 2016 2037 455
Fragments arranged by size:
2037 2016 455
BsaHI Gr'CG_yC
Cuts at: 112 330 1939 2187 2889 4385 112
Size: 218 1609 248 702 1496 235
Fragments arranged by size:
1609 1496 702 248 235 218
BsaWI w'CCGG_w
Cuts at: 1697 2218 2896 3170 3317 3728 1697
Size: 521 678 274 147 411 2477
Fragments arranged by size:
2477 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 759 789 759
Size: 30 4478
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 199 202 764 199
Size: 3 562 3943
Fragments arranged by size:
3943 562 3
BseYI C'CCAG_C
Cuts at: 723 759 821 1231 1933 2691 3268 3764 723
Size: 36 62 410 702 758 577 496 1467
Fragments arranged by size:
1467 758 702 577 496 410 62 36
BsiEI CG_ry'CG
Cuts at: 104 2050 2096 3304 104
Size: 1946 46 1208 1308
Fragments arranged by size:
1946 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1325 1522 2300 2490 3282 1325
Size: 197 778 190 792 2551
Fragments arranged by size:
2551 792 778 197 190
BsmI GAATG_Cn'
Cuts at: 1150 1150
Size: 4508
Bsp1286I G_dGCh'C
Cuts at: 1325 1474 1522 2133 2226 2300 2490 2552 3282
Size: 149 48 611 93 74 190 62 730
Cuts at: 3282 1325
Size: 2551
Fragments arranged by size:
2551 730 611 190 149 93 74 62
48
BspEI T'CCGG_A
Cuts at: 3728 3728
Size: 4508
BspHI T'CATG_A
Cuts at: 411 3684 411
Size: 3273 1235
BspMI ACCTGCnnnn'nnnn_
Cuts at: 327 1558 1772 2074 2455 327
Size: 1231 214 302 381 2380
Fragments arranged by size:
2380 1231 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2800 2854 4338 4503 50
Size: 2750 54 1484 165 55
Fragments arranged by size:
2750 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2420 2420
Size: 4508
BsrFI r'CCGG_y
Cuts at: 1382 2506 2687 2896 1382
Size: 1124 181 209 2994
Fragments arranged by size:
2994 1124 209 181
BsrGI T'GTAC_A
Cuts at: 1170 1170
Size: 4508
BssSI C'ACGA_G
Cuts at: 2779 3137 2779
Size: 358 4150
BstAPI GCAn_nnn'nTGC
Cuts at: 214 1885 214
Size: 1671 2837
BstBI TT'CG_AA
Cuts at: 2869 2869
Size: 4508
BstYI r'GATC_y
Cuts at: 1 316 451 1008 1533 2000 2023 2358 2604
Size: 315 135 557 525 467 23 335 246
Cuts at: 2604 3616 3702 3714 4060 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 557 525 467 449 346 335 315
246 135 86 23 12
BtgI C'CryG_G
Cuts at: 251 1299 251
Size: 1048 3460
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2458 2639 2802 2458
Size: 181 163 4164
Fragments arranged by size:
4164 181 163
BtsI GCAGTG_nn'
Cuts at: 1751 1751
Size: 4508
DraI TTT'AAA
Cuts at: 1507 1507
Size: 4508
DrdI GACnn_nn'nnGTC
Cuts at: 743 2214 3072 743
Size: 1471 858 2179
Fragments arranged by size:
2179 1471 858
EaeI y'GGCC_r
Cuts at: 119 391 2093 2267 2658 2685 4030 119
Size: 272 1702 174 391 27 1345 597
Fragments arranged by size:
1702 1345 597 391 272 174 27
EagI C'GGCC_G
Cuts at: 2093 2093
Size: 4508
EarI CTCTTCn'nnn_
Cuts at: 899 1442 2531 2741 899
Size: 543 1089 210 2666
Fragments arranged by size:
2666 1089 543 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2716 3026 3172 4318 2716
Size: 310 146 1146 2906
Fragments arranged by size:
2906 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1323 1520 1323
Size: 197 4311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 283 503 1423 2333 2765 3512 3780 283
Size: 220 920 910 432 747 268 1011
Fragments arranged by size:
1011 920 910 747 432 268 220
EcoO109I rG'GnC_Cy
Cuts at: 1647 1647
Size: 4508
EcoRI G'AATT_C
Cuts at: 544 1512 544
Size: 968 3540
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2527 2527
Size: 4508
FspI TGC'GCA
Cuts at: 2289 2289
Size: 4508
HaeII r_GCGC'y
Cuts at: 390 1942 2190 3212 4191 390
Size: 1552 248 1022 979 707
Fragments arranged by size:
1552 1022 979 707 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2350 2380 2382 2412 2350
Size: 30 2 30 4446
Fragments arranged by size:
4446 30 30 2
HincII GTy'rAC
Cuts at: 1296 1547 1296
Size: 251 4257
HindIII A'AGCT_T
Cuts at: 962 962
Size: 4508
KasI G'GCGC_C
Cuts at: 1938 2186 1938
Size: 248 4260
KpnI G_GTAC'C
Cuts at: 1528 1528
Size: 4508
MfeI C'AATT_G
Cuts at: 1073 1073
Size: 4508
MluI A'CGCG_T
Cuts at: 3941 3941
Size: 4508
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 407 936 1390 3179 3363 407
Size: 529 454 1789 184 1552
Fragments arranged by size:
1789 1552 529 454 184
MscI TGG'CCA
Cuts at: 121 2269 121
Size: 2148 2360
MslI CAynn'nnrTG
Cuts at: 1330 1345 1427 2497 2624 1330
Size: 15 82 1070 127 3214
Fragments arranged by size:
3214 1070 127 82 15
MspA1I CmG'CkG
Cuts at: 763 1238 1619 1933 2293 3306 3551 763
Size: 475 381 314 360 1013 245 1720
Fragments arranged by size:
1720 1013 475 381 360 314 245
NaeI GCC'GGC
Cuts at: 2689 2689
Size: 4508
NarI GG'CG_CC
Cuts at: 1939 2187 1939
Size: 248 4260
NcoI C'CATG_G
Cuts at: 251 1299 251
Size: 1048 3460
NgoMIV G'CCGG_C
Cuts at: 2687 2687
Size: 4508
NheI G'CTAG_C
Cuts at: 700 700
Size: 4508
NsiI A_TGCA'T
Cuts at: 975 975
Size: 4508
NspI r_CATG'y
Cuts at: 609 1561 2061 2968 609
Size: 952 500 907 2149
Fragments arranged by size:
2149 952 907 500
PciI A'CATG_T
Cuts at: 2964 2964
Size: 4508
PflMI CCAn_nnn'nTGG
Cuts at: 828 828
Size: 4508
PfoI T'CCnGG_A
Cuts at: 282 282
Size: 4508
PmeI GTTT'AAAC
Cuts at: 1507 1507
Size: 4508
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3647 3679 3647
Size: 32 4476
PstI C_TGCA'G
Cuts at: 338 1243 1555 2240 338
Size: 905 312 685 2606
Fragments arranged by size:
2606 905 685 312
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4508
PvuII CAG'CTG
Cuts at: 763 1238 1933 2293 763
Size: 475 695 360 2978
Fragments arranged by size:
2978 695 475 360
RsrII CG'GwC_CG
Cuts at: 2703 2703
Size: 4508
SacI G_AGCT'C
Cuts at: 1325 1522 1325
Size: 197 4311
SalI G'TCGA_C
Cuts at: 1545 1545
Size: 4508
SapI GCTCTTCn'nnn_
Cuts at: 899 2531 2741 899
Size: 1632 210 2666
Fragments arranged by size:
2666 1632 210
SbfI CC_TGCA'GG
Cuts at: 338 1555 338
Size: 1217 3291
ScaI AGT'ACT
Cuts at: 4025 4025
Size: 4508
SfcI C'TryA_G
Cuts at: 33 334 1239 1551 2236 3229 3420 33
Size: 301 905 312 685 993 191 1121
Fragments arranged by size:
1121 993 905 685 312 301 191
SfoI GGC'GCC
Cuts at: 1940 2188 1940
Size: 248 4260
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4508
SmaI CCC'GGG
Cuts at: 1530 1530
Size: 4508
SmlI C'TyrA_G
Cuts at: 273 1663 2578 3070 3332 273
Size: 1390 915 492 262 1449
Fragments arranged by size:
1449 1390 915 492 262
SpeI A'CTAG_T
Cuts at: 67 139 982 67
Size: 72 843 3593
Fragments arranged by size:
3593 843 72
SphI G_CATG'C
Cuts at: 1561 2061 1561
Size: 500 4008
StyI C'CwwG_G
Cuts at: 251 1299 1642 1996 251
Size: 1048 343 354 2763
Fragments arranged by size:
2763 1048 354 343
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 2091 2224 2683 2892 2091
Size: 133 459 209 3707
Fragments arranged by size:
3707 459 209 133
TatI w'GTAC_w
Cuts at: 1115 1170 4023 1115
Size: 55 2853 1600
Fragments arranged by size:
2853 1600 55
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 162 194 645 677 4025 4057 162
Size: 32 451 32 3348 32 613
Fragments arranged by size:
3348 613 451 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 2305 2305
Size: 4508
XbaI T'CTAG_A
Cuts at: 1539 1539
Size: 4508
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 258 2003 114
Size: 144 1745 2619
Fragments arranged by size:
2619 1745 144
XmaI C'CCGG_G
Cuts at: 1528 1528
Size: 4508
XmnI GAAnn'nnTTC
Cuts at: 965 965
Size: 4508
ZraI GAC'GTC
Cuts at: 113 113
Size: 4508
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflIII AgeI AloI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BfrBI BglII BlpI Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NheI NsiI NspI PciI
PflMI PfoI PmeI PpiI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AflII AhdI AleI AlfI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BglI BmgBI BsgI
BsiWI BsmBI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoNI EcoRV FseI FspAI HpaI NdeI
NotI NruI PacI PasI PmlI PpuMI PshAI PsiI
PspOMI PspXI PsrI SacII SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI