Restriction map for
pF1KB7703
(Circular) (Six-base) MAPSORT of: pF1KB7703.seq Check: 4568 from: 1 to: 4361
pF1KB7703 4361 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1139 1139
Size: 4361
AatII G_ACGT'C
Cuts at: 506 506
Size: 4361
AccI GT'mk_AC
Cuts at: 1399 1399
Size: 4361
Acc65I G'GTAC_C
Cuts at: 906 1377 906
Size: 471 3890
AclI AA'CG_TT
Cuts at: 1355 4201 1355
Size: 2846 1515
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 248 2186 2618 3365 3633 248
Size: 1938 432 747 268 976
Fragments arranged by size:
1938 976 747 432 268
AfeI AGC'GCT
Cuts at: 679 679
Size: 4361
AflIII A'CryG_T
Cuts at: 1713 2817 3794 1713
Size: 1104 977 2280
Fragments arranged by size:
2280 1104 977
AgeI A'CCGG_T
Cuts at: 2749 2749
Size: 4361
AhdI GACnn_n'nnGTC
Cuts at: 1209 1209
Size: 4361
AleI CACnn'nnGTG
Cuts at: 1145 1235 1145
Size: 90 4271
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 916 948 916
Size: 32 4329
AlwNI CAG_nnn'CTG
Cuts at: 254 814 861 1666 3233 254
Size: 560 47 805 1567 1382
Fragments arranged by size:
1567 1382 805 560 47
ApaI G_GGCC'C
Cuts at: 428 727 739 428
Size: 299 12 4050
Fragments arranged by size:
4050 299 12
ApaLI G'TGCA_C
Cuts at: 1312 3131 1312
Size: 1819 2542
ApoI r'AATT_y
Cuts at: 1365 3714 3970 1365
Size: 2349 256 1756
Fragments arranged by size:
2349 1756 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4361
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
AvaI C'yCGr_G
Cuts at: 475 1381 475
Size: 906 3455
BamHI G'GATC_C
Cuts at: 1386 1386
Size: 4361
BanI G'GyrC_C
Cuts at: 313 700 715 906 1377 1791 2039 2074 313
Size: 387 15 191 471 414 248 35 2600
Fragments arranged by size:
2600 471 414 387 248 191 35 15
BanII G_rGCy'C
Cuts at: 428 727 739 1375 2405 428
Size: 299 12 636 1030 2384
Fragments arranged by size:
2384 1030 636 299 12
BbeI G_GCGC'C
Cuts at: 1795 2043 1795
Size: 248 4113
BciVI GTATCCnnnnn_n'
Cuts at: 2259 3020 4361 2259
Size: 761 1341 2259
Fragments arranged by size:
2259 1341 761
BclI T'GATC_A
Cuts at: 1881 1881
Size: 4361
BglII A'GATC_T
Cuts at: 1876 3913 1876
Size: 2037 2324
BlpI GC'TnA_GC
Cuts at: 1473 1473
Size: 4361
Bme1580I G_kGCm'C
Cuts at: 316 428 720 727 739 808 899 962 1316
Size: 112 292 7 12 69 91 63 354
Cuts at: 1316 1986 2079 3135 316
Size: 670 93 1056 1542
Fragments arranged by size:
1542 1056 670 354 292 112 93 91
69 63 12 7
BmrI ACTGGGnnnn_n'
Cuts at: 154 754 1573 1654 1989 4133 154
Size: 600 819 81 335 2144 382
Fragments arranged by size:
2144 819 600 382 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 159 2728 159
Size: 2569 1792
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 550 571 590 1206 231
Size: 319 21 19 616 3386
Fragments arranged by size:
3386 616 319 21 19
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 263 1105 1537 2416 2908 3206 263
Size: 842 432 879 492 298 1418
Fragments arranged by size:
1418 879 842 492 432 298
BsaI GGTCTCn'nnnn_
Cuts at: 1018 4065 1018
Size: 3047 1314
BsaAI yAC'GTr
Cuts at: 1714 1714
Size: 4361
BsaBI GATnn'nnATC
Cuts at: 6 1875 3912 6
Size: 1869 2037 455
Fragments arranged by size:
2037 1869 455
BsaHI Gr'CG_yC
Cuts at: 503 1792 2040 2742 4238 503
Size: 1289 248 702 1496 626
Fragments arranged by size:
1496 1289 702 626 248
BsaWI w'CCGG_w
Cuts at: 1550 2071 2749 3023 3170 3581 1550
Size: 521 678 274 147 411 2330
Fragments arranged by size:
2330 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 916 946 1279 1309 916
Size: 30 333 30 3968
Fragments arranged by size:
3968 333 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 547 568 903 1006 547
Size: 21 335 103 3902
Fragments arranged by size:
3902 335 103 21
BseYI C'CCAG_C
Cuts at: 363 631 854 1044 1786 2544 3121 3617 363
Size: 268 223 190 742 758 577 496 1107
Fragments arranged by size:
1107 758 742 577 496 268 223 190
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 728 1149 728
Size: 421 3940
BsiEI CG_ry'CG
Cuts at: 104 1903 1949 3157 104
Size: 1799 46 1208 1308
Fragments arranged by size:
1799 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1316 1375 2153 2343 3135 1316
Size: 59 778 190 792 2542
Fragments arranged by size:
2542 792 778 190 59
BsmBI CGTCTCn'nnnn_
Cuts at: 228 228
Size: 4361
Bsp1286I G_dGCh'C
Cuts at: 316 428 720 727 739 808 899 962 1316
Size: 112 292 7 12 69 91 63 354
Cuts at: 1316 1375 1986 2079 2153 2343 2405 3135 316
Size: 59 611 93 74 190 62 730 1542
Fragments arranged by size:
1542 730 611 354 292 190 112 93
91 74 69 63 62 59 12 7
BspEI T'CCGG_A
Cuts at: 3581 3581
Size: 4361
BspHI T'CATG_A
Cuts at: 3537 3537
Size: 4361
BspMI ACCTGCnnnn'nnnn_
Cuts at: 935 1139 1411 1625 1927 2308 935
Size: 204 272 214 302 381 2988
Fragments arranged by size:
2988 381 302 272 214 204
BsrBI CCG'CTC
Cuts at: 50 1337 2653 2707 4191 4356 50
Size: 1287 1316 54 1484 165 55
Fragments arranged by size:
1484 1316 1287 165 55 54
BsrDI GCAATG_nn'
Cuts at: 947 962 2273 947
Size: 15 1311 3035
Fragments arranged by size:
3035 1311 15
BsrFI r'CCGG_y
Cuts at: 191 1009 1136 2359 2540 2749 191
Size: 818 127 1223 181 209 1803
Fragments arranged by size:
1803 1223 818 209 181 127
BsrGI T'GTAC_A
Cuts at: 1100 1100
Size: 4361
BssSI C'ACGA_G
Cuts at: 892 2632 2990 892
Size: 1740 358 2263
Fragments arranged by size:
2263 1740 358
BstAPI GCAn_nnn'nTGC
Cuts at: 715 1738 715
Size: 1023 3338
BstBI TT'CG_AA
Cuts at: 2722 2722
Size: 4361
BstYI r'GATC_y
Cuts at: 1 773 1386 1853 1876 2211 2457 3469 3555
Size: 772 613 467 23 335 246 1012 86
Cuts at: 3555 3567 3913 1
Size: 12 346 449
Fragments arranged by size:
1012 772 613 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 587 587
Size: 4361
BtgI C'CryG_G
Cuts at: 107 413 508 1324 107
Size: 306 95 816 3144
Fragments arranged by size:
3144 816 306 95
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 254 2311 2492 2655 254
Size: 2057 181 163 1960
Fragments arranged by size:
2057 1960 181 163
BtsI GCAGTG_nn'
Cuts at: 375 411 1604 375
Size: 36 1193 3132
Fragments arranged by size:
3132 1193 36
DraI TTT'AAA
Cuts at: 1360 1360
Size: 4361
DraIII CAC_nnn'GTG
Cuts at: 697 697
Size: 4361
DrdI GACnn_nn'nnGTC
Cuts at: 2067 2925 2067
Size: 858 3503
EaeI y'GGCC_r
Cuts at: 189 1171 1946 2120 2511 2538 3883 189
Size: 982 775 174 391 27 1345 667
Fragments arranged by size:
1345 982 775 667 391 174 27
EagI C'GGCC_G
Cuts at: 1946 1946
Size: 4361
EarI CTCTTCn'nnn_
Cuts at: 536 2384 2594 536
Size: 1848 210 2303
Fragments arranged by size:
2303 1848 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2569 2879 3025 4171 2569
Size: 310 146 1146 2759
Fragments arranged by size:
2759 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1373 1373
Size: 4361
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 231 248 550 571 590 1206 2186 2618 3365
Size: 17 302 21 19 616 980 432 747
Cuts at: 3365 3633 231
Size: 268 959
Fragments arranged by size:
980 959 747 616 432 302 268 21
19 17
EcoNI CCTnn'n_nnAGG
Cuts at: 432 692 432
Size: 260 4101
EcoO109I rG'GnC_Cy
Cuts at: 424 425 582 723 735 736 811 988 997
Size: 1 157 141 12 1 75 177 9
Cuts at: 997 1500 424
Size: 503 3285
Fragments arranged by size:
3285 503 177 157 141 75 12 9
1 1
EcoRI G'AATT_C
Cuts at: 1365 1365
Size: 4361
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2380 2380
Size: 4361
FspI TGC'GCA
Cuts at: 946 2142 946
Size: 1196 3165
FspAI rTGC'GCAy
Cuts at: 946 946
Size: 4361
HaeII r_GCGC'y
Cuts at: 681 1227 1795 2043 3065 4044 681
Size: 546 568 248 1022 979 998
Fragments arranged by size:
1022 998 979 568 546 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1195 1227 1279 1311 2203 2233 2235 2265 1195
Size: 32 52 32 892 30 2 30 3291
Fragments arranged by size:
3291 892 52 32 32 30 30 2
HincII GTy'rAC
Cuts at: 867 1400 867
Size: 533 3828
KasI G'GCGC_C
Cuts at: 1791 2039 1791
Size: 248 4113
KpnI G_GTAC'C
Cuts at: 910 1381 910
Size: 471 3890
MluI A'CGCG_T
Cuts at: 3794 3794
Size: 4361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 941 960 3032 3216 941
Size: 19 2072 184 2086
Fragments arranged by size:
2086 2072 184 19
MscI TGG'CCA
Cuts at: 1173 2122 1173
Size: 949 3412
MslI CAynn'nnrTG
Cuts at: 760 1063 1145 1235 1309 2350 2477 760
Size: 303 82 90 74 1041 127 2644
Fragments arranged by size:
2644 1041 303 127 90 82 74
MspA1I CmG'CkG
Cuts at: 383 442 631 668 1326 1472 1786 2146 3159
Size: 59 189 37 658 146 314 360 1013
Cuts at: 3159 3404 383
Size: 245 1340
Fragments arranged by size:
1340 1013 658 360 314 245 189 146
59 37
NaeI GCC'GGC
Cuts at: 193 1138 2542 193
Size: 945 1404 2012
Fragments arranged by size:
2012 1404 945
NarI GG'CG_CC
Cuts at: 1792 2040 1792
Size: 248 4113
NcoI C'CATG_G
Cuts at: 107 107
Size: 4361
NgoMIV G'CCGG_C
Cuts at: 191 1136 2540 191
Size: 945 1404 2012
Fragments arranged by size:
2012 1404 945
NspI r_CATG'y
Cuts at: 210 1414 1914 2821 210
Size: 1204 500 907 1750
Fragments arranged by size:
1750 1204 907 500
PasI CC'CwG_GG
Cuts at: 720 823 993 720
Size: 103 170 4088
Fragments arranged by size:
4088 170 103
PciI A'CATG_T
Cuts at: 2817 2817
Size: 4361
PflMI CCAn_nnn'nTGG
Cuts at: 697 861 697
Size: 164 4197
PmeI GTTT'AAAC
Cuts at: 1360 1360
Size: 4361
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 916 948 1258 1290 3500 3532 916
Size: 32 310 32 2210 32 1745
Fragments arranged by size:
2210 1745 310 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 582 988 582
Size: 406 3955
PspOMI G'GGCC_C
Cuts at: 424 723 735 424
Size: 299 12 4050
Fragments arranged by size:
4050 299 12
PstI C_TGCA'G
Cuts at: 619 773 1093 1408 2093 619
Size: 154 320 315 685 2887
Fragments arranged by size:
2887 685 320 315 154
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4361
PvuII CAG'CTG
Cuts at: 631 1786 2146 631
Size: 1155 360 2846
Fragments arranged by size:
2846 1155 360
RsrII CG'GwC_CG
Cuts at: 2556 2556
Size: 4361
SacI G_AGCT'C
Cuts at: 1375 1375
Size: 4361
SacII CC_GC'GG
Cuts at: 1327 1327
Size: 4361
SalI G'TCGA_C
Cuts at: 1398 1398
Size: 4361
SanDI GG'GwC_CC
Cuts at: 988 988
Size: 4361
SapI GCTCTTCn'nnn_
Cuts at: 2384 2594 2384
Size: 210 4151
SbfI CC_TGCA'GG
Cuts at: 1408 1408
Size: 4361
ScaI AGT'ACT
Cuts at: 3878 3878
Size: 4361
SexAI A'CCwGG_T
Cuts at: 693 693
Size: 4361
SfcI C'TryA_G
Cuts at: 33 615 769 1089 1404 2089 3082 3273 33
Size: 582 154 320 315 685 993 191 1121
Fragments arranged by size:
1121 993 685 582 320 315 191 154
SfoI GGC'GCC
Cuts at: 1793 2041 1793
Size: 248 4113
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4361
SmaI CCC'GGG
Cuts at: 1383 1383
Size: 4361
SmlI C'TyrA_G
Cuts at: 278 1120 1516 2431 2923 3185 278
Size: 842 396 915 492 262 1454
Fragments arranged by size:
1454 915 842 492 396 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4361
SphI G_CATG'C
Cuts at: 1414 1914 1414
Size: 500 3861
StuI AGG'CCT
Cuts at: 308 308
Size: 4361
StyI C'CwwG_G
Cuts at: 107 1495 1849 107
Size: 1388 354 2619
Fragments arranged by size:
2619 1388 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 163 478 1944 2077 2536 2745 163
Size: 315 1466 133 459 209 1779
Fragments arranged by size:
1779 1466 459 315 209 133
TatI w'GTAC_w
Cuts at: 1075 1100 3876 1075
Size: 25 2776 1560
Fragments arranged by size:
2776 1560 25
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 500 532 3878 3910 500
Size: 32 3346 32 951
Fragments arranged by size:
3346 951 32 32
Tth111I GACn'n_nGTC
Cuts at: 2158 2158
Size: 4361
XbaI T'CTAG_A
Cuts at: 1392 1392
Size: 4361
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 628 655 727 1181 1856 628
Size: 27 72 454 675 3133
Fragments arranged by size:
3133 675 454 72 27
XmaI C'CCGG_G
Cuts at: 1381 1381
Size: 4361
XmnI GAAnn'nnTTC
Cuts at: 545 1156 1273 545
Size: 611 117 3633
Fragments arranged by size:
3633 611 117
ZraI GAC'GTC
Cuts at: 504 504
Size: 4361
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI
BclI BglII BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI FspI FspAI HaeII Hin4I HincII KasI KpnI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PasI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AflII AlfI AscI AvrII BaeI BbsI BbvCI BcgI
BfrBI BglI BmgBI BmtI Bpu10I BsiWI BsmI BssHII
BstEII BstXI BstZ17I ClaI CspCI EcoRV FseI HindIII
HpaI MfeI NdeI NheI NotI NruI NsiI PacI
PfoI PmlI PshAI PsiI PspXI PsrI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI