Restriction map for
pF1KB7701
(Circular) (Six-base) MAPSORT of: pF1KB7701.seq Check: 2630 from: 1 to: 4355
pF1KB7701 4355 bp
With 182 enzymes: SgfI *
October 16, 2009 16:19 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 723 723
Size: 4355
AccI GT'mk_AC
Cuts at: 1393 1393
Size: 4355
Acc65I G'GTAC_C
Cuts at: 1371 1371
Size: 4355
AclI AA'CG_TT
Cuts at: 4195 4195
Size: 4355
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2180 2612 3359 3627 2180
Size: 432 747 268 2908
Fragments arranged by size:
2908 747 432 268
AflIII A'CryG_T
Cuts at: 1224 1707 2811 3788 1224
Size: 483 1104 977 1791
Fragments arranged by size:
1791 1104 977 483
AgeI A'CCGG_T
Cuts at: 2743 2743
Size: 4355
AleI CACnn'nnGTG
Cuts at: 226 1085 1225 226
Size: 859 140 3356
Fragments arranged by size:
3356 859 140
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1243 1243
Size: 4355
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 745 777 745
Size: 32 4323
AlwNI CAG_nnn'CTG
Cuts at: 372 1660 3227 372
Size: 1288 1567 1500
Fragments arranged by size:
1567 1500 1288
ApaLI G'TGCA_C
Cuts at: 397 1170 3125 397
Size: 773 1955 1627
Fragments arranged by size:
1955 1627 773
ApoI r'AATT_y
Cuts at: 940 1359 3708 3964 940
Size: 419 2349 256 1331
Fragments arranged by size:
2349 1331 419 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4355
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
AvaI C'yCGr_G
Cuts at: 114 705 1375 114
Size: 591 670 3094
Fragments arranged by size:
3094 670 591
BamHI G'GATC_C
Cuts at: 1380 1380
Size: 4355
BanI G'GyrC_C
Cuts at: 510 697 1371 1785 2033 2068 510
Size: 187 674 414 248 35 2797
Fragments arranged by size:
2797 674 414 248 187 35
BanII G_rGCy'C
Cuts at: 1369 2399 1369
Size: 1030 3325
BbeI G_GCGC'C
Cuts at: 701 1789 2037 701
Size: 1088 248 3019
Fragments arranged by size:
3019 1088 248
BbvCI CC'TCA_GC
Cuts at: 664 664
Size: 4355
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 378 412 378
Size: 34 4321
BciVI GTATCCnnnnn_n'
Cuts at: 2253 3014 4355 2253
Size: 761 1341 2253
Fragments arranged by size:
2253 1341 761
BclI T'GATC_A
Cuts at: 1875 1875
Size: 4355
BglI GCCn_nnn'nGGC
Cuts at: 682 682
Size: 4355
BglII A'GATC_T
Cuts at: 1870 3907 1870
Size: 2037 2318
BlpI GC'TnA_GC
Cuts at: 1467 1467
Size: 4355
Bme1580I G_kGCm'C
Cuts at: 308 401 515 1174 1264 1980 2073 3129 308
Size: 93 114 659 90 716 93 1056 1534
Fragments arranged by size:
1534 1056 716 659 114 93 93 90
BmgBI CAC'GTC
Cuts at: 1083 1083
Size: 4355
BmrI ACTGGGnnnn_n'
Cuts at: 1567 1648 1983 4127 1567
Size: 81 335 2144 1795
Fragments arranged by size:
2144 1795 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2722 2722
Size: 4355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 914 1172 772
Size: 142 258 3955
Fragments arranged by size:
3955 258 142
Bpu10I CC'TnA_GC
Cuts at: 664 1331 664
Size: 667 3688
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1531 2410 2902 3200 1531
Size: 879 492 298 2686
Fragments arranged by size:
2686 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 187 202 211 4059 187
Size: 15 9 3848 483
Fragments arranged by size:
3848 483 15 9
BsaAI yAC'GTr
Cuts at: 1708 1708
Size: 4355
BsaBI GATnn'nnATC
Cuts at: 6 502 1869 3906 6
Size: 496 1367 2037 455
Fragments arranged by size:
2037 1367 496 455
BsaHI Gr'CG_yC
Cuts at: 698 1786 2034 2736 4232 698
Size: 1088 248 702 1496 821
Fragments arranged by size:
1496 1088 821 702 248
BsaWI w'CCGG_w
Cuts at: 1240 1544 2065 2743 3017 3164 3575 1240
Size: 304 521 678 274 147 411 2020
Fragments arranged by size:
2020 678 521 411 304 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 745 775 745
Size: 30 4325
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 250 253 256 259 451 250
Size: 3 3 3 192 4154
Fragments arranged by size:
4154 192 3 3 3
BseYI C'CCAG_C
Cuts at: 1780 2538 3115 3611 1780
Size: 758 577 496 2524
Fragments arranged by size:
2524 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 160 445 757 999 1042 1109 160
Size: 285 312 242 43 67 3406
Fragments arranged by size:
3406 312 285 242 67 43
BsiEI CG_ry'CG
Cuts at: 104 161 524 575 618 1897 1943 3151 104
Size: 57 363 51 43 1279 46 1208 1308
Fragments arranged by size:
1308 1279 1208 363 57 51 46 43
BsiHKAI G_wGCw'C
Cuts at: 401 1174 1369 2147 2337 3129 401
Size: 773 195 778 190 792 1627
Fragments arranged by size:
1627 792 778 773 195 190
Bsp1286I G_dGCh'C
Cuts at: 308 401 515 1174 1264 1369 1980 2073 2147
Size: 93 114 659 90 105 611 93 74
Cuts at: 2147 2337 2399 3129 308
Size: 190 62 730 1534
Fragments arranged by size:
1534 730 659 611 190 114 105 93
93 90 74 62
BspEI T'CCGG_A
Cuts at: 3575 3575
Size: 4355
BspHI T'CATG_A
Cuts at: 3531 3531
Size: 4355
BspMI ACCTGCnnnn'nnnn_
Cuts at: 723 1405 1619 1921 2302 723
Size: 682 214 302 381 2776
Fragments arranged by size:
2776 682 381 302 214
BsrBI CCG'CTC
Cuts at: 50 584 647 2647 2701 4185 4350 50
Size: 534 63 2000 54 1484 165 55
Fragments arranged by size:
2000 1484 534 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2267 2267
Size: 4355
BsrFI r'CCGG_y
Cuts at: 156 310 519 523 574 676 2353 2534 2743
Size: 154 209 4 51 102 1677 181 209
Cuts at: 2743 156
Size: 1768
Fragments arranged by size:
1768 1677 209 209 181 154 102 51
4
BssHII G'CGCG_C
Cuts at: 471 471
Size: 4355
BssSI C'ACGA_G
Cuts at: 178 2626 2984 178
Size: 2448 358 1549
Fragments arranged by size:
2448 1549 358
BstAPI GCAn_nnn'nTGC
Cuts at: 943 1732 943
Size: 789 3566
BstBI TT'CG_AA
Cuts at: 1178 2716 1178
Size: 1538 2817
BstEII G'GTnAC_C
Cuts at: 375 558 768 375
Size: 183 210 3962
Fragments arranged by size:
3962 210 183
BstXI CCAn_nnnn'nTGG
Cuts at: 204 228 933 204
Size: 24 705 3626
Fragments arranged by size:
3626 705 24
BstYI r'GATC_y
Cuts at: 1 921 1380 1847 1870 2205 2451 3463 3549
Size: 920 459 467 23 335 246 1012 86
Cuts at: 3549 3561 3907 1
Size: 12 346 449
Fragments arranged by size:
1012 920 467 459 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 107 137 282 300 563 1065 107
Size: 30 145 18 263 502 3397
Fragments arranged by size:
3397 502 263 145 30 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 117 2305 2486 2649 117
Size: 2188 181 163 1823
Fragments arranged by size:
2188 1823 181 163
BtsI GCAGTG_nn'
Cuts at: 1598 1598
Size: 4355
DraI TTT'AAA
Cuts at: 1354 1354
Size: 4355
DraIII CAC_nnn'GTG
Cuts at: 776 776
Size: 4355
DrdI GACnn_nn'nnGTC
Cuts at: 2061 2919 2061
Size: 858 3497
EaeI y'GGCC_r
Cuts at: 158 297 312 521 572 615 1040 1940 2114
Size: 139 15 209 51 43 425 900 174
Cuts at: 2114 2505 2532 3877 158
Size: 391 27 1345 636
Fragments arranged by size:
1345 900 636 425 391 209 174 139
51 43 27 15
EagI C'GGCC_G
Cuts at: 158 521 572 615 1940 158
Size: 363 51 43 1325 2573
Fragments arranged by size:
2573 1325 363 51 43
EarI CTCTTCn'nnn_
Cuts at: 637 649 2378 2588 637
Size: 12 1729 210 2404
Fragments arranged by size:
2404 1729 210 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2563 2873 3019 4165 2563
Size: 310 146 1146 2753
Fragments arranged by size:
2753 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1367 1367
Size: 4355
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 772 914 1172 2180 2612 3359 3627 772
Size: 142 258 1008 432 747 268 1500
Fragments arranged by size:
1500 1008 747 432 268 258 142
EcoNI CCTnn'n_nnAGG
Cuts at: 893 1007 893
Size: 114 4241
EcoO109I rG'GnC_Cy
Cuts at: 1250 1494 1250
Size: 244 4111
EcoRI G'AATT_C
Cuts at: 1359 1359
Size: 4355
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2374 2374
Size: 4355
FspI TGC'GCA
Cuts at: 1219 2136 1219
Size: 917 3438
HaeII r_GCGC'y
Cuts at: 701 1789 2037 3059 4038 701
Size: 1088 248 1022 979 1018
Fragments arranged by size:
1088 1022 1018 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1038 1067 1070 1099 2197 2227 2229 2259 1038
Size: 29 3 29 1098 30 2 30 3134
Fragments arranged by size:
3134 1098 30 30 29 29 3 2
HincII GTy'rAC
Cuts at: 1298 1394 1298
Size: 96 4259
HindIII A'AGCT_T
Cuts at: 1103 1103
Size: 4355
KasI G'GCGC_C
Cuts at: 697 1785 2033 697
Size: 1088 248 3019
Fragments arranged by size:
3019 1088 248
KpnI G_GTAC'C
Cuts at: 1375 1375
Size: 4355
MluI A'CGCG_T
Cuts at: 3788 3788
Size: 4355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 3026 3210 3026
Size: 184 4171
MscI TGG'CCA
Cuts at: 2116 2116
Size: 4355
MslI CAynn'nnrTG
Cuts at: 202 226 508 1021 1085 1225 2344 2471 202
Size: 24 282 513 64 140 1119 127 2086
Fragments arranged by size:
2086 1119 513 282 140 127 64 24
MspA1I CmG'CkG
Cuts at: 372 668 1466 1780 2140 3153 3398 372
Size: 296 798 314 360 1013 245 1329
Fragments arranged by size:
1329 1013 798 360 314 296 245
NaeI GCC'GGC
Cuts at: 158 312 521 525 576 678 2536 158
Size: 154 209 4 51 102 1858 1977
Fragments arranged by size:
1977 1858 209 154 102 51 4
NarI GG'CG_CC
Cuts at: 698 1786 2034 698
Size: 1088 248 3019
Fragments arranged by size:
3019 1088 248
NcoI C'CATG_G
Cuts at: 107 107
Size: 4355
NgoMIV G'CCGG_C
Cuts at: 156 310 519 523 574 676 2534 156
Size: 154 209 4 51 102 1858 1977
Fragments arranged by size:
1977 1858 209 154 102 51 4
NotI GC'GGCC_GC
Cuts at: 615 615
Size: 4355
NspI r_CATG'y
Cuts at: 1228 1330 1408 1908 2815 1228
Size: 102 78 500 907 2768
Fragments arranged by size:
2768 907 500 102 78
PciI A'CATG_T
Cuts at: 1224 2811 1224
Size: 1587 2768
PfoI T'CCnGG_A
Cuts at: 891 891
Size: 4355
PmeI GTTT'AAAC
Cuts at: 1354 1354
Size: 4355
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 745 777 3494 3526 745
Size: 32 2717 32 1574
Fragments arranged by size:
2717 1574 32 32
PstI C_TGCA'G
Cuts at: 368 1402 2087 368
Size: 1034 685 2636
Fragments arranged by size:
2636 1034 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4355
PvuII CAG'CTG
Cuts at: 1780 2140 1780
Size: 360 3995
RsrII CG'GwC_CG
Cuts at: 2550 2550
Size: 4355
SacI G_AGCT'C
Cuts at: 1369 1369
Size: 4355
SalI G'TCGA_C
Cuts at: 1392 1392
Size: 4355
SapI GCTCTTCn'nnn_
Cuts at: 637 2378 2588 637
Size: 1741 210 2404
Fragments arranged by size:
2404 1741 210
SbfI CC_TGCA'GG
Cuts at: 1402 1402
Size: 4355
ScaI AGT'ACT
Cuts at: 3872 3872
Size: 4355
SexAI A'CCwGG_T
Cuts at: 1346 1346
Size: 4355
SfcI C'TryA_G
Cuts at: 33 364 1398 2083 3076 3267 33
Size: 331 1034 685 993 191 1121
Fragments arranged by size:
1121 1034 993 685 331 191
SfoI GGC'GCC
Cuts at: 699 1787 2035 699
Size: 1088 248 3019
Fragments arranged by size:
3019 1088 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4355
SmaI CCC'GGG
Cuts at: 707 1377 707
Size: 670 3685
SmlI C'TyrA_G
Cuts at: 1510 2425 2917 3179 1510
Size: 915 492 262 2686
Fragments arranged by size:
2686 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4355
SphI G_CATG'C
Cuts at: 1408 1908 1408
Size: 500 3855
StyI C'CwwG_G
Cuts at: 107 1489 1843 107
Size: 1382 354 2619
Fragments arranged by size:
2619 1382 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 756 1078 1938 2071 2530 2739 756
Size: 322 860 133 459 209 2372
Fragments arranged by size:
2372 860 459 322 209 133
TatI w'GTAC_w
Cuts at: 3870 3870
Size: 4355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1053 1085 3872 3904 1053
Size: 32 2787 32 1504
Fragments arranged by size:
2787 1504 32 32
Tth111I GACn'n_nGTC
Cuts at: 2152 2152
Size: 4355
XbaI T'CTAG_A
Cuts at: 1386 1386
Size: 4355
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1850 1850
Size: 4355
XmaI C'CCGG_G
Cuts at: 705 1375 705
Size: 670 3685
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BtgI BtgZI BtsI DraI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PfoI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI ApaI AscI AvrII BaeI
BbsI BfrBI BmtI BsiWI BsmI BsmBI BsrGI BstZ17I
Bsu36I ClaI CspCI EcoRV FseI FspAI HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PflMI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI XhoI
XmnI ZraI