Restriction map for
pF1KB7688
(Circular) (Six-base) MAPSORT of: pF1KB7688.seq Check: 6468 from: 1 to: 4343
pF1KB7688 4343 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 890 890
Size: 4343
AccI GT'mk_AC
Cuts at: 614 1381 614
Size: 767 3576
Acc65I G'GTAC_C
Cuts at: 1359 1359
Size: 4343
AclI AA'CG_TT
Cuts at: 4183 4183
Size: 4343
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 2168 2600 3347 3615 1027
Size: 1141 432 747 268 1755
Fragments arranged by size:
1755 1141 747 432 268
AflIII A'CryG_T
Cuts at: 1695 2799 3776 1695
Size: 1104 977 2262
Fragments arranged by size:
2262 1104 977
AgeI A'CCGG_T
Cuts at: 2731 2731
Size: 4343
AhdI GACnn_n'nnGTC
Cuts at: 610 610
Size: 4343
AleI CACnn'nnGTG
Cuts at: 334 334
Size: 4343
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 373 405 637 669 373
Size: 32 232 32 4047
Fragments arranged by size:
4047 232 32 32
AlwNI CAG_nnn'CTG
Cuts at: 1648 3215 1648
Size: 1567 2776
ApaLI G'TGCA_C
Cuts at: 3113 3113
Size: 4343
ApoI r'AATT_y
Cuts at: 946 1347 3696 3952 946
Size: 401 2349 256 1337
Fragments arranged by size:
2349 1337 401 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4343
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
AvaI C'yCGr_G
Cuts at: 178 232 1363 178
Size: 54 1131 3158
Fragments arranged by size:
3158 1131 54
BamHI G'GATC_C
Cuts at: 1368 1368
Size: 4343
BanI G'GyrC_C
Cuts at: 1359 1773 2021 2056 1359
Size: 414 248 35 3646
Fragments arranged by size:
3646 414 248 35
BanII G_rGCy'C
Cuts at: 1357 2387 1357
Size: 1030 3313
BbeI G_GCGC'C
Cuts at: 1777 2025 1777
Size: 248 4095
BbsI GAAGACnn'nnnn_
Cuts at: 147 919 1064 147
Size: 772 145 3426
Fragments arranged by size:
3426 772 145
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 517 551 517
Size: 34 4309
BciVI GTATCCnnnnn_n'
Cuts at: 2241 3002 4343 2241
Size: 761 1341 2241
Fragments arranged by size:
2241 1341 761
BclI T'GATC_A
Cuts at: 1863 1863
Size: 4343
BglII A'GATC_T
Cuts at: 1858 3895 1858
Size: 2037 2306
BlpI GC'TnA_GC
Cuts at: 1455 1455
Size: 4343
Bme1580I G_kGCm'C
Cuts at: 1968 2061 3117 1968
Size: 93 1056 3194
Fragments arranged by size:
3194 1056 93
BmgBI CAC'GTC
Cuts at: 1166 1166
Size: 4343
BmrI ACTGGGnnnn_n'
Cuts at: 1555 1636 1971 4115 1555
Size: 81 335 2144 1783
Fragments arranged by size:
2144 1783 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2710 2710
Size: 4343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1054 1117 1242 1054
Size: 63 125 4155
Fragments arranged by size:
4155 125 63
Bpu10I CC'TnA_GC
Cuts at: 704 1121 704
Size: 417 3926
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 133 1519 2398 2890 3188 133
Size: 1386 879 492 298 1288
Fragments arranged by size:
1386 1288 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 306 1247 4047 306
Size: 941 2800 602
Fragments arranged by size:
2800 941 602
BsaAI yAC'GTr
Cuts at: 1107 1696 1107
Size: 589 3754
BsaBI GATnn'nnATC
Cuts at: 6 1857 3894 6
Size: 1851 2037 455
Fragments arranged by size:
2037 1851 455
BsaHI Gr'CG_yC
Cuts at: 1774 2022 2724 4220 1774
Size: 248 702 1496 1897
Fragments arranged by size:
1897 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1272 1532 2053 2731 3005 3152 3563 1272
Size: 260 521 678 274 147 411 2052
Fragments arranged by size:
2052 678 521 411 274 260 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 637 661 667 691 1240 1270 637
Size: 24 6 24 549 30 3710
Fragments arranged by size:
3710 549 30 24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 387 753 1315 387
Size: 366 562 3415
Fragments arranged by size:
3415 562 366
BseYI C'CCAG_C
Cuts at: 284 358 1768 2526 3103 3599 284
Size: 74 1410 758 577 496 1028
Fragments arranged by size:
1410 1028 758 577 496 74
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 508 838 1229 508
Size: 330 391 3622
Fragments arranged by size:
3622 391 330
BsiEI CG_ry'CG
Cuts at: 104 1885 1931 3139 104
Size: 1781 46 1208 1308
Fragments arranged by size:
1781 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1357 2135 2325 3117 1357
Size: 778 190 792 2583
Fragments arranged by size:
2583 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1357 1968 2061 2135 2325 2387 3117 1357
Size: 611 93 74 190 62 730 2583
Fragments arranged by size:
2583 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3563 3563
Size: 4343
BspHI T'CATG_A
Cuts at: 3519 3519
Size: 4343
BspMI ACCTGCnnnn'nnnn_
Cuts at: 890 1147 1393 1607 1909 2290 890
Size: 257 246 214 302 381 2943
Fragments arranged by size:
2943 381 302 257 246 214
BsrBI CCG'CTC
Cuts at: 50 231 2635 2689 4173 4338 50
Size: 181 2404 54 1484 165 55
Fragments arranged by size:
2404 1484 181 165 55 54
BsrDI GCAATG_nn'
Cuts at: 278 2255 278
Size: 1977 2366
BsrFI r'CCGG_y
Cuts at: 121 315 827 851 2341 2522 2731 121
Size: 194 512 24 1490 181 209 1733
Fragments arranged by size:
1733 1490 512 209 194 181 24
BssSI C'ACGA_G
Cuts at: 199 2614 2972 199
Size: 2415 358 1570
Fragments arranged by size:
2415 1570 358
BstAPI GCAn_nnn'nTGC
Cuts at: 118 246 1025 1720 118
Size: 128 779 695 2741
Fragments arranged by size:
2741 779 695 128
BstBI TT'CG_AA
Cuts at: 2704 2704
Size: 4343
BstXI CCAn_nnnn'nTGG
Cuts at: 1080 1095 1080
Size: 15 4328
BstYI r'GATC_y
Cuts at: 1 1368 1835 1858 2193 2439 3451 3537 3549
Size: 1367 467 23 335 246 1012 86 12
Cuts at: 3549 3895 1
Size: 346 449
Fragments arranged by size:
1367 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4343
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2293 2474 2637 2293
Size: 181 163 3999
Fragments arranged by size:
3999 181 163
BtsI GCAGTG_nn'
Cuts at: 1586 1586
Size: 4343
DraI TTT'AAA
Cuts at: 1342 1342
Size: 4343
DrdI GACnn_nn'nnGTC
Cuts at: 1275 2049 2907 1275
Size: 774 858 2711
Fragments arranged by size:
2711 858 774
EaeI y'GGCC_r
Cuts at: 203 638 853 1070 1223 1928 2102 2493 2520
Size: 435 215 217 153 705 174 391 27
Cuts at: 2520 3865 203
Size: 1345 681
Fragments arranged by size:
1345 705 681 435 391 217 215 174
153 27
EagI C'GGCC_G
Cuts at: 1928 1928
Size: 4343
EarI CTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2551 2861 3007 4153 2551
Size: 310 146 1146 2741
Fragments arranged by size:
2741 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1355 1355
Size: 4343
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1027 1054 1117 1242 2168 2600 3347 3615 1027
Size: 27 63 125 926 432 747 268 1755
Fragments arranged by size:
1755 926 747 432 268 125 63 27
EcoO109I rG'GnC_Cy
Cuts at: 450 1482 450
Size: 1032 3311
EcoRI G'AATT_C
Cuts at: 1347 1347
Size: 4343
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 261 2362 261
Size: 2101 2242
FspI TGC'GCA
Cuts at: 2124 2124
Size: 4343
HaeII r_GCGC'y
Cuts at: 1777 2025 3047 4026 1777
Size: 248 1022 979 2094
Fragments arranged by size:
2094 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 596 628 2185 2215 2217 2247 596
Size: 32 1557 30 2 30 2692
Fragments arranged by size:
2692 1557 32 30 30 2
HincII GTy'rAC
Cuts at: 225 276 900 1382 225
Size: 51 624 482 3186
Fragments arranged by size:
3186 624 482 51
KasI G'GCGC_C
Cuts at: 1773 2021 1773
Size: 248 4095
KpnI G_GTAC'C
Cuts at: 1363 1363
Size: 4343
MluI A'CGCG_T
Cuts at: 3776 3776
Size: 4343
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 416 1113 3014 3198 416
Size: 697 1901 184 1561
Fragments arranged by size:
1901 1561 697 184
MscI TGG'CCA
Cuts at: 640 1072 1225 2104 640
Size: 432 153 879 2879
Fragments arranged by size:
2879 879 432 153
MslI CAynn'nnrTG
Cuts at: 334 1000 2332 2459 334
Size: 666 1332 127 2218
Fragments arranged by size:
2218 1332 666 127
MspA1I CmG'CkG
Cuts at: 1253 1454 1768 2128 3141 3386 1253
Size: 201 314 360 1013 245 2210
Fragments arranged by size:
2210 1013 360 314 245 201
NaeI GCC'GGC
Cuts at: 853 2524 853
Size: 1671 2672
NarI GG'CG_CC
Cuts at: 1774 2022 1774
Size: 248 4095
NcoI C'CATG_G
Cuts at: 107 107
Size: 4343
NgoMIV G'CCGG_C
Cuts at: 851 2522 851
Size: 1671 2672
NspI r_CATG'y
Cuts at: 835 1396 1896 2803 835
Size: 561 500 907 2375
Fragments arranged by size:
2375 907 561 500
PciI A'CATG_T
Cuts at: 2799 2799
Size: 4343
PflMI CCAn_nnn'nTGG
Cuts at: 291 291
Size: 4343
PmeI GTTT'AAAC
Cuts at: 1342 1342
Size: 4343
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 637 669 3482 3514 637
Size: 32 2813 32 1466
Fragments arranged by size:
2813 1466 32 32
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 773 805 773
Size: 32 4311
PstI C_TGCA'G
Cuts at: 931 1390 2075 931
Size: 459 685 3199
Fragments arranged by size:
3199 685 459
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4343
PvuII CAG'CTG
Cuts at: 1768 2128 1768
Size: 360 3983
RsrII CG'GwC_CG
Cuts at: 2538 2538
Size: 4343
SacI G_AGCT'C
Cuts at: 1357 1357
Size: 4343
SalI G'TCGA_C
Cuts at: 1380 1380
Size: 4343
SapI GCTCTTCn'nnn_
Cuts at: 2366 2576 2366
Size: 210 4133
SbfI CC_TGCA'GG
Cuts at: 1390 1390
Size: 4343
ScaI AGT'ACT
Cuts at: 3860 3860
Size: 4343
SexAI A'CCwGG_T
Cuts at: 812 812
Size: 4343
SfcI C'TryA_G
Cuts at: 33 790 927 1386 2071 3064 3255 33
Size: 757 137 459 685 993 191 1121
Fragments arranged by size:
1121 993 757 685 459 191 137
SfoI GGC'GCC
Cuts at: 1775 2023 1775
Size: 248 4095
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4343
SmaI CCC'GGG
Cuts at: 180 1365 180
Size: 1185 3158
SmlI C'TyrA_G
Cuts at: 148 1498 2413 2905 3167 148
Size: 1350 915 492 262 1324
Fragments arranged by size:
1350 1324 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4343
SphI G_CATG'C
Cuts at: 835 1396 1896 835
Size: 561 500 3282
Fragments arranged by size:
3282 561 500
StyI C'CwwG_G
Cuts at: 107 403 656 1088 1477 1831 107
Size: 296 253 432 389 354 2619
Fragments arranged by size:
2619 432 389 354 296 253
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 879 1926 2059 2518 2727 879
Size: 1047 133 459 209 2495
Fragments arranged by size:
2495 1047 459 209 133
TatI w'GTAC_w
Cuts at: 1247 3858 1247
Size: 2611 1732
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 661 693 3860 3892 661
Size: 32 3167 32 1112
Fragments arranged by size:
3167 1112 32 32
Tth111I GACn'n_nGTC
Cuts at: 2140 2140
Size: 4343
XbaI T'CTAG_A
Cuts at: 1374 1374
Size: 4343
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1838 1838
Size: 4343
XmaI C'CCGG_G
Cuts at: 178 1363 178
Size: 1185 3158
XmnI GAAnn'nnTTC
Cuts at: 785 785
Size: 4343
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AhdI
AleI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI
PmeI PpiI PsrI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AlfI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmtI BsiWI BsmI BsmBI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI SacII SanDI SfiI SgrAI
SnaBI SrfI SspI StuI SwaI XhoI ZraI