Restriction map for
pF1KB7682
(Circular) (Six-base) MAPSORT of: pF1KB7682.seq Check: 9581 from: 1 to: 4331
pF1KB7682 4331 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 1039 912
Size: 127 4204
AccI GT'mk_AC
Cuts at: 498 1280 1369 498
Size: 782 89 3460
Fragments arranged by size:
3460 782 89
Acc65I G'GTAC_C
Cuts at: 447 1347 447
Size: 900 3431
AclI AA'CG_TT
Cuts at: 4171 4171
Size: 4331
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1241 2156 2588 3335 3603 1241
Size: 915 432 747 268 1969
Fragments arranged by size:
1969 915 747 432 268
AflIII A'CryG_T
Cuts at: 1683 2787 3764 1683
Size: 1104 977 2250
Fragments arranged by size:
2250 1104 977
AgeI A'CCGG_T
Cuts at: 2719 2719
Size: 4331
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 418 418
Size: 4331
AlwNI CAG_nnn'CTG
Cuts at: 618 1636 3203 618
Size: 1018 1567 1746
Fragments arranged by size:
1746 1567 1018
ApaI G_GGCC'C
Cuts at: 265 484 535 664 730 1067 265
Size: 219 51 129 66 337 3529
Fragments arranged by size:
3529 337 219 129 66 51
ApaLI G'TGCA_C
Cuts at: 1179 3101 1179
Size: 1922 2409
ApoI r'AATT_y
Cuts at: 1335 3684 3940 1335
Size: 2349 256 1726
Fragments arranged by size:
2349 1726 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4331
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
AvaI C'yCGr_G
Cuts at: 1351 1351
Size: 4331
BamHI G'GATC_C
Cuts at: 1356 1356
Size: 4331
BanI G'GyrC_C
Cuts at: 120 447 1032 1099 1347 1761 2009 2044 120
Size: 327 585 67 248 414 248 35 2407
Fragments arranged by size:
2407 585 414 327 248 248 67 35
BanII G_rGCy'C
Cuts at: 265 360 484 535 664 730 768 809 932
Size: 95 124 51 129 66 38 41 123
Cuts at: 932 1060 1067 1345 2375 265
Size: 128 7 278 1030 2221
Fragments arranged by size:
2221 1030 278 129 128 124 123 95
66 51 41 38 7
BbeI G_GCGC'C
Cuts at: 124 1765 2013 124
Size: 1641 248 2442
Fragments arranged by size:
2442 1641 248
BciVI GTATCCnnnnn_n'
Cuts at: 2229 2990 4331 2229
Size: 761 1341 2229
Fragments arranged by size:
2229 1341 761
BclI T'GATC_A
Cuts at: 1851 1851
Size: 4331
BglI GCCn_nnn'nGGC
Cuts at: 118 1237 118
Size: 1119 3212
BglII A'GATC_T
Cuts at: 1846 3883 1846
Size: 2037 2294
BlpI GC'TnA_GC
Cuts at: 755 1443 755
Size: 688 3643
Bme1580I G_kGCm'C
Cuts at: 265 407 484 535 664 730 1012 1067 1183
Size: 142 77 51 129 66 282 55 116
Cuts at: 1183 1956 2049 3105 265
Size: 773 93 1056 1491
Fragments arranged by size:
1491 1056 773 282 142 129 116 93
77 66 55 51
BmrI ACTGGGnnnn_n'
Cuts at: 635 658 968 1543 1624 1959 4103 635
Size: 23 310 575 81 335 2144 863
Fragments arranged by size:
2144 863 575 335 310 81 23
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 951 2698 951
Size: 1747 2584
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 488 772 998 163
Size: 325 284 226 3496
Fragments arranged by size:
3496 325 284 226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1240 1507 2386 2878 3176 1240
Size: 267 879 492 298 2395
Fragments arranged by size:
2395 879 492 298 267
BsaI GGTCTCn'nnnn_
Cuts at: 786 4035 786
Size: 3249 1082
BsaAI yAC'GTr
Cuts at: 1684 1684
Size: 4331
BsaBI GATnn'nnATC
Cuts at: 6 1845 3882 6
Size: 1839 2037 455
Fragments arranged by size:
2037 1839 455
BsaHI Gr'CG_yC
Cuts at: 121 1762 2010 2712 4208 121
Size: 1641 248 702 1496 244
Fragments arranged by size:
1641 1496 702 248 244
BsaWI w'CCGG_w
Cuts at: 132 846 1520 2041 2719 2993 3140 3551 132
Size: 714 674 521 678 274 147 411 912
Fragments arranged by size:
912 714 678 674 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 925 955 1258 1288 925
Size: 30 303 30 3968
Fragments arranged by size:
3968 303 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 773 773
Size: 4331
BseYI C'CCAG_C
Cuts at: 181 485 595 723 811 1067 1756 2514 3091
Size: 304 110 128 88 256 689 758 577
Cuts at: 3091 3587 181
Size: 496 925
Fragments arranged by size:
925 758 689 577 496 304 256 128
110 88
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 445 445
Size: 4331
BsiEI CG_ry'CG
Cuts at: 104 1873 1919 3127 104
Size: 1769 46 1208 1308
Fragments arranged by size:
1769 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1183 1345 2123 2313 3105 1183
Size: 162 778 190 792 2409
Fragments arranged by size:
2409 792 778 190 162
Bsp1286I G_dGCh'C
Cuts at: 265 360 407 484 535 664 730 768 809
Size: 95 47 77 51 129 66 38 41
Cuts at: 809 932 1012 1060 1067 1183 1345 1956 2049
Size: 123 80 48 7 116 162 611 93
Cuts at: 2049 2123 2313 2375 3105 265
Size: 74 190 62 730 1491
Fragments arranged by size:
1491 730 611 190 162 129 123 116
95 93 80 77 74 66 62 51
48 47 41 38 7
BspEI T'CCGG_A
Cuts at: 846 3551 846
Size: 2705 1626
BspHI T'CATG_A
Cuts at: 3507 3507
Size: 4331
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1039 1381 1595 1897 2278 912
Size: 127 342 214 302 381 2965
Fragments arranged by size:
2965 381 342 302 214 127
BsrBI CCG'CTC
Cuts at: 50 2623 2677 4161 4326 50
Size: 2573 54 1484 165 55
Fragments arranged by size:
2573 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2243 2243
Size: 4331
BsrFI r'CCGG_y
Cuts at: 208 2329 2510 2719 208
Size: 2121 181 209 1820
Fragments arranged by size:
2121 1820 209 181
BssSI C'ACGA_G
Cuts at: 2602 2960 2602
Size: 358 3973
BstAPI GCAn_nnn'nTGC
Cuts at: 654 1708 654
Size: 1054 3277
BstBI TT'CG_AA
Cuts at: 2692 2692
Size: 4331
BstXI CCAn_nnnn'nTGG
Cuts at: 528 562 796 1029 528
Size: 34 234 233 3830
Fragments arranged by size:
3830 234 233 34
BstYI r'GATC_y
Cuts at: 1 1268 1356 1823 1846 2181 2427 3439 3525
Size: 1267 88 467 23 335 246 1012 86
Cuts at: 3525 3537 3883 1
Size: 12 346 449
Fragments arranged by size:
1267 1012 467 449 346 335 246 88
86 23 12
BtgI C'CryG_G
Cuts at: 107 308 698 107
Size: 201 390 3740
Fragments arranged by size:
3740 390 201
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2281 2462 2625 2281
Size: 181 163 3987
Fragments arranged by size:
3987 181 163
BtsI GCAGTG_nn'
Cuts at: 999 1574 999
Size: 575 3756
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 1032 1067 1032
Size: 35 4296
DraI TTT'AAA
Cuts at: 1330 1330
Size: 4331
DrdI GACnn_nn'nnGTC
Cuts at: 2037 2895 2037
Size: 858 3473
EaeI y'GGCC_r
Cuts at: 110 1916 2090 2481 2508 3853 110
Size: 1806 174 391 27 1345 588
Fragments arranged by size:
1806 1345 588 391 174 27
EagI C'GGCC_G
Cuts at: 1916 1916
Size: 4331
EarI CTCTTCn'nnn_
Cuts at: 217 2354 2564 217
Size: 2137 210 1984
Fragments arranged by size:
2137 1984 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2539 2849 2995 4141 2539
Size: 310 146 1146 2729
Fragments arranged by size:
2729 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1343 1343
Size: 4331
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 163 488 772 998 1241 2156 2588 3335 3603
Size: 325 284 226 243 915 432 747 268
Cuts at: 3603 163
Size: 891
Fragments arranged by size:
915 891 747 432 325 284 268 243
226
EcoNI CCTnn'n_nnAGG
Cuts at: 1076 1076
Size: 4331
EcoO109I rG'GnC_Cy
Cuts at: 261 262 480 481 532 559 1064 1470 261
Size: 1 218 1 51 27 505 406 3122
Fragments arranged by size:
3122 505 406 218 51 27 1 1
EcoRI G'AATT_C
Cuts at: 1335 1335
Size: 4331
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2350 2350
Size: 4331
FspI TGC'GCA
Cuts at: 2112 2112
Size: 4331
HaeII r_GCGC'y
Cuts at: 124 1202 1765 2013 3035 4014 124
Size: 1078 563 248 1022 979 441
Fragments arranged by size:
1078 1022 979 563 441 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 783 815 1258 1290 2173 2203 2205 2235 783
Size: 32 443 32 883 30 2 30 2879
Fragments arranged by size:
2879 883 443 32 32 30 30 2
HincII GTy'rAC
Cuts at: 499 1370 499
Size: 871 3460
HindIII A'AGCT_T
Cuts at: 1223 1223
Size: 4331
KasI G'GCGC_C
Cuts at: 120 1761 2009 120
Size: 1641 248 2442
Fragments arranged by size:
2442 1641 248
KpnI G_GTAC'C
Cuts at: 451 1351 451
Size: 900 3431
MluI A'CGCG_T
Cuts at: 3764 3764
Size: 4331
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 356 1087 3002 3186 356
Size: 731 1915 184 1501
Fragments arranged by size:
1915 1501 731 184
MscI TGG'CCA
Cuts at: 2092 2092
Size: 4331
MslI CAynn'nnrTG
Cuts at: 1186 2320 2447 1186
Size: 1134 127 3070
Fragments arranged by size:
3070 1134 127
MspA1I CmG'CkG
Cuts at: 1305 1442 1756 2116 3129 3374 1305
Size: 137 314 360 1013 245 2262
Fragments arranged by size:
2262 1013 360 314 245 137
NaeI GCC'GGC
Cuts at: 2512 2512
Size: 4331
NarI GG'CG_CC
Cuts at: 121 1762 2010 121
Size: 1641 248 2442
Fragments arranged by size:
2442 1641 248
NcoI C'CATG_G
Cuts at: 107 308 698 107
Size: 201 390 3740
Fragments arranged by size:
3740 390 201
NgoMIV G'CCGG_C
Cuts at: 2510 2510
Size: 4331
NspI r_CATG'y
Cuts at: 549 1384 1884 2791 549
Size: 835 500 907 2089
Fragments arranged by size:
2089 907 835 500
PasI CC'CwG_GG
Cuts at: 555 555
Size: 4331
PciI A'CATG_T
Cuts at: 2787 2787
Size: 4331
PflMI CCAn_nnn'nTGG
Cuts at: 391 618 391
Size: 227 4104
PmeI GTTT'AAAC
Cuts at: 1330 1330
Size: 4331
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3470 3502 3470
Size: 32 4299
PpuMI rG'GwC_Cy
Cuts at: 559 559
Size: 4331
PspOMI G'GGCC_C
Cuts at: 261 480 531 660 726 1063 261
Size: 219 51 129 66 337 3529
Fragments arranged by size:
3529 337 219 129 66 51
PstI C_TGCA'G
Cuts at: 1378 2063 1378
Size: 685 3646
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4331
PvuII CAG'CTG
Cuts at: 1305 1756 2116 1305
Size: 451 360 3520
Fragments arranged by size:
3520 451 360
RsrII CG'GwC_CG
Cuts at: 2526 2526
Size: 4331
SacI G_AGCT'C
Cuts at: 1345 1345
Size: 4331
SalI G'TCGA_C
Cuts at: 497 1368 497
Size: 871 3460
SapI GCTCTTCn'nnn_
Cuts at: 2354 2564 2354
Size: 210 4121
SbfI CC_TGCA'GG
Cuts at: 1378 1378
Size: 4331
ScaI AGT'ACT
Cuts at: 3848 3848
Size: 4331
SexAI A'CCwGG_T
Cuts at: 320 795 320
Size: 475 3856
SfcI C'TryA_G
Cuts at: 33 459 1374 2059 3052 3243 33
Size: 426 915 685 993 191 1121
Fragments arranged by size:
1121 993 915 685 426 191
SfoI GGC'GCC
Cuts at: 122 1763 2011 122
Size: 1641 248 2442
Fragments arranged by size:
2442 1641 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4331
SmaI CCC'GGG
Cuts at: 1353 1353
Size: 4331
SmlI C'TyrA_G
Cuts at: 1255 1486 2401 2893 3155 1255
Size: 231 915 492 262 2431
Fragments arranged by size:
2431 915 492 262 231
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4331
SphI G_CATG'C
Cuts at: 1384 1884 1384
Size: 500 3831
StuI AGG'CCT
Cuts at: 1150 1150
Size: 4331
StyI C'CwwG_G
Cuts at: 107 308 390 698 1262 1465 1819 107
Size: 201 82 308 564 203 354 2619
Fragments arranged by size:
2619 564 354 308 203 201 82
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 708 1914 2047 2506 2715 708
Size: 1206 133 459 209 2324
Fragments arranged by size:
2324 1206 459 209 133
TatI w'GTAC_w
Cuts at: 3846 3846
Size: 4331
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 497 529 925 957 3848 3880 497
Size: 32 396 32 2891 32 948
Fragments arranged by size:
2891 948 396 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 797 2128 797
Size: 1331 3000
XbaI T'CTAG_A
Cuts at: 1362 1362
Size: 4331
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 720 1826 720
Size: 1106 3225
XmaI C'CCGG_G
Cuts at: 1351 1351
Size: 4331
XmnI GAAnn'nnTTC
Cuts at: 1165 1165
Size: 4331
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI
BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI CspCI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
HindIII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PpiI PpuMI PspOMI PstI PvuI PvuII RsrII
SacI SalI SapI SbfI ScaI SexAI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XcmI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I
BsiWI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I
ClaI DraIII EcoRV FseI FspAI HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PmlI PshAI
PsiI PspXI PsrI SacII SanDI SfiI SgrAI SnaBI
SrfI SspI SwaI XhoI ZraI