Restriction map for
pF1KB7671
(Circular) (Six-base) MAPSORT of: pF1KB7671.seq Check: 3581 from: 1 to: 4127
pF1KB7671 4127 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 621 678 770 621
Size: 57 92 3978
Fragments arranged by size:
3978 92 57
AccI GT'mk_AC
Cuts at: 1165 1165
Size: 4127
Acc65I G'GTAC_C
Cuts at: 1143 1143
Size: 4127
AclI AA'CG_TT
Cuts at: 3967 3967
Size: 4127
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1952 2384 3131 3399 1952
Size: 432 747 268 2680
Fragments arranged by size:
2680 747 432 268
AfeI AGC'GCT
Cuts at: 451 604 451
Size: 153 3974
AflIII A'CryG_T
Cuts at: 653 1479 2583 3560 653
Size: 826 1104 977 1220
Fragments arranged by size:
1220 1104 977 826
AgeI A'CCGG_T
Cuts at: 2515 2515
Size: 4127
AleI CACnn'nnGTG
Cuts at: 922 922
Size: 4127
AlwNI CAG_nnn'CTG
Cuts at: 465 591 762 777 969 1432 2999 465
Size: 126 171 15 192 463 1567 1593
Fragments arranged by size:
1593 1567 463 192 171 126 15
ApaI G_GGCC'C
Cuts at: 871 871
Size: 4127
ApaLI G'TGCA_C
Cuts at: 2897 2897
Size: 4127
ApoI r'AATT_y
Cuts at: 1131 3480 3736 1131
Size: 2349 256 1522
Fragments arranged by size:
2349 1522 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4127
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
AvaI C'yCGr_G
Cuts at: 220 1147 220
Size: 927 3200
BamHI G'GATC_C
Cuts at: 1152 1152
Size: 4127
BanI G'GyrC_C
Cuts at: 123 138 949 1143 1557 1805 1840 123
Size: 15 811 194 414 248 35 2410
Fragments arranged by size:
2410 811 414 248 194 35 15
BanII G_rGCy'C
Cuts at: 191 871 1141 2171 191
Size: 680 270 1030 2147
Fragments arranged by size:
2147 1030 680 270
BbeI G_GCGC'C
Cuts at: 1561 1809 1561
Size: 248 3879
BbsI GAAGACnn'nnnn_
Cuts at: 421 421
Size: 4127
BciVI GTATCCnnnnn_n'
Cuts at: 2025 2786 4127 2025
Size: 761 1341 2025
Fragments arranged by size:
2025 1341 761
BclI T'GATC_A
Cuts at: 1647 1647
Size: 4127
BglII A'GATC_T
Cuts at: 1642 3679 1642
Size: 2037 2090
BlpI GC'TnA_GC
Cuts at: 355 466 1239 355
Size: 111 773 3243
Fragments arranged by size:
3243 773 111
Bme1580I G_kGCm'C
Cuts at: 128 143 871 952 1752 1845 2901 128
Size: 15 728 81 800 93 1056 1354
Fragments arranged by size:
1354 1056 800 728 93 81 15
BmrI ACTGGGnnnn_n'
Cuts at: 1339 1420 1755 3899 1339
Size: 81 335 2144 1567
Fragments arranged by size:
2144 1567 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 370 2494 370
Size: 2124 2003
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 424 643 664 763 991 358
Size: 66 219 21 99 228 3494
Fragments arranged by size:
3494 228 219 99 66 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 304 430 655 1303 2182 2674 2972 304
Size: 126 225 648 879 492 298 1459
Fragments arranged by size:
1459 879 648 492 298 225 126
BsaI GGTCTCn'nnnn_
Cuts at: 3831 3831
Size: 4127
BsaAI yAC'GTr
Cuts at: 1480 1480
Size: 4127
BsaBI GATnn'nnATC
Cuts at: 6 1641 3678 6
Size: 1635 2037 455
Fragments arranged by size:
2037 1635 455
BsaHI Gr'CG_yC
Cuts at: 1073 1558 1806 2508 4004 1073
Size: 485 248 702 1496 1196
Fragments arranged by size:
1496 1196 702 485 248
BsaWI w'CCGG_w
Cuts at: 1316 1837 2515 2789 2936 3347 1316
Size: 521 678 274 147 411 2096
Fragments arranged by size:
2096 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 382 412 1030 1060 1084 1114 382
Size: 30 618 30 24 30 3395
Fragments arranged by size:
3395 618 30 30 30 24
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 640 821 824 864 988 991 1108 640
Size: 181 3 40 124 3 117 3659
Fragments arranged by size:
3659 181 124 117 40 3 3
BseYI C'CCAG_C
Cuts at: 770 1552 2310 2887 3383 770
Size: 782 758 577 496 1514
Fragments arranged by size:
1514 782 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 712 946 712
Size: 234 3893
BsiEI CG_ry'CG
Cuts at: 104 1669 1715 2923 104
Size: 1565 46 1208 1308
Fragments arranged by size:
1565 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1141 1919 2109 2901 1141
Size: 778 190 792 2367
Fragments arranged by size:
2367 792 778 190
BsmBI CGTCTCn'nnnn_
Cuts at: 276 276
Size: 4127
Bsp1286I G_dGCh'C
Cuts at: 128 143 191 871 952 1141 1752 1845 1919
Size: 15 48 680 81 189 611 93 74
Cuts at: 1919 2109 2171 2901 128
Size: 190 62 730 1354
Fragments arranged by size:
1354 730 680 611 190 189 93 81
74 62 48 15
BspEI T'CCGG_A
Cuts at: 3347 3347
Size: 4127
BspHI T'CATG_A
Cuts at: 3303 3303
Size: 4127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 566 621 678 770 1177 1391 1693 2074 566
Size: 55 57 92 407 214 302 381 2619
Fragments arranged by size:
2619 407 381 302 214 92 57 55
BsrBI CCG'CTC
Cuts at: 50 243 279 470 747 2419 2473 3957 4122
Size: 193 36 191 277 1672 54 1484 165
Cuts at: 4122 50
Size: 55
Fragments arranged by size:
1672 1484 277 193 191 165 55 54
36
BsrDI GCAATG_nn'
Cuts at: 2039 2039
Size: 4127
BsrFI r'CCGG_y
Cuts at: 2125 2306 2515 2125
Size: 181 209 3737
Fragments arranged by size:
3737 209 181
BssSI C'ACGA_G
Cuts at: 2398 2756 2398
Size: 358 3769
BstAPI GCAn_nnn'nTGC
Cuts at: 690 762 1504 690
Size: 72 742 3313
Fragments arranged by size:
3313 742 72
BstBI TT'CG_AA
Cuts at: 2488 2488
Size: 4127
BstYI r'GATC_y
Cuts at: 1 1152 1619 1642 1977 2223 3235 3321 3333
Size: 1151 467 23 335 246 1012 86 12
Cuts at: 3333 3679 1
Size: 346 449
Fragments arranged by size:
1151 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 806 863 1076 107
Size: 699 57 213 3158
Fragments arranged by size:
3158 699 213 57
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2077 2258 2421 2077
Size: 181 163 3783
Fragments arranged by size:
3783 181 163
BtsI GCAGTG_nn'
Cuts at: 1370 1370
Size: 4127
DraI TTT'AAA
Cuts at: 1126 1126
Size: 4127
DrdI GACnn_nn'nnGTC
Cuts at: 1833 2691 1833
Size: 858 3269
EaeI y'GGCC_r
Cuts at: 1712 1886 2277 2304 3649 1712
Size: 174 391 27 1345 2190
Fragments arranged by size:
2190 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1712 1712
Size: 4127
EarI CTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 261 565 2335 2645 2791 3937 261
Size: 304 1770 310 146 1146 451
Fragments arranged by size:
1770 1146 451 310 304 146
EcoICRI GAG'CTC
Cuts at: 1139 1139
Size: 4127
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 358 424 643 664 763 991 1952 2384 3131
Size: 66 219 21 99 228 961 432 747
Cuts at: 3131 3399 358
Size: 268 1086
Fragments arranged by size:
1086 961 747 432 268 228 219 99
66 21
EcoNI CCTnn'n_nnAGG
Cuts at: 406 406
Size: 4127
EcoO109I rG'GnC_Cy
Cuts at: 555 868 1026 1266 555
Size: 313 158 240 3416
Fragments arranged by size:
3416 313 240 158
EcoRI G'AATT_C
Cuts at: 1131 1131
Size: 4127
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2146 2146
Size: 4127
FspI TGC'GCA
Cuts at: 613 683 1908 613
Size: 70 1225 2832
Fragments arranged by size:
2832 1225 70
FspAI rTGC'GCAy
Cuts at: 613 613
Size: 4127
HaeII r_GCGC'y
Cuts at: 453 606 1561 1809 2831 3810 453
Size: 153 955 248 1022 979 770
Fragments arranged by size:
1022 979 955 770 248 153
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 980 1012 1030 1062 1084 1116 1969 1999 2001
Size: 32 18 32 22 32 853 30 2
Cuts at: 2001 2031 980
Size: 30 3076
Fragments arranged by size:
3076 853 32 32 32 30 30 22
18 2
HincII GTy'rAC
Cuts at: 1166 1166
Size: 4127
KasI G'GCGC_C
Cuts at: 1557 1805 1557
Size: 248 3879
KpnI G_GTAC'C
Cuts at: 1147 1147
Size: 4127
MluI A'CGCG_T
Cuts at: 3560 3560
Size: 4127
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1073 2798 2982 1073
Size: 1725 184 2218
Fragments arranged by size:
2218 1725 184
MscI TGG'CCA
Cuts at: 1888 1888
Size: 4127
MslI CAynn'nnrTG
Cuts at: 851 922 2116 2243 851
Size: 71 1194 127 2735
Fragments arranged by size:
2735 1194 127 71
MspA1I CmG'CkG
Cuts at: 231 591 696 984 1016 1238 1552 1912 2925
Size: 360 105 288 32 222 314 360 1013
Cuts at: 2925 3170 231
Size: 245 1188
Fragments arranged by size:
1188 1013 360 360 314 288 245 222
105 32
NaeI GCC'GGC
Cuts at: 2308 2308
Size: 4127
NarI GG'CG_CC
Cuts at: 1558 1806 1558
Size: 248 3879
NcoI C'CATG_G
Cuts at: 107 863 1076 107
Size: 756 213 3158
Fragments arranged by size:
3158 756 213
NgoMIV G'CCGG_C
Cuts at: 2306 2306
Size: 4127
NspI r_CATG'y
Cuts at: 291 657 1180 1680 2587 291
Size: 366 523 500 907 1831
Fragments arranged by size:
1831 907 523 500 366
PciI A'CATG_T
Cuts at: 653 2583 653
Size: 1930 2197
PfoI T'CCnGG_A
Cuts at: 458 458
Size: 4127
PmeI GTTT'AAAC
Cuts at: 1126 1126
Size: 4127
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3266 3298 3266
Size: 32 4095
PpuMI rG'GwC_Cy
Cuts at: 555 555
Size: 4127
PspOMI G'GGCC_C
Cuts at: 867 867
Size: 4127
PstI C_TGCA'G
Cuts at: 701 1174 1859 701
Size: 473 685 2969
Fragments arranged by size:
2969 685 473
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4127
PvuII CAG'CTG
Cuts at: 591 696 1016 1552 1912 591
Size: 105 320 536 360 2806
Fragments arranged by size:
2806 536 360 320 105
RsrII CG'GwC_CG
Cuts at: 2322 2322
Size: 4127
SacI G_AGCT'C
Cuts at: 1141 1141
Size: 4127
SalI G'TCGA_C
Cuts at: 1164 1164
Size: 4127
SapI GCTCTTCn'nnn_
Cuts at: 2150 2360 2150
Size: 210 3917
SbfI CC_TGCA'GG
Cuts at: 1174 1174
Size: 4127
ScaI AGT'ACT
Cuts at: 3644 3644
Size: 4127
SfcI C'TryA_G
Cuts at: 33 192 697 1170 1855 2848 3039 33
Size: 159 505 473 685 993 191 1121
Fragments arranged by size:
1121 993 685 505 473 191 159
SfoI GGC'GCC
Cuts at: 1559 1807 1559
Size: 248 3879
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4127
SmaI CCC'GGG
Cuts at: 1149 1149
Size: 4127
SmlI C'TyrA_G
Cuts at: 319 445 670 1282 2197 2689 2951 319
Size: 126 225 612 915 492 262 1495
Fragments arranged by size:
1495 915 612 492 262 225 126
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4127
SphI G_CATG'C
Cuts at: 291 1180 1680 291
Size: 889 500 2738
Fragments arranged by size:
2738 889 500
StuI AGG'CCT
Cuts at: 1060 1060
Size: 4127
StyI C'CwwG_G
Cuts at: 107 863 1076 1261 1615 107
Size: 756 213 185 354 2619
Fragments arranged by size:
2619 756 354 213 185
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 811 867 967 1710 1843 2302 2511 811
Size: 56 100 743 133 459 209 2427
Fragments arranged by size:
2427 743 459 209 133 100 56
TatI w'GTAC_w
Cuts at: 389 3642 389
Size: 3253 874
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3644 3676 3644
Size: 32 4095
Tth111I GACn'n_nGTC
Cuts at: 1924 1924
Size: 4127
XbaI T'CTAG_A
Cuts at: 1158 1158
Size: 4127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1622 1622
Size: 4127
XmaI C'CCGG_G
Cuts at: 1147 1147
Size: 4127
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AleI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PciI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AlfI AloI AscI AvrII BaeI
BbvCI BcgI BfrBI BglI BmgBI BmtI Bpu10I BsiWI
BsmI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PasI PflMI PmlI
PshAI PsiI PspXI PsrI SacII SanDI SexAI SfiI
SgrAI SnaBI SrfI SspI SwaI XhoI XmnI ZraI