Restriction map for
pF1KB7670
(Circular) (Six-base) MAPSORT of: pF1KB7670.seq Check: 3902 from: 1 to: 4298
pF1KB7670 4298 bp
With 182 enzymes: SgfI *
March 2, 2010 14:27 ..
AccI GT'mk_AC
Cuts at: 1336 1336
Size: 4298
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2123 2555 3302 3570 2123
Size: 432 747 268 2851
Fragments arranged by size:
2851 747 432 268
AflIII A'CryG_T
Cuts at: 1650 2754 3731 1650
Size: 1104 977 2217
Fragments arranged by size:
2217 1104 977
AgeI A'CCGG_T
Cuts at: 2686 2686
Size: 4298
AlwNI CAG_nnn'CTG
Cuts at: 458 597 984 1196 1603 3170 458
Size: 139 387 212 407 1567 1586
Fragments arranged by size:
1586 1567 407 387 212 139
ApaI G_GGCC'C
Cuts at: 224 224
Size: 4298
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 571 1302 3651 3907 571
Size: 731 2349 256 962
Fragments arranged by size:
2349 962 731 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 127 217 1318 127
Size: 90 1101 3107
Fragments arranged by size:
3107 1101 90
BamHI G'GATC_C
Cuts at: 1323 1323
Size: 4298
BanI G'GyrC_C
Cuts at: 947 1314 1728 1976 2011 947
Size: 367 414 248 35 3234
Fragments arranged by size:
3234 414 367 248 35
BanII G_rGCy'C
Cuts at: 224 250 649 1084 1225 1312 2342 224
Size: 26 399 435 141 87 1030 2180
Fragments arranged by size:
2180 1030 435 399 141 87 26
BbeI G_GCGC'C
Cuts at: 1732 1980 1732
Size: 248 4050
BbvCI CC'TCA_GC
Cuts at: 1189 1189
Size: 4298
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 811 1088 811
Size: 277 4021
BglII A'GATC_T
Cuts at: 1813 3850 1813
Size: 2037 2261
BlpI GC'TnA_GC
Cuts at: 634 1410 634
Size: 776 3522
Bme1580I G_kGCm'C
Cuts at: 224 1923 2016 3072 224
Size: 1699 93 1056 1450
Fragments arranged by size:
1699 1450 1056 93
BmgBI CAC'GTC
Cuts at: 1218 1218
Size: 4298
BmrI ACTGGGnnnn_n'
Cuts at: 1510 1591 1926 4070 1510
Size: 81 335 2144 1738
Fragments arranged by size:
2144 1738 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 678 2665 678
Size: 1987 2311
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 523 157
Size: 366 3932
Bpu10I CC'TnA_GC
Cuts at: 1189 1189
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 565 1474 2353 2845 3143 565
Size: 909 879 492 298 1720
Fragments arranged by size:
1720 909 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 831 4002 831
Size: 3171 1127
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 1729 1977 2679 4175 1729
Size: 248 702 1496 1852
Fragments arranged by size:
1852 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1487 2008 2686 2960 3107 3518 1487
Size: 521 678 274 147 411 2267
Fragments arranged by size:
2267 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 496 526 985 1015 1207 1237 1273 1303 496
Size: 30 459 30 192 30 36 30 3491
Fragments arranged by size:
3491 459 192 36 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 214 355 1200 214
Size: 141 845 3312
Fragments arranged by size:
3312 845 141
BseYI C'CCAG_C
Cuts at: 243 977 984 1723 2481 3058 3554 243
Size: 734 7 739 758 577 496 987
Fragments arranged by size:
987 758 739 734 577 496 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 471 471
Size: 4298
BsiEI CG_ry'CG
Cuts at: 104 303 1257 1840 1886 3094 104
Size: 199 954 583 46 1208 1308
Fragments arranged by size:
1308 1208 954 583 199 46
BsiHKAI G_wGCw'C
Cuts at: 206 581 1312 2090 2280 3072 206
Size: 375 731 778 190 792 1432
Fragments arranged by size:
1432 792 778 731 375 190
BsmBI CGTCTCn'nnnn_
Cuts at: 865 865
Size: 4298
Bsp1286I G_dGCh'C
Cuts at: 206 224 250 581 649 1084 1225 1312 1923
Size: 18 26 331 68 435 141 87 611
Cuts at: 1923 2016 2090 2280 2342 3072 206
Size: 93 74 190 62 730 1432
Fragments arranged by size:
1432 730 611 435 331 190 141 93
87 74 68 62 26 18
BspEI T'CCGG_A
Cuts at: 3518 3518
Size: 4298
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 638 1348 1562 1864 2245 638
Size: 710 214 302 381 2691
Fragments arranged by size:
2691 710 381 302 214
BsrBI CCG'CTC
Cuts at: 50 549 2590 2644 4128 4293 50
Size: 499 2041 54 1484 165 55
Fragments arranged by size:
2041 1484 499 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 699 1256 2296 2477 2686 699
Size: 557 1040 181 209 2311
Fragments arranged by size:
2311 1040 557 209 181
BssSI C'ACGA_G
Cuts at: 337 2569 2927 337
Size: 2232 358 1708
Fragments arranged by size:
2232 1708 358
BstAPI GCAn_nnn'nTGC
Cuts at: 210 458 1675 210
Size: 248 1217 2833
Fragments arranged by size:
2833 1217 248
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 180 1011 180
Size: 831 3467
BstYI r'GATC_y
Cuts at: 1 1323 1790 1813 2148 2394 3406 3492 3504
Size: 1322 467 23 335 246 1012 86 12
Cuts at: 3504 3850 1
Size: 346 449
Fragments arranged by size:
1322 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 754 1070 107
Size: 647 316 3335
Fragments arranged by size:
3335 647 316
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 358 1541 358
Size: 1183 3115
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 443 701 1067 1883 2057 2448 2475 3820 443
Size: 258 366 816 174 391 27 1345 921
Fragments arranged by size:
1345 921 816 391 366 258 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 442 664 795 2321 2531 442
Size: 222 131 1526 210 2209
Fragments arranged by size:
2209 1526 222 210 131
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2506 2816 2962 4108 2506
Size: 310 146 1146 2696
Fragments arranged by size:
2696 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 157 523 2123 2555 3302 3570 157
Size: 366 1600 432 747 268 885
Fragments arranged by size:
1600 885 747 432 366 268
EcoO109I rG'GnC_Cy
Cuts at: 124 627 750 996 1113 1437 124
Size: 503 123 246 117 324 2985
Fragments arranged by size:
2985 503 324 246 123 117
EcoRI G'AATT_C
Cuts at: 571 1302 571
Size: 731 3567
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 834 2317 834
Size: 1483 2815
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 502 1732 1980 3002 3981 502
Size: 1230 248 1022 979 819
Fragments arranged by size:
1230 1022 979 819 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 814 846 985 1017 1207 1239 2140 2170 2172
Size: 32 139 32 190 32 901 30 2
Cuts at: 2172 2202 814
Size: 30 2910
Fragments arranged by size:
2910 901 190 139 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 321 1337 321
Size: 1016 3282
KasI G'GCGC_C
Cuts at: 1728 1976 1728
Size: 248 4050
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 161 275 323 683 2969 3153 161
Size: 114 48 360 2286 184 1306
Fragments arranged by size:
2286 1306 360 184 114 48
MscI TGG'CCA
Cuts at: 1069 2059 1069
Size: 990 3308
MslI CAynn'nnrTG
Cuts at: 193 2287 2414 193
Size: 2094 127 2077
Fragments arranged by size:
2094 2077 127
MspA1I CmG'CkG
Cuts at: 288 386 455 594 734 756 868 981 1041
Size: 98 69 139 140 22 112 113 60
Cuts at: 1041 1409 1723 2083 3096 3341 288
Size: 368 314 360 1013 245 1245
Fragments arranged by size:
1245 1013 368 360 314 245 140 139
113 112 98 69 60 22
NaeI GCC'GGC
Cuts at: 701 2479 701
Size: 1778 2520
NarI GG'CG_CC
Cuts at: 1729 1977 1729
Size: 248 4050
NcoI C'CATG_G
Cuts at: 107 1070 107
Size: 963 3335
NgoMIV G'CCGG_C
Cuts at: 699 2477 699
Size: 1778 2520
NspI r_CATG'y
Cuts at: 1351 1851 2758 1351
Size: 500 907 2891
Fragments arranged by size:
2891 907 500
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PflMI CCAn_nnn'nTGG
Cuts at: 984 984
Size: 4298
PfoI T'CCnGG_A
Cuts at: 395 395
Size: 4298
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PpuMI rG'GwC_Cy
Cuts at: 124 627 750 996 124
Size: 503 123 246 3426
Fragments arranged by size:
3426 503 246 123
PspOMI G'GGCC_C
Cuts at: 220 220
Size: 4298
PstI C_TGCA'G
Cuts at: 1232 1345 2030 1232
Size: 113 685 3500
Fragments arranged by size:
3500 685 113
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 386 594 868 1723 2083 386
Size: 208 274 855 360 2601
Fragments arranged by size:
2601 855 360 274 208
RsrII CG'GwC_CG
Cuts at: 2493 2493
Size: 4298
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SacII CC_GC'GG
Cuts at: 757 757
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 442 664 795 2321 2531 442
Size: 222 131 1526 210 2209
Fragments arranged by size:
2209 1526 222 210 131
SbfI CC_TGCA'GG
Cuts at: 1232 1345 1232
Size: 113 4185
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 1228 1341 2026 3019 3210 33
Size: 1195 113 685 993 191 1121
Fragments arranged by size:
1195 1121 993 685 191 113
SfoI GGC'GCC
Cuts at: 1730 1978 1730
Size: 248 4050
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 219 1320 219
Size: 1101 3197
SmlI C'TyrA_G
Cuts at: 580 1453 2368 2860 3122 580
Size: 873 915 492 262 1756
Fragments arranged by size:
1756 915 873 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
StuI AGG'CCT
Cuts at: 814 814
Size: 4298
StyI C'CwwG_G
Cuts at: 107 704 1070 1432 1786 107
Size: 597 366 362 354 2619
Fragments arranged by size:
2619 597 366 362 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1881 2014 2473 2682 1881
Size: 133 459 209 3497
Fragments arranged by size:
3497 459 209 133
TatI w'GTAC_w
Cuts at: 410 3813 410
Size: 3403 895
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1793 1793
Size: 4298
XmaI C'CCGG_G
Cuts at: 217 1318 217
Size: 1101 3197
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbvCI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PciI PflMI PfoI PmeI
PpiI PpuMI PspOMI PstI PvuI PvuII RsrII SacI
SacII SalI SapI SbfI ScaI SfcI SfoI SgfI
SmaI SmlI SpeI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
AscI AvrII BaeI BbsI BcgI BfrBI BmtI BsiWI
BsmI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI NsiI PacI PasI PmlI
PshAI PsiI PspXI PsrI SanDI SexAI SfiI SgrAI
SnaBI SrfI SspI SwaI XhoI XmnI ZraI