Restriction map for
pF1KB7668
(Circular) (Six-base) MAPSORT of: pF1KB7668.seq Check: 958 from: 1 to: 4319
pF1KB7668 4319 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 388 388
Size: 4319
AccI GT'mk_AC
Cuts at: 1357 1357
Size: 4319
Acc65I G'GTAC_C
Cuts at: 1335 1335
Size: 4319
AclI AA'CG_TT
Cuts at: 4159 4159
Size: 4319
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2144 2576 3323 3591 2144
Size: 432 747 268 2872
Fragments arranged by size:
2872 747 432 268
AfeI AGC'GCT
Cuts at: 1082 1082
Size: 4319
AflIII A'CryG_T
Cuts at: 1671 2775 3752 1671
Size: 1104 977 2238
Fragments arranged by size:
2238 1104 977
AgeI A'CCGG_T
Cuts at: 2707 2707
Size: 4319
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 787 819 787
Size: 32 4287
AlwNI CAG_nnn'CTG
Cuts at: 1624 3191 1624
Size: 1567 2752
ApaI G_GGCC'C
Cuts at: 1226 1226
Size: 4319
ApaLI G'TGCA_C
Cuts at: 115 3089 115
Size: 2974 1345
ApoI r'AATT_y
Cuts at: 1323 3672 3928 1323
Size: 2349 256 1714
Fragments arranged by size:
2349 1714 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4319
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
AvaI C'yCGr_G
Cuts at: 633 1165 1288 1339 633
Size: 532 123 51 3613
Fragments arranged by size:
3613 532 123 51
AvrII C'CTAG_G
Cuts at: 837 837
Size: 4319
BamHI G'GATC_C
Cuts at: 1344 1344
Size: 4319
BanI G'GyrC_C
Cuts at: 637 841 1249 1335 1749 1997 2032 637
Size: 204 408 86 414 248 35 2924
Fragments arranged by size:
2924 414 408 248 204 86 35
BanII G_rGCy'C
Cuts at: 422 472 1165 1226 1295 1333 2363 422
Size: 50 693 61 69 38 1030 2378
Fragments arranged by size:
2378 1030 693 69 61 50 38
BbeI G_GCGC'C
Cuts at: 845 1753 2001 845
Size: 908 248 3163
Fragments arranged by size:
3163 908 248
BciVI GTATCCnnnnn_n'
Cuts at: 2217 2978 4319 2217
Size: 761 1341 2217
Fragments arranged by size:
2217 1341 761
BclI T'GATC_A
Cuts at: 1839 1839
Size: 4319
BglI GCCn_nnn'nGGC
Cuts at: 1181 1181
Size: 4319
BglII A'GATC_T
Cuts at: 1834 3871 1834
Size: 2037 2282
BlpI GC'TnA_GC
Cuts at: 829 1431 829
Size: 602 3717
Bme1580I G_kGCm'C
Cuts at: 119 640 1186 1226 1944 2037 3093 119
Size: 521 546 40 718 93 1056 1345
Fragments arranged by size:
1345 1056 718 546 521 93 40
BmrI ACTGGGnnnn_n'
Cuts at: 1531 1612 1947 4091 1531
Size: 81 335 2144 1759
Fragments arranged by size:
2144 1759 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 648 2686 648
Size: 2038 2281
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 703 1020 526
Size: 177 317 3825
Fragments arranged by size:
3825 317 177
Bpu10I CC'TnA_GC
Cuts at: 466 466
Size: 4319
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 466 676 1495 2374 2866 3164 466
Size: 210 819 879 492 298 1621
Fragments arranged by size:
1621 879 819 492 298 210
BsaI GGTCTCn'nnnn_
Cuts at: 748 4023 748
Size: 3275 1044
BsaAI yAC'GTr
Cuts at: 1672 1672
Size: 4319
BsaBI GATnn'nnATC
Cuts at: 6 1833 3870 6
Size: 1827 2037 455
Fragments arranged by size:
2037 1827 455
BsaHI Gr'CG_yC
Cuts at: 842 1069 1750 1998 2700 4196 842
Size: 227 681 248 702 1496 965
Fragments arranged by size:
1496 965 702 681 248 227
BsaWI w'CCGG_w
Cuts at: 863 1508 2029 2707 2981 3128 3539 863
Size: 645 521 678 274 147 411 1643
Fragments arranged by size:
1643 678 645 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 430 637 430
Size: 207 4112
BseYI C'CCAG_C
Cuts at: 325 888 1074 1225 1744 2502 3079 3575 325
Size: 563 186 151 519 758 577 496 1069
Fragments arranged by size:
1069 758 577 563 519 496 186 151
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1174 1174
Size: 4319
BsiEI CG_ry'CG
Cuts at: 104 150 897 1031 1861 1907 3115 104
Size: 46 747 134 830 46 1208 1308
Fragments arranged by size:
1308 1208 830 747 134 46 46
BsiHKAI G_wGCw'C
Cuts at: 119 422 472 1295 1333 2111 2301 3093 119
Size: 303 50 823 38 778 190 792 1345
Fragments arranged by size:
1345 823 792 778 303 190 50 38
BsmBI CGTCTCn'nnnn_
Cuts at: 565 595 679 565
Size: 30 84 4205
Fragments arranged by size:
4205 84 30
Bsp1286I G_dGCh'C
Cuts at: 119 422 472 640 1165 1186 1226 1295 1333
Size: 303 50 168 525 21 40 69 38
Cuts at: 1333 1944 2037 2111 2301 2363 3093 119
Size: 611 93 74 190 62 730 1345
Fragments arranged by size:
1345 730 611 525 303 190 168 93
74 69 62 50 40 38 21
BspEI T'CCGG_A
Cuts at: 863 3539 863
Size: 2676 1643
BspHI T'CATG_A
Cuts at: 3495 3495
Size: 4319
BspMI ACCTGCnnnn'nnnn_
Cuts at: 388 1369 1583 1885 2266 388
Size: 981 214 302 381 2441
Fragments arranged by size:
2441 981 381 302 214
BsrBI CCG'CTC
Cuts at: 50 135 1095 1247 2611 2665 4149 4314 50
Size: 85 960 152 1364 54 1484 165 55
Fragments arranged by size:
1484 1364 960 165 152 85 55 54
BsrDI GCAATG_nn'
Cuts at: 2231 2231
Size: 4319
BsrFI r'CCGG_y
Cuts at: 124 301 409 757 1099 2317 2498 2707 124
Size: 177 108 348 342 1218 181 209 1736
Fragments arranged by size:
1736 1218 348 342 209 181 177 108
BssHII G'CGCG_C
Cuts at: 308 308
Size: 4319
BssSI C'ACGA_G
Cuts at: 2590 2948 2590
Size: 358 3961
BstAPI GCAn_nnn'nTGC
Cuts at: 296 1197 1696 296
Size: 901 499 2919
Fragments arranged by size:
2919 901 499
BstBI TT'CG_AA
Cuts at: 2680 2680
Size: 4319
BstXI CCAn_nnnn'nTGG
Cuts at: 333 681 1044 333
Size: 348 363 3608
Fragments arranged by size:
3608 363 348
BstYI r'GATC_y
Cuts at: 1 1344 1811 1834 2169 2415 3427 3513 3525
Size: 1343 467 23 335 246 1012 86 12
Cuts at: 3525 3871 1
Size: 346 449
Fragments arranged by size:
1343 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 871 1112 871
Size: 241 4078
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2269 2450 2613 2269
Size: 181 163 3975
Fragments arranged by size:
3975 181 163
BtsI GCAGTG_nn'
Cuts at: 117 550 1562 117
Size: 433 1012 2874
Fragments arranged by size:
2874 1012 433
DraI TTT'AAA
Cuts at: 1318 1318
Size: 4319
DrdI GACnn_nn'nnGTC
Cuts at: 2025 2883 2025
Size: 858 3461
EaeI y'GGCC_r
Cuts at: 605 894 1028 1904 2078 2469 2496 3841 605
Size: 289 134 876 174 391 27 1345 1083
Fragments arranged by size:
1345 1083 876 391 289 174 134 27
EagI C'GGCC_G
Cuts at: 894 1028 1904 894
Size: 134 876 3309
Fragments arranged by size:
3309 876 134
EarI CTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2527 2837 2983 4129 2527
Size: 310 146 1146 2717
Fragments arranged by size:
2717 1146 310 146
EcoICRI GAG'CTC
Cuts at: 420 470 1293 1331 420
Size: 50 823 38 3408
Fragments arranged by size:
3408 823 50 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 526 703 1020 2144 2576 3323 3591 526
Size: 177 317 1124 432 747 268 1254
Fragments arranged by size:
1254 1124 747 432 317 268 177
EcoNI CCTnn'n_nnAGG
Cuts at: 484 484
Size: 4319
EcoO109I rG'GnC_Cy
Cuts at: 1458 1458
Size: 4319
EcoRI G'AATT_C
Cuts at: 1323 1323
Size: 4319
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 717 2338 717
Size: 1621 2698
FspI TGC'GCA
Cuts at: 340 2100 340
Size: 1760 2559
HaeII r_GCGC'y
Cuts at: 285 836 845 1084 1753 2001 3023 4002 285
Size: 551 9 239 669 248 1022 979 602
Fragments arranged by size:
1022 979 669 602 551 248 239 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1126 1158 2161 2191 2193 2223 1126
Size: 32 1003 30 2 30 3222
Fragments arranged by size:
3222 1003 32 30 30 2
HincII GTy'rAC
Cuts at: 1358 1358
Size: 4319
KasI G'GCGC_C
Cuts at: 841 1749 1997 841
Size: 908 248 3163
Fragments arranged by size:
3163 908 248
KpnI G_GTAC'C
Cuts at: 1339 1339
Size: 4319
MluI A'CGCG_T
Cuts at: 3752 3752
Size: 4319
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 572 2990 3174 572
Size: 2418 184 1717
Fragments arranged by size:
2418 1717 184
MscI TGG'CCA
Cuts at: 2080 2080
Size: 4319
MslI CAynn'nnrTG
Cuts at: 112 382 781 1135 2308 2435 112
Size: 270 399 354 1173 127 1996
Fragments arranged by size:
1996 1173 399 354 270 127
MspA1I CmG'CkG
Cuts at: 402 681 744 828 873 888 1074 1114 1217
Size: 279 63 84 45 15 186 40 103
Cuts at: 1217 1256 1265 1430 1744 2104 3117 3362 402
Size: 39 9 165 314 360 1013 245 1359
Fragments arranged by size:
1359 1013 360 314 279 245 186 165
103 84 63 45 40 39 15 9
NaeI GCC'GGC
Cuts at: 303 1101 2500 303
Size: 798 1399 2122
Fragments arranged by size:
2122 1399 798
NarI GG'CG_CC
Cuts at: 842 1750 1998 842
Size: 908 248 3163
Fragments arranged by size:
3163 908 248
NgoMIV G'CCGG_C
Cuts at: 301 1099 2498 301
Size: 798 1399 2122
Fragments arranged by size:
2122 1399 798
NotI GC'GGCC_GC
Cuts at: 894 894
Size: 4319
NspI r_CATG'y
Cuts at: 771 882 1372 1872 2779 771
Size: 111 490 500 907 2311
Fragments arranged by size:
2311 907 500 490 111
PciI A'CATG_T
Cuts at: 2775 2775
Size: 4319
PfoI T'CCnGG_A
Cuts at: 542 542
Size: 4319
PmeI GTTT'AAAC
Cuts at: 1318 1318
Size: 4319
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 427 459 1079 1111 3458 3490 427
Size: 32 620 32 2347 32 1256
Fragments arranged by size:
2347 1256 620 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1272 1272
Size: 4319
PspOMI G'GGCC_C
Cuts at: 1222 1222
Size: 4319
PstI C_TGCA'G
Cuts at: 557 1366 2051 557
Size: 809 685 2825
Fragments arranged by size:
2825 809 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4319
PvuII CAG'CTG
Cuts at: 402 681 744 828 1744 2104 402
Size: 279 63 84 916 360 2617
Fragments arranged by size:
2617 916 360 279 84 63
RsrII CG'GwC_CG
Cuts at: 519 2514 519
Size: 1995 2324
SacI G_AGCT'C
Cuts at: 422 472 1295 1333 422
Size: 50 823 38 3408
Fragments arranged by size:
3408 823 50 38
SacII CC_GC'GG
Cuts at: 874 1115 874
Size: 241 4078
SalI G'TCGA_C
Cuts at: 1356 1356
Size: 4319
SapI GCTCTTCn'nnn_
Cuts at: 2342 2552 2342
Size: 210 4109
SbfI CC_TGCA'GG
Cuts at: 1366 1366
Size: 4319
ScaI AGT'ACT
Cuts at: 3836 3836
Size: 4319
SexAI A'CCwGG_T
Cuts at: 332 332
Size: 4319
SfcI C'TryA_G
Cuts at: 33 553 1260 1362 2047 3040 3231 33
Size: 520 707 102 685 993 191 1121
Fragments arranged by size:
1121 993 707 685 520 191 102
SfoI GGC'GCC
Cuts at: 843 1751 1999 843
Size: 908 248 3163
Fragments arranged by size:
3163 908 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4319
SgrAI Cr'CCGG_yG
Cuts at: 409 409
Size: 4319
SmaI CCC'GGG
Cuts at: 1341 1341
Size: 4319
SmlI C'TyrA_G
Cuts at: 481 691 1474 2389 2881 3143 481
Size: 210 783 915 492 262 1657
Fragments arranged by size:
1657 915 783 492 262 210
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4319
SphI G_CATG'C
Cuts at: 882 1372 1872 882
Size: 490 500 3329
Fragments arranged by size:
3329 500 490
StuI AGG'CCT
Cuts at: 1175 1175
Size: 4319
StyI C'CwwG_G
Cuts at: 837 954 1453 1807 837
Size: 117 499 354 3349
Fragments arranged by size:
3349 499 354 117
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1902 2035 2494 2703 1902
Size: 133 459 209 3518
Fragments arranged by size:
3518 459 209 133
TatI w'GTAC_w
Cuts at: 3834 3834
Size: 4319
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 163 195 3836 3868 163
Size: 32 3641 32 614
Fragments arranged by size:
3641 614 32 32
Tth111I GACn'n_nGTC
Cuts at: 588 2116 588
Size: 1528 2791
XbaI T'CTAG_A
Cuts at: 1350 1350
Size: 4319
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1814 1814
Size: 4319
XmaI C'CCGG_G
Cuts at: 1339 1339
Size: 4319
XmnI GAAnn'nnTTC
Cuts at: 235 442 235
Size: 207 4112
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
AvrII BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NotI NspI PciI
PfoI PmeI PpiI PshAI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SapI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AscI BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI BsaXI BsiWI BsmI
BsrGI BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NcoI NdeI NheI
NruI NsiI PacI PasI PflMI PmlI PpuMI PsiI
PspXI PsrI SanDI SfiI SnaBI SrfI SspI SwaI
XhoI ZraI