Restriction map for
pF1KB7667
(Circular) (Six-base) MAPSORT of: pF1KB7667.seq Check: 2244 from: 1 to: 4124
pF1KB7667 4124 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 912 912
Size: 4124
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 1140 1140
Size: 4124
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 1949 2381 3128 3396 669
Size: 1280 432 747 268 1397
Fragments arranged by size:
1397 1280 747 432 268
AfeI AGC'GCT
Cuts at: 394 394
Size: 4124
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 459 503 2512 459
Size: 44 2009 2071
Fragments arranged by size:
2071 2009 44
AhdI GACnn_n'nnGTC
Cuts at: 418 418
Size: 4124
AleI CACnn'nnGTG
Cuts at: 859 859
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 622 1128 3477 3733 622
Size: 506 2349 256 1013
Fragments arranged by size:
2349 1013 506 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 1144 1144
Size: 4124
BamHI G'GATC_C
Cuts at: 1149 1149
Size: 4124
BanI G'GyrC_C
Cuts at: 174 1140 1554 1802 1837 174
Size: 966 414 248 35 2461
Fragments arranged by size:
2461 966 414 248 35
BanII G_rGCy'C
Cuts at: 259 1138 2168 259
Size: 879 1030 2215
Fragments arranged by size:
2215 1030 879
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 675 1058 675
Size: 383 3741
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4090
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 515 1644 515
Size: 1129 2995
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 716 1336 1417 1752 3896 716
Size: 620 81 335 2144 944
Fragments arranged by size:
2144 944 620 335 81
BmtI G_CTAG'C
Cuts at: 1033 1033
Size: 4124
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2491 2491
Size: 4124
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 726 842 726
Size: 116 4008
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3828 3828
Size: 4124
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 1555 1803 2505 4001 1555
Size: 248 702 1496 1678
Fragments arranged by size:
1678 1496 702 248
BsaWI w'CCGG_w
Cuts at: 459 503 1313 1834 2512 2786 2933 3344 459
Size: 44 810 521 678 274 147 411 1239
Fragments arranged by size:
1239 810 678 521 411 274 147 44
BseYI C'CCAG_C
Cuts at: 258 1549 2307 2884 3380 258
Size: 1291 758 577 496 1002
Fragments arranged by size:
1291 1002 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 837 837
Size: 4124
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 185 206 1138 1916 2106 2898 185
Size: 21 932 778 190 792 1411
Fragments arranged by size:
1411 932 792 778 190 21
Bsp1286I G_dGCh'C
Cuts at: 185 206 259 1138 1749 1842 1916 2106 2168
Size: 21 53 879 611 93 74 190 62
Cuts at: 2168 2898 185
Size: 730 1411
Fragments arranged by size:
1411 879 730 611 190 93 74 62
53 21
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 3300 3300
Size: 4124
BspMI ACCTGCnnnn'nnnn_
Cuts at: 912 1174 1388 1690 2071 912
Size: 262 214 302 381 2965
Fragments arranged by size:
2965 381 302 262 214
BsrBI CCG'CTC
Cuts at: 50 2416 2470 3954 4119 50
Size: 2366 54 1484 165 55
Fragments arranged by size:
2366 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 668 2036 668
Size: 1368 2756
BsrFI r'CCGG_y
Cuts at: 459 503 2122 2303 2512 459
Size: 44 1619 181 209 2071
Fragments arranged by size:
2071 1619 209 181 44
BsrGI T'GTAC_A
Cuts at: 665 665
Size: 4124
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstEII G'GTnAC_C
Cuts at: 802 802
Size: 4124
BstYI r'GATC_y
Cuts at: 1 1149 1616 1639 1974 2220 3232 3318 3330
Size: 1148 467 23 335 246 1012 86 12
Cuts at: 3330 3676 1
Size: 346 449
Fragments arranged by size:
1148 1012 467 449 346 335 246 86
23 12
Bsu36I CC'TnA_GG
Cuts at: 416 416
Size: 4124
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 537 579 1024 1367 537
Size: 42 445 343 3294
Fragments arranged by size:
3294 445 343 42
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 446 1709 1883 2274 2301 3646 446
Size: 1263 174 391 27 1345 924
Fragments arranged by size:
1345 1263 924 391 174 27
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1136 1136
Size: 4124
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 669 726 842 1949 2381 3128 3396 669
Size: 57 116 1107 432 747 268 1397
Fragments arranged by size:
1397 1107 747 432 268 116 57
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 1128 1128
Size: 4124
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 351 387 2143 351
Size: 36 1756 2332
Fragments arranged by size:
2332 1756 36
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 396 1558 1806 2828 3807 396
Size: 1162 248 1022 979 713
Fragments arranged by size:
1162 1022 979 713 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 404 436 970 1002 1966 1996 1998 2028 404
Size: 32 534 32 964 30 2 30 2500
Fragments arranged by size:
2500 964 534 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1163 1163
Size: 4124
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 1144 1144
Size: 4124
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 638 2795 2979 638
Size: 2157 184 1783
Fragments arranged by size:
2157 1783 184
MscI TGG'CCA
Cuts at: 448 1885 448
Size: 1437 2687
MslI CAynn'nnrTG
Cuts at: 301 859 2113 2240 301
Size: 558 1254 127 2185
Fragments arranged by size:
2185 1254 558 127
MspA1I CmG'CkG
Cuts at: 530 1235 1549 1909 2922 3167 530
Size: 705 314 360 1013 245 1487
Fragments arranged by size:
1487 1013 705 360 314 245
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NheI G'CTAG_C
Cuts at: 1029 1029
Size: 4124
NspI r_CATG'y
Cuts at: 774 1177 1677 2584 774
Size: 403 500 907 2314
Fragments arranged by size:
2314 907 500 403
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3263 3295 3263
Size: 32 4092
PshAI GACnn'nnGTC
Cuts at: 506 506
Size: 4124
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 1138 1138
Size: 4124
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 3641 3641
Size: 4124
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
StuI AGG'CCT
Cuts at: 847 847
Size: 4124
StyI C'CwwG_G
Cuts at: 586 897 1258 1612 586
Size: 311 361 354 3098
Fragments arranged by size:
3098 361 354 311
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 162 1707 1840 2299 2508 162
Size: 1545 133 459 209 1778
Fragments arranged by size:
1778 1545 459 209 133
TatI w'GTAC_w
Cuts at: 665 3639 665
Size: 2974 1150
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3641 3673 3641
Size: 32 4092
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 925 1619 925
Size: 694 3430
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
XmnI GAAnn'nnTTC
Cuts at: 943 943
Size: 4124
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AlwNI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BcgI BciVI BclI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NspI PciI PmeI PpiI PshAI
PstI PvuI PvuII RsrII SacI SalI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AlfI AloI ApaI AscI AvrII BaeI
BbvCI BfrBI BglI BmgBI Bpu10I BsaXI BseRI BsiWI
BsmI BsmBI BssHII BstXI BstZ17I BtgI ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI NsiI PacI PasI PflMI
PfoI PmlI PpuMI PsiI PspOMI PspXI PsrI SacII
SanDI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI ZraI