Restriction map for
pF1KB7666
(Circular) (Six-base) MAPSORT of: pF1KB7666.seq Check: 1687 from: 1 to: 4226
pF1KB7666 4226 bp
With 182 enzymes: SgfI *
January 9, 2015 15:32 ..
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 1242 1242
Size: 4226
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 2051 2483 3230 3498 2051
Size: 432 747 268 2779
Fragments arranged by size:
2779 747 432 268
AflIII A'CryG_T
Cuts at: 482 1578 2682 3659 482
Size: 1096 1104 977 1049
Fragments arranged by size:
1104 1096 1049 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AhdI GACnn_n'nnGTC
Cuts at: 267 267
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 679 1531 3098 679
Size: 852 1567 1807
Fragments arranged by size:
1807 1567 852
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 1230 3579 3835 1230
Size: 2349 256 1621
Fragments arranged by size:
2349 1621 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 432 513 1246 432
Size: 81 733 3412
Fragments arranged by size:
3412 733 81
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 1242 1656 1904 1939 1242
Size: 414 248 35 3529
Fragments arranged by size:
3529 414 248 35
BanII G_rGCy'C
Cuts at: 353 1240 2270 353
Size: 887 1030 2309
Fragments arranged by size:
2309 1030 887
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BbsI GAAGACnn'nnnn_
Cuts at: 263 263
Size: 4226
BbvCI CC'TCA_GC
Cuts at: 875 875
Size: 4226
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4192
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglII A'GATC_T
Cuts at: 215 1741 3778 215
Size: 1526 2037 663
Fragments arranged by size:
2037 1526 663
BlpI GC'TnA_GC
Cuts at: 1338 1338
Size: 4226
Bme1580I G_kGCm'C
Cuts at: 536 1851 1944 3000 536
Size: 1315 93 1056 1762
Fragments arranged by size:
1762 1315 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1438 1519 1854 3998 1438
Size: 81 335 2144 1666
Fragments arranged by size:
2144 1666 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 656 590
Size: 66 4160
Bpu10I CC'TnA_GC
Cuts at: 875 875
Size: 4226
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1402 2281 2773 3071 1402
Size: 879 492 298 2557
Fragments arranged by size:
2557 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 867 1579 867
Size: 712 3514
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1657 1905 2607 4103 1657
Size: 248 702 1496 1780
Fragments arranged by size:
1780 1496 702 248
BsaWI w'CCGG_w
Cuts at: 121 1415 1936 2614 2888 3035 3446 121
Size: 1294 521 678 274 147 411 901
Fragments arranged by size:
1294 901 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 140 140
Size: 4226
BseYI C'CCAG_C
Cuts at: 1651 2409 2986 3482 1651
Size: 758 577 496 2395
Fragments arranged by size:
2395 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 562 1022 562
Size: 460 3766
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 599 1240 2018 2208 3000 599
Size: 641 778 190 792 1825
Fragments arranged by size:
1825 792 778 641 190
BsmI GAATG_Cn'
Cuts at: 939 939
Size: 4226
Bsp1286I G_dGCh'C
Cuts at: 353 536 599 1240 1851 1944 2018 2208 2270
Size: 183 63 641 611 93 74 190 62
Cuts at: 2270 3000 353
Size: 730 1579
Fragments arranged by size:
1579 730 641 611 190 183 93 74
63 62
BspEI T'CCGG_A
Cuts at: 121 3446 121
Size: 3325 901
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1276 1490 1792 2173 1276
Size: 214 302 381 3329
Fragments arranged by size:
3329 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 2224 2405 2614 2224
Size: 181 209 3836
Fragments arranged by size:
3836 209 181
BssSI C'ACGA_G
Cuts at: 316 2497 2855 316
Size: 2181 358 1687
Fragments arranged by size:
2181 1687 358
BstAPI GCAn_nnn'nTGC
Cuts at: 1603 1603
Size: 4226
BstBI TT'CG_AA
Cuts at: 2587 2587
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 790 1021 790
Size: 231 3995
BstYI r'GATC_y
Cuts at: 1 215 493 1251 1718 1741 2076 2322 3334
Size: 214 278 758 467 23 335 246 1012
Cuts at: 3334 3420 3432 3778 1
Size: 86 12 346 449
Fragments arranged by size:
1012 758 467 449 346 335 278 246
214 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 821 821
Size: 4226
BtgI C'CryG_G
Cuts at: 164 536 164
Size: 372 3854
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DraIII CAC_nnn'GTG
Cuts at: 321 321
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 302 323 1811 1985 2376 2403 3748 302
Size: 21 1488 174 391 27 1345 780
Fragments arranged by size:
1488 1345 780 391 174 27 21
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 236 1046 1147 1153 2249 2459 236
Size: 810 101 6 1096 210 2003
Fragments arranged by size:
2003 1096 810 210 101 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 590 656 2051 2483 3230 3498 590
Size: 66 1395 432 747 268 1318
Fragments arranged by size:
1395 1318 747 432 268 66
EcoO109I rG'GnC_Cy
Cuts at: 147 421 1365 147
Size: 274 944 3008
Fragments arranged by size:
3008 944 274
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
EcoRV GAT'ATC
Cuts at: 684 684
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 291 2245 291
Size: 1954 2272
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 1660 1908 2930 3909 1660
Size: 248 1022 979 1977
Fragments arranged by size:
1977 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 219 251 253 285 628 660 753 785 956
Size: 32 2 32 343 32 93 32 171
Cuts at: 956 988 2068 2098 2100 2130 219
Size: 32 1080 30 2 30 2315
Fragments arranged by size:
2315 1080 343 171 93 32 32 32
32 32 30 30 2 2
HincII GTy'rAC
Cuts at: 642 1265 642
Size: 623 3603
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 1246 1246
Size: 4226
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 699 2897 3081 699
Size: 2198 184 1844
Fragments arranged by size:
2198 1844 184
MscI TGG'CCA
Cuts at: 325 1987 325
Size: 1662 2564
MslI CAynn'nnrTG
Cuts at: 380 398 454 2215 2342 380
Size: 18 56 1761 127 2264
Fragments arranged by size:
2264 1761 127 56 18
MspA1I CmG'CkG
Cuts at: 1210 1337 1651 2011 3024 3269 1210
Size: 127 314 360 1013 245 2167
Fragments arranged by size:
2167 1013 360 314 245 127
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NdeI CA'TA_TG
Cuts at: 758 1100 758
Size: 342 3884
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 486 660 1039 1279 1779 2686 486
Size: 174 379 240 500 907 2026
Fragments arranged by size:
2026 907 500 379 240 174
PciI A'CATG_T
Cuts at: 482 2682 482
Size: 2200 2026
PflMI CCAn_nnn'nTGG
Cuts at: 739 739
Size: 4226
PfoI T'CCnGG_A
Cuts at: 606 606
Size: 4226
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PmlI CAC'GTG
Cuts at: 867 867
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PpuMI rG'GwC_Cy
Cuts at: 147 421 147
Size: 274 3952
PspXI vC'TCGA_Gb
Cuts at: 432 432
Size: 4226
PstI C_TGCA'G
Cuts at: 1273 1958 1273
Size: 685 3541
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 1210 1651 2011 1210
Size: 441 360 3425
Fragments arranged by size:
3425 441 360
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 147 421 147
Size: 274 3952
SapI GCTCTTCn'nnn_
Cuts at: 1046 2249 2459 1046
Size: 1203 210 2813
Fragments arranged by size:
2813 1203 210
SbfI CC_TGCA'GG
Cuts at: 1273 1273
Size: 4226
ScaI AGT'ACT
Cuts at: 3743 3743
Size: 4226
SfcI C'TryA_G
Cuts at: 33 1171 1269 1954 2947 3138 33
Size: 1138 98 685 993 191 1121
Fragments arranged by size:
1138 1121 993 685 191 98
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 432 1381 2296 2788 3050 432
Size: 949 915 492 262 1608
Fragments arranged by size:
1608 949 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4226
SphI G_CATG'C
Cuts at: 1039 1279 1779 1039
Size: 240 500 3486
Fragments arranged by size:
3486 500 240
SspI AAT'ATT
Cuts at: 1066 1066
Size: 4226
StuI AGG'CCT
Cuts at: 407 407
Size: 4226
StyI C'CwwG_G
Cuts at: 275 1360 1714 275
Size: 1085 354 2787
Fragments arranged by size:
2787 1085 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 522 1809 1942 2401 2610 522
Size: 1287 133 459 209 2138
Fragments arranged by size:
2138 1287 459 209 133
TatI w'GTAC_w
Cuts at: 985 3741 985
Size: 2756 1470
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 305 337 3743 3775 305
Size: 32 3406 32 756
Fragments arranged by size:
3406 756 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 1257 1257
Size: 4226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 740 1721 740
Size: 981 3245
XhoI C'TCGA_G
Cuts at: 432 432
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NdeI NgoMIV NspI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AlfI AloI ApaI
AscI AvrII BaeI BfrBI BglI BmgBI BmtI BsaXI
BsiWI BsmBI BsrGI BssHII BstEII BstZ17I ClaI CspCI
EcoNI FseI FspAI HindIII HpaI MfeI NcoI NheI
NotI NruI NsiI PacI PasI PshAI PsiI PspOMI
PsrI SacII SexAI SfiI SgrAI SnaBI SrfI SwaI
XmnI ZraI