Restriction map for
pF1KB7665
(Circular) (Six-base) MAPSORT of: pF1KB7665.seq Check: 8245 from: 1 to: 4124
pF1KB7665 4124 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 689 689
Size: 4124
AccI GT'mk_AC
Cuts at: 1162 1162
Size: 4124
Acc65I G'GTAC_C
Cuts at: 512 1140 512
Size: 628 3496
AclI AA'CG_TT
Cuts at: 3964 3964
Size: 4124
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 737 1949 2381 3128 3396 400
Size: 337 1212 432 747 268 1128
Fragments arranged by size:
1212 1128 747 432 337 268
AflIII A'CryG_T
Cuts at: 1476 2580 3557 1476
Size: 1104 977 2043
Fragments arranged by size:
2043 1104 977
AgeI A'CCGG_T
Cuts at: 2512 2512
Size: 4124
AlwNI CAG_nnn'CTG
Cuts at: 1429 2996 1429
Size: 1567 2557
ApaLI G'TGCA_C
Cuts at: 2894 2894
Size: 4124
ApoI r'AATT_y
Cuts at: 219 1128 3477 3733 219
Size: 909 2349 256 610
Fragments arranged by size:
2349 909 610 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4124
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
AvaI C'yCGr_G
Cuts at: 186 1144 186
Size: 958 3166
BamHI G'GATC_C
Cuts at: 546 1149 546
Size: 603 3521
BanI G'GyrC_C
Cuts at: 512 1140 1554 1802 1837 512
Size: 628 414 248 35 2799
Fragments arranged by size:
2799 628 414 248 35
BanII G_rGCy'C
Cuts at: 523 1138 2168 523
Size: 615 1030 2479
Fragments arranged by size:
2479 1030 615
BbeI G_GCGC'C
Cuts at: 1558 1806 1558
Size: 248 3876
BbsI GAAGACnn'nnnn_
Cuts at: 330 330
Size: 4124
BbvCI CC'TCA_GC
Cuts at: 1052 1052
Size: 4124
BciVI GTATCCnnnnn_n'
Cuts at: 2022 2783 4124 2022
Size: 761 1341 2022
Fragments arranged by size:
2022 1341 761
BclI T'GATC_A
Cuts at: 1644 1644
Size: 4124
BglI GCCn_nnn'nGGC
Cuts at: 925 925
Size: 4124
BglII A'GATC_T
Cuts at: 1639 3676 1639
Size: 2037 2087
BlpI GC'TnA_GC
Cuts at: 1236 1236
Size: 4124
Bme1580I G_kGCm'C
Cuts at: 1749 1842 2898 1749
Size: 93 1056 2975
Fragments arranged by size:
2975 1056 93
BmgBI CAC'GTC
Cuts at: 165 1043 165
Size: 878 3246
BmrI ACTGGGnnnn_n'
Cuts at: 669 1336 1417 1752 3896 669
Size: 667 81 335 2144 897
Fragments arranged by size:
2144 897 667 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 150 2491 150
Size: 2341 1783
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 682 682
Size: 4124
Bpu10I CC'TnA_GC
Cuts at: 517 1052 517
Size: 535 3589
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1300 2179 2671 2969 1300
Size: 879 492 298 2455
Fragments arranged by size:
2455 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 148 3828 148
Size: 3680 444
BsaAI yAC'GTr
Cuts at: 1477 1477
Size: 4124
BsaBI GATnn'nnATC
Cuts at: 6 1638 3675 6
Size: 1632 2037 455
Fragments arranged by size:
2037 1632 455
BsaHI Gr'CG_yC
Cuts at: 285 1555 1803 2505 4001 285
Size: 1270 248 702 1496 408
Fragments arranged by size:
1496 1270 702 408 248
BsaWI w'CCGG_w
Cuts at: 1313 1834 2512 2786 2933 3344 1313
Size: 521 678 274 147 411 2093
Fragments arranged by size:
2093 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 505 535 1103 1133 505
Size: 30 568 30 3496
Fragments arranged by size:
3496 568 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 139 531 775 139
Size: 392 244 3488
Fragments arranged by size:
3488 392 244
BseYI C'CCAG_C
Cuts at: 593 1549 2307 2884 3380 593
Size: 956 758 577 496 1337
Fragments arranged by size:
1337 956 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 914 914
Size: 4124
BsiEI CG_ry'CG
Cuts at: 104 1666 1712 2920 104
Size: 1562 46 1208 1308
Fragments arranged by size:
1562 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 523 1138 1916 2106 2898 523
Size: 615 778 190 792 1749
Fragments arranged by size:
1749 792 778 615 190
BsmI GAATG_Cn'
Cuts at: 830 961 830
Size: 131 3993
BsmBI CGTCTCn'nnnn_
Cuts at: 121 121
Size: 4124
Bsp1286I G_dGCh'C
Cuts at: 523 1138 1749 1842 1916 2106 2168 2898 523
Size: 615 611 93 74 190 62 730 1749
Fragments arranged by size:
1749 730 615 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3344 3344
Size: 4124
BspHI T'CATG_A
Cuts at: 470 3300 470
Size: 2830 1294
BspMI ACCTGCnnnn'nnnn_
Cuts at: 689 1174 1388 1690 2071 689
Size: 485 214 302 381 2742
Fragments arranged by size:
2742 485 381 302 214
BsrBI CCG'CTC
Cuts at: 50 645 2416 2470 3954 4119 50
Size: 595 1771 54 1484 165 55
Fragments arranged by size:
1771 1484 595 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2036 2036
Size: 4124
BsrFI r'CCGG_y
Cuts at: 2122 2303 2512 2122
Size: 181 209 3734
Fragments arranged by size:
3734 209 181
BssSI C'ACGA_G
Cuts at: 2395 2753 2395
Size: 358 3766
BstAPI GCAn_nnn'nTGC
Cuts at: 1501 1501
Size: 4124
BstBI TT'CG_AA
Cuts at: 2485 2485
Size: 4124
BstXI CCAn_nnnn'nTGG
Cuts at: 165 165
Size: 4124
BstYI r'GATC_y
Cuts at: 1 355 546 1149 1616 1639 1974 2220 3232
Size: 354 191 603 467 23 335 246 1012
Cuts at: 3232 3318 3330 3676 1
Size: 86 12 346 449
Fragments arranged by size:
1012 603 467 449 354 346 335 246
191 86 23 12
BtgI C'CryG_G
Cuts at: 461 524 623 771 833 923 1001 461
Size: 63 99 148 62 90 78 3584
Fragments arranged by size:
3584 148 99 90 78 63 62
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2074 2255 2418 2074
Size: 181 163 3780
Fragments arranged by size:
3780 181 163
BtsI GCAGTG_nn'
Cuts at: 1001 1071 1367 1001
Size: 70 296 3758
Fragments arranged by size:
3758 296 70
DraI TTT'AAA
Cuts at: 1123 1123
Size: 4124
DrdI GACnn_nn'nnGTC
Cuts at: 1830 2688 1830
Size: 858 3266
EaeI y'GGCC_r
Cuts at: 608 791 998 1004 1709 1883 2274 2301 3646
Size: 183 207 6 705 174 391 27 1345
Cuts at: 3646 608
Size: 1086
Fragments arranged by size:
1345 1086 705 391 207 183 174 27
6
EagI C'GGCC_G
Cuts at: 1709 1709
Size: 4124
EarI CTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2332 2642 2788 3934 2332
Size: 310 146 1146 2522
Fragments arranged by size:
2522 1146 310 146
EcoICRI GAG'CTC
Cuts at: 521 1136 521
Size: 615 3509
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 400 682 737 1949 2381 3128 3396 400
Size: 282 55 1212 432 747 268 1128
Fragments arranged by size:
1212 1128 747 432 282 268 55
EcoO109I rG'GnC_Cy
Cuts at: 1263 1263
Size: 4124
EcoRI G'AATT_C
Cuts at: 219 1128 219
Size: 909 3215
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2143 2143
Size: 4124
FspI TGC'GCA
Cuts at: 1905 1905
Size: 4124
HaeII r_GCGC'y
Cuts at: 117 1558 1806 2828 3807 117
Size: 1441 248 1022 979 434
Fragments arranged by size:
1441 1022 979 434 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 384 416 1966 1996 1998 2028 384
Size: 32 1550 30 2 30 2480
Fragments arranged by size:
2480 1550 32 30 30 2
HincII GTy'rAC
Cuts at: 728 1163 728
Size: 435 3689
KasI G'GCGC_C
Cuts at: 1554 1802 1554
Size: 248 3876
KpnI G_GTAC'C
Cuts at: 516 1144 516
Size: 628 3496
MluI A'CGCG_T
Cuts at: 3557 3557
Size: 4124
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 250 1089 2795 2979 250
Size: 839 1706 184 1395
Fragments arranged by size:
1706 1395 839 184
MscI TGG'CCA
Cuts at: 1885 1885
Size: 4124
MslI CAynn'nnrTG
Cuts at: 163 2113 2240 163
Size: 1950 127 2047
Fragments arranged by size:
2047 1950 127
MspA1I CmG'CkG
Cuts at: 552 597 925 1003 1235 1549 1909 2922 3167
Size: 45 328 78 232 314 360 1013 245
Cuts at: 3167 552
Size: 1509
Fragments arranged by size:
1509 1013 360 328 314 245 232 78
45
NaeI GCC'GGC
Cuts at: 2305 2305
Size: 4124
NarI GG'CG_CC
Cuts at: 1555 1803 1555
Size: 248 3876
NcoI C'CATG_G
Cuts at: 461 623 461
Size: 162 3962
NgoMIV G'CCGG_C
Cuts at: 2303 2303
Size: 4124
NspI r_CATG'y
Cuts at: 1177 1677 2584 1177
Size: 500 907 2717
Fragments arranged by size:
2717 907 500
PasI CC'CwG_GG
Cuts at: 1094 1094
Size: 4124
PciI A'CATG_T
Cuts at: 2580 2580
Size: 4124
PmeI GTTT'AAAC
Cuts at: 1123 1123
Size: 4124
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 169 201 3263 3295 169
Size: 32 3062 32 998
Fragments arranged by size:
3062 998 32 32
PstI C_TGCA'G
Cuts at: 1171 1856 1171
Size: 685 3439
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4124
PvuII CAG'CTG
Cuts at: 1549 1909 1549
Size: 360 3764
RsrII CG'GwC_CG
Cuts at: 2319 2319
Size: 4124
SacI G_AGCT'C
Cuts at: 523 1138 523
Size: 615 3509
SacII CC_GC'GG
Cuts at: 926 1004 926
Size: 78 4046
SalI G'TCGA_C
Cuts at: 1161 1161
Size: 4124
SapI GCTCTTCn'nnn_
Cuts at: 2147 2357 2147
Size: 210 3914
SbfI CC_TGCA'GG
Cuts at: 1171 1171
Size: 4124
ScaI AGT'ACT
Cuts at: 198 3641 198
Size: 3443 681
SfcI C'TryA_G
Cuts at: 33 1167 1852 2845 3036 33
Size: 1134 685 993 191 1121
Fragments arranged by size:
1134 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1556 1804 1556
Size: 248 3876
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4124
SmaI CCC'GGG
Cuts at: 1146 1146
Size: 4124
SmlI C'TyrA_G
Cuts at: 1279 2194 2686 2948 1279
Size: 915 492 262 2455
Fragments arranged by size:
2455 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4124
SphI G_CATG'C
Cuts at: 1177 1677 1177
Size: 500 3624
SspI AAT'ATT
Cuts at: 383 383
Size: 4124
StuI AGG'CCT
Cuts at: 533 533
Size: 4124
StyI C'CwwG_G
Cuts at: 461 623 1258 1612 461
Size: 162 635 354 2973
Fragments arranged by size:
2973 635 354 162
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 607 1707 1840 2299 2508 607
Size: 1100 133 459 209 2223
Fragments arranged by size:
2223 1100 459 209 133
TatI w'GTAC_w
Cuts at: 196 3639 196
Size: 3443 681
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 923 955 1032 1064 3641 3673 923
Size: 32 77 32 2577 32 1374
Fragments arranged by size:
2577 1374 77 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1921 1921
Size: 4124
XbaI T'CTAG_A
Cuts at: 1155 1155
Size: 4124
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1619 1619
Size: 4124
XmaI C'CCGG_G
Cuts at: 1144 1144
Size: 4124
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI
PmeI PpiI PstI PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI ApaI
AscI AvrII BaeI BcgI BfrBI BmtI BsiWI BsrGI
BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoNI
EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PflMI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SanDI SexAI SfiI
SgrAI SnaBI SrfI SwaI XhoI XmnI ZraI