Restriction map for
pF1KB7661
(Circular) (Six-base) MAPSORT of: pF1KB7661.seq Check: 8891 from: 1 to: 4118
pF1KB7661 4118 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 459 459
Size: 4118
AccI GT'mk_AC
Cuts at: 1091 1156 1091
Size: 65 4053
Acc65I G'GTAC_C
Cuts at: 1134 1134
Size: 4118
AclI AA'CG_TT
Cuts at: 3958 3958
Size: 4118
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1943 2375 3122 3390 1943
Size: 432 747 268 2671
Fragments arranged by size:
2671 747 432 268
AfeI AGC'GCT
Cuts at: 688 688
Size: 4118
AflIII A'CryG_T
Cuts at: 1470 2574 3551 1470
Size: 1104 977 2037
Fragments arranged by size:
2037 1104 977
AgeI A'CCGG_T
Cuts at: 2506 2506
Size: 4118
AhdI GACnn_n'nnGTC
Cuts at: 174 174
Size: 4118
AleI CACnn'nnGTG
Cuts at: 785 785
Size: 4118
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 661 693 661
Size: 32 4086
AlwNI CAG_nnn'CTG
Cuts at: 270 323 639 1423 2990 270
Size: 53 316 784 1567 1398
Fragments arranged by size:
1567 1398 784 316 53
ApaLI G'TGCA_C
Cuts at: 1102 2888 1102
Size: 1786 2332
ApoI r'AATT_y
Cuts at: 1122 3471 3727 1122
Size: 2349 256 1513
Fragments arranged by size:
2349 1513 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4118
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
AvaI C'yCGr_G
Cuts at: 123 220 259 1138 123
Size: 97 39 879 3103
Fragments arranged by size:
3103 879 97 39
BamHI G'GATC_C
Cuts at: 1143 1143
Size: 4118
BanI G'GyrC_C
Cuts at: 429 1134 1548 1796 1831 429
Size: 705 414 248 35 2716
Fragments arranged by size:
2716 705 414 248 35
BanII G_rGCy'C
Cuts at: 562 1132 2162 562
Size: 570 1030 2518
Fragments arranged by size:
2518 1030 570
BbeI G_GCGC'C
Cuts at: 1552 1800 1552
Size: 248 3870
BciVI GTATCCnnnnn_n'
Cuts at: 150 2016 2777 4118 150
Size: 1866 761 1341 150
Fragments arranged by size:
1866 1341 761 150
BclI T'GATC_A
Cuts at: 1638 1638
Size: 4118
BglI GCCn_nnn'nGGC
Cuts at: 343 438 967 343
Size: 95 529 3494
Fragments arranged by size:
3494 529 95
BglII A'GATC_T
Cuts at: 1633 3670 1633
Size: 2037 2081
BlpI GC'TnA_GC
Cuts at: 1021 1230 1021
Size: 209 3909
Bme1580I G_kGCm'C
Cuts at: 388 434 1106 1743 1836 2892 388
Size: 46 672 637 93 1056 1614
Fragments arranged by size:
1614 1056 672 637 93 46
BmrI ACTGGGnnnn_n'
Cuts at: 236 1330 1411 1746 3890 236
Size: 1094 81 335 2144 464
Fragments arranged by size:
2144 1094 464 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 474 846 2485 474
Size: 372 1639 2107
Fragments arranged by size:
2107 1639 372
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 484 576 255
Size: 229 92 3797
Fragments arranged by size:
3797 229 92
Bpu10I CC'TnA_GC
Cuts at: 377 377
Size: 4118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 484 856 871 1294 2173 2665 2963 484
Size: 372 15 423 879 492 298 1639
Fragments arranged by size:
1639 879 492 423 372 298 15
BsaI GGTCTCn'nnnn_
Cuts at: 223 3822 223
Size: 3599 519
BsaAI yAC'GTr
Cuts at: 1471 1471
Size: 4118
BsaBI GATnn'nnATC
Cuts at: 6 1632 3669 6
Size: 1626 2037 455
Fragments arranged by size:
2037 1626 455
BsaHI Gr'CG_yC
Cuts at: 1549 1797 2499 3995 1549
Size: 248 702 1496 1672
Fragments arranged by size:
1672 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1061 1307 1828 2506 2780 2927 3338 1061
Size: 246 521 678 274 147 411 1841
Fragments arranged by size:
1841 678 521 411 274 246 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 228 258 661 691 228
Size: 30 403 30 3655
Fragments arranged by size:
3655 403 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 373 385 466 685 817 373
Size: 12 81 219 132 3674
Fragments arranged by size:
3674 219 132 81 12
BseYI C'CCAG_C
Cuts at: 145 323 408 562 1079 1543 2301 2878 3374
Size: 178 85 154 517 464 758 577 496
Cuts at: 3374 145
Size: 889
Fragments arranged by size:
889 758 577 517 496 464 178 154
85
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 493 592 697 493
Size: 99 105 3914
Fragments arranged by size:
3914 105 99
BsiEI CG_ry'CG
Cuts at: 104 1660 1706 2914 104
Size: 1556 46 1208 1308
Fragments arranged by size:
1556 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 458 500 599 1106 1132 1910 2100 2892 458
Size: 42 99 507 26 778 190 792 1684
Fragments arranged by size:
1684 792 778 507 190 99 42 26
BsmBI CGTCTCn'nnnn_
Cuts at: 219 505 219
Size: 286 3832
Bsp1286I G_dGCh'C
Cuts at: 388 434 458 500 562 599 1106 1132 1743
Size: 46 24 42 62 37 507 26 611
Cuts at: 1743 1836 1910 2100 2162 2892 388
Size: 93 74 190 62 730 1614
Fragments arranged by size:
1614 730 611 507 190 93 74 62
62 46 42 37 26 24
BspEI T'CCGG_A
Cuts at: 1061 3338 1061
Size: 2277 1841
BspHI T'CATG_A
Cuts at: 3294 3294
Size: 4118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 459 1168 1382 1684 2065 459
Size: 709 214 302 381 2512
Fragments arranged by size:
2512 709 381 302 214
BsrBI CCG'CTC
Cuts at: 50 759 1028 2410 2464 3948 4113 50
Size: 709 269 1382 54 1484 165 55
Fragments arranged by size:
1484 1382 709 269 165 55 54
BsrDI GCAATG_nn'
Cuts at: 573 2030 573
Size: 1457 2661
BsrFI r'CCGG_y
Cuts at: 605 2116 2297 2506 605
Size: 1511 181 209 2217
Fragments arranged by size:
2217 1511 209 181
BssSI C'ACGA_G
Cuts at: 2389 2747 2389
Size: 358 3760
BstAPI GCAn_nnn'nTGC
Cuts at: 1495 1495
Size: 4118
BstBI TT'CG_AA
Cuts at: 2479 2479
Size: 4118
BstXI CCAn_nnnn'nTGG
Cuts at: 486 901 486
Size: 415 3703
BstYI r'GATC_y
Cuts at: 1 543 1143 1610 1633 1968 2214 3226 3312
Size: 542 600 467 23 335 246 1012 86
Cuts at: 3312 3324 3670 1
Size: 12 346 449
Fragments arranged by size:
1012 600 542 467 449 346 335 246
86 23 12
Bsu36I CC'TnA_GG
Cuts at: 311 368 311
Size: 57 4061
BtgI C'CryG_G
Cuts at: 107 479 548 617 107
Size: 372 69 69 3608
Fragments arranged by size:
3608 372 69 69
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2068 2249 2412 2068
Size: 181 163 3774
Fragments arranged by size:
3774 181 163
BtsI GCAGTG_nn'
Cuts at: 218 388 1361 218
Size: 170 973 2975
Fragments arranged by size:
2975 973 170
DraI TTT'AAA
Cuts at: 1117 1117
Size: 4118
DrdI GACnn_nn'nnGTC
Cuts at: 1824 2682 1824
Size: 858 3260
EaeI y'GGCC_r
Cuts at: 903 1703 1877 2268 2295 3640 903
Size: 800 174 391 27 1345 1381
Fragments arranged by size:
1381 1345 800 391 174 27
EagI C'GGCC_G
Cuts at: 1703 1703
Size: 4118
EarI CTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 264 670 1024 2326 2636 2782 3928 264
Size: 406 354 1302 310 146 1146 454
Fragments arranged by size:
1302 1146 454 406 354 310 146
EcoICRI GAG'CTC
Cuts at: 1130 1130
Size: 4118
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 255 484 576 1943 2375 3122 3390 255
Size: 229 92 1367 432 747 268 983
Fragments arranged by size:
1367 983 747 432 268 229 92
EcoO109I rG'GnC_Cy
Cuts at: 267 336 1257 267
Size: 69 921 3128
Fragments arranged by size:
3128 921 69
EcoRI G'AATT_C
Cuts at: 1122 1122
Size: 4118
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 957 2137 957
Size: 1180 2938
FspI TGC'GCA
Cuts at: 1899 1899
Size: 4118
HaeII r_GCGC'y
Cuts at: 119 690 1086 1552 1800 2822 3801 119
Size: 571 396 466 248 1022 979 436
Fragments arranged by size:
1022 979 571 466 436 396 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 160 192 228 260 1960 1990 1992 2022 160
Size: 32 36 32 1700 30 2 30 2256
Fragments arranged by size:
2256 1700 36 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1157 1157
Size: 4118
KasI G'GCGC_C
Cuts at: 1548 1796 1548
Size: 248 3870
KpnI G_GTAC'C
Cuts at: 1138 1138
Size: 4118
MluI A'CGCG_T
Cuts at: 3551 3551
Size: 4118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 647 2789 2973 647
Size: 2142 184 1792
Fragments arranged by size:
2142 1792 184
MscI TGG'CCA
Cuts at: 1879 1879
Size: 4118
MslI CAynn'nnrTG
Cuts at: 484 785 899 2107 2234 484
Size: 301 114 1208 127 2368
Fragments arranged by size:
2368 1208 301 127 114
MspA1I CmG'CkG
Cuts at: 205 323 412 742 1229 1543 1903 2916 3161
Size: 118 89 330 487 314 360 1013 245
Cuts at: 3161 205
Size: 1162
Fragments arranged by size:
1162 1013 487 360 330 314 245 118
89
NaeI GCC'GGC
Cuts at: 2299 2299
Size: 4118
NarI GG'CG_CC
Cuts at: 1549 1797 1549
Size: 248 3870
NcoI C'CATG_G
Cuts at: 107 479 548 617 107
Size: 372 69 69 3608
Fragments arranged by size:
3608 372 69 69
NgoMIV G'CCGG_C
Cuts at: 2297 2297
Size: 4118
NspI r_CATG'y
Cuts at: 303 1171 1671 2578 303
Size: 868 500 907 1843
Fragments arranged by size:
1843 907 868 500
PasI CC'CwG_GG
Cuts at: 340 696 340
Size: 356 3762
PciI A'CATG_T
Cuts at: 2574 2574
Size: 4118
PflMI CCAn_nnn'nTGG
Cuts at: 554 639 554
Size: 85 4033
PfoI T'CCnGG_A
Cuts at: 632 632
Size: 4118
PmeI GTTT'AAAC
Cuts at: 1117 1117
Size: 4118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 661 693 3257 3289 661
Size: 32 2564 32 1490
Fragments arranged by size:
2564 1490 32 32
PshAI GACnn'nnGTC
Cuts at: 236 236
Size: 4118
PstI C_TGCA'G
Cuts at: 1165 1850 1165
Size: 685 3433
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4118
PvuII CAG'CTG
Cuts at: 323 1543 1903 323
Size: 1220 360 2538
Fragments arranged by size:
2538 1220 360
RsrII CG'GwC_CG
Cuts at: 2313 2313
Size: 4118
SacI G_AGCT'C
Cuts at: 1132 1132
Size: 4118
SalI G'TCGA_C
Cuts at: 1155 1155
Size: 4118
SapI GCTCTTCn'nnn_
Cuts at: 2141 2351 2141
Size: 210 3908
SbfI CC_TGCA'GG
Cuts at: 1165 1165
Size: 4118
ScaI AGT'ACT
Cuts at: 3635 3635
Size: 4118
SfcI C'TryA_G
Cuts at: 33 1161 1846 2839 3030 33
Size: 1128 685 993 191 1121
Fragments arranged by size:
1128 1121 993 685 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 343 343
Size: 4118
SfoI GGC'GCC
Cuts at: 1550 1798 1550
Size: 248 3870
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4118
SmaI CCC'GGG
Cuts at: 125 1140 125
Size: 1015 3103
SmlI C'TyrA_G
Cuts at: 220 499 871 886 1273 2188 2680 2942 220
Size: 279 372 15 387 915 492 262 1396
Fragments arranged by size:
1396 915 492 387 372 279 262 15
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4118
SphI G_CATG'C
Cuts at: 1171 1671 1171
Size: 500 3618
StuI AGG'CCT
Cuts at: 628 628
Size: 4118
StyI C'CwwG_G
Cuts at: 107 332 446 479 548 617 1252 1606 107
Size: 225 114 33 69 69 635 354 2619
Fragments arranged by size:
2619 635 354 225 114 69 69 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 688 829 1701 1834 2293 2502 688
Size: 141 872 133 459 209 2304
Fragments arranged by size:
2304 872 459 209 141 133
TatI w'GTAC_w
Cuts at: 3633 3633
Size: 4118
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 528 560 3635 3667 528
Size: 32 3075 32 979
Fragments arranged by size:
3075 979 32 32
Tth111I GACn'n_nGTC
Cuts at: 1915 1915
Size: 4118
XbaI T'CTAG_A
Cuts at: 1149 1149
Size: 4118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1613 1613
Size: 4118
XhoI C'TCGA_G
Cuts at: 220 220
Size: 4118
XmaI C'CCGG_G
Cuts at: 123 1138 123
Size: 1015 3103
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbeI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PstI PvuI
PvuII RsrII SacI SalI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI
Enzymes that do not cut:
AatII AflII AlfI ApaI AscI AvrII BaeI BbsI
BbvCI BcgI BfrBI BmgBI BmtI BsiWI BsmI BsrGI
BssHII BstEII BstZ17I ClaI CspCI DraIII EcoNI EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI NsiI PacI PmlI PpuMI PsiI PspOMI PspXI
PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI
SwaI XmnI ZraI