Restriction map for
pF1KB7660
(Circular) (Six-base) MAPSORT of: pF1KB7660.seq Check: 6576 from: 1 to: 4298
pF1KB7660 4298 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 858 858
Size: 4298
AccI GT'mk_AC
Cuts at: 821 1336 821
Size: 515 3783
Acc65I G'GTAC_C
Cuts at: 1314 1314
Size: 4298
AclI AA'CG_TT
Cuts at: 4138 4138
Size: 4298
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1159 2123 2555 3302 3570 1159
Size: 964 432 747 268 1887
Fragments arranged by size:
1887 964 747 432 268
AflIII A'CryG_T
Cuts at: 379 1650 2754 3731 379
Size: 1271 1104 977 946
Fragments arranged by size:
1271 1104 977 946
AgeI A'CCGG_T
Cuts at: 706 2686 706
Size: 1980 2318
AlwNI CAG_nnn'CTG
Cuts at: 700 1603 3170 700
Size: 903 1567 1828
Fragments arranged by size:
1828 1567 903
ApaI G_GGCC'C
Cuts at: 172 224 309 550 1268 1286 172
Size: 52 85 241 718 18 3184
Fragments arranged by size:
3184 718 241 85 52 18
ApaLI G'TGCA_C
Cuts at: 3068 3068
Size: 4298
ApoI r'AATT_y
Cuts at: 440 1302 3651 3907 440
Size: 862 2349 256 831
Fragments arranged by size:
2349 862 831 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4298
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
AvaI C'yCGr_G
Cuts at: 728 1245 1318 728
Size: 517 73 3708
Fragments arranged by size:
3708 517 73
BamHI G'GATC_C
Cuts at: 192 1323 192
Size: 1131 3167
BanI G'GyrC_C
Cuts at: 274 460 514 630 870 1314 1728 1976 2011
Size: 186 54 116 240 444 414 248 35
Cuts at: 2011 274
Size: 2561
Fragments arranged by size:
2561 444 414 248 240 186 116 54
35
BanII G_rGCy'C
Cuts at: 172 181 224 251 309 413 550 781 1268
Size: 9 43 27 58 104 137 231 487
Cuts at: 1268 1286 1312 2342 172
Size: 18 26 1030 2128
Fragments arranged by size:
2128 1030 487 231 137 104 58 43
27 26 18 9
BbeI G_GCGC'C
Cuts at: 464 518 1732 1980 464
Size: 54 1214 248 2782
Fragments arranged by size:
2782 1214 248 54
BciVI GTATCCnnnnn_n'
Cuts at: 2196 2957 4298 2196
Size: 761 1341 2196
Fragments arranged by size:
2196 1341 761
BclI T'GATC_A
Cuts at: 1818 1818
Size: 4298
BglI GCCn_nnn'nGGC
Cuts at: 1055 1281 1055
Size: 226 4072
BglII A'GATC_T
Cuts at: 472 1813 3850 472
Size: 1341 2037 920
Fragments arranged by size:
2037 1341 920
BlpI GC'TnA_GC
Cuts at: 1232 1410 1232
Size: 178 4120
Bme1580I G_kGCm'C
Cuts at: 172 224 277 309 550 635 724 875 1268
Size: 52 53 32 241 85 89 151 393
Cuts at: 1268 1286 1923 2016 3072 172
Size: 18 637 93 1056 1398
Fragments arranged by size:
1398 1056 637 393 241 151 93 89
85 53 52 32 18
BmgBI CAC'GTC
Cuts at: 380 380
Size: 4298
BmrI ACTGGGnnnn_n'
Cuts at: 617 625 1510 1591 1926 4070 617
Size: 8 885 81 335 2144 845
Fragments arranged by size:
2144 885 845 335 81 8
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2665 2665
Size: 4298
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 454 836 929 1201 427
Size: 27 382 93 272 3524
Fragments arranged by size:
3524 382 272 93 27
Bpu10I CC'TnA_GC
Cuts at: 780 780
Size: 4298
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1474 2353 2845 3143 1474
Size: 879 492 298 2629
Fragments arranged by size:
2629 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 395 697 4002 395
Size: 302 3305 691
Fragments arranged by size:
3305 691 302
BsaAI yAC'GTr
Cuts at: 1651 1651
Size: 4298
BsaBI GATnn'nnATC
Cuts at: 6 1812 3849 6
Size: 1806 2037 455
Fragments arranged by size:
2037 1806 455
BsaHI Gr'CG_yC
Cuts at: 461 515 1729 1977 2679 4175 461
Size: 54 1214 248 702 1496 584
Fragments arranged by size:
1496 1214 702 584 248 54
BsaWI w'CCGG_w
Cuts at: 706 1042 1487 2008 2686 2960 3107 3518 706
Size: 336 445 521 678 274 147 411 1486
Fragments arranged by size:
1486 678 521 445 411 336 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 663 1138 1233 663
Size: 475 95 3728
Fragments arranged by size:
3728 475 95
BseYI C'CCAG_C
Cuts at: 250 634 680 772 892 900 1723 2481 3058
Size: 384 46 92 120 8 823 758 577
Cuts at: 3058 3554 250
Size: 496 994
Fragments arranged by size:
994 823 758 577 496 384 120 92
46 8
BsiEI CG_ry'CG
Cuts at: 104 372 1840 1886 3094 104
Size: 268 1468 46 1208 1308
Fragments arranged by size:
1468 1308 1208 268 46
BsiHKAI G_wGCw'C
Cuts at: 852 1312 2090 2280 3072 852
Size: 460 778 190 792 2078
Fragments arranged by size:
2078 792 778 460 190
Bsp1286I G_dGCh'C
Cuts at: 172 181 224 251 277 309 413 550 635
Size: 9 43 27 26 32 104 137 85
Cuts at: 635 724 781 852 875 1268 1286 1312 1923
Size: 89 57 71 23 393 18 26 611
Cuts at: 1923 2016 2090 2280 2342 3072 172
Size: 93 74 190 62 730 1398
Fragments arranged by size:
1398 730 611 393 190 137 104 93
89 85 74 71 62 57 43 32
27 26 26 23 18 9
BspEI T'CCGG_A
Cuts at: 1042 3518 1042
Size: 2476 1822
BspHI T'CATG_A
Cuts at: 3474 3474
Size: 4298
BspMI ACCTGCnnnn'nnnn_
Cuts at: 858 1348 1562 1864 2245 858
Size: 490 214 302 381 2911
Fragments arranged by size:
2911 490 381 302 214
BsrBI CCG'CTC
Cuts at: 50 113 599 1167 2590 2644 4128 4293 50
Size: 63 486 568 1423 54 1484 165 55
Fragments arranged by size:
1484 1423 568 486 165 63 55 54
BsrDI GCAATG_nn'
Cuts at: 2210 2210
Size: 4298
BsrFI r'CCGG_y
Cuts at: 706 2296 2477 2686 706
Size: 1590 181 209 2318
Fragments arranged by size:
2318 1590 209 181
BssHII G'CGCG_C
Cuts at: 1203 1203
Size: 4298
BssSI C'ACGA_G
Cuts at: 2569 2927 2569
Size: 358 3940
BstAPI GCAn_nnn'nTGC
Cuts at: 1675 1675
Size: 4298
BstBI TT'CG_AA
Cuts at: 2659 2659
Size: 4298
BstEII G'GTnAC_C
Cuts at: 1012 1012
Size: 4298
BstXI CCAn_nnnn'nTGG
Cuts at: 1089 1089
Size: 4298
BstYI r'GATC_y
Cuts at: 1 192 472 1148 1323 1790 1813 2148 2394
Size: 191 280 676 175 467 23 335 246
Cuts at: 2394 3406 3492 3504 3850 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 676 467 449 346 335 280 246
191 175 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 453 590 453
Size: 137 4161
BtgI C'CryG_G
Cuts at: 155 342 366 1082 1255 155
Size: 187 24 716 173 3198
Fragments arranged by size:
3198 716 187 173 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2248 2429 2592 2248
Size: 181 163 3954
Fragments arranged by size:
3954 181 163
BtsI GCAGTG_nn'
Cuts at: 1541 1541
Size: 4298
DraI TTT'AAA
Cuts at: 1297 1297
Size: 4298
DrdI GACnn_nn'nnGTC
Cuts at: 2004 2862 2004
Size: 858 3440
EaeI y'GGCC_r
Cuts at: 497 1001 1211 1883 2057 2448 2475 3820 497
Size: 504 210 672 174 391 27 1345 975
Fragments arranged by size:
1345 975 672 504 391 210 174 27
EagI C'GGCC_G
Cuts at: 1883 1883
Size: 4298
EarI CTCTTCn'nnn_
Cuts at: 121 851 2321 2531 121
Size: 730 1470 210 1888
Fragments arranged by size:
1888 1470 730 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 440 1177 2506 2816 2962 4108 440
Size: 737 1329 310 146 1146 630
Fragments arranged by size:
1329 1146 737 630 310 146
EcoICRI GAG'CTC
Cuts at: 1310 1310
Size: 4298
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 427 454 836 929 1159 1201 2123 2555 3302
Size: 27 382 93 230 42 922 432 747
Cuts at: 3302 3570 427
Size: 268 1155
Fragments arranged by size:
1155 922 747 432 382 268 230 93
42 27
EcoNI CCTnn'n_nnAGG
Cuts at: 541 753 541
Size: 212 4086
EcoO109I rG'GnC_Cy
Cuts at: 168 169 221 305 306 449 546 547 791
Size: 1 52 84 1 143 97 1 244
Cuts at: 791 1251 1265 1283 1437 168
Size: 460 14 18 154 3029
Fragments arranged by size:
3029 460 244 154 143 97 84 52
18 14 1 1 1
EcoRI G'AATT_C
Cuts at: 440 1302 440
Size: 862 3436
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2317 2317
Size: 4298
FspI TGC'GCA
Cuts at: 2079 2079
Size: 4298
HaeII r_GCGC'y
Cuts at: 464 518 690 1732 1980 3002 3981 464
Size: 54 172 1042 248 1022 979 781
Fragments arranged by size:
1042 1022 979 781 248 172 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1024 1056 2140 2170 2172 2202 1024
Size: 32 1084 30 2 30 3120
Fragments arranged by size:
3120 1084 32 30 30 2
HincII GTy'rAC
Cuts at: 1337 1337
Size: 4298
KasI G'GCGC_C
Cuts at: 460 514 1728 1976 460
Size: 54 1214 248 2782
Fragments arranged by size:
2782 1214 248 54
KpnI G_GTAC'C
Cuts at: 1318 1318
Size: 4298
MluI A'CGCG_T
Cuts at: 3731 3731
Size: 4298
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 470 2969 3153 470
Size: 2499 184 1615
Fragments arranged by size:
2499 1615 184
MscI TGG'CCA
Cuts at: 499 1003 1213 2059 499
Size: 504 210 846 2738
Fragments arranged by size:
2738 846 504 210
MslI CAynn'nnrTG
Cuts at: 2287 2414 2287
Size: 127 4171
MspA1I CmG'CkG
Cuts at: 368 624 1227 1236 1409 1723 2083 3096 3341
Size: 256 603 9 173 314 360 1013 245
Cuts at: 3341 368
Size: 1325
Fragments arranged by size:
1325 1013 603 360 314 256 245 173
9
NaeI GCC'GGC
Cuts at: 2479 2479
Size: 4298
NarI GG'CG_CC
Cuts at: 461 515 1729 1977 461
Size: 54 1214 248 2782
Fragments arranged by size:
2782 1214 248 54
NcoI C'CATG_G
Cuts at: 1082 1082
Size: 4298
NgoMIV G'CCGG_C
Cuts at: 2477 2477
Size: 4298
NspI r_CATG'y
Cuts at: 828 1351 1851 2758 828
Size: 523 500 907 2368
Fragments arranged by size:
2368 907 523 500
PasI CC'CwG_GG
Cuts at: 172 181 172
Size: 9 4289
PciI A'CATG_T
Cuts at: 2754 2754
Size: 4298
PflMI CCAn_nnn'nTGG
Cuts at: 301 348 557 627 1261 301
Size: 47 209 70 634 3338
Fragments arranged by size:
3338 634 209 70 47
PfoI T'CCnGG_A
Cuts at: 431 582 805 1118 1151 431
Size: 151 223 313 33 3578
Fragments arranged by size:
3578 313 223 151 33
PmeI GTTT'AAAC
Cuts at: 1297 1297
Size: 4298
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3437 3469 3437
Size: 32 4266
PpuMI rG'GwC_Cy
Cuts at: 791 1251 791
Size: 460 3838
PspOMI G'GGCC_C
Cuts at: 168 220 305 546 1264 1282 168
Size: 52 85 241 718 18 3184
Fragments arranged by size:
3184 718 241 85 52 18
PstI C_TGCA'G
Cuts at: 1345 2030 1345
Size: 685 3613
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4298
PvuII CAG'CTG
Cuts at: 624 1227 1236 1723 2083 624
Size: 603 9 487 360 2839
Fragments arranged by size:
2839 603 487 360 9
RsrII CG'GwC_CG
Cuts at: 1116 2493 1116
Size: 1377 2921
SacI G_AGCT'C
Cuts at: 1312 1312
Size: 4298
SacII CC_GC'GG
Cuts at: 369 369
Size: 4298
SalI G'TCGA_C
Cuts at: 1335 1335
Size: 4298
SanDI GG'GwC_CC
Cuts at: 1251 1251
Size: 4298
SapI GCTCTTCn'nnn_
Cuts at: 2321 2531 2321
Size: 210 4088
SbfI CC_TGCA'GG
Cuts at: 1345 1345
Size: 4298
ScaI AGT'ACT
Cuts at: 3815 3815
Size: 4298
SfcI C'TryA_G
Cuts at: 33 818 1341 2026 3019 3210 33
Size: 785 523 685 993 191 1121
Fragments arranged by size:
1121 993 785 685 523 191
SfoI GGC'GCC
Cuts at: 462 516 1730 1978 462
Size: 54 1214 248 2782
Fragments arranged by size:
2782 1214 248 54
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4298
SmaI CCC'GGG
Cuts at: 730 1320 730
Size: 590 3708
SmlI C'TyrA_G
Cuts at: 1453 2368 2860 3122 1453
Size: 915 492 262 2629
Fragments arranged by size:
2629 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4298
SphI G_CATG'C
Cuts at: 1351 1851 1351
Size: 500 3798
StuI AGG'CCT
Cuts at: 536 536
Size: 4298
StyI C'CwwG_G
Cuts at: 644 1082 1432 1786 644
Size: 438 350 354 3156
Fragments arranged by size:
3156 438 354 350
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 835 931 1881 2014 2473 2682 835
Size: 96 950 133 459 209 2451
Fragments arranged by size:
2451 950 459 209 133 96
TatI w'GTAC_w
Cuts at: 3813 3813
Size: 4298
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3815 3847 3815
Size: 32 4266
Tth111I GACn'n_nGTC
Cuts at: 2095 2095
Size: 4298
XbaI T'CTAG_A
Cuts at: 1329 1329
Size: 4298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 492 507 1793 492
Size: 15 1286 2997
Fragments arranged by size:
2997 1286 15
XmaI C'CCGG_G
Cuts at: 728 1318 728
Size: 590 3708
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AlwNI
ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AfeI AflII AhdI AleI AlfI AloI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI BsaXI
BsgI BsiWI BsmI BsmBI BsrGI BstZ17I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PmlI PshAI PsiI
PspXI PsrI SexAI SfiI SgrAI SnaBI SrfI SspI
SwaI XhoI XmnI ZraI