Restriction map for
pF1KB7657
(Circular) (Six-base) MAPSORT of: pF1KB7657.seq Check: 9951 from: 1 to: 4115
pF1KB7657 4115 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 468 468
Size: 4115
AccI GT'mk_AC
Cuts at: 1153 1153
Size: 4115
Acc65I G'GTAC_C
Cuts at: 1131 1131
Size: 4115
AclI AA'CG_TT
Cuts at: 3955 3955
Size: 4115
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 349 722 1940 2372 3119 3387 349
Size: 373 1218 432 747 268 1077
Fragments arranged by size:
1218 1077 747 432 373 268
AfeI AGC'GCT
Cuts at: 183 183
Size: 4115
AflIII A'CryG_T
Cuts at: 1467 2571 3548 1467
Size: 1104 977 2034
Fragments arranged by size:
2034 1104 977
AgeI A'CCGG_T
Cuts at: 163 504 2503 163
Size: 341 1999 1775
Fragments arranged by size:
1999 1775 341
AlwNI CAG_nnn'CTG
Cuts at: 790 1420 2987 790
Size: 630 1567 1918
Fragments arranged by size:
1918 1567 630
ApaLI G'TGCA_C
Cuts at: 2885 2885
Size: 4115
ApoI r'AATT_y
Cuts at: 1119 3468 3724 1119
Size: 2349 256 1510
Fragments arranged by size:
2349 1510 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4115
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
AvaI C'yCGr_G
Cuts at: 260 329 775 1135 260
Size: 69 446 360 3240
Fragments arranged by size:
3240 446 360 69
BamHI G'GATC_C
Cuts at: 1140 1140
Size: 4115
BanI G'GyrC_C
Cuts at: 377 969 1068 1131 1545 1793 1828 377
Size: 592 99 63 414 248 35 2664
Fragments arranged by size:
2664 592 414 248 99 63 35
BanII G_rGCy'C
Cuts at: 782 1129 2159 782
Size: 347 1030 2738
Fragments arranged by size:
2738 1030 347
BbeI G_GCGC'C
Cuts at: 381 1549 1797 381
Size: 1168 248 2699
Fragments arranged by size:
2699 1168 248
BbsI GAAGACnn'nnnn_
Cuts at: 291 596 713 291
Size: 305 117 3693
Fragments arranged by size:
3693 305 117
BciVI GTATCCnnnnn_n'
Cuts at: 2013 2774 4115 2013
Size: 761 1341 2013
Fragments arranged by size:
2013 1341 761
BclI T'GATC_A
Cuts at: 1635 1635
Size: 4115
BglI GCCn_nnn'nGGC
Cuts at: 334 334
Size: 4115
BglII A'GATC_T
Cuts at: 731 1630 3667 731
Size: 899 2037 1179
Fragments arranged by size:
2037 1179 899
BlpI GC'TnA_GC
Cuts at: 1227 1227
Size: 4115
Bme1580I G_kGCm'C
Cuts at: 1073 1740 1833 2889 1073
Size: 667 93 1056 2299
Fragments arranged by size:
2299 1056 667 93
BmrI ACTGGGnnnn_n'
Cuts at: 1327 1408 1743 3887 1327
Size: 81 335 2144 1555
Fragments arranged by size:
2144 1555 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2482 2482
Size: 4115
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 289 599 941 1096 198
Size: 91 310 342 155 3217
Fragments arranged by size:
3217 342 310 155 91
Bpu10I CC'TnA_GC
Cuts at: 568 568
Size: 4115
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 1291 2170 2662 2960 298
Size: 993 879 492 298 1453
Fragments arranged by size:
1453 993 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 973 3819 973
Size: 2846 1269
BsaAI yAC'GTr
Cuts at: 1468 1468
Size: 4115
BsaBI GATnn'nnATC
Cuts at: 6 1629 3666 6
Size: 1623 2037 455
Fragments arranged by size:
2037 1623 455
BsaHI Gr'CG_yC
Cuts at: 245 378 1546 1794 2496 3992 245
Size: 133 1168 248 702 1496 368
Fragments arranged by size:
1496 1168 702 368 248 133
BsaWI w'CCGG_w
Cuts at: 163 504 1304 1825 2503 2777 2924 3335 163
Size: 341 800 521 678 274 147 411 943
Fragments arranged by size:
943 800 678 521 411 341 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 847 991 847
Size: 144 3971
BseYI C'CCAG_C
Cuts at: 396 905 987 1039 1540 2298 2875 3371 396
Size: 509 82 52 501 758 577 496 1140
Fragments arranged by size:
1140 758 577 509 501 496 82 52
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 502 611 837 502
Size: 109 226 3780
Fragments arranged by size:
3780 226 109
BsiEI CG_ry'CG
Cuts at: 104 1657 1703 2911 104
Size: 1553 46 1208 1308
Fragments arranged by size:
1553 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 485 782 1010 1129 1907 2097 2889 485
Size: 297 228 119 778 190 792 1711
Fragments arranged by size:
1711 792 778 297 228 190 119
BsmBI CGTCTCn'nnnn_
Cuts at: 874 874
Size: 4115
Bsp1286I G_dGCh'C
Cuts at: 485 782 1010 1073 1129 1740 1833 1907 2097
Size: 297 228 63 56 611 93 74 190
Cuts at: 2097 2159 2889 485
Size: 62 730 1711
Fragments arranged by size:
1711 730 611 297 228 190 93 74
63 62 56
BspEI T'CCGG_A
Cuts at: 3335 3335
Size: 4115
BspHI T'CATG_A
Cuts at: 3291 3291
Size: 4115
BspMI ACCTGCnnnn'nnnn_
Cuts at: 468 821 1165 1379 1681 2062 468
Size: 353 344 214 302 381 2521
Fragments arranged by size:
2521 381 353 344 302 214
BsrBI CCG'CTC
Cuts at: 50 133 2407 2461 3945 4110 50
Size: 83 2274 54 1484 165 55
Fragments arranged by size:
2274 1484 165 83 55 54
BsrDI GCAATG_nn'
Cuts at: 2027 2027
Size: 4115
BsrFI r'CCGG_y
Cuts at: 163 504 2113 2294 2503 163
Size: 341 1609 181 209 1775
Fragments arranged by size:
1775 1609 341 209 181
BssHII G'CGCG_C
Cuts at: 142 142
Size: 4115
BssSI C'ACGA_G
Cuts at: 2386 2744 2386
Size: 358 3757
BstAPI GCAn_nnn'nTGC
Cuts at: 204 1492 204
Size: 1288 2827
BstBI TT'CG_AA
Cuts at: 2476 2476
Size: 4115
BstEII G'GTnAC_C
Cuts at: 159 507 159
Size: 348 3767
BstXI CCAn_nnnn'nTGG
Cuts at: 255 255
Size: 4115
BstYI r'GATC_y
Cuts at: 1 290 420 731 1043 1140 1607 1630 1965
Size: 289 130 311 312 97 467 23 335
Cuts at: 1965 2211 3223 3309 3321 3667 1
Size: 246 1012 86 12 346 449
Fragments arranged by size:
1012 467 449 346 335 312 311 289
246 130 97 86 23 12
BtgI C'CryG_G
Cuts at: 497 549 497
Size: 52 4063
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 474 2065 2246 2409 474
Size: 1591 181 163 2180
Fragments arranged by size:
2180 1591 181 163
BtsI GCAGTG_nn'
Cuts at: 470 1358 470
Size: 888 3227
DraI TTT'AAA
Cuts at: 1114 1114
Size: 4115
DrdI GACnn_nn'nnGTC
Cuts at: 1821 2679 1821
Size: 858 3257
EaeI y'GGCC_r
Cuts at: 494 765 1700 1874 2265 2292 3637 494
Size: 271 935 174 391 27 1345 972
Fragments arranged by size:
1345 972 935 391 271 174 27
EagI C'GGCC_G
Cuts at: 1700 1700
Size: 4115
EarI CTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2323 2633 2779 3925 2323
Size: 310 146 1146 2513
Fragments arranged by size:
2513 1146 310 146
EcoICRI GAG'CTC
Cuts at: 780 1127 780
Size: 347 3768
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 198 289 349 599 722 941 1096 1940 2372
Size: 91 60 250 123 219 155 844 432
Cuts at: 2372 3119 3387 198
Size: 747 268 926
Fragments arranged by size:
926 844 747 432 268 250 219 155
123 91 60
EcoO109I rG'GnC_Cy
Cuts at: 934 1254 934
Size: 320 3795
EcoRI G'AATT_C
Cuts at: 1119 1119
Size: 4115
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2134 2134
Size: 4115
FspI TGC'GCA
Cuts at: 1896 1896
Size: 4115
HaeII r_GCGC'y
Cuts at: 185 381 575 1549 1797 2819 3798 185
Size: 196 194 974 248 1022 979 502
Fragments arranged by size:
1022 979 974 502 248 196 194
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1957 1987 1989 2019 1957
Size: 30 2 30 4053
Fragments arranged by size:
4053 30 30 2
HincII GTy'rAC
Cuts at: 1154 1154
Size: 4115
KasI G'GCGC_C
Cuts at: 377 1545 1793 377
Size: 1168 248 2699
Fragments arranged by size:
2699 1168 248
KpnI G_GTAC'C
Cuts at: 1135 1135
Size: 4115
MluI A'CGCG_T
Cuts at: 3548 3548
Size: 4115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2786 2970 2786
Size: 184 3931
MscI TGG'CCA
Cuts at: 1876 1876
Size: 4115
MslI CAynn'nnrTG
Cuts at: 356 2104 2231 356
Size: 1748 127 2240
Fragments arranged by size:
2240 1748 127
MspA1I CmG'CkG
Cuts at: 790 799 919 1039 1226 1540 1900 2913 3158
Size: 9 120 120 187 314 360 1013 245
Cuts at: 3158 790
Size: 1747
Fragments arranged by size:
1747 1013 360 314 245 187 120 120
9
NaeI GCC'GGC
Cuts at: 2296 2296
Size: 4115
NarI GG'CG_CC
Cuts at: 378 1546 1794 378
Size: 1168 248 2699
Fragments arranged by size:
2699 1168 248
NdeI CA'TA_TG
Cuts at: 872 872
Size: 4115
NgoMIV G'CCGG_C
Cuts at: 2294 2294
Size: 4115
NruI TCG'CGA
Cuts at: 188 188
Size: 4115
NspI r_CATG'y
Cuts at: 1168 1668 2575 1168
Size: 500 907 2708
Fragments arranged by size:
2708 907 500
PciI A'CATG_T
Cuts at: 2571 2571
Size: 4115
PmeI GTTT'AAAC
Cuts at: 1114 1114
Size: 4115
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3254 3286 3254
Size: 32 4083
PstI C_TGCA'G
Cuts at: 218 942 1162 1847 218
Size: 724 220 685 2486
Fragments arranged by size:
2486 724 685 220
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4115
PvuII CAG'CTG
Cuts at: 790 1039 1540 1900 790
Size: 249 501 360 3005
Fragments arranged by size:
3005 501 360 249
RsrII CG'GwC_CG
Cuts at: 2310 2310
Size: 4115
SacI G_AGCT'C
Cuts at: 782 1129 782
Size: 347 3768
SalI G'TCGA_C
Cuts at: 1152 1152
Size: 4115
SapI GCTCTTCn'nnn_
Cuts at: 2138 2348 2138
Size: 210 3905
SbfI CC_TGCA'GG
Cuts at: 218 1162 218
Size: 944 3171
ScaI AGT'ACT
Cuts at: 742 3632 742
Size: 2890 1225
SfcI C'TryA_G
Cuts at: 33 214 938 1158 1843 2836 3027 33
Size: 181 724 220 685 993 191 1121
Fragments arranged by size:
1121 993 724 685 220 191 181
SfoI GGC'GCC
Cuts at: 379 1547 1795 379
Size: 1168 248 2699
Fragments arranged by size:
2699 1168 248
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4115
SmaI CCC'GGG
Cuts at: 262 331 1137 262
Size: 69 806 3240
Fragments arranged by size:
3240 806 69
SmlI C'TyrA_G
Cuts at: 313 1270 2185 2677 2939 313
Size: 957 915 492 262 1489
Fragments arranged by size:
1489 957 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4115
SphI G_CATG'C
Cuts at: 1168 1668 1168
Size: 500 3615
StyI C'CwwG_G
Cuts at: 373 965 1027 1249 1603 373
Size: 592 62 222 354 2885
Fragments arranged by size:
2885 592 354 222 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 657 1698 1831 2290 2499 657
Size: 1041 133 459 209 2273
Fragments arranged by size:
2273 1041 459 209 133
TatI w'GTAC_w
Cuts at: 740 3630 740
Size: 2890 1225
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3632 3664 3632
Size: 32 4083
Tth111I GACn'n_nGTC
Cuts at: 1912 1912
Size: 4115
XbaI T'CTAG_A
Cuts at: 1146 1146
Size: 4115
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1610 1610
Size: 4115
XmaI C'CCGG_G
Cuts at: 260 329 1135 260
Size: 69 806 3240
Fragments arranged by size:
3240 806 69
XmnI GAAnn'nnTTC
Cuts at: 1060 1060
Size: 4115
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NdeI NgoMIV NruI NspI PciI PmeI
PpiI PstI PvuI PvuII RsrII SacI SalI SapI
SbfI ScaI SfcI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbvCI BcgI BfrBI BmgBI BmtI BsaXI
BsiWI BsmI BsrGI BstZ17I Bsu36I ClaI CspCI DraIII
EcoNI EcoRV FseI FspAI HindIII HpaI MfeI NcoI
NheI NotI NsiI PacI PasI PflMI PfoI PmlI
PpuMI PshAI PsiI PspOMI PspXI PsrI SacII SanDI
SexAI SfiI SgrAI SnaBI SrfI SspI StuI SwaI
XhoI ZraI