Restriction map for
pF1KB7656
(Circular) (Six-base) MAPSORT of: pF1KB7656.seq Check: 6154 from: 1 to: 4295
pF1KB7656 4295 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1333 1333
Size: 4295
Acc65I G'GTAC_C
Cuts at: 1311 1311
Size: 4295
AclI AA'CG_TT
Cuts at: 4135 4135
Size: 4295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 2120 2552 3299 3567 547
Size: 1573 432 747 268 1275
Fragments arranged by size:
1573 1275 747 432 268
AflIII A'CryG_T
Cuts at: 1647 2751 3728 1647
Size: 1104 977 2214
Fragments arranged by size:
2214 1104 977
AgeI A'CCGG_T
Cuts at: 2683 2683
Size: 4295
AlwNI CAG_nnn'CTG
Cuts at: 660 1600 3167 660
Size: 940 1567 1788
Fragments arranged by size:
1788 1567 940
ApaLI G'TGCA_C
Cuts at: 3065 3065
Size: 4295
ApoI r'AATT_y
Cuts at: 313 393 532 1299 3648 3904 313
Size: 80 139 767 2349 256 704
Fragments arranged by size:
2349 767 704 256 139 80
AseI AT'TA_AT
Cuts at: 20 20
Size: 4295
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
AvaI C'yCGr_G
Cuts at: 1024 1246 1315 1024
Size: 222 69 4004
Fragments arranged by size:
4004 222 69
BamHI G'GATC_C
Cuts at: 1320 1320
Size: 4295
BanI G'GyrC_C
Cuts at: 1259 1311 1725 1973 2008 1259
Size: 52 414 248 35 3546
Fragments arranged by size:
3546 414 248 52 35
BanII G_rGCy'C
Cuts at: 1309 2339 1309
Size: 1030 3265
BbeI G_GCGC'C
Cuts at: 1729 1977 1729
Size: 248 4047
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4261
BciVI GTATCCnnnnn_n'
Cuts at: 2193 2954 4295 2193
Size: 761 1341 2193
Fragments arranged by size:
2193 1341 761
BclI T'GATC_A
Cuts at: 572 1815 572
Size: 1243 3052
BglI GCCn_nnn'nGGC
Cuts at: 522 1283 522
Size: 761 3534
BglII A'GATC_T
Cuts at: 210 1810 3847 210
Size: 1600 2037 658
Fragments arranged by size:
2037 1600 658
BlpI GC'TnA_GC
Cuts at: 1407 1407
Size: 4295
Bme1580I G_kGCm'C
Cuts at: 1920 2013 3069 1920
Size: 93 1056 3146
Fragments arranged by size:
3146 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 666 1101 1507 1588 1923 4067 666
Size: 435 406 81 335 2144 894
Fragments arranged by size:
2144 894 435 406 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2662
Size: 4295
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 570 895 570
Size: 325 3970
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 235 1471 2350 2842 3140 235
Size: 1236 879 492 298 1390
Fragments arranged by size:
1390 1236 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 716 3999 716
Size: 3283 1012
BsaAI yAC'GTr
Cuts at: 261 1648 261
Size: 1387 2908
BsaBI GATnn'nnATC
Cuts at: 6 1809 3846 6
Size: 1803 2037 455
Fragments arranged by size:
2037 1803 455
BsaHI Gr'CG_yC
Cuts at: 1726 1974 2676 4172 1726
Size: 248 702 1496 1849
Fragments arranged by size:
1849 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1484 2005 2683 2957 3104 3515 1484
Size: 521 678 274 147 411 2264
Fragments arranged by size:
2264 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1170 1170
Size: 4295
BseYI C'CCAG_C
Cuts at: 1720 2478 3055 3551 1720
Size: 758 577 496 2464
Fragments arranged by size:
2464 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1837 1883 3091 104
Size: 1733 46 1208 1308
Fragments arranged by size:
1733 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1309 2087 2277 3069 1309
Size: 778 190 792 2535
Fragments arranged by size:
2535 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 1309 1920 2013 2087 2277 2339 3069 1309
Size: 611 93 74 190 62 730 2535
Fragments arranged by size:
2535 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3515 3515
Size: 4295
BspHI T'CATG_A
Cuts at: 1064 1133 3471 1064
Size: 69 2338 1888
Fragments arranged by size:
2338 1888 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1065 1345 1559 1861 2242 1065
Size: 280 214 302 381 3118
Fragments arranged by size:
3118 381 302 280 214
BsrBI CCG'CTC
Cuts at: 50 2587 2641 4125 4290 50
Size: 2537 54 1484 165 55
Fragments arranged by size:
2537 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 421 2207 421
Size: 1786 2509
BsrFI r'CCGG_y
Cuts at: 2293 2474 2683 2293
Size: 181 209 3905
Fragments arranged by size:
3905 209 181
BsrGI T'GTAC_A
Cuts at: 849 849
Size: 4295
BssSI C'ACGA_G
Cuts at: 838 2566 2924 838
Size: 1728 358 2209
Fragments arranged by size:
2209 1728 358
BstAPI GCAn_nnn'nTGC
Cuts at: 423 1672 423
Size: 1249 3046
BstBI TT'CG_AA
Cuts at: 363 2656 363
Size: 2293 2002
BstYI r'GATC_y
Cuts at: 1 210 1320 1787 1810 2145 2391 3403 3489
Size: 209 1110 467 23 335 246 1012 86
Cuts at: 3489 3501 3847 1
Size: 12 346 449
Fragments arranged by size:
1110 1012 467 449 346 335 246 209
86 23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2245 2426 2589 2245
Size: 181 163 3951
Fragments arranged by size:
3951 181 163
BtsI GCAGTG_nn'
Cuts at: 119 834 1538 119
Size: 715 704 2876
Fragments arranged by size:
2876 715 704
DraI TTT'AAA
Cuts at: 156 1235 1294 156
Size: 1079 59 3157
Fragments arranged by size:
3157 1079 59
DraIII CAC_nnn'GTG
Cuts at: 858 858
Size: 4295
DrdI GACnn_nn'nnGTC
Cuts at: 2001 2859 2001
Size: 858 3437
EaeI y'GGCC_r
Cuts at: 1275 1284 1880 2054 2445 2472 3817 1275
Size: 9 596 174 391 27 1345 1753
Fragments arranged by size:
1753 1345 596 391 174 27 9
EagI C'GGCC_G
Cuts at: 1880 1880
Size: 4295
EarI CTCTTCn'nnn_
Cuts at: 123 1132 1172 1178 1184 1196 2318 2528 123
Size: 1009 40 6 6 12 1122 210 1890
Fragments arranged by size:
1890 1122 1009 210 40 12 6 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 516 2503 2813 2959 4105 516
Size: 1987 310 146 1146 706
Fragments arranged by size:
1987 1146 706 310 146
EcoICRI GAG'CTC
Cuts at: 1307 1307
Size: 4295
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 547 570 895 2120 2552 3299 3567 547
Size: 23 325 1225 432 747 268 1275
Fragments arranged by size:
1275 1225 747 432 325 268 23
EcoO109I rG'GnC_Cy
Cuts at: 833 1434 833
Size: 601 3694
EcoRI G'AATT_C
Cuts at: 1299 1299
Size: 4295
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 477 2314 477
Size: 1837 2458
FspI TGC'GCA
Cuts at: 2076 2076
Size: 4295
HaeII r_GCGC'y
Cuts at: 474 1729 1977 2999 3978 474
Size: 1255 248 1022 979 791
Fragments arranged by size:
1255 1022 979 791 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 2137 2167 2169 2199 2137
Size: 30 2 30 4233
Fragments arranged by size:
4233 30 30 2
HincII GTy'rAC
Cuts at: 1334 1334
Size: 4295
HindIII A'AGCT_T
Cuts at: 542 542
Size: 4295
KasI G'GCGC_C
Cuts at: 1725 1973 1725
Size: 248 4047
KpnI G_GTAC'C
Cuts at: 1315 1315
Size: 4295
MluI A'CGCG_T
Cuts at: 3728 3728
Size: 4295
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 152 217 2966 3150 152
Size: 65 2749 184 1297
Fragments arranged by size:
2749 1297 184 65
MscI TGG'CCA
Cuts at: 1277 1286 2056 1277
Size: 9 770 3516
Fragments arranged by size:
3516 770 9
MslI CAynn'nnrTG
Cuts at: 1286 2284 2411 1286
Size: 998 127 3170
Fragments arranged by size:
3170 998 127
MspA1I CmG'CkG
Cuts at: 1406 1720 2080 3093 3338 1406
Size: 314 360 1013 245 2363
Fragments arranged by size:
2363 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2476 2476
Size: 4295
NarI GG'CG_CC
Cuts at: 1726 1974 1726
Size: 248 4047
NdeI CA'TA_TG
Cuts at: 273 273
Size: 4295
NgoMIV G'CCGG_C
Cuts at: 2474 2474
Size: 4295
NspI r_CATG'y
Cuts at: 1348 1848 2755 1348
Size: 500 907 2888
Fragments arranged by size:
2888 907 500
PciI A'CATG_T
Cuts at: 2751 2751
Size: 4295
PflMI CCAn_nnn'nTGG
Cuts at: 777 777
Size: 4295
PfoI T'CCnGG_A
Cuts at: 536 1145 536
Size: 609 3686
PmeI GTTT'AAAC
Cuts at: 1294 1294
Size: 4295
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3434 3466 3434
Size: 32 4263
PpuMI rG'GwC_Cy
Cuts at: 833 833
Size: 4295
PspXI vC'TCGA_Gb
Cuts at: 1024 1024
Size: 4295
PstI C_TGCA'G
Cuts at: 1342 2027 1342
Size: 685 3610
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4295
PvuII CAG'CTG
Cuts at: 1720 2080 1720
Size: 360 3935
RsrII CG'GwC_CG
Cuts at: 2490 2490
Size: 4295
SacI G_AGCT'C
Cuts at: 1309 1309
Size: 4295
SalI G'TCGA_C
Cuts at: 1332 1332
Size: 4295
SanDI GG'GwC_CC
Cuts at: 833 833
Size: 4295
SapI GCTCTTCn'nnn_
Cuts at: 123 1132 2318 2528 123
Size: 1009 1186 210 1890
Fragments arranged by size:
1890 1186 1009 210
SbfI CC_TGCA'GG
Cuts at: 1342 1342
Size: 4295
ScaI AGT'ACT
Cuts at: 676 3812 676
Size: 3136 1159
SfcI C'TryA_G
Cuts at: 33 1057 1338 2023 3016 3207 33
Size: 1024 281 685 993 191 1121
Fragments arranged by size:
1121 1024 993 685 281 191
SfiI GGCCn_nnn'nGGCC
Cuts at: 1283 1283
Size: 4295
SfoI GGC'GCC
Cuts at: 1727 1975 1727
Size: 248 4047
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4295
SmaI CCC'GGG
Cuts at: 1317 1317
Size: 4295
SmlI C'TyrA_G
Cuts at: 214 1024 1450 2365 2857 3119 214
Size: 810 426 915 492 262 1390
Fragments arranged by size:
1390 915 810 492 426 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4295
SphI G_CATG'C
Cuts at: 1348 1848 1348
Size: 500 3795
StuI AGG'CCT
Cuts at: 516 516
Size: 4295
StyI C'CwwG_G
Cuts at: 610 1429 1783 610
Size: 819 354 3122
Fragments arranged by size:
3122 819 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1878 2011 2470 2679 1878
Size: 133 459 209 3494
Fragments arranged by size:
3494 459 209 133
TatI w'GTAC_w
Cuts at: 674 849 3810 674
Size: 175 2961 1159
Fragments arranged by size:
2961 1159 175
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3812 3844 3812
Size: 32 4263
Tth111I GACn'n_nGTC
Cuts at: 2092 2092
Size: 4295
XbaI T'CTAG_A
Cuts at: 1326 1326
Size: 4295
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 200 674 1790 200
Size: 474 1116 2705
Fragments arranged by size:
2705 1116 474
XhoI C'TCGA_G
Cuts at: 1024 1024
Size: 4295
XmaI C'CCGG_G
Cuts at: 1315 1315
Size: 4295
XmnI GAAnn'nnTTC
Cuts at: 397 532 397
Size: 135 4160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BseYI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstYI
BtgZI BtsI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NspI PciI
PflMI PfoI PmeI PpiI PpuMI PspXI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AleI AlfI AloI
ApaI AscI AvrII BaeI BbsI BbvCI BfrBI BmgBI
BmtI Bpu10I BsaXI BsgI BsiWI BsmI BsmBI BssHII
BstEII BstXI BstZ17I Bsu36I BtgI ClaI CspCI EcoNI
EcoRV FseI FspAI HpaI MfeI NcoI NheI NotI
NruI NsiI PacI PasI PmlI PshAI PsiI PspOMI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI SwaI
ZraI