Restriction map for
pF1KB7654
(Circular) (Six-base) MAPSORT of: pF1KB7654.seq Check: 893 from: 1 to: 4226
pF1KB7654 4226 bp
With 182 enzymes: SgfI *
June 20, 2008 10:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 802 802
Size: 4226
AccI GT'mk_AC
Cuts at: 1264 1264
Size: 4226
Acc65I G'GTAC_C
Cuts at: 237 1242 237
Size: 1005 3221
AclI AA'CG_TT
Cuts at: 4066 4066
Size: 4226
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 2051 2483 3230 3498 667
Size: 1384 432 747 268 1395
Fragments arranged by size:
1395 1384 747 432 268
AflIII A'CryG_T
Cuts at: 564 1578 2682 3659 564
Size: 1014 1104 977 1131
Fragments arranged by size:
1131 1104 1014 977
AgeI A'CCGG_T
Cuts at: 2614 2614
Size: 4226
AleI CACnn'nnGTG
Cuts at: 984 984
Size: 4226
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 473 473
Size: 4226
AlwNI CAG_nnn'CTG
Cuts at: 702 794 1531 3098 702
Size: 92 737 1567 1830
Fragments arranged by size:
1830 1567 737 92
ApaI G_GGCC'C
Cuts at: 207 207
Size: 4226
ApaLI G'TGCA_C
Cuts at: 2996 2996
Size: 4226
ApoI r'AATT_y
Cuts at: 554 714 1230 3579 3835 554
Size: 160 516 2349 256 945
Fragments arranged by size:
2349 945 516 256 160
AseI AT'TA_AT
Cuts at: 20 20
Size: 4226
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
AvaI C'yCGr_G
Cuts at: 1246 1246
Size: 4226
BamHI G'GATC_C
Cuts at: 1251 1251
Size: 4226
BanI G'GyrC_C
Cuts at: 183 237 1242 1656 1904 1939 183
Size: 54 1005 414 248 35 2470
Fragments arranged by size:
2470 1005 414 248 54 35
BanII G_rGCy'C
Cuts at: 207 1240 2270 207
Size: 1033 1030 2163
Fragments arranged by size:
2163 1033 1030
BbeI G_GCGC'C
Cuts at: 1660 1908 1660
Size: 248 3978
BciVI GTATCCnnnnn_n'
Cuts at: 2124 2885 4226 2124
Size: 761 1341 2124
Fragments arranged by size:
2124 1341 761
BclI T'GATC_A
Cuts at: 1746 1746
Size: 4226
BglI GCCn_nnn'nGGC
Cuts at: 202 202
Size: 4226
BglII A'GATC_T
Cuts at: 592 1741 3778 592
Size: 1149 2037 1040
Fragments arranged by size:
2037 1149 1040
BlpI GC'TnA_GC
Cuts at: 878 1338 878
Size: 460 3766
Bme1580I G_kGCm'C
Cuts at: 207 912 1851 1944 3000 207
Size: 705 939 93 1056 1433
Fragments arranged by size:
1433 1056 939 705 93
BmrI ACTGGGnnnn_n'
Cuts at: 225 258 911 1058 1438 1519 1854 3998 225
Size: 33 653 147 380 81 335 2144 453
Fragments arranged by size:
2144 653 453 380 335 147 81 33
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2593 2593
Size: 4226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 724 730 958 997 1102 724
Size: 6 228 39 105 3848
Fragments arranged by size:
3848 228 105 39 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 584 1402 2281 2773 3071 584
Size: 818 879 492 298 1739
Fragments arranged by size:
1739 879 818 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3930 3930
Size: 4226
BsaAI yAC'GTr
Cuts at: 1579 1579
Size: 4226
BsaBI GATnn'nnATC
Cuts at: 6 1740 3777 6
Size: 1734 2037 455
Fragments arranged by size:
2037 1734 455
BsaHI Gr'CG_yC
Cuts at: 1657 1905 2607 4103 1657
Size: 248 702 1496 1780
Fragments arranged by size:
1780 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1415 1936 2614 2888 3035 3446 1415
Size: 521 678 274 147 411 2195
Fragments arranged by size:
2195 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 334 364 334
Size: 30 4196
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 303 451 496 817 840 303
Size: 148 45 321 23 3689
Fragments arranged by size:
3689 321 148 45 23
BseYI C'CCAG_C
Cuts at: 288 1651 2409 2986 3482 288
Size: 1363 758 577 496 1032
Fragments arranged by size:
1363 1032 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1088 1088
Size: 4226
BsiEI CG_ry'CG
Cuts at: 104 1768 1814 3022 104
Size: 1664 46 1208 1308
Fragments arranged by size:
1664 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 274 1240 2018 2208 3000 274
Size: 966 778 190 792 1500
Fragments arranged by size:
1500 966 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 207 274 912 1240 1851 1944 2018 2208 2270
Size: 67 638 328 611 93 74 190 62
Cuts at: 2270 3000 207
Size: 730 1433
Fragments arranged by size:
1433 730 638 611 328 190 93 74
67 62
BspEI T'CCGG_A
Cuts at: 3446 3446
Size: 4226
BspHI T'CATG_A
Cuts at: 3402 3402
Size: 4226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 149 802 846 1276 1490 1792 2173 149
Size: 653 44 430 214 302 381 2202
Fragments arranged by size:
2202 653 430 381 302 214 44
BsrBI CCG'CTC
Cuts at: 50 2518 2572 4056 4221 50
Size: 2468 54 1484 165 55
Fragments arranged by size:
2468 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2138 2138
Size: 4226
BsrFI r'CCGG_y
Cuts at: 2224 2405 2614 2224
Size: 181 209 3836
Fragments arranged by size:
3836 209 181
BssSI C'ACGA_G
Cuts at: 2497 2855 2497
Size: 358 3868
BstAPI GCAn_nnn'nTGC
Cuts at: 794 1050 1603 794
Size: 256 553 3417
Fragments arranged by size:
3417 553 256
BstBI TT'CG_AA
Cuts at: 582 2587 582
Size: 2005 2221
BstEII G'GTnAC_C
Cuts at: 130 130
Size: 4226
BstXI CCAn_nnnn'nTGG
Cuts at: 114 215 1021 114
Size: 101 806 3319
Fragments arranged by size:
3319 806 101
BstYI r'GATC_y
Cuts at: 1 378 592 1251 1718 1741 2076 2322 3334
Size: 377 214 659 467 23 335 246 1012
Cuts at: 3334 3420 3432 3778 1
Size: 86 12 346 449
Fragments arranged by size:
1012 659 467 449 377 346 335 246
214 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 1055 1055
Size: 4226
BtgI C'CryG_G
Cuts at: 107 358 752 107
Size: 251 394 3581
Fragments arranged by size:
3581 394 251
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2176 2357 2520 2176
Size: 181 163 3882
Fragments arranged by size:
3882 181 163
BtsI GCAGTG_nn'
Cuts at: 1469 1469
Size: 4226
DraI TTT'AAA
Cuts at: 1225 1225
Size: 4226
DrdI GACnn_nn'nnGTC
Cuts at: 1932 2790 1932
Size: 858 3368
EaeI y'GGCC_r
Cuts at: 1087 1811 1985 2376 2403 3748 1087
Size: 724 174 391 27 1345 1565
Fragments arranged by size:
1565 1345 724 391 174 27
EagI C'GGCC_G
Cuts at: 1811 1811
Size: 4226
EarI CTCTTCn'nnn_
Cuts at: 481 1108 2249 2459 481
Size: 627 1141 210 2248
Fragments arranged by size:
2248 1141 627 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2434 2744 2890 4036 2434
Size: 310 146 1146 2624
Fragments arranged by size:
2624 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1238 1238
Size: 4226
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 667 724 730 958 997 1102 2051 2483 3230
Size: 57 6 228 39 105 949 432 747
Cuts at: 3230 3498 667
Size: 268 1395
Fragments arranged by size:
1395 949 747 432 268 228 105 57
39 6
EcoNI CCTnn'n_nnAGG
Cuts at: 157 157
Size: 4226
EcoO109I rG'GnC_Cy
Cuts at: 203 204 410 924 1017 1126 1365 203
Size: 1 206 514 93 109 239 3064
Fragments arranged by size:
3064 514 239 206 109 93 1
EcoRI G'AATT_C
Cuts at: 1230 1230
Size: 4226
EcoRV GAT'ATC
Cuts at: 767 767
Size: 4226
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 715 2245 715
Size: 1530 2696
FspI TGC'GCA
Cuts at: 2007 2007
Size: 4226
HaeII r_GCGC'y
Cuts at: 223 1660 1908 2930 3909 223
Size: 1437 248 1022 979 540
Fragments arranged by size:
1437 1022 979 540 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 516 548 1090 1115 1122 1147 1198 1230 2068
Size: 32 542 25 7 25 51 32 838
Cuts at: 2068 2098 2100 2130 516
Size: 30 2 30 2612
Fragments arranged by size:
2612 838 542 51 32 32 30 30
25 25 7 2
HincII GTy'rAC
Cuts at: 1265 1265
Size: 4226
KasI G'GCGC_C
Cuts at: 1656 1904 1656
Size: 248 3978
KpnI G_GTAC'C
Cuts at: 241 1246 241
Size: 1005 3221
MluI A'CGCG_T
Cuts at: 3659 3659
Size: 4226
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 341 971 2897 3081 341
Size: 630 1926 184 1486
Fragments arranged by size:
1926 1486 630 184
MscI TGG'CCA
Cuts at: 1089 1987 1089
Size: 898 3328
MslI CAynn'nnrTG
Cuts at: 984 2215 2342 984
Size: 1231 127 2868
Fragments arranged by size:
2868 1231 127
MspA1I CmG'CkG
Cuts at: 877 936 1337 1651 2011 3024 3269 877
Size: 59 401 314 360 1013 245 1834
Fragments arranged by size:
1834 1013 401 360 314 245 59
NaeI GCC'GGC
Cuts at: 2407 2407
Size: 4226
NarI GG'CG_CC
Cuts at: 1657 1905 1657
Size: 248 3978
NcoI C'CATG_G
Cuts at: 107 752 107
Size: 645 3581
NdeI CA'TA_TG
Cuts at: 431 431
Size: 4226
NgoMIV G'CCGG_C
Cuts at: 2405 2405
Size: 4226
NspI r_CATG'y
Cuts at: 1053 1279 1779 2686 1053
Size: 226 500 907 2593
Fragments arranged by size:
2593 907 500 226
PciI A'CATG_T
Cuts at: 2682 2682
Size: 4226
PflMI CCAn_nnn'nTGG
Cuts at: 675 753 990 675
Size: 78 237 3911
Fragments arranged by size:
3911 237 78
PmeI GTTT'AAAC
Cuts at: 1225 1225
Size: 4226
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3365 3397 3365
Size: 32 4194
PpuMI rG'GwC_Cy
Cuts at: 410 924 1126 410
Size: 514 202 3510
Fragments arranged by size:
3510 514 202
PspOMI G'GGCC_C
Cuts at: 203 203
Size: 4226
PstI C_TGCA'G
Cuts at: 956 1145 1273 1958 956
Size: 189 128 685 3224
Fragments arranged by size:
3224 685 189 128
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4226
PvuII CAG'CTG
Cuts at: 877 936 1651 2011 877
Size: 59 715 360 3092
Fragments arranged by size:
3092 715 360 59
RsrII CG'GwC_CG
Cuts at: 2421 2421
Size: 4226
SacI G_AGCT'C
Cuts at: 1240 1240
Size: 4226
SalI G'TCGA_C
Cuts at: 1263 1263
Size: 4226
SanDI GG'GwC_CC
Cuts at: 410 410
Size: 4226
SapI GCTCTTCn'nnn_
Cuts at: 2249 2459 2249
Size: 210 4016
SbfI CC_TGCA'GG
Cuts at: 1145 1273 1145
Size: 128 4098
ScaI AGT'ACT
Cuts at: 470 3743 470
Size: 3273 953
SfcI C'TryA_G
Cuts at: 33 396 952 1141 1269 1954 2947 3138 33
Size: 363 556 189 128 685 993 191 1121
Fragments arranged by size:
1121 993 685 556 363 191 189 128
SfiI GGCCn_nnn'nGGCC
Cuts at: 202 202
Size: 4226
SfoI GGC'GCC
Cuts at: 1658 1906 1658
Size: 248 3978
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4226
SmaI CCC'GGG
Cuts at: 1248 1248
Size: 4226
SmlI C'TyrA_G
Cuts at: 599 1381 2296 2788 3050 599
Size: 782 915 492 262 1775
Fragments arranged by size:
1775 915 782 492 262
SpeI A'CTAG_T
Cuts at: 67 810 67
Size: 743 3483
SphI G_CATG'C
Cuts at: 1279 1779 1279
Size: 500 3726
StyI C'CwwG_G
Cuts at: 107 752 1360 1714 107
Size: 645 608 354 2619
Fragments arranged by size:
2619 645 608 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1809 1942 2401 2610 1809
Size: 133 459 209 3425
Fragments arranged by size:
3425 459 209 133
TatI w'GTAC_w
Cuts at: 468 693 845 1008 3741 468
Size: 225 152 163 2733 953
Fragments arranged by size:
2733 953 225 163 152
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 334 366 3743 3775 334
Size: 32 3377 32 785
Fragments arranged by size:
3377 785 32 32
Tth111I GACn'n_nGTC
Cuts at: 2023 2023
Size: 4226
XbaI T'CTAG_A
Cuts at: 381 726 1257 381
Size: 345 531 3350
Fragments arranged by size:
3350 531 345
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1721 1721
Size: 4226
XmaI C'CCGG_G
Cuts at: 1246 1246
Size: 4226
XmnI GAAnn'nnTTC
Cuts at: 554 554
Size: 4226
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NspI
PciI PflMI PmeI PpiI PpuMI PspOMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbsI BbvCI BcgI BfrBI BmgBI BmtI Bpu10I BsiWI
BsmI BsmBI BsrGI BssHII BstZ17I ClaI CspCI DraIII
FseI FspAI HindIII HpaI MfeI NheI NotI NruI
NsiI PacI PasI PfoI PmlI PshAI PsiI PspXI
PsrI SacII SexAI SgrAI SnaBI SrfI SspI StuI
SwaI XhoI ZraI