Restriction map for
pF1KB7651
(Circular) (Six-base) MAPSORT of: pF1KB7651.seq Check: 6363 from: 1 to: 4103
pF1KB7651 4103 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1141 1141
Size: 4103
Acc65I G'GTAC_C
Cuts at: 1119 1119
Size: 4103
AclI AA'CG_TT
Cuts at: 3943 3943
Size: 4103
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1928 2360 3107 3375 1928
Size: 432 747 268 2656
Fragments arranged by size:
2656 747 432 268
AfeI AGC'GCT
Cuts at: 489 763 489
Size: 274 3829
AflIII A'CryG_T
Cuts at: 1455 2559 3536 1455
Size: 1104 977 2022
Fragments arranged by size:
2022 1104 977
AgeI A'CCGG_T
Cuts at: 2491 2491
Size: 4103
AleI CACnn'nnGTG
Cuts at: 1078 1078
Size: 4103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 949 949
Size: 4103
AlwNI CAG_nnn'CTG
Cuts at: 555 978 1032 1408 2975 555
Size: 423 54 376 1567 1683
Fragments arranged by size:
1683 1567 423 376 54
ApaLI G'TGCA_C
Cuts at: 1000 2873 1000
Size: 1873 2230
ApoI r'AATT_y
Cuts at: 1107 3456 3712 1107
Size: 2349 256 1498
Fragments arranged by size:
2349 1498 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4103
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
AvaI C'yCGr_G
Cuts at: 138 1123 138
Size: 985 3118
BamHI G'GATC_C
Cuts at: 1128 1128
Size: 4103
BanI G'GyrC_C
Cuts at: 265 376 472 507 1119 1533 1781 1816 265
Size: 111 96 35 612 414 248 35 2552
Fragments arranged by size:
2552 612 414 248 111 96 35 35
BanII G_rGCy'C
Cuts at: 155 292 1117 2147 155
Size: 137 825 1030 2111
Fragments arranged by size:
2111 1030 825 137
BbeI G_GCGC'C
Cuts at: 269 380 1537 1785 269
Size: 111 1157 248 2587
Fragments arranged by size:
2587 1157 248 111
BciVI GTATCCnnnnn_n'
Cuts at: 2001 2762 4103 2001
Size: 761 1341 2001
Fragments arranged by size:
2001 1341 761
BclI T'GATC_A
Cuts at: 1623 1623
Size: 4103
BglI GCCn_nnn'nGGC
Cuts at: 356 365 808 356
Size: 9 443 3651
Fragments arranged by size:
3651 443 9
BglII A'GATC_T
Cuts at: 743 1618 3655 743
Size: 875 2037 1191
Fragments arranged by size:
2037 1191 875
BlpI GC'TnA_GC
Cuts at: 1215 1215
Size: 4103
Bme1580I G_kGCm'C
Cuts at: 483 581 1004 1728 1821 2877 483
Size: 98 423 724 93 1056 1709
Fragments arranged by size:
1709 1056 724 423 98 93
BmgBI CAC'GTC
Cuts at: 725 1076 725
Size: 351 3752
BmrI ACTGGGnnnn_n'
Cuts at: 1315 1396 1731 3875 1315
Size: 81 335 2144 1543
Fragments arranged by size:
2144 1543 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2470 2470
Size: 4103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 332 356 775 211
Size: 121 24 419 3539
Fragments arranged by size:
3539 419 121 24
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1279 2158 2650 2948 1279
Size: 879 492 298 2434
Fragments arranged by size:
2434 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3807 3807
Size: 4103
BsaAI yAC'GTr
Cuts at: 1456 1456
Size: 4103
BsaBI GATnn'nnATC
Cuts at: 6 1617 3654 6
Size: 1611 2037 455
Fragments arranged by size:
2037 1611 455
BsaHI Gr'CG_yC
Cuts at: 266 377 602 697 1084 1534 1782 2484 3980
Size: 111 225 95 387 450 248 702 1496
Cuts at: 3980 266
Size: 389
Fragments arranged by size:
1496 702 450 389 387 248 225 111
95
BsaWI w'CCGG_w
Cuts at: 692 1292 1813 2491 2765 2912 3323 692
Size: 600 521 678 274 147 411 1472
Fragments arranged by size:
1472 678 600 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 329 329
Size: 4103
BseYI C'CCAG_C
Cuts at: 548 1528 2286 2863 3359 548
Size: 980 758 577 496 1292
Fragments arranged by size:
1292 980 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 299 794 1645 1691 2899 104
Size: 195 495 851 46 1208 1308
Fragments arranged by size:
1308 1208 851 495 195 46
BsiHKAI G_wGCw'C
Cuts at: 116 1004 1117 1895 2085 2877 116
Size: 888 113 778 190 792 1342
Fragments arranged by size:
1342 888 792 778 190 113
BsmBI CGTCTCn'nnnn_
Cuts at: 870 870
Size: 4103
Bsp1286I G_dGCh'C
Cuts at: 116 155 292 483 581 1004 1117 1728 1821
Size: 39 137 191 98 423 113 611 93
Cuts at: 1821 1895 2085 2147 2877 116
Size: 74 190 62 730 1342
Fragments arranged by size:
1342 730 611 423 191 190 137 113
98 93 74 62 39
BspEI T'CCGG_A
Cuts at: 3323 3323
Size: 4103
BspHI T'CATG_A
Cuts at: 3279 3279
Size: 4103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 828 1153 1367 1669 2050 828
Size: 325 214 302 381 2881
Fragments arranged by size:
2881 381 325 302 214
BsrBI CCG'CTC
Cuts at: 50 319 525 2395 2449 3933 4098 50
Size: 269 206 1870 54 1484 165 55
Fragments arranged by size:
1870 1484 269 206 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2015 2015
Size: 4103
BsrFI r'CCGG_y
Cuts at: 400 510 562 860 2101 2282 2491 400
Size: 110 52 298 1241 181 209 2012
Fragments arranged by size:
2012 1241 298 209 181 110 52
BssHII G'CGCG_C
Cuts at: 798 798
Size: 4103
BssSI C'ACGA_G
Cuts at: 2374 2732 2374
Size: 358 3745
BstAPI GCAn_nnn'nTGC
Cuts at: 1032 1480 1032
Size: 448 3655
BstBI TT'CG_AA
Cuts at: 2464 2464
Size: 4103
BstEII G'GTnAC_C
Cuts at: 676 676
Size: 4103
BstYI r'GATC_y
Cuts at: 1 176 743 1128 1595 1618 1953 2199 3211
Size: 175 567 385 467 23 335 246 1012
Cuts at: 3211 3297 3309 3655 1
Size: 86 12 346 449
Fragments arranged by size:
1012 567 467 449 385 346 335 246
175 86 23 12
BtgI C'CryG_G
Cuts at: 107 293 536 1039 107
Size: 186 243 503 3171
Fragments arranged by size:
3171 503 243 186
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2053 2234 2397 2053
Size: 181 163 3759
Fragments arranged by size:
3759 181 163
BtsI GCAGTG_nn'
Cuts at: 1346 1346
Size: 4103
DraI TTT'AAA
Cuts at: 1102 1102
Size: 4103
DrdI GACnn_nn'nnGTC
Cuts at: 1809 2667 1809
Size: 858 3245
EaeI y'GGCC_r
Cuts at: 296 557 791 809 1688 1862 2253 2280 3625
Size: 261 234 18 879 174 391 27 1345
Cuts at: 3625 296
Size: 774
Fragments arranged by size:
1345 879 774 391 261 234 174 27
18
EagI C'GGCC_G
Cuts at: 296 791 1688 296
Size: 495 897 2711
Fragments arranged by size:
2711 897 495
EarI CTCTTCn'nnn_
Cuts at: 955 1021 2126 2336 955
Size: 66 1105 210 2722
Fragments arranged by size:
2722 1105 210 66
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 879 2311 2621 2767 3913 879
Size: 1432 310 146 1146 1069
Fragments arranged by size:
1432 1146 1069 310 146
EcoICRI GAG'CTC
Cuts at: 1115 1115
Size: 4103
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 211 332 356 775 1928 2360 3107 3375 211
Size: 121 24 419 1153 432 747 268 939
Fragments arranged by size:
1153 939 747 432 419 268 121 24
EcoNI CCTnn'n_nnAGG
Cuts at: 811 811
Size: 4103
EcoO109I rG'GnC_Cy
Cuts at: 211 323 340 391 1242 211
Size: 112 17 51 851 3072
Fragments arranged by size:
3072 851 112 51 17
EcoRI G'AATT_C
Cuts at: 1107 1107
Size: 4103
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2122 2122
Size: 4103
FspI TGC'GCA
Cuts at: 836 1884 836
Size: 1048 3055
HaeII r_GCGC'y
Cuts at: 269 380 491 765 820 1537 1785 2807 3786
Size: 111 111 274 55 717 248 1022 979
Cuts at: 3786 269
Size: 586
Fragments arranged by size:
1022 979 717 586 274 248 111 111
55
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1945 1975 1977 2007 1945
Size: 30 2 30 4041
Fragments arranged by size:
4041 30 30 2
HincII GTy'rAC
Cuts at: 606 1142 606
Size: 536 3567
KasI G'GCGC_C
Cuts at: 265 376 1533 1781 265
Size: 111 1157 248 2587
Fragments arranged by size:
2587 1157 248 111
KpnI G_GTAC'C
Cuts at: 1123 1123
Size: 4103
MluI A'CGCG_T
Cuts at: 3536 3536
Size: 4103
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 240 2774 2958 240
Size: 2534 184 1385
Fragments arranged by size:
2534 1385 184
MscI TGG'CCA
Cuts at: 559 811 1864 559
Size: 252 1053 2798
Fragments arranged by size:
2798 1053 252
MslI CAynn'nnrTG
Cuts at: 1078 2092 2219 1078
Size: 1014 127 2962
Fragments arranged by size:
2962 1014 127
MspA1I CmG'CkG
Cuts at: 295 467 978 985 1214 1528 1888 2901 3146
Size: 172 511 7 229 314 360 1013 245
Cuts at: 3146 295
Size: 1252
Fragments arranged by size:
1252 1013 511 360 314 245 229 172
7
NaeI GCC'GGC
Cuts at: 512 2284 512
Size: 1772 2331
NarI GG'CG_CC
Cuts at: 266 377 1534 1782 266
Size: 111 1157 248 2587
Fragments arranged by size:
2587 1157 248 111
NcoI C'CATG_G
Cuts at: 107 107
Size: 4103
NgoMIV G'CCGG_C
Cuts at: 510 2282 510
Size: 1772 2331
NotI GC'GGCC_GC
Cuts at: 296 296
Size: 4103
NspI r_CATG'y
Cuts at: 1156 1656 2563 1156
Size: 500 907 2696
Fragments arranged by size:
2696 907 500
PciI A'CATG_T
Cuts at: 2559 2559
Size: 4103
PflMI CCAn_nnn'nTGG
Cuts at: 555 555
Size: 4103
PfoI T'CCnGG_A
Cuts at: 227 227
Size: 4103
PmeI GTTT'AAAC
Cuts at: 1102 1102
Size: 4103
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3242 3274 3242
Size: 32 4071
PpuMI rG'GwC_Cy
Cuts at: 211 340 391 211
Size: 129 51 3923
Fragments arranged by size:
3923 129 51
PstI C_TGCA'G
Cuts at: 385 743 938 1028 1037 1150 1835 385
Size: 358 195 90 9 113 685 2653
Fragments arranged by size:
2653 685 358 195 113 90 9
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4103
PvuII CAG'CTG
Cuts at: 1528 1888 1528
Size: 360 3743
RsrII CG'GwC_CG
Cuts at: 2298 2298
Size: 4103
SacI G_AGCT'C
Cuts at: 1117 1117
Size: 4103
SacII CC_GC'GG
Cuts at: 296 296
Size: 4103
SalI G'TCGA_C
Cuts at: 1140 1140
Size: 4103
SanDI GG'GwC_CC
Cuts at: 211 211
Size: 4103
SapI GCTCTTCn'nnn_
Cuts at: 955 1021 2126 2336 955
Size: 66 1105 210 2722
Fragments arranged by size:
2722 1105 210 66
SbfI CC_TGCA'GG
Cuts at: 1150 1150
Size: 4103
ScaI AGT'ACT
Cuts at: 3620 3620
Size: 4103
SexAI A'CCwGG_T
Cuts at: 847 847
Size: 4103
SfcI C'TryA_G
Cuts at: 33 381 739 934 1024 1033 1146 1831 2824
Size: 348 358 195 90 9 113 685 993
Cuts at: 2824 3015 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 358 348 195 191 113
90 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 356 356
Size: 4103
SfoI GGC'GCC
Cuts at: 267 378 1535 1783 267
Size: 111 1157 248 2587
Fragments arranged by size:
2587 1157 248 111
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4103
SmaI CCC'GGG
Cuts at: 140 1125 140
Size: 985 3118
SmlI C'TyrA_G
Cuts at: 1258 2173 2665 2927 1258
Size: 915 492 262 2434
Fragments arranged by size:
2434 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4103
SphI G_CATG'C
Cuts at: 1156 1656 1156
Size: 500 3603
StuI AGG'CCT
Cuts at: 952 952
Size: 4103
StyI C'CwwG_G
Cuts at: 107 246 425 812 1237 1591 107
Size: 139 179 387 425 354 2619
Fragments arranged by size:
2619 425 387 354 179 139
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 844 1686 1819 2278 2487 844
Size: 842 133 459 209 2460
Fragments arranged by size:
2460 842 459 209 133
TatI w'GTAC_w
Cuts at: 3618 3618
Size: 4103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3620 3652 3620
Size: 32 4071
Tth111I GACn'n_nGTC
Cuts at: 1900 1900
Size: 4103
XbaI T'CTAG_A
Cuts at: 1134 1134
Size: 4103
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 114 1598 114
Size: 1484 2619
XmaI C'CCGG_G
Cuts at: 138 1123 138
Size: 985 3118
XmnI GAAnn'nnTTC
Cuts at: 763 763
Size: 4103
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AleI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NotI NspI PciI PflMI PfoI
PmeI PpiI PpuMI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SmlI SpeI SphI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
XmnI
Enzymes that do not cut:
AarI AatII AflII AhdI AloI ApaI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI
BsgI BsiWI BsmI BsrGI BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NruI NsiI PacI PasI PmlI PshAI
PsiI PspOMI PspXI PsrI SgrAI SnaBI SrfI SspI
SwaI XhoI ZraI