Restriction map for
pF1KB7650
(Circular) (Six-base) MAPSORT of: pF1KB7650.seq Check: 6811 from: 1 to: 4289
pF1KB7650 4289 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 1204 1204
Size: 4289
AccI GT'mk_AC
Cuts at: 843 1327 843
Size: 484 3805
Acc65I G'GTAC_C
Cuts at: 1305 1305
Size: 4289
AclI AA'CG_TT
Cuts at: 4129 4129
Size: 4289
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 918 1298 2114 2546 3293 3561 918
Size: 380 816 432 747 268 1646
Fragments arranged by size:
1646 816 747 432 380 268
AfeI AGC'GCT
Cuts at: 166 166
Size: 4289
AflIII A'CryG_T
Cuts at: 1641 2745 3722 1641
Size: 1104 977 2208
Fragments arranged by size:
2208 1104 977
AgeI A'CCGG_T
Cuts at: 2677 2677
Size: 4289
AlwNI CAG_nnn'CTG
Cuts at: 1074 1271 1594 3161 1074
Size: 197 323 1567 2202
Fragments arranged by size:
2202 1567 323 197
ApaLI G'TGCA_C
Cuts at: 323 3059 323
Size: 2736 1553
ApoI r'AATT_y
Cuts at: 526 1293 3642 3898 526
Size: 767 2349 256 917
Fragments arranged by size:
2349 917 767 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4289
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
AvaI C'yCGr_G
Cuts at: 178 1309 178
Size: 1131 3158
BamHI G'GATC_C
Cuts at: 1314 1314
Size: 4289
BanI G'GyrC_C
Cuts at: 1234 1305 1719 1967 2002 1234
Size: 71 414 248 35 3521
Fragments arranged by size:
3521 414 248 71 35
BanII G_rGCy'C
Cuts at: 1303 2333 1303
Size: 1030 3259
BbeI G_GCGC'C
Cuts at: 1723 1971 1723
Size: 248 4041
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 264 298 264
Size: 34 4255
BciVI GTATCCnnnnn_n'
Cuts at: 422 2187 2948 4289 422
Size: 1765 761 1341 422
Fragments arranged by size:
1765 1341 761 422
BclI T'GATC_A
Cuts at: 752 833 1124 1809 752
Size: 81 291 685 3232
Fragments arranged by size:
3232 685 291 81
BglI GCCn_nnn'nGGC
Cuts at: 883 883
Size: 4289
BglII A'GATC_T
Cuts at: 1804 3841 1804
Size: 2037 2252
BlpI GC'TnA_GC
Cuts at: 1401 1401
Size: 4289
Bme1580I G_kGCm'C
Cuts at: 327 1914 2007 3063 327
Size: 1587 93 1056 1553
Fragments arranged by size:
1587 1553 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 1281 1501 1582 1917 4061 1281
Size: 220 81 335 2144 1509
Fragments arranged by size:
2144 1509 335 220 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 1188 2656 1188
Size: 1468 2821
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 451 889 1209 223
Size: 228 438 320 3303
Fragments arranged by size:
3303 438 320 228
Bpu10I CC'TnA_GC
Cuts at: 133 133
Size: 4289
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1465 2344 2836 3134 1465
Size: 879 492 298 2620
Fragments arranged by size:
2620 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3993 3993
Size: 4289
BsaAI yAC'GTr
Cuts at: 1642 1642
Size: 4289
BsaBI GATnn'nnATC
Cuts at: 6 1803 3840 6
Size: 1797 2037 455
Fragments arranged by size:
2037 1797 455
BsaHI Gr'CG_yC
Cuts at: 1720 1968 2670 4166 1720
Size: 248 702 1496 1843
Fragments arranged by size:
1843 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1478 1999 2677 2951 3098 3509 1478
Size: 521 678 274 147 411 2258
Fragments arranged by size:
2258 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 424 454 1207 1237 424
Size: 30 753 30 3476
Fragments arranged by size:
3476 753 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1033 1033
Size: 4289
BseYI C'CCAG_C
Cuts at: 191 1714 2472 3049 3545 191
Size: 1523 758 577 496 935
Fragments arranged by size:
1523 935 758 577 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1182 1182
Size: 4289
BsiEI CG_ry'CG
Cuts at: 104 215 240 1831 1877 3085 104
Size: 111 25 1591 46 1208 1308
Fragments arranged by size:
1591 1308 1208 111 46 25
BsiHKAI G_wGCw'C
Cuts at: 327 1303 2081 2271 3063 327
Size: 976 778 190 792 1553
Fragments arranged by size:
1553 976 792 778 190
Bsp1286I G_dGCh'C
Cuts at: 327 1303 1914 2007 2081 2271 2333 3063 327
Size: 976 611 93 74 190 62 730 1553
Fragments arranged by size:
1553 976 730 611 190 93 74 62
BspEI T'CCGG_A
Cuts at: 3509 3509
Size: 4289
BspHI T'CATG_A
Cuts at: 3465 3465
Size: 4289
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1204 1339 1553 1855 2236 1204
Size: 135 214 302 381 3257
Fragments arranged by size:
3257 381 302 214 135
BsrBI CCG'CTC
Cuts at: 50 2581 2635 4119 4284 50
Size: 2531 54 1484 165 55
Fragments arranged by size:
2531 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1236 2201 1236
Size: 965 3324
BsrFI r'CCGG_y
Cuts at: 210 239 2287 2468 2677 210
Size: 29 2048 181 209 1822
Fragments arranged by size:
2048 1822 209 181 29
BssSI C'ACGA_G
Cuts at: 2560 2918 2560
Size: 358 3931
BstAPI GCAn_nnn'nTGC
Cuts at: 1108 1666 1108
Size: 558 3731
BstBI TT'CG_AA
Cuts at: 2650 2650
Size: 4289
BstEII G'GTnAC_C
Cuts at: 226 774 226
Size: 548 3741
BstYI r'GATC_y
Cuts at: 1 1314 1781 1804 2139 2385 3397 3483 3495
Size: 1313 467 23 335 246 1012 86 12
Cuts at: 3495 3841 1
Size: 346 449
Fragments arranged by size:
1313 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 234 107
Size: 127 4162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2239 2420 2583 2239
Size: 181 163 3945
Fragments arranged by size:
3945 181 163
BtsI GCAGTG_nn'
Cuts at: 1161 1532 1161
Size: 371 3918
DraI TTT'AAA
Cuts at: 1288 1288
Size: 4289
DrdI GACnn_nn'nnGTC
Cuts at: 1995 2853 1995
Size: 858 3431
EaeI y'GGCC_r
Cuts at: 212 237 884 1874 2048 2439 2466 3811 212
Size: 25 647 990 174 391 27 1345 690
Fragments arranged by size:
1345 990 690 647 391 174 27 25
EagI C'GGCC_G
Cuts at: 212 237 1874 212
Size: 25 1637 2627
Fragments arranged by size:
2627 1637 25
EarI CTCTTCn'nnn_
Cuts at: 301 1274 2312 2522 301
Size: 973 1038 210 2068
Fragments arranged by size:
2068 1038 973 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2497 2807 2953 4099 2497
Size: 310 146 1146 2687
Fragments arranged by size:
2687 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1301 1301
Size: 4289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 223 451 889 918 1209 1298 2114 2546 3293
Size: 228 438 29 291 89 816 432 747
Cuts at: 3293 3561 223
Size: 268 951
Fragments arranged by size:
951 816 747 438 432 291 268 228
89 29
EcoNI CCTnn'n_nnAGG
Cuts at: 385 1125 385
Size: 740 3549
EcoO109I rG'GnC_Cy
Cuts at: 127 435 1428 127
Size: 308 993 2988
Fragments arranged by size:
2988 993 308
EcoRI G'AATT_C
Cuts at: 1293 1293
Size: 4289
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 4289
FseI GG_CCGG'CC
Cuts at: 214 214
Size: 4289
FspI TGC'GCA
Cuts at: 2070 2070
Size: 4289
HaeII r_GCGC'y
Cuts at: 168 1723 1971 2993 3972 168
Size: 1555 248 1022 979 485
Fragments arranged by size:
1555 1022 979 485 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 424 456 825 857 950 982 1207 1239 2131
Size: 32 369 32 93 32 225 32 892
Cuts at: 2131 2161 2163 2193 424
Size: 30 2 30 2520
Fragments arranged by size:
2520 892 369 225 93 32 32 32
32 30 30 2
HincII GTy'rAC
Cuts at: 1328 1328
Size: 4289
HindIII A'AGCT_T
Cuts at: 135 135
Size: 4289
KasI G'GCGC_C
Cuts at: 1719 1967 1719
Size: 248 4041
KpnI G_GTAC'C
Cuts at: 1309 1309
Size: 4289
MluI A'CGCG_T
Cuts at: 3722 3722
Size: 4289
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 645 2960 3144 645
Size: 2315 184 1790
Fragments arranged by size:
2315 1790 184
MscI TGG'CCA
Cuts at: 886 2050 886
Size: 1164 3125
MslI CAynn'nnrTG
Cuts at: 760 2278 2405 760
Size: 1518 127 2644
Fragments arranged by size:
2644 1518 127
MspA1I CmG'CkG
Cuts at: 236 264 450 906 1400 1714 2074 3087 3332
Size: 28 186 456 494 314 360 1013 245
Cuts at: 3332 236
Size: 1193
Fragments arranged by size:
1193 1013 494 456 360 314 245 186
28
NaeI GCC'GGC
Cuts at: 212 241 2470 212
Size: 29 2229 2031
Fragments arranged by size:
2229 2031 29
NarI GG'CG_CC
Cuts at: 1720 1968 1720
Size: 248 4041
NcoI C'CATG_G
Cuts at: 107 107
Size: 4289
NgoMIV G'CCGG_C
Cuts at: 210 239 2468 210
Size: 29 2229 2031
Fragments arranged by size:
2229 2031 29
NspI r_CATG'y
Cuts at: 543 1081 1342 1842 2749 543
Size: 538 261 500 907 2083
Fragments arranged by size:
2083 907 538 500 261
PciI A'CATG_T
Cuts at: 2745 2745
Size: 4289
PmeI GTTT'AAAC
Cuts at: 1288 1288
Size: 4289
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3428 3460 3428
Size: 32 4257
PpuMI rG'GwC_Cy
Cuts at: 127 127
Size: 4289
PshAI GACnn'nnGTC
Cuts at: 350 350
Size: 4289
PstI C_TGCA'G
Cuts at: 1336 2021 1336
Size: 685 3604
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4289
PvuII CAG'CTG
Cuts at: 450 1714 2074 450
Size: 1264 360 2665
Fragments arranged by size:
2665 1264 360
RsrII CG'GwC_CG
Cuts at: 2484 2484
Size: 4289
SacI G_AGCT'C
Cuts at: 1303 1303
Size: 4289
SacII CC_GC'GG
Cuts at: 237 237
Size: 4289
SalI G'TCGA_C
Cuts at: 1326 1326
Size: 4289
SanDI GG'GwC_CC
Cuts at: 127 127
Size: 4289
SapI GCTCTTCn'nnn_
Cuts at: 301 2312 2522 301
Size: 2011 210 2068
Fragments arranged by size:
2068 2011 210
SbfI CC_TGCA'GG
Cuts at: 1336 1336
Size: 4289
ScaI AGT'ACT
Cuts at: 3806 3806
Size: 4289
SfcI C'TryA_G
Cuts at: 33 1066 1332 2017 3010 3201 33
Size: 1033 266 685 993 191 1121
Fragments arranged by size:
1121 1033 993 685 266 191
SfoI GGC'GCC
Cuts at: 1721 1969 1721
Size: 248 4041
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4289
SmaI CCC'GGG
Cuts at: 1311 1311
Size: 4289
SmlI C'TyrA_G
Cuts at: 1444 2359 2851 3113 1444
Size: 915 492 262 2620
Fragments arranged by size:
2620 915 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4289
SphI G_CATG'C
Cuts at: 1342 1842 1342
Size: 500 3789
StyI C'CwwG_G
Cuts at: 107 604 1423 1777 107
Size: 497 819 354 2619
Fragments arranged by size:
2619 819 497 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1872 2005 2464 2673 1872
Size: 133 459 209 3488
Fragments arranged by size:
3488 459 209 133
TatI w'GTAC_w
Cuts at: 3804 3804
Size: 4289
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 1191 1223 3806 3838 1191
Size: 32 2583 32 1642
Fragments arranged by size:
2583 1642 32 32
Tth111I GACn'n_nGTC
Cuts at: 2086 2086
Size: 4289
XbaI T'CTAG_A
Cuts at: 1320 1320
Size: 4289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1784 1784
Size: 4289
XmaI C'CCGG_G
Cuts at: 1309 1309
Size: 4289
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I
HincII HindIII KasI KpnI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PciI PmeI
PpiI PpuMI PshAI PstI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfcI SfoI
SgfI SmaI SmlI SpeI SphI StyI TaqII TatI
TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BfrBI BmgBI BmtI BsiWI
BsmI BsmBI BsrGI BssHII BstXI BstZ17I Bsu36I ClaI
CspCI DraIII EcoRV FspAI HpaI MfeI NdeI NheI
NotI NruI NsiI PacI PasI PflMI PfoI PmlI
PsiI PspOMI PspXI PsrI SexAI SfiI SgrAI SnaBI
SrfI SspI StuI SwaI XhoI XmnI ZraI