Restriction map for
pF1KB7646
(Circular) (Six-base) MAPSORT of: pF1KB7646.seq Check: 6119 from: 1 to: 4283
pF1KB7646 4283 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 1321 1321
Size: 4283
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1207 2108 2540 3287 3555 1207
Size: 901 432 747 268 1935
Fragments arranged by size:
1935 901 747 432 268
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AhdI GACnn_n'nnGTC
Cuts at: 1259 1259
Size: 4283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 434 434
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 915 1092 1588 3155 915
Size: 177 496 1567 2043
Fragments arranged by size:
2043 1567 496 177
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 1287 3636 3892 1287
Size: 2349 256 1678
Fragments arranged by size:
2349 1678 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1303 1303
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 1299 1713 1961 1996 1299
Size: 414 248 35 3586
Fragments arranged by size:
3586 414 248 35
BanII G_rGCy'C
Cuts at: 866 1297 2327 866
Size: 431 1030 2822
Fragments arranged by size:
2822 1030 431
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 522 896 1130 1173 1192 522
Size: 374 234 43 19 3613
Fragments arranged by size:
3613 374 234 43 19
BbvCI CC'TCA_GC
Cuts at: 1225 1225
Size: 4283
BciVI GTATCCnnnnn_n'
Cuts at: 631 2181 2942 4283 631
Size: 1550 761 1341 631
Fragments arranged by size:
1550 1341 761 631
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BglII A'GATC_T
Cuts at: 207 1798 3835 207
Size: 1591 2037 655
Fragments arranged by size:
2037 1591 655
BlpI GC'TnA_GC
Cuts at: 1395 1395
Size: 4283
Bme1580I G_kGCm'C
Cuts at: 855 1233 1908 2001 3057 855
Size: 378 675 93 1056 2081
Fragments arranged by size:
2081 1056 675 378 93
BmrI ACTGGGnnnn_n'
Cuts at: 647 746 1495 1576 1911 4055 647
Size: 99 749 81 335 2144 875
Fragments arranged by size:
2144 875 749 335 99 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 811 2650 811
Size: 1839 2444
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 509
Size: 4283
Bpu10I CC'TnA_GC
Cuts at: 1225 1225
Size: 4283
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 115 1173 1459 2338 2830 3128 115
Size: 1058 286 879 492 298 1270
Fragments arranged by size:
1270 1058 879 492 298 286
BsaI GGTCTCn'nnnn_
Cuts at: 155 1267 3987 155
Size: 1112 2720 451
Fragments arranged by size:
2720 1112 451
BsaAI yAC'GTr
Cuts at: 175 1636 175
Size: 1461 2822
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 634 1472 1993 2671 2945 3092 3503 634
Size: 838 521 678 274 147 411 1414
Fragments arranged by size:
1414 838 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 1708 2466 3043 3539 1708
Size: 758 577 496 2452
Fragments arranged by size:
2452 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 866 1297 2075 2265 3057 866
Size: 431 778 190 792 2092
Fragments arranged by size:
2092 792 778 431 190
Bsp1286I G_dGCh'C
Cuts at: 855 866 1233 1297 1908 2001 2075 2265 2327
Size: 11 367 64 611 93 74 190 62
Cuts at: 2327 3057 855
Size: 730 2081
Fragments arranged by size:
2081 730 611 367 190 93 74 64
62 11
BspEI T'CCGG_A
Cuts at: 634 3503 634
Size: 2869 1414
BspHI T'CATG_A
Cuts at: 497 3459 497
Size: 2962 1321
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1333 1547 1849 2230 1333
Size: 214 302 381 3386
Fragments arranged by size:
3386 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2195 2195
Size: 4283
BsrFI r'CCGG_y
Cuts at: 1258 2281 2462 2671 1258
Size: 1023 181 209 2870
Fragments arranged by size:
2870 1023 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 1660 1660
Size: 4283
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstEII G'GTnAC_C
Cuts at: 658 904 927 658
Size: 246 23 4014
Fragments arranged by size:
4014 246 23
BstYI r'GATC_y
Cuts at: 1 207 1088 1308 1775 1798 2133 2379 3391
Size: 206 881 220 467 23 335 246 1012
Cuts at: 3391 3477 3489 3835 1
Size: 86 12 346 449
Fragments arranged by size:
1012 881 467 449 346 335 246 220
206 86 23 12
BtgI C'CryG_G
Cuts at: 107 107
Size: 4283
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 1526 1526
Size: 4283
ClaI AT'CG_AT
Cuts at: 1049 1049
Size: 4283
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 636 671 636
Size: 35 4248
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DraIII CAC_nnn'GTG
Cuts at: 685 685
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 1177 1989 2847 1177
Size: 812 858 2613
Fragments arranged by size:
2613 858 812
EaeI y'GGCC_r
Cuts at: 1868 2042 2433 2460 3805 1868
Size: 174 391 27 1345 2346
Fragments arranged by size:
2346 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 914 2306 2516 914
Size: 1392 210 2681
Fragments arranged by size:
2681 1392 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 548 843 2491 2801 2947 4093 548
Size: 295 1648 310 146 1146 738
Fragments arranged by size:
1648 1146 738 310 295 146
EcoICRI GAG'CTC
Cuts at: 864 1295 864
Size: 431 3852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 509 1207 2108 2540 3287 3555 509
Size: 698 901 432 747 268 1237
Fragments arranged by size:
1237 901 747 698 432 268
EcoNI CCTnn'n_nnAGG
Cuts at: 296 898 1155 296
Size: 602 257 3424
Fragments arranged by size:
3424 602 257
EcoO109I rG'GnC_Cy
Cuts at: 1422 1422
Size: 4283
EcoRI G'AATT_C
Cuts at: 1287 1287
Size: 4283
EcoRV GAT'ATC
Cuts at: 1047 1047
Size: 4283
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2302 2302
Size: 4283
FspI TGC'GCA
Cuts at: 2064 2064
Size: 4283
HaeII r_GCGC'y
Cuts at: 1717 1965 2987 3966 1717
Size: 248 1022 979 2034
Fragments arranged by size:
2034 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1080 1112 1245 1277 2125 2155 2157 2187 1080
Size: 32 133 32 848 30 2 30 3176
Fragments arranged by size:
3176 848 133 32 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 98 2954 3138 98
Size: 2856 184 1243
Fragments arranged by size:
2856 1243 184
MscI TGG'CCA
Cuts at: 2044 2044
Size: 4283
MslI CAynn'nnrTG
Cuts at: 112 416 502 2272 2399 112
Size: 304 86 1770 127 1996
Fragments arranged by size:
1996 1770 304 127 86
MspA1I CmG'CkG
Cuts at: 1224 1394 1708 2068 3081 3326 1224
Size: 170 314 360 1013 245 2181
Fragments arranged by size:
2181 1013 360 314 245 170
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 107 107
Size: 4283
NdeI CA'TA_TG
Cuts at: 231 231
Size: 4283
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NspI r_CATG'y
Cuts at: 415 600 609 1195 1336 1836 2743 415
Size: 185 9 586 141 500 907 1955
Fragments arranged by size:
1955 907 586 500 185 141 9
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PflMI CCAn_nnn'nTGG
Cuts at: 685 915 685
Size: 230 4053
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3422 3454 3422
Size: 32 4251
PstI C_TGCA'G
Cuts at: 1330 2015 1330
Size: 685 3598
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 1224 1708 2068 1224
Size: 484 360 3439
Fragments arranged by size:
3439 484 360
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 866 1297 866
Size: 431 3852
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SexAI A'CCwGG_T
Cuts at: 681 681
Size: 4283
SfcI C'TryA_G
Cuts at: 33 1326 2011 3004 3195 33
Size: 1293 685 993 191 1121
Fragments arranged by size:
1293 1121 993 685 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 130 1152 1438 2353 2845 3107 130
Size: 1022 286 915 492 262 1306
Fragments arranged by size:
1306 1022 915 492 286 262
SpeI A'CTAG_T
Cuts at: 67 382 67
Size: 315 3968
SphI G_CATG'C
Cuts at: 609 1336 1836 609
Size: 727 500 3056
Fragments arranged by size:
3056 727 500
SspI AAT'ATT
Cuts at: 315 315
Size: 4283
StyI C'CwwG_G
Cuts at: 107 1417 1771 107
Size: 1310 354 2619
Fragments arranged by size:
2619 1310 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 479 1866 1999 2458 2667 479
Size: 1387 133 459 209 2095
Fragments arranged by size:
2095 1387 459 209 133
TatI w'GTAC_w
Cuts at: 149 3798 149
Size: 3649 634
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 732 764 3800 3832 732
Size: 32 3036 32 1183
Fragments arranged by size:
3036 1183 32 32
Tth111I GACn'n_nGTC
Cuts at: 2080 2080
Size: 4283
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 326 1778 326
Size: 1452 2831
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BseYI BsiEI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NspI PciI PflMI
PmeI PpiI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SexAI SfcI SfoI SgfI SmaI
SmlI SpeI SphI SspI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI ApaI AscI
AvrII BaeI BcgI BfrBI BglI BmgBI BmtI BsaXI
BseRI BsgI BsiWI BsmI BsmBI BsrGI BssHII BstXI
BstZ17I Bsu36I FseI FspAI HindIII HpaI MfeI NheI
NotI NruI NsiI PacI PasI PfoI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI SacII SanDI SfiI
SgrAI SnaBI SrfI StuI SwaI XhoI XmnI ZraI