Restriction map for
pF1KB7644
(Circular) (Six-base) MAPSORT of: pF1KB7644.seq Check: 5745 from: 1 to: 4283
pF1KB7644 4283 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 449 449
Size: 4283
AccI GT'mk_AC
Cuts at: 332 434 1321 332
Size: 102 887 3294
Fragments arranged by size:
3294 887 102
Acc65I G'GTAC_C
Cuts at: 1299 1299
Size: 4283
AclI AA'CG_TT
Cuts at: 4123 4123
Size: 4283
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 772 2108 2540 3287 3555 256
Size: 516 1336 432 747 268 984
Fragments arranged by size:
1336 984 747 516 432 268
AfeI AGC'GCT
Cuts at: 358 358
Size: 4283
AflIII A'CryG_T
Cuts at: 1635 2739 3716 1635
Size: 1104 977 2202
Fragments arranged by size:
2202 1104 977
AgeI A'CCGG_T
Cuts at: 2671 2671
Size: 4283
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 467 467
Size: 4283
AlwNI CAG_nnn'CTG
Cuts at: 254 266 571 1161 1588 3155 254
Size: 12 305 590 427 1567 1382
Fragments arranged by size:
1567 1382 590 427 305 12
ApaLI G'TGCA_C
Cuts at: 3053 3053
Size: 4283
ApoI r'AATT_y
Cuts at: 493 893 1287 3636 3892 493
Size: 400 394 2349 256 884
Fragments arranged by size:
2349 884 400 394 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4283
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
AvaI C'yCGr_G
Cuts at: 1303 1303
Size: 4283
BamHI G'GATC_C
Cuts at: 1308 1308
Size: 4283
BanI G'GyrC_C
Cuts at: 770 1299 1713 1961 1996 770
Size: 529 414 248 35 3057
Fragments arranged by size:
3057 529 414 248 35
BanII G_rGCy'C
Cuts at: 155 841 1027 1297 2327 155
Size: 686 186 270 1030 2111
Fragments arranged by size:
2111 1030 686 270 186
BbeI G_GCGC'C
Cuts at: 1717 1965 1717
Size: 248 4035
BbsI GAAGACnn'nnnn_
Cuts at: 539 539
Size: 4283
BciVI GTATCCnnnnn_n'
Cuts at: 403 2181 2942 4283 403
Size: 1778 761 1341 403
Fragments arranged by size:
1778 1341 761 403
BclI T'GATC_A
Cuts at: 1803 1803
Size: 4283
BfrBI ATG'CAT
Cuts at: 631 631
Size: 4283
BglI GCCn_nnn'nGGC
Cuts at: 657 657
Size: 4283
BglII A'GATC_T
Cuts at: 1798 3835 1798
Size: 2037 2246
BlpI GC'TnA_GC
Cuts at: 578 874 1395 578
Size: 296 521 3466
Fragments arranged by size:
3466 521 296
Bme1580I G_kGCm'C
Cuts at: 1908 2001 3057 1908
Size: 93 1056 3134
Fragments arranged by size:
3134 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 186 1035 1495 1576 1911 4055 186
Size: 849 460 81 335 2144 414
Fragments arranged by size:
2144 849 460 414 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2650 2650
Size: 4283
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 300 593 634 300
Size: 293 41 3949
Fragments arranged by size:
3949 293 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 1032 1459 2338 2830 3128 256
Size: 776 427 879 492 298 1411
Fragments arranged by size:
1411 879 776 492 427 298
BsaI GGTCTCn'nnnn_
Cuts at: 3987 3987
Size: 4283
BsaAI yAC'GTr
Cuts at: 663 1636 663
Size: 973 3310
BsaBI GATnn'nnATC
Cuts at: 6 1797 3834 6
Size: 1791 2037 455
Fragments arranged by size:
2037 1791 455
BsaHI Gr'CG_yC
Cuts at: 1714 1962 2664 4160 1714
Size: 248 702 1496 1837
Fragments arranged by size:
1837 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1472 1993 2671 2945 3092 3503 1472
Size: 521 678 274 147 411 2252
Fragments arranged by size:
2252 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 625 655 661 691 625
Size: 30 6 30 4217
Fragments arranged by size:
4217 30 30 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 819 819
Size: 4283
BseYI C'CCAG_C
Cuts at: 322 776 1174 1708 2466 3043 3539 322
Size: 454 398 534 758 577 496 1066
Fragments arranged by size:
1066 758 577 534 496 454 398
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 299 427 299
Size: 128 4155
BsiEI CG_ry'CG
Cuts at: 104 1825 1871 3079 104
Size: 1721 46 1208 1308
Fragments arranged by size:
1721 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 841 1297 2075 2265 3057 841
Size: 456 778 190 792 2067
Fragments arranged by size:
2067 792 778 456 190
BsmI GAATG_Cn'
Cuts at: 633 633
Size: 4283
Bsp1286I G_dGCh'C
Cuts at: 155 841 1027 1297 1908 2001 2075 2265 2327
Size: 686 186 270 611 93 74 190 62
Cuts at: 2327 3057 155
Size: 730 1381
Fragments arranged by size:
1381 730 686 611 270 190 186 93
74 62
BspEI T'CCGG_A
Cuts at: 3503 3503
Size: 4283
BspHI T'CATG_A
Cuts at: 3459 3459
Size: 4283
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 1333 1547 1849 2230 449
Size: 884 214 302 381 2502
Fragments arranged by size:
2502 884 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2575 2629 4113 4278 50
Size: 2525 54 1484 165 55
Fragments arranged by size:
2525 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1127 2195 1127
Size: 1068 3215
BsrFI r'CCGG_y
Cuts at: 470 2281 2462 2671 470
Size: 1811 181 209 2082
Fragments arranged by size:
2082 1811 209 181
BssSI C'ACGA_G
Cuts at: 2554 2912 2554
Size: 358 3925
BstAPI GCAn_nnn'nTGC
Cuts at: 371 380 464 1660 371
Size: 9 84 1196 2994
Fragments arranged by size:
2994 1196 84 9
BstBI TT'CG_AA
Cuts at: 2644 2644
Size: 4283
BstXI CCAn_nnnn'nTGG
Cuts at: 199 612 1247 199
Size: 413 635 3235
Fragments arranged by size:
3235 635 413
BstYI r'GATC_y
Cuts at: 1 1308 1775 1798 2133 2379 3391 3477 3489
Size: 1307 467 23 335 246 1012 86 12
Cuts at: 3489 3835 1
Size: 346 449
Fragments arranged by size:
1307 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 131 482 980 1169 131
Size: 351 498 189 3245
Fragments arranged by size:
3245 498 351 189
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2233 2414 2577 2233
Size: 181 163 3939
Fragments arranged by size:
3939 181 163
BtsI GCAGTG_nn'
Cuts at: 444 1215 1526 444
Size: 771 311 3201
Fragments arranged by size:
3201 771 311
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 389 424 389
Size: 35 4248
DraI TTT'AAA
Cuts at: 1282 1282
Size: 4283
DrdI GACnn_nn'nnGTC
Cuts at: 703 1989 2847 703
Size: 1286 858 2139
Fragments arranged by size:
2139 1286 858
EaeI y'GGCC_r
Cuts at: 479 909 1868 2042 2433 2460 3805 479
Size: 430 959 174 391 27 1345 957
Fragments arranged by size:
1345 959 957 430 391 174 27
EagI C'GGCC_G
Cuts at: 1868 1868
Size: 4283
EarI CTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 316 968 2491 2801 2947 4093 316
Size: 652 1523 310 146 1146 506
Fragments arranged by size:
1523 1146 652 506 310 146
EcoICRI GAG'CTC
Cuts at: 839 1295 839
Size: 456 3827
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 256 300 593 634 772 2108 2540 3287 3555
Size: 44 293 41 138 1336 432 747 268
Cuts at: 3555 256
Size: 984
Fragments arranged by size:
1336 984 747 432 293 268 138 44
41
EcoNI CCTnn'n_nnAGG
Cuts at: 505 754 505
Size: 249 4034
EcoO109I rG'GnC_Cy
Cuts at: 240 1180 1422 240
Size: 940 242 3101
Fragments arranged by size:
3101 940 242
EcoRI G'AATT_C
Cuts at: 493 1287 493
Size: 794 3489
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 363 2302 363
Size: 1939 2344
FspI TGC'GCA
Cuts at: 1133 2064 1133
Size: 931 3352
HaeII r_GCGC'y
Cuts at: 360 1717 1965 2987 3966 360
Size: 1357 248 1022 979 677
Fragments arranged by size:
1357 1022 979 677 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 661 693 2125 2155 2157 2187 661
Size: 32 1432 30 2 30 2757
Fragments arranged by size:
2757 1432 32 30 30 2
HincII GTy'rAC
Cuts at: 1322 1322
Size: 4283
KasI G'GCGC_C
Cuts at: 1713 1961 1713
Size: 248 4035
KpnI G_GTAC'C
Cuts at: 1303 1303
Size: 4283
MluI A'CGCG_T
Cuts at: 3716 3716
Size: 4283
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1255 2954 3138 1255
Size: 1699 184 2400
Fragments arranged by size:
2400 1699 184
MscI TGG'CCA
Cuts at: 481 911 2044 481
Size: 430 1133 2720
Fragments arranged by size:
2720 1133 430
MslI CAynn'nnrTG
Cuts at: 2272 2399 2272
Size: 127 4156
MspA1I CmG'CkG
Cuts at: 133 297 477 612 675 776 1394 1708 2068
Size: 164 180 135 63 101 618 314 360
Cuts at: 2068 3081 3326 133
Size: 1013 245 1090
Fragments arranged by size:
1090 1013 618 360 314 245 180 164
135 101 63
NaeI GCC'GGC
Cuts at: 2464 2464
Size: 4283
NarI GG'CG_CC
Cuts at: 1714 1962 1714
Size: 248 4035
NcoI C'CATG_G
Cuts at: 980 1169 980
Size: 189 4094
NgoMIV G'CCGG_C
Cuts at: 2462 2462
Size: 4283
NsiI A_TGCA'T
Cuts at: 633 633
Size: 4283
NspI r_CATG'y
Cuts at: 1138 1336 1836 2743 1138
Size: 198 500 907 2678
Fragments arranged by size:
2678 907 500 198
PciI A'CATG_T
Cuts at: 2739 2739
Size: 4283
PfoI T'CCnGG_A
Cuts at: 155 218 242 155
Size: 63 24 4196
Fragments arranged by size:
4196 63 24
PmeI GTTT'AAAC
Cuts at: 1282 1282
Size: 4283
PmlI CAC'GTG
Cuts at: 663 663
Size: 4283
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 547 579 3422 3454 547
Size: 32 2843 32 1376
Fragments arranged by size:
2843 1376 32 32
PpuMI rG'GwC_Cy
Cuts at: 240 1180 240
Size: 940 3343
PstI C_TGCA'G
Cuts at: 846 1330 2015 846
Size: 484 685 3114
Fragments arranged by size:
3114 685 484
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4283
PvuII CAG'CTG
Cuts at: 297 477 612 675 1708 2068 297
Size: 180 135 63 1033 360 2512
Fragments arranged by size:
2512 1033 360 180 135 63
RsrII CG'GwC_CG
Cuts at: 2478 2478
Size: 4283
SacI G_AGCT'C
Cuts at: 841 1297 841
Size: 456 3827
SacII CC_GC'GG
Cuts at: 134 134
Size: 4283
SalI G'TCGA_C
Cuts at: 1320 1320
Size: 4283
SanDI GG'GwC_CC
Cuts at: 1180 1180
Size: 4283
SapI GCTCTTCn'nnn_
Cuts at: 2306 2516 2306
Size: 210 4073
SbfI CC_TGCA'GG
Cuts at: 1330 1330
Size: 4283
ScaI AGT'ACT
Cuts at: 3800 3800
Size: 4283
SfcI C'TryA_G
Cuts at: 33 842 1326 2011 3004 3195 33
Size: 809 484 685 993 191 1121
Fragments arranged by size:
1121 993 809 685 484 191
SfoI GGC'GCC
Cuts at: 1715 1963 1715
Size: 248 4035
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4283
SmaI CCC'GGG
Cuts at: 1305 1305
Size: 4283
SmlI C'TyrA_G
Cuts at: 271 1011 1438 2353 2845 3107 271
Size: 740 427 915 492 262 1447
Fragments arranged by size:
1447 915 740 492 427 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4283
SphI G_CATG'C
Cuts at: 1138 1336 1836 1138
Size: 198 500 3585
Fragments arranged by size:
3585 500 198
StuI AGG'CCT
Cuts at: 692 692
Size: 4283
StyI C'CwwG_G
Cuts at: 980 1169 1417 1771 980
Size: 189 248 354 3492
Fragments arranged by size:
3492 354 248 189
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1866 1999 2458 2667 1866
Size: 133 459 209 3482
Fragments arranged by size:
3482 459 209 133
TatI w'GTAC_w
Cuts at: 3798 3798
Size: 4283
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3800 3832 3800
Size: 32 4251
Tth111I GACn'n_nGTC
Cuts at: 430 2080 430
Size: 1650 2633
XbaI T'CTAG_A
Cuts at: 1314 1314
Size: 4283
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1778 1778
Size: 4283
XmaI C'CCGG_G
Cuts at: 1303 1303
Size: 4283
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AfeI AflIII AgeI
AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbsI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI
BtgZI BtsI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII KasI KpnI MluI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PciI PfoI PmeI PmlI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XmaI
Enzymes that do not cut:
AatII AflII AhdI AleI AloI ApaI AscI AvrII
BaeI BbvCI BcgI BmgBI BmtI Bpu10I BsiWI BsmBI
BsrGI BssHII BstEII BstZ17I Bsu36I ClaI DraIII EcoRV
FseI FspAI HindIII HpaI MfeI NdeI NheI NotI
NruI PacI PasI PflMI PshAI PsiI PspOMI PspXI
PsrI SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
XhoI XmnI ZraI