Restriction map for
pF1KB7643
(Circular) (Six-base) MAPSORT of: pF1KB7643.seq Check: 6000 from: 1 to: 4010
pF1KB7643 4010 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 468 468
Size: 4010
AccI GT'mk_AC
Cuts at: 1048 1048
Size: 4010
Acc65I G'GTAC_C
Cuts at: 1026 1026
Size: 4010
AclI AA'CG_TT
Cuts at: 3850 3850
Size: 4010
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1835 2267 3014 3282 1835
Size: 432 747 268 2563
Fragments arranged by size:
2563 747 432 268
AflIII A'CryG_T
Cuts at: 326 1362 2466 3443 326
Size: 1036 1104 977 893
Fragments arranged by size:
1104 1036 977 893
AgeI A'CCGG_T
Cuts at: 2398 2398
Size: 4010
AleI CACnn'nnGTG
Cuts at: 536 745 802 536
Size: 209 57 3744
Fragments arranged by size:
3744 209 57
AlwNI CAG_nnn'CTG
Cuts at: 1315 2882 1315
Size: 1567 2443
ApaLI G'TGCA_C
Cuts at: 823 2780 823
Size: 1957 2053
ApoI r'AATT_y
Cuts at: 445 1014 3363 3619 445
Size: 569 2349 256 836
Fragments arranged by size:
2349 836 569 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4010
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
AvaI C'yCGr_G
Cuts at: 317 412 1030 317
Size: 95 618 3297
Fragments arranged by size:
3297 618 95
BamHI G'GATC_C
Cuts at: 1035 1035
Size: 4010
BanI G'GyrC_C
Cuts at: 1026 1440 1688 1723 1026
Size: 414 248 35 3313
Fragments arranged by size:
3313 414 248 35
BanII G_rGCy'C
Cuts at: 528 1024 2054 528
Size: 496 1030 2484
Fragments arranged by size:
2484 1030 496
BbeI G_GCGC'C
Cuts at: 1444 1692 1444
Size: 248 3762
BciVI GTATCCnnnnn_n'
Cuts at: 1908 2669 4010 1908
Size: 761 1341 1908
Fragments arranged by size:
1908 1341 761
BclI T'GATC_A
Cuts at: 1530 1530
Size: 4010
BglI GCCn_nnn'nGGC
Cuts at: 373 373
Size: 4010
BglII A'GATC_T
Cuts at: 1525 3562 1525
Size: 2037 1973
BlpI GC'TnA_GC
Cuts at: 1122 1122
Size: 4010
Bme1580I G_kGCm'C
Cuts at: 425 496 827 1635 1728 2784 425
Size: 71 331 808 93 1056 1651
Fragments arranged by size:
1651 1056 808 331 93 71
BmrI ACTGGGnnnn_n'
Cuts at: 1222 1303 1638 3782 1222
Size: 81 335 2144 1450
Fragments arranged by size:
2144 1450 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2377 2377
Size: 4010
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 148
Size: 4010
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1186 2065 2557 2855 1186
Size: 879 492 298 2341
Fragments arranged by size:
2341 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3714 3714
Size: 4010
BsaAI yAC'GTr
Cuts at: 327 1363 327
Size: 1036 2974
BsaBI GATnn'nnATC
Cuts at: 6 115 1524 3561 6
Size: 109 1409 2037 455
Fragments arranged by size:
2037 1409 455 109
BsaHI Gr'CG_yC
Cuts at: 1441 1689 2391 3887 1441
Size: 248 702 1496 1564
Fragments arranged by size:
1564 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1199 1720 2398 2672 2819 3230 1199
Size: 521 678 274 147 411 1979
Fragments arranged by size:
1979 678 521 411 274 147
BseYI C'CCAG_C
Cuts at: 944 1435 2193 2770 3266 944
Size: 491 758 577 496 1688
Fragments arranged by size:
1688 758 577 496 491
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 741 819 741
Size: 78 3932
BsiEI CG_ry'CG
Cuts at: 104 522 1552 1598 2806 104
Size: 418 1030 46 1208 1308
Fragments arranged by size:
1308 1208 1030 418 46
BsiHKAI G_wGCw'C
Cuts at: 827 1024 1802 1992 2784 827
Size: 197 778 190 792 2053
Fragments arranged by size:
2053 792 778 197 190
BsmI GAATG_Cn'
Cuts at: 554 554
Size: 4010
BsmBI CGTCTCn'nnnn_
Cuts at: 838 838
Size: 4010
Bsp1286I G_dGCh'C
Cuts at: 425 496 528 827 1024 1635 1728 1802 1992
Size: 71 32 299 197 611 93 74 190
Cuts at: 1992 2054 2784 425
Size: 62 730 1651
Fragments arranged by size:
1651 730 611 299 197 190 93 74
71 62 32
BspEI T'CCGG_A
Cuts at: 3230 3230
Size: 4010
BspHI T'CATG_A
Cuts at: 3186 3186
Size: 4010
BspMI ACCTGCnnnn'nnnn_
Cuts at: 368 468 1060 1274 1576 1957 368
Size: 100 592 214 302 381 2421
Fragments arranged by size:
2421 592 381 302 214 100
BsrBI CCG'CTC
Cuts at: 50 147 2302 2356 3840 4005 50
Size: 97 2155 54 1484 165 55
Fragments arranged by size:
2155 1484 165 97 55 54
BsrDI GCAATG_nn'
Cuts at: 693 1922 693
Size: 1229 2781
BsrFI r'CCGG_y
Cuts at: 2008 2189 2398 2008
Size: 181 209 3620
Fragments arranged by size:
3620 209 181
BsrGI T'GTAC_A
Cuts at: 485 638 485
Size: 153 3857
BssSI C'ACGA_G
Cuts at: 2281 2639 2281
Size: 358 3652
BstAPI GCAn_nnn'nTGC
Cuts at: 1387 1387
Size: 4010
BstBI TT'CG_AA
Cuts at: 2371 2371
Size: 4010
BstYI r'GATC_y
Cuts at: 1 1035 1502 1525 1860 2106 3118 3204 3216
Size: 1034 467 23 335 246 1012 86 12
Cuts at: 3216 3562 1
Size: 346 449
Fragments arranged by size:
1034 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 225 1960 2141 2304 225
Size: 1735 181 163 1931
Fragments arranged by size:
1931 1735 181 163
BtsI GCAGTG_nn'
Cuts at: 387 425 531 543 1253 387
Size: 38 106 12 710 3144
Fragments arranged by size:
3144 710 106 38 12
DraI TTT'AAA
Cuts at: 1009 1009
Size: 4010
DrdI GACnn_nn'nnGTC
Cuts at: 1716 2574 1716
Size: 858 3152
EaeI y'GGCC_r
Cuts at: 924 1595 1769 2160 2187 3532 924
Size: 671 174 391 27 1345 1402
Fragments arranged by size:
1402 1345 671 391 174 27
EagI C'GGCC_G
Cuts at: 1595 1595
Size: 4010
EarI CTCTTCn'nnn_
Cuts at: 790 866 2033 2243 790
Size: 76 1167 210 2557
Fragments arranged by size:
2557 1167 210 76
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2218 2528 2674 3820 2218
Size: 310 146 1146 2408
Fragments arranged by size:
2408 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1022 1022
Size: 4010
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 148 1835 2267 3014 3282 148
Size: 1687 432 747 268 876
Fragments arranged by size:
1687 876 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 777 815 1149 777
Size: 38 334 3638
Fragments arranged by size:
3638 334 38
EcoRI G'AATT_C
Cuts at: 445 1014 445
Size: 569 3441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2029 2029
Size: 4010
FspI TGC'GCA
Cuts at: 1791 1791
Size: 4010
HaeII r_GCGC'y
Cuts at: 1444 1692 2714 3693 1444
Size: 248 1022 979 1761
Fragments arranged by size:
1761 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 300 332 1852 1882 1884 1914 300
Size: 32 1520 30 2 30 2396
Fragments arranged by size:
2396 1520 32 30 30 2
HincII GTy'rAC
Cuts at: 588 1049 588
Size: 461 3549
KasI G'GCGC_C
Cuts at: 1440 1688 1440
Size: 248 3762
KpnI G_GTAC'C
Cuts at: 1030 1030
Size: 4010
MfeI C'AATT_G
Cuts at: 859 859
Size: 4010
MluI A'CGCG_T
Cuts at: 3443 3443
Size: 4010
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2681 2865 2681
Size: 184 3826
MscI TGG'CCA
Cuts at: 926 1771 926
Size: 845 3165
MslI CAynn'nnrTG
Cuts at: 536 673 745 802 1999 2126 536
Size: 137 72 57 1197 127 2420
Fragments arranged by size:
2420 1197 137 127 72 57
MspA1I CmG'CkG
Cuts at: 1121 1435 1795 2808 3053 1121
Size: 314 360 1013 245 2078
Fragments arranged by size:
2078 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2191 2191
Size: 4010
NarI GG'CG_CC
Cuts at: 1441 1689 1441
Size: 248 3762
NgoMIV G'CCGG_C
Cuts at: 2189 2189
Size: 4010
NspI r_CATG'y
Cuts at: 1063 1563 2470 1063
Size: 500 907 2603
Fragments arranged by size:
2603 907 500
PasI CC'CwG_GG
Cuts at: 581 581
Size: 4010
PciI A'CATG_T
Cuts at: 2466 2466
Size: 4010
PflMI CCAn_nnn'nTGG
Cuts at: 999 999
Size: 4010
PmeI GTTT'AAAC
Cuts at: 1009 1009
Size: 4010
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3149 3181 3149
Size: 32 3978
PpuMI rG'GwC_Cy
Cuts at: 815 815
Size: 4010
PstI C_TGCA'G
Cuts at: 508 538 1057 1742 508
Size: 30 519 685 2776
Fragments arranged by size:
2776 685 519 30
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4010
PvuII CAG'CTG
Cuts at: 1435 1795 1435
Size: 360 3650
RsrII CG'GwC_CG
Cuts at: 2205 2205
Size: 4010
SacI G_AGCT'C
Cuts at: 1024 1024
Size: 4010
SalI G'TCGA_C
Cuts at: 1047 1047
Size: 4010
SanDI GG'GwC_CC
Cuts at: 815 815
Size: 4010
SapI GCTCTTCn'nnn_
Cuts at: 790 2033 2243 790
Size: 1243 210 2557
Fragments arranged by size:
2557 1243 210
SbfI CC_TGCA'GG
Cuts at: 1057 1057
Size: 4010
ScaI AGT'ACT
Cuts at: 619 3527 619
Size: 2908 1102
SexAI A'CCwGG_T
Cuts at: 259 259
Size: 4010
SfcI C'TryA_G
Cuts at: 33 504 534 1053 1738 2731 2922 33
Size: 471 30 519 685 993 191 1121
Fragments arranged by size:
1121 993 685 519 471 191 30
SfiI GGCCn_nnn'nGGCC
Cuts at: 373 373
Size: 4010
SfoI GGC'GCC
Cuts at: 1442 1690 1442
Size: 248 3762
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4010
SmaI CCC'GGG
Cuts at: 414 1032 414
Size: 618 3392
SmlI C'TyrA_G
Cuts at: 317 1165 2080 2572 2834 317
Size: 848 915 492 262 1493
Fragments arranged by size:
1493 915 848 492 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4010
SphI G_CATG'C
Cuts at: 1063 1563 1063
Size: 500 3510
StyI C'CwwG_G
Cuts at: 972 1144 1498 972
Size: 172 354 3484
Fragments arranged by size:
3484 354 172
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 139 536 753 1593 1726 2185 2394 139
Size: 397 217 840 133 459 209 1755
Fragments arranged by size:
1755 840 459 397 217 209 133
TatI w'GTAC_w
Cuts at: 485 617 638 3525 485
Size: 132 21 2887 970
Fragments arranged by size:
2887 970 132 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3527 3559 3527
Size: 32 3978
Tth111I GACn'n_nGTC
Cuts at: 1807 1807
Size: 4010
XbaI T'CTAG_A
Cuts at: 1041 1041
Size: 4010
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1505 1505
Size: 4010
XhoI C'TCGA_G
Cuts at: 317 317
Size: 4010
XmaI C'CCGG_G
Cuts at: 412 1030 412
Size: 618 3392
XmnI GAAnn'nnTTC
Cuts at: 619 619
Size: 4010
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI
BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I
BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI
BstAPI BstBI BstYI BtgZI BtsI DraI DrdI EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII KasI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PasI PciI PflMI PmeI PpiI PpuMI PstI PvuI
PvuII RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SmaI SmlI SpeI
SphI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AlfI AloI ApaI AscI
AvrII BaeI BbsI BbvCI BcgI BfrBI BmgBI BmtI
Bpu10I BsaXI BseRI BsiWI BssHII BstEII BstXI BstZ17I
Bsu36I BtgI ClaI CspCI DraIII EcoNI EcoRV FseI
FspAI HindIII HpaI NcoI NdeI NheI NotI NruI
NsiI PacI PfoI PmlI PshAI PsiI PspOMI PspXI
PsrI SacII SgrAI SnaBI SrfI SspI StuI SwaI
ZraI