Restriction map for
pF1KB7639
(Circular) (Six-base) MAPSORT of: pF1KB7639.seq Check: 7675 from: 1 to: 4091
pF1KB7639 4091 bp
With 182 enzymes: SgfI *
June 26, 2008 10:49 ..
AccI GT'mk_AC
Cuts at: 124 1129 124
Size: 1005 3086
Acc65I G'GTAC_C
Cuts at: 1107 1107
Size: 4091
AclI AA'CG_TT
Cuts at: 3931 3931
Size: 4091
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 698 1916 2348 3095 3363 698
Size: 1218 432 747 268 1426
Fragments arranged by size:
1426 1218 747 432 268
AflIII A'CryG_T
Cuts at: 797 1443 2547 3524 797
Size: 646 1104 977 1364
Fragments arranged by size:
1364 1104 977 646
AgeI A'CCGG_T
Cuts at: 2479 2479
Size: 4091
AleI CACnn'nnGTG
Cuts at: 817 817
Size: 4091
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 741 741
Size: 4091
AlwNI CAG_nnn'CTG
Cuts at: 780 930 1396 2963 780
Size: 150 466 1567 1908
Fragments arranged by size:
1908 1567 466 150
ApaI G_GGCC'C
Cuts at: 294 633 294
Size: 339 3752
ApaLI G'TGCA_C
Cuts at: 2861 2861
Size: 4091
ApoI r'AATT_y
Cuts at: 528 1095 3444 3700 528
Size: 567 2349 256 919
Fragments arranged by size:
2349 919 567 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4091
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
AvaI C'yCGr_G
Cuts at: 287 1111 287
Size: 824 3267
BamHI G'GATC_C
Cuts at: 1116 1116
Size: 4091
BanI G'GyrC_C
Cuts at: 268 425 460 607 913 1031 1067 1107 1521
Size: 157 35 147 306 118 36 40 414
Cuts at: 1521 1769 1804 268
Size: 248 35 2555
Fragments arranged by size:
2555 414 306 248 157 147 118 40
36 35 35
BanII G_rGCy'C
Cuts at: 239 294 311 633 662 844 875 1105 2135
Size: 55 17 322 29 182 31 230 1030
Cuts at: 2135 239
Size: 2195
Fragments arranged by size:
2195 1030 322 230 182 55 31 29
17
BbeI G_GCGC'C
Cuts at: 272 429 917 1035 1071 1525 1773 272
Size: 157 488 118 36 454 248 2590
Fragments arranged by size:
2590 488 454 248 157 118 36
BciVI GTATCCnnnnn_n'
Cuts at: 1989 2750 4091 1989
Size: 761 1341 1989
Fragments arranged by size:
1989 1341 761
BclI T'GATC_A
Cuts at: 1611 1611
Size: 4091
BglII A'GATC_T
Cuts at: 1606 3643 1606
Size: 2037 2054
BlpI GC'TnA_GC
Cuts at: 282 1203 282
Size: 921 3170
Bme1580I G_kGCm'C
Cuts at: 294 633 1716 1809 2865 294
Size: 339 1083 93 1056 1520
Fragments arranged by size:
1520 1083 1056 339 93
BmgBI CAC'GTC
Cuts at: 798 798
Size: 4091
BmrI ACTGGGnnnn_n'
Cuts at: 593 1303 1384 1719 3863 593
Size: 710 81 335 2144 821
Fragments arranged by size:
2144 821 710 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 573 2458 573
Size: 1885 2206
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 337 343 425 450 253
Size: 84 6 82 25 3894
Fragments arranged by size:
3894 84 82 25 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 813 1267 2146 2638 2936 813
Size: 454 879 492 298 1968
Fragments arranged by size:
1968 879 492 454 298
BsaI GGTCTCn'nnnn_
Cuts at: 3795 3795
Size: 4091
BsaAI yAC'GTr
Cuts at: 1444 1444
Size: 4091
BsaBI GATnn'nnATC
Cuts at: 6 1605 3642 6
Size: 1599 2037 455
Fragments arranged by size:
2037 1599 455
BsaHI Gr'CG_yC
Cuts at: 269 426 887 914 1032 1068 1522 1770 2472
Size: 157 461 27 118 36 454 248 702
Cuts at: 2472 3968 269
Size: 1496 392
Fragments arranged by size:
1496 702 461 454 392 248 157 118
36 27
BsaWI w'CCGG_w
Cuts at: 1280 1801 2479 2753 2900 3311 1280
Size: 521 678 274 147 411 2060
Fragments arranged by size:
2060 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 139 169 139
Size: 30 4061
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 160 1066 160
Size: 906 3185
BseYI C'CCAG_C
Cuts at: 140 363 633 644 1516 2274 2851 3347 140
Size: 223 270 11 872 758 577 496 884
Fragments arranged by size:
884 872 758 577 496 270 223 11
BsiEI CG_ry'CG
Cuts at: 104 1633 1679 2887 104
Size: 1529 46 1208 1308
Fragments arranged by size:
1529 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 239 311 875 1105 1883 2073 2865 239
Size: 72 564 230 778 190 792 1465
Fragments arranged by size:
1465 792 778 564 230 190 72
Bsp1286I G_dGCh'C
Cuts at: 239 294 311 633 662 844 875 1105 1716
Size: 55 17 322 29 182 31 230 611
Cuts at: 1716 1809 1883 2073 2135 2865 239
Size: 93 74 190 62 730 1465
Fragments arranged by size:
1465 730 611 322 230 190 182 93
74 62 55 31 29 17
BspEI T'CCGG_A
Cuts at: 3311 3311
Size: 4091
BspHI T'CATG_A
Cuts at: 3267 3267
Size: 4091
BspMI ACCTGCnnnn'nnnn_
Cuts at: 362 407 1141 1355 1657 2038 362
Size: 45 734 214 302 381 2415
Fragments arranged by size:
2415 734 381 302 214 45
BsrBI CCG'CTC
Cuts at: 50 2383 2437 3921 4086 50
Size: 2333 54 1484 165 55
Fragments arranged by size:
2333 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2003 2003
Size: 4091
BsrFI r'CCGG_y
Cuts at: 428 593 1028 2089 2270 2479 428
Size: 165 435 1061 181 209 2040
Fragments arranged by size:
2040 1061 435 209 181 165
BssHII G'CGCG_C
Cuts at: 415 415
Size: 4091
BssSI C'ACGA_G
Cuts at: 2362 2720 2362
Size: 358 3733
BstAPI GCAn_nnn'nTGC
Cuts at: 375 930 1468 375
Size: 555 538 2998
Fragments arranged by size:
2998 555 538
BstBI TT'CG_AA
Cuts at: 2452 2452
Size: 4091
BstYI r'GATC_y
Cuts at: 1 1116 1583 1606 1941 2187 3199 3285 3297
Size: 1115 467 23 335 246 1012 86 12
Cuts at: 3297 3643 1
Size: 346 449
Fragments arranged by size:
1115 1012 467 449 346 335 246 86
23 12
BtgI C'CryG_G
Cuts at: 107 330 107
Size: 223 3868
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2041 2222 2385 2041
Size: 181 163 3747
Fragments arranged by size:
3747 181 163
BtsI GCAGTG_nn'
Cuts at: 343 824 1334 343
Size: 481 510 3100
Fragments arranged by size:
3100 510 481
DraI TTT'AAA
Cuts at: 1090 1090
Size: 4091
DrdI GACnn_nn'nnGTC
Cuts at: 1797 2655 1797
Size: 858 3233
EaeI y'GGCC_r
Cuts at: 430 1026 1676 1850 2241 2268 3613 430
Size: 596 650 174 391 27 1345 908
Fragments arranged by size:
1345 908 650 596 391 174 27
EagI C'GGCC_G
Cuts at: 1676 1676
Size: 4091
EarI CTCTTCn'nnn_
Cuts at: 477 2114 2324 477
Size: 1637 210 2244
Fragments arranged by size:
2244 1637 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 597 636 2299 2609 2755 3901 597
Size: 39 1663 310 146 1146 787
Fragments arranged by size:
1663 1146 787 310 146 39
EcoICRI GAG'CTC
Cuts at: 237 309 873 1103 237
Size: 72 564 230 3225
Fragments arranged by size:
3225 564 230 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 253 337 343 425 450 698 1916 2348 3095
Size: 84 6 82 25 248 1218 432 747
Cuts at: 3095 3363 253
Size: 268 981
Fragments arranged by size:
1218 981 747 432 268 248 84 82
25 6
EcoNI CCTnn'n_nnAGG
Cuts at: 922 949 961 922
Size: 27 12 4052
Fragments arranged by size:
4052 27 12
EcoO109I rG'GnC_Cy
Cuts at: 629 630 834 927 954 1230 629
Size: 1 204 93 27 276 3490
Fragments arranged by size:
3490 276 204 93 27 1
EcoRI G'AATT_C
Cuts at: 1095 1095
Size: 4091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2110 2110
Size: 4091
FseI GG_CCGG'CC
Cuts at: 597 597
Size: 4091
FspI TGC'GCA
Cuts at: 1872 1872
Size: 4091
HaeII r_GCGC'y
Cuts at: 115 272 330 429 781 917 1035 1071 1525
Size: 157 58 99 352 136 118 36 454
Cuts at: 1525 1773 2795 3774 115
Size: 248 1022 979 432
Fragments arranged by size:
1022 979 454 432 352 248 157 136
118 99 58 36
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 826 858 1933 1963 1965 1995 826
Size: 32 1075 30 2 30 2922
Fragments arranged by size:
2922 1075 32 30 30 2
HincII GTy'rAC
Cuts at: 125 1130 125
Size: 1005 3086
KasI G'GCGC_C
Cuts at: 268 425 913 1031 1067 1521 1769 268
Size: 157 488 118 36 454 248 2590
Fragments arranged by size:
2590 488 454 248 157 118 36
KpnI G_GTAC'C
Cuts at: 1111 1111
Size: 4091
MluI A'CGCG_T
Cuts at: 3524 3524
Size: 4091
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2762 2946 2762
Size: 184 3907
MscI TGG'CCA
Cuts at: 1852 1852
Size: 4091
MslI CAynn'nnrTG
Cuts at: 817 2080 2207 817
Size: 1263 127 2701
Fragments arranged by size:
2701 1263 127
MspA1I CmG'CkG
Cuts at: 299 332 579 792 1202 1516 1876 2889 3134
Size: 33 247 213 410 314 360 1013 245
Cuts at: 3134 299
Size: 1256
Fragments arranged by size:
1256 1013 410 360 314 247 245 213
33
NaeI GCC'GGC
Cuts at: 430 595 1030 2272 430
Size: 165 435 1242 2249
Fragments arranged by size:
2249 1242 435 165
NarI GG'CG_CC
Cuts at: 269 426 914 1032 1068 1522 1770 269
Size: 157 488 118 36 454 248 2590
Fragments arranged by size:
2590 488 454 248 157 118 36
NcoI C'CATG_G
Cuts at: 107 107
Size: 4091
NgoMIV G'CCGG_C
Cuts at: 428 593 1028 2270 428
Size: 165 435 1242 2249
Fragments arranged by size:
2249 1242 435 165
NspI r_CATG'y
Cuts at: 1144 1644 2551 1144
Size: 500 907 2684
Fragments arranged by size:
2684 907 500
PciI A'CATG_T
Cuts at: 2547 2547
Size: 4091
PflMI CCAn_nnn'nTGG
Cuts at: 780 780
Size: 4091
PmeI GTTT'AAAC
Cuts at: 1090 1090
Size: 4091
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3230 3262 3230
Size: 32 4059
PpuMI rG'GwC_Cy
Cuts at: 834 834
Size: 4091
PspOMI G'GGCC_C
Cuts at: 290 629 290
Size: 339 3752
PstI C_TGCA'G
Cuts at: 926 1138 1823 926
Size: 212 685 3194
Fragments arranged by size:
3194 685 212
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4091
PvuII CAG'CTG
Cuts at: 792 1516 1876 792
Size: 724 360 3007
Fragments arranged by size:
3007 724 360
RsrII CG'GwC_CG
Cuts at: 2286 2286
Size: 4091
SacI G_AGCT'C
Cuts at: 239 311 875 1105 239
Size: 72 564 230 3225
Fragments arranged by size:
3225 564 230 72
SacII CC_GC'GG
Cuts at: 333 333
Size: 4091
SalI G'TCGA_C
Cuts at: 123 1128 123
Size: 1005 3086
SapI GCTCTTCn'nnn_
Cuts at: 477 2114 2324 477
Size: 1637 210 2244
Fragments arranged by size:
2244 1637 210
SbfI CC_TGCA'GG
Cuts at: 1138 1138
Size: 4091
ScaI AGT'ACT
Cuts at: 724 3608 724
Size: 2884 1207
SfcI C'TryA_G
Cuts at: 33 450 922 1134 1819 2812 3003 33
Size: 417 472 212 685 993 191 1121
Fragments arranged by size:
1121 993 685 472 417 212 191
SfoI GGC'GCC
Cuts at: 270 427 915 1033 1069 1523 1771 270
Size: 157 488 118 36 454 248 2590
Fragments arranged by size:
2590 488 454 248 157 118 36
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4091
SmaI CCC'GGG
Cuts at: 289 1113 289
Size: 824 3267
SmlI C'TyrA_G
Cuts at: 828 1246 2161 2653 2915 828
Size: 418 915 492 262 2004
Fragments arranged by size:
2004 915 492 418 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4091
SphI G_CATG'C
Cuts at: 1144 1644 1144
Size: 500 3591
SrfI GCCC'GGGC
Cuts at: 289 289
Size: 4091
StuI AGG'CCT
Cuts at: 1081 1081
Size: 4091
StyI C'CwwG_G
Cuts at: 107 743 1225 1579 107
Size: 636 482 354 2619
Fragments arranged by size:
2619 636 482 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 235 1674 1807 2266 2475 235
Size: 1439 133 459 209 1851
Fragments arranged by size:
1851 1439 459 209 133
TatI w'GTAC_w
Cuts at: 722 3606 722
Size: 2884 1207
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 139 171 3608 3640 139
Size: 32 3437 32 590
Fragments arranged by size:
3437 590 32 32
Tth111I GACn'n_nGTC
Cuts at: 1888 1888
Size: 4091
XbaI T'CTAG_A
Cuts at: 1122 1122
Size: 4091
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1586 1586
Size: 4091
XmaI C'CCGG_G
Cuts at: 287 1111 287
Size: 824 3267
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AleI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BciVI BclI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssHII BssSI
BstAPI BstBI BstYI BtgI BtgZI BtsI DraI DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI FseI FspI HaeII Hin4I HincII KasI
KpnI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NspI PciI PflMI PmeI PpiI PpuMI
PspOMI PstI PvuI PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SrfI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AhdI AloI AscI AvrII
BaeI BbsI BbvCI BcgI BfrBI BglI BmtI Bpu10I
BsgI BsiWI BsmI BsmBI BsrGI BstEII BstXI BstZ17I
Bsu36I ClaI CspCI DraIII EcoRV FspAI HindIII HpaI
MfeI NdeI NheI NotI NruI NsiI PacI PasI
PfoI PmlI PshAI PsiI PspXI PsrI SanDI SexAI
SfiI SgrAI SnaBI SspI SwaI XhoI XmnI ZraI