Restriction map for
pF1KB7638
(Circular) (Six-base) MAPSORT of: pF1KB7638.seq Check: 4900 from: 1 to: 4256
pF1KB7638 4256 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 780 1294 780
Size: 514 3742
Acc65I G'GTAC_C
Cuts at: 1272 1272
Size: 4256
AclI AA'CG_TT
Cuts at: 4096 4096
Size: 4256
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 581 828 2081 2513 3260 3528 581
Size: 247 1253 432 747 268 1309
Fragments arranged by size:
1309 1253 747 432 268 247
AflIII A'CryG_T
Cuts at: 1608 2712 3689 1608
Size: 1104 977 2175
Fragments arranged by size:
2175 1104 977
AgeI A'CCGG_T
Cuts at: 2644 2644
Size: 4256
AhdI GACnn_n'nnGTC
Cuts at: 244 244
Size: 4256
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 437 437
Size: 4256
AlwNI CAG_nnn'CTG
Cuts at: 989 1224 1561 3128 989
Size: 235 337 1567 2117
Fragments arranged by size:
2117 1567 337 235
ApaI G_GGCC'C
Cuts at: 255 436 950 1072 255
Size: 181 514 122 3439
Fragments arranged by size:
3439 514 181 122
ApaLI G'TGCA_C
Cuts at: 3026 3026
Size: 4256
ApoI r'AATT_y
Cuts at: 527 1260 3609 3865 527
Size: 733 2349 256 918
Fragments arranged by size:
2349 918 733 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4256
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
AvaI C'yCGr_G
Cuts at: 367 725 857 1276 367
Size: 358 132 419 3347
Fragments arranged by size:
3347 419 358 132
BamHI G'GATC_C
Cuts at: 772 1281 772
Size: 509 3747
BanI G'GyrC_C
Cuts at: 1130 1272 1686 1934 1969 1130
Size: 142 414 248 35 3417
Fragments arranged by size:
3417 414 248 142 35
BanII G_rGCy'C
Cuts at: 255 436 802 858 950 1072 1095 1270 2300
Size: 181 366 56 92 122 23 175 1030
Cuts at: 2300 255
Size: 2211
Fragments arranged by size:
2211 1030 366 181 175 122 92 56
23
BbeI G_GCGC'C
Cuts at: 1690 1938 1690
Size: 248 4008
BbvCI CC'TCA_GC
Cuts at: 794 794
Size: 4256
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 274 308 325 359 274
Size: 34 17 34 4171
Fragments arranged by size:
4171 34 34 17
BciVI GTATCCnnnnn_n'
Cuts at: 2154 2915 4256 2154
Size: 761 1341 2154
Fragments arranged by size:
2154 1341 761
BclI T'GATC_A
Cuts at: 1776 1776
Size: 4256
BglI GCCn_nnn'nGGC
Cuts at: 898 1060 898
Size: 162 4094
BglII A'GATC_T
Cuts at: 1771 3808 1771
Size: 2037 2219
BlpI GC'TnA_GC
Cuts at: 469 1368 469
Size: 899 3357
Bme1580I G_kGCm'C
Cuts at: 255 330 436 950 1010 1072 1881 1974 3030
Size: 75 106 514 60 62 809 93 1056
Cuts at: 3030 255
Size: 1481
Fragments arranged by size:
1481 1056 809 514 106 93 75 62
60
BmrI ACTGGGnnnn_n'
Cuts at: 1468 1549 1884 4028 1468
Size: 81 335 2144 1696
Fragments arranged by size:
2144 1696 335 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 717 2623 717
Size: 1906 2350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 598 646 786 250
Size: 348 48 140 3720
Fragments arranged by size:
3720 348 140 48
Bpu10I CC'TnA_GC
Cuts at: 794 794
Size: 4256
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1128 1231 1432 2311 2803 3101 1128
Size: 103 201 879 492 298 2283
Fragments arranged by size:
2283 879 492 298 201 103
BsaI GGTCTCn'nnnn_
Cuts at: 508 1165 3960 508
Size: 657 2795 804
Fragments arranged by size:
2795 804 657
BsaAI yAC'GTr
Cuts at: 790 1609 790
Size: 819 3437
BsaBI GATnn'nnATC
Cuts at: 6 1770 3807 6
Size: 1764 2037 455
Fragments arranged by size:
2037 1764 455
BsaHI Gr'CG_yC
Cuts at: 1687 1935 2637 4133 1687
Size: 248 702 1496 1810
Fragments arranged by size:
1810 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1445 1966 2644 2918 3065 3476 1445
Size: 521 678 274 147 411 2225
Fragments arranged by size:
2225 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 596 619 626 649 596
Size: 23 7 23 4203
Fragments arranged by size:
4203 23 23 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 253 580 841 844 983 1113 253
Size: 327 261 3 139 130 3396
Fragments arranged by size:
3396 327 261 139 130 3
BseYI C'CCAG_C
Cuts at: 295 536 1182 1681 2439 3016 3512 295
Size: 241 646 499 758 577 496 1039
Fragments arranged by size:
1039 758 646 577 499 496 241
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 298 298
Size: 4256
BsiEI CG_ry'CG
Cuts at: 104 1798 1844 3052 104
Size: 1694 46 1208 1308
Fragments arranged by size:
1694 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 962 1270 2048 2238 3030 962
Size: 308 778 190 792 2188
Fragments arranged by size:
2188 792 778 308 190
Bsp1286I G_dGCh'C
Cuts at: 255 330 436 802 858 950 962 1010 1072
Size: 75 106 366 56 92 12 48 62
Cuts at: 1072 1095 1270 1881 1974 2048 2238 2300 3030
Size: 23 175 611 93 74 190 62 730
Cuts at: 3030 255
Size: 1481
Fragments arranged by size:
1481 730 611 366 190 175 106 93
92 75 74 62 62 56 48 23
12
BspEI T'CCGG_A
Cuts at: 3476 3476
Size: 4256
BspHI T'CATG_A
Cuts at: 3432 3432
Size: 4256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1110 1306 1520 1822 2203 1110
Size: 196 214 302 381 3163
Fragments arranged by size:
3163 381 302 214 196
BsrBI CCG'CTC
Cuts at: 50 594 973 2548 2602 4086 4251 50
Size: 544 379 1575 54 1484 165 55
Fragments arranged by size:
1575 1484 544 379 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2168 2168
Size: 4256
BsrFI r'CCGG_y
Cuts at: 161 2254 2435 2644 161
Size: 2093 181 209 1773
Fragments arranged by size:
2093 1773 209 181
BssSI C'ACGA_G
Cuts at: 2527 2885 2527
Size: 358 3898
BstAPI GCAn_nnn'nTGC
Cuts at: 415 1633 415
Size: 1218 3038
BstBI TT'CG_AA
Cuts at: 2617 2617
Size: 4256
BstYI r'GATC_y
Cuts at: 1 772 1281 1748 1771 2106 2352 3364 3450
Size: 771 509 467 23 335 246 1012 86
Cuts at: 3450 3462 3808 1
Size: 12 346 449
Fragments arranged by size:
1012 771 509 467 449 346 335 246
86 23 12
BtgI C'CryG_G
Cuts at: 176 396 488 1043 176
Size: 220 92 555 3389
Fragments arranged by size:
3389 555 220 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2206 2387 2550 2206
Size: 181 163 3912
Fragments arranged by size:
3912 181 163
BtsI GCAGTG_nn'
Cuts at: 310 409 1208 1499 310
Size: 99 799 291 3067
Fragments arranged by size:
3067 799 291 99
DraI TTT'AAA
Cuts at: 1255 1255
Size: 4256
DrdI GACnn_nn'nnGTC
Cuts at: 1962 2820 1962
Size: 858 3398
EaeI y'GGCC_r
Cuts at: 522 1061 1841 2015 2406 2433 3778 522
Size: 539 780 174 391 27 1345 1000
Fragments arranged by size:
1345 1000 780 539 391 174 27
EagI C'GGCC_G
Cuts at: 1841 1841
Size: 4256
EarI CTCTTCn'nnn_
Cuts at: 557 847 866 950 2279 2489 557
Size: 290 19 84 1329 210 2324
Fragments arranged by size:
2324 1329 290 210 84 19
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 474 2464 2774 2920 4066 474
Size: 1990 310 146 1146 664
Fragments arranged by size:
1990 1146 664 310 146
EcoICRI GAG'CTC
Cuts at: 1268 1268
Size: 4256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 250 581 598 646 786 828 2081 2513 3260
Size: 331 17 48 140 42 1253 432 747
Cuts at: 3260 3528 250
Size: 268 978
Fragments arranged by size:
1253 978 747 432 331 268 140 48
42 17
EcoNI CCTnn'n_nnAGG
Cuts at: 1025 1216 1025
Size: 191 4065
EcoO109I rG'GnC_Cy
Cuts at: 258 432 541 1032 1068 1069 1221 1242 1395
Size: 174 109 491 36 1 152 21 153
Cuts at: 1395 258
Size: 3119
Fragments arranged by size:
3119 491 174 153 152 109 36 21
1
EcoRI G'AATT_C
Cuts at: 1260 1260
Size: 4256
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2275 2275
Size: 4256
FspI TGC'GCA
Cuts at: 2037 2037
Size: 4256
HaeII r_GCGC'y
Cuts at: 1690 1938 2960 3939 1690
Size: 248 1022 979 2007
Fragments arranged by size:
2007 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 230 262 568 596 600 628 2098 2128 2130
Size: 32 306 28 4 28 1470 30 2
Cuts at: 2130 2160 230
Size: 30 2326
Fragments arranged by size:
2326 1470 306 32 30 30 28 28
4 2
HincII GTy'rAC
Cuts at: 781 1295 781
Size: 514 3742
KasI G'GCGC_C
Cuts at: 1686 1934 1686
Size: 248 4008
KpnI G_GTAC'C
Cuts at: 1276 1276
Size: 4256
MluI A'CGCG_T
Cuts at: 3689 3689
Size: 4256
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 2927 3111 2927
Size: 184 4072
MscI TGG'CCA
Cuts at: 524 1063 2017 524
Size: 539 954 2763
Fragments arranged by size:
2763 954 539
MslI CAynn'nnrTG
Cuts at: 2245 2372 2245
Size: 127 4129
MspA1I CmG'CkG
Cuts at: 465 476 837 1127 1367 1681 2041 3054 3299
Size: 11 361 290 240 314 360 1013 245
Cuts at: 3299 465
Size: 1422
Fragments arranged by size:
1422 1013 361 360 314 290 245 240
11
NaeI GCC'GGC
Cuts at: 163 2437 163
Size: 2274 1982
NarI GG'CG_CC
Cuts at: 1687 1935 1687
Size: 248 4008
NcoI C'CATG_G
Cuts at: 176 396 488 1043 176
Size: 220 92 555 3389
Fragments arranged by size:
3389 555 220 92
NgoMIV G'CCGG_C
Cuts at: 161 2435 161
Size: 2274 1982
NspI r_CATG'y
Cuts at: 418 736 1309 1809 2716 418
Size: 318 573 500 907 1958
Fragments arranged by size:
1958 907 573 500 318
PasI CC'CwG_GG
Cuts at: 548 950 1021 548
Size: 402 71 3783
Fragments arranged by size:
3783 402 71
PciI A'CATG_T
Cuts at: 2712 2712
Size: 4256
PflMI CCAn_nnn'nTGG
Cuts at: 531 531
Size: 4256
PmeI GTTT'AAAC
Cuts at: 1255 1255
Size: 4256
PmlI CAC'GTG
Cuts at: 790 790
Size: 4256
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3395 3427 3395
Size: 32 4224
PpuMI rG'GwC_Cy
Cuts at: 541 1032 541
Size: 491 3765
PspOMI G'GGCC_C
Cuts at: 251 432 946 1068 251
Size: 181 514 122 3439
Fragments arranged by size:
3439 514 181 122
PstI C_TGCA'G
Cuts at: 237 1053 1151 1303 1988 237
Size: 816 98 152 685 2505
Fragments arranged by size:
2505 816 685 152 98
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4256
PvuII CAG'CTG
Cuts at: 1681 2041 1681
Size: 360 3896
RsrII CG'GwC_CG
Cuts at: 2451 2451
Size: 4256
SacI G_AGCT'C
Cuts at: 1270 1270
Size: 4256
SalI G'TCGA_C
Cuts at: 779 1293 779
Size: 514 3742
SanDI GG'GwC_CC
Cuts at: 541 1032 541
Size: 491 3765
SapI GCTCTTCn'nnn_
Cuts at: 847 866 950 2279 2489 847
Size: 19 84 1329 210 2614
Fragments arranged by size:
2614 1329 210 84 19
SbfI CC_TGCA'GG
Cuts at: 1303 1303
Size: 4256
ScaI AGT'ACT
Cuts at: 3773 3773
Size: 4256
SfcI C'TryA_G
Cuts at: 33 233 1049 1147 1299 1984 2977 3168 33
Size: 200 816 98 152 685 993 191 1121
Fragments arranged by size:
1121 993 816 685 200 191 152 98
SfoI GGC'GCC
Cuts at: 1688 1936 1688
Size: 248 4008
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4256
SmaI CCC'GGG
Cuts at: 727 859 1278 727
Size: 132 419 3705
Fragments arranged by size:
3705 419 132
SmlI C'TyrA_G
Cuts at: 1107 1246 1411 2326 2818 3080 1107
Size: 139 165 915 492 262 2283
Fragments arranged by size:
2283 915 492 262 165 139
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4256
SphI G_CATG'C
Cuts at: 418 1309 1809 418
Size: 891 500 2865
Fragments arranged by size:
2865 891 500
SrfI GCCC'GGGC
Cuts at: 859 859
Size: 4256
StuI AGG'CCT
Cuts at: 281 281
Size: 4256
StyI C'CwwG_G
Cuts at: 176 396 488 587 863 1043 1390 1744 176
Size: 220 92 99 276 180 347 354 2688
Fragments arranged by size:
2688 354 347 276 220 180 99 92
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 290 935 1839 1972 2431 2640 290
Size: 645 904 133 459 209 1906
Fragments arranged by size:
1906 904 645 459 209 133
TatI w'GTAC_w
Cuts at: 3771 3771
Size: 4256
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 619 651 3773 3805 619
Size: 32 3122 32 1070
Fragments arranged by size:
3122 1070 32 32
Tth111I GACn'n_nGTC
Cuts at: 2053 2053
Size: 4256
XbaI T'CTAG_A
Cuts at: 1287 1287
Size: 4256
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 174 394 1751 174
Size: 220 1357 2679
Fragments arranged by size:
2679 1357 220
XmaI C'CCGG_G
Cuts at: 725 857 1276 725
Size: 132 419 3705
Fragments arranged by size:
3705 419 132
Enzymes that do cut:
AccI Acc65I AclI AcuI AflIII AgeI AhdI AlfI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstBI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I
HincII KasI KpnI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PspOMI PstI PvuI PvuII
RsrII SacI SalI SanDI SapI SbfI ScaI SfcI
SfoI SgfI SmaI SmlI SpeI SphI SrfI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AfeI AflII AleI AloI AscI AvrII
BaeI BbsI BfrBI BmgBI BmtI BsiWI BsmI BsmBI
BsrGI BssHII BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI
NheI NotI NruI NsiI PacI PfoI PshAI PsiI
PspXI PsrI SacII SexAI SfiI SgrAI SnaBI SspI
SwaI XhoI XmnI ZraI