Restriction map for
pF1KB7630
(Circular) (Six-base) MAPSORT of: pF1KB7630.seq Check: 8727 from: 1 to: 4238
pF1KB7630 4238 bp
With 182 enzymes: SgfI *
October 7, 2009 14:24 ..
AccI GT'mk_AC
Cuts at: 1276 1276
Size: 4238
Acc65I G'GTAC_C
Cuts at: 999 1254 999
Size: 255 3983
AclI AA'CG_TT
Cuts at: 4078 4078
Size: 4238
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 217 579 2063 2495 3242 3510 217
Size: 362 1484 432 747 268 945
Fragments arranged by size:
1484 945 747 432 362 268
AflII C'TTAA_G
Cuts at: 427 427
Size: 4238
AflIII A'CryG_T
Cuts at: 865 1590 2694 3671 865
Size: 725 1104 977 1432
Fragments arranged by size:
1432 1104 977 725
AgeI A'CCGG_T
Cuts at: 2626 2626
Size: 4238
AleI CACnn'nnGTG
Cuts at: 523 523
Size: 4238
AlwNI CAG_nnn'CTG
Cuts at: 1166 1543 3110 1166
Size: 377 1567 2294
Fragments arranged by size:
2294 1567 377
ApaI G_GGCC'C
Cuts at: 130 130
Size: 4238
ApaLI G'TGCA_C
Cuts at: 3008 3008
Size: 4238
ApoI r'AATT_y
Cuts at: 1242 3591 3847 1242
Size: 2349 256 1633
Fragments arranged by size:
2349 1633 256
AseI AT'TA_AT
Cuts at: 20 20
Size: 4238
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
AvaI C'yCGr_G
Cuts at: 745 1258 745
Size: 513 3725
BamHI G'GATC_C
Cuts at: 1263 1263
Size: 4238
BanI G'GyrC_C
Cuts at: 999 1254 1668 1916 1951 999
Size: 255 414 248 35 3286
Fragments arranged by size:
3286 414 255 248 35
BanII G_rGCy'C
Cuts at: 130 620 752 764 863 925 970 1028 1252
Size: 490 132 12 99 62 45 58 224
Cuts at: 1252 2282 130
Size: 1030 2086
Fragments arranged by size:
2086 1030 490 224 132 99 62 58
45 12
BbeI G_GCGC'C
Cuts at: 1672 1920 1672
Size: 248 3990
BbvCI CC'TCA_GC
Cuts at: 790 790
Size: 4238
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 90 124 90
Size: 34 4204
BciVI GTATCCnnnnn_n'
Cuts at: 152 2136 2897 4238 152
Size: 1984 761 1341 152
Fragments arranged by size:
1984 1341 761 152
BclI T'GATC_A
Cuts at: 1758 1758
Size: 4238
BfrBI ATG'CAT
Cuts at: 274 645 274
Size: 371 3867
BglII A'GATC_T
Cuts at: 1753 3790 1753
Size: 2037 2201
BlpI GC'TnA_GC
Cuts at: 1350 1350
Size: 4238
Bme1580I G_kGCm'C
Cuts at: 130 1863 1956 3012 130
Size: 1733 93 1056 1356
Fragments arranged by size:
1733 1356 1056 93
BmgBI CAC'GTC
Cuts at: 587 868 1217 587
Size: 281 349 3608
Fragments arranged by size:
3608 349 281
BmrI ACTGGGnnnn_n'
Cuts at: 1086 1159 1450 1531 1866 4010 1086
Size: 73 291 81 335 2144 1314
Fragments arranged by size:
2144 1314 335 291 81 73
BmtI G_CTAG'C
Cuts at: 977 977
Size: 4238
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2605 2605
Size: 4238
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 349 571 975 1102 178
Size: 171 222 404 127 3314
Fragments arranged by size:
3314 404 222 171 127
Bpu10I CC'TnA_GC
Cuts at: 790 790
Size: 4238
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 793 1414 2293 2785 3083 793
Size: 621 879 492 298 1948
Fragments arranged by size:
1948 879 621 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3942 3942
Size: 4238
BsaAI yAC'GTr
Cuts at: 1591 1591
Size: 4238
BsaBI GATnn'nnATC
Cuts at: 6 1752 3789 6
Size: 1746 2037 455
Fragments arranged by size:
2037 1746 455
BsaHI Gr'CG_yC
Cuts at: 1669 1917 2619 4115 1669
Size: 248 702 1496 1792
Fragments arranged by size:
1792 1496 702 248
BsaWI w'CCGG_w
Cuts at: 1427 1948 2626 2900 3047 3458 1427
Size: 521 678 274 147 411 2207
Fragments arranged by size:
2207 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 526 544 556 574 526
Size: 18 12 18 4190
Fragments arranged by size:
4190 18 18 12
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 262 262
Size: 4238
BseYI C'CCAG_C
Cuts at: 497 877 969 1663 2421 2998 3494 497
Size: 380 92 694 758 577 496 1241
Fragments arranged by size:
1241 758 694 577 496 380 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1130 1130
Size: 4238
BsiEI CG_ry'CG
Cuts at: 104 1780 1826 3034 104
Size: 1676 46 1208 1308
Fragments arranged by size:
1676 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 620 764 925 1252 2030 2220 3012 620
Size: 144 161 327 778 190 792 1846
Fragments arranged by size:
1846 792 778 327 190 161 144
BsmI GAATG_Cn'
Cuts at: 274 445 274
Size: 171 4067
Bsp1286I G_dGCh'C
Cuts at: 130 620 752 764 863 925 970 1028 1252
Size: 490 132 12 99 62 45 58 224
Cuts at: 1252 1863 1956 2030 2220 2282 3012 130
Size: 611 93 74 190 62 730 1356
Fragments arranged by size:
1356 730 611 490 224 190 132 99
93 74 62 62 58 45 12
BspEI T'CCGG_A
Cuts at: 3458 3458
Size: 4238
BspHI T'CATG_A
Cuts at: 3414 3414
Size: 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 823 1288 1502 1804 2185 823
Size: 465 214 302 381 2876
Fragments arranged by size:
2876 465 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2530 2584 4068 4233 50
Size: 2480 54 1484 165 55
Fragments arranged by size:
2480 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 2150 2150
Size: 4238
BsrFI r'CCGG_y
Cuts at: 539 2236 2417 2626 539
Size: 1697 181 209 2151
Fragments arranged by size:
2151 1697 209 181
BssSI C'ACGA_G
Cuts at: 2509 2867 2509
Size: 358 3880
BstAPI GCAn_nnn'nTGC
Cuts at: 853 1615 853
Size: 762 3476
BstBI TT'CG_AA
Cuts at: 2599 2599
Size: 4238
BstEII G'GTnAC_C
Cuts at: 492 492
Size: 4238
BstXI CCAn_nnnn'nTGG
Cuts at: 1035 1035
Size: 4238
BstYI r'GATC_y
Cuts at: 1 1263 1730 1753 2088 2334 3346 3432 3444
Size: 1262 467 23 335 246 1012 86 12
Cuts at: 3444 3790 1
Size: 346 449
Fragments arranged by size:
1262 1012 467 449 346 335 246 86
23 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2188 2369 2532 2188
Size: 181 163 3894
Fragments arranged by size:
3894 181 163
BtsI GCAGTG_nn'
Cuts at: 1481 1481
Size: 4238
DraI TTT'AAA
Cuts at: 1237 1237
Size: 4238
DraIII CAC_nnn'GTG
Cuts at: 695 695
Size: 4238
DrdI GACnn_nn'nnGTC
Cuts at: 1944 2802 1944
Size: 858 3380
EaeI y'GGCC_r
Cuts at: 357 1823 1997 2388 2415 3760 357
Size: 1466 174 391 27 1345 835
Fragments arranged by size:
1466 1345 835 391 174 27
EagI C'GGCC_G
Cuts at: 1823 1823
Size: 4238
EarI CTCTTCn'nnn_
Cuts at: 958 2261 2471 958
Size: 1303 210 2725
Fragments arranged by size:
2725 1303 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 134 903 2446 2756 2902 4048 134
Size: 769 1543 310 146 1146 324
Fragments arranged by size:
1543 1146 769 324 310 146
EcoICRI GAG'CTC
Cuts at: 618 762 923 1250 618
Size: 144 161 327 3606
Fragments arranged by size:
3606 327 161 144
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 178 217 349 571 579 975 1102 2063 2495
Size: 39 132 222 8 396 127 961 432
Cuts at: 2495 3242 3510 178
Size: 747 268 906
Fragments arranged by size:
961 906 747 432 396 268 222 132
127 39 8
EcoO109I rG'GnC_Cy
Cuts at: 1377 1377
Size: 4238
EcoRI G'AATT_C
Cuts at: 1242 1242
Size: 4238
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2257 2257
Size: 4238
FspI TGC'GCA
Cuts at: 2019 2019
Size: 4238
HaeII r_GCGC'y
Cuts at: 1157 1672 1920 2942 3921 1157
Size: 515 248 1022 979 1474
Fragments arranged by size:
1474 1022 979 515 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 605 637 654 661 686 693 751 783 2080
Size: 32 17 7 25 7 58 32 1297
Cuts at: 2080 2110 2112 2142 605
Size: 30 2 30 2701
Fragments arranged by size:
2701 1297 58 32 32 30 30 25
17 7 7 2
HincII GTy'rAC
Cuts at: 311 1277 311
Size: 966 3272
KasI G'GCGC_C
Cuts at: 1668 1916 1668
Size: 248 3990
KpnI G_GTAC'C
Cuts at: 1003 1258 1003
Size: 255 3983
MluI A'CGCG_T
Cuts at: 3671 3671
Size: 4238
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 647 753 2909 3093 647
Size: 106 2156 184 1792
Fragments arranged by size:
2156 1792 184 106
MscI TGG'CCA
Cuts at: 1999 1999
Size: 4238
MslI CAynn'nnrTG
Cuts at: 523 1033 2227 2354 523
Size: 510 1194 127 2407
Fragments arranged by size:
2407 1194 510 127
MspA1I CmG'CkG
Cuts at: 1349 1663 2023 3036 3281 1349
Size: 314 360 1013 245 2306
Fragments arranged by size:
2306 1013 360 314 245
NaeI GCC'GGC
Cuts at: 2419 2419
Size: 4238
NarI GG'CG_CC
Cuts at: 1669 1917 1669
Size: 248 3990
NdeI CA'TA_TG
Cuts at: 186 186
Size: 4238
NgoMIV G'CCGG_C
Cuts at: 2417 2417
Size: 4238
NheI G'CTAG_C
Cuts at: 973 973
Size: 4238
NsiI A_TGCA'T
Cuts at: 276 647 276
Size: 371 3867
NspI r_CATG'y
Cuts at: 649 1291 1791 2698 649
Size: 642 500 907 2189
Fragments arranged by size:
2189 907 642 500
PciI A'CATG_T
Cuts at: 2694 2694
Size: 4238
PflMI CCAn_nnn'nTGG
Cuts at: 169 666 169
Size: 497 3741
PmeI GTTT'AAAC
Cuts at: 1237 1237
Size: 4238
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3377 3409 3377
Size: 32 4206
PspOMI G'GGCC_C
Cuts at: 126 126
Size: 4238
PspXI vC'TCGA_Gb
Cuts at: 745 745
Size: 4238
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 538 570 538
Size: 32 4206
PstI C_TGCA'G
Cuts at: 1285 1970 1285
Size: 685 3553
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4238
PvuII CAG'CTG
Cuts at: 1663 2023 1663
Size: 360 3878
RsrII CG'GwC_CG
Cuts at: 2433 2433
Size: 4238
SacI G_AGCT'C
Cuts at: 620 764 925 1252 620
Size: 144 161 327 3606
Fragments arranged by size:
3606 327 161 144
SalI G'TCGA_C
Cuts at: 1275 1275
Size: 4238
SapI GCTCTTCn'nnn_
Cuts at: 958 2261 2471 958
Size: 1303 210 2725
Fragments arranged by size:
2725 1303 210
SbfI CC_TGCA'GG
Cuts at: 1285 1285
Size: 4238
ScaI AGT'ACT
Cuts at: 3755 3755
Size: 4238
SfcI C'TryA_G
Cuts at: 33 204 783 1281 1966 2959 3150 33
Size: 171 579 498 685 993 191 1121
Fragments arranged by size:
1121 993 685 579 498 191 171
SfoI GGC'GCC
Cuts at: 1670 1918 1670
Size: 248 3990
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4238
SmaI CCC'GGG
Cuts at: 1260 1260
Size: 4238
SmlI C'TyrA_G
Cuts at: 427 745 808 1393 2308 2800 3062 427
Size: 318 63 585 915 492 262 1603
Fragments arranged by size:
1603 915 585 492 318 262 63
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4238
SphI G_CATG'C
Cuts at: 649 1291 1791 649
Size: 642 500 3096
Fragments arranged by size:
3096 642 500
SspI AAT'ATT
Cuts at: 212 212
Size: 4238
StuI AGG'CCT
Cuts at: 1225 1225
Size: 4238
StyI C'CwwG_G
Cuts at: 437 1372 1726 437
Size: 935 354 2949
Fragments arranged by size:
2949 935 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 535 1105 1821 1954 2413 2622 535
Size: 570 716 133 459 209 2151
Fragments arranged by size:
2151 716 570 459 209 133
TatI w'GTAC_w
Cuts at: 416 3753 416
Size: 3337 901
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 365 397 3755 3787 365
Size: 32 3358 32 816
Fragments arranged by size:
3358 816 32 32
Tth111I GACn'n_nGTC
Cuts at: 2035 2035
Size: 4238
XbaI T'CTAG_A
Cuts at: 1269 1269
Size: 4238
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 1733 1733
Size: 4238
XhoI C'TCGA_G
Cuts at: 745 745
Size: 4238
XmaI C'CCGG_G
Cuts at: 1258 1258
Size: 4238
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AgeI AleI
AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI
BstEII BstXI BstYI BtgZI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI
FalI FspI HaeII Hin4I HincII KasI KpnI MluI
MmeI MscI MslI MspA1I NaeI NarI NdeI NgoMIV
NheI NsiI NspI PciI PflMI PmeI PpiI PspOMI
PspXI PsrI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AfeI AhdI AlfI AloI AscI AvrII
BaeI BbsI BglI BsiWI BsmBI BsrGI BssHII BstZ17I
Bsu36I BtgI ClaI CspCI EcoNI EcoRV FseI FspAI
HindIII HpaI MfeI NcoI NotI NruI PacI PasI
PfoI PmlI PpuMI PshAI PsiI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SwaI XmnI ZraI