(Circular) (Six-base) MAPSORT of: pF1KB7623.seq Check: 2019 from: 1 to: 4055 pF1KB7623 4055 bp With 182 enzymes: SgfI * June 20, 2008 10:27 .. AccI GT'mk_AC Cuts at: 926 1093 926 Size: 167 3888 Acc65I G'GTAC_C Cuts at: 1071 1071 Size: 4055 AclI AA'CG_TT Cuts at: 3895 3895 Size: 4055 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1880 2312 3059 3327 1880 Size: 432 747 268 2608 Fragments arranged by size: 2608 747 432 268 AflIII A'CryG_T Cuts at: 626 935 1031 1407 2511 3488 626 Size: 309 96 376 1104 977 1193 Fragments arranged by size: 1193 1104 977 376 309 96 AgeI A'CCGG_T Cuts at: 2443 2443 Size: 4055 AlwNI CAG_nnn'CTG Cuts at: 564 1360 2927 564 Size: 796 1567 1692 Fragments arranged by size: 1692 1567 796 ApaI G_GGCC'C Cuts at: 194 308 799 1015 194 Size: 114 491 216 3234 Fragments arranged by size: 3234 491 216 114 ApaLI G'TGCA_C Cuts at: 2825 2825 Size: 4055 ApoI r'AATT_y Cuts at: 1059 3408 3664 1059 Size: 2349 256 1450 Fragments arranged by size: 2349 1450 256 AseI AT'TA_AT Cuts at: 20 20 Size: 4055 AsiSI GCG_AT'CGC Cuts at: 104 104 Size: 4055 AvaI C'yCGr_G Cuts at: 235 307 825 945 1020 1075 235 Size: 72 518 120 75 55 3215 Fragments arranged by size: 3215 518 120 75 72 55 BamHI G'GATC_C Cuts at: 1080 1080 Size: 4055 BanI G'GyrC_C Cuts at: 675 802 1071 1485 1733 1768 675 Size: 127 269 414 248 35 2962 Fragments arranged by size: 2962 414 269 248 127 35 BanII G_rGCy'C Cuts at: 158 194 308 727 799 1015 1069 2099 158 Size: 36 114 419 72 216 54 1030 2114 Fragments arranged by size: 2114 1030 419 216 114 72 54 36 BbeI G_GCGC'C Cuts at: 679 806 1489 1737 679 Size: 127 683 248 2997 Fragments arranged by size: 2997 683 248 127 BciVI GTATCCnnnnn_n' Cuts at: 1953 2714 4055 1953 Size: 761 1341 1953 Fragments arranged by size: 1953 1341 761 BclI T'GATC_A Cuts at: 1575 1575 Size: 4055 BglI GCCn_nnn'nGGC Cuts at: 153 899 1010 153 Size: 746 111 3198 Fragments arranged by size: 3198 746 111 BglII A'GATC_T Cuts at: 1570 3607 1570 Size: 2037 2018 BlpI GC'TnA_GC Cuts at: 1167 1167 Size: 4055 Bme1580I G_kGCm'C Cuts at: 194 308 799 1015 1680 1773 2829 194 Size: 114 491 216 665 93 1056 1420 Fragments arranged by size: 1420 1056 665 491 216 114 93 BmgBI CAC'GTC Cuts at: 627 627 Size: 4055 BmrI ACTGGGnnnn_n' Cuts at: 666 1267 1348 1683 3827 666 Size: 601 81 335 2144 894 Fragments arranged by size: 2144 894 601 335 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 2422 Size: 4055 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 157 157 Size: 4055 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 952 1231 2110 2602 2900 952 Size: 279 879 492 298 2107 Fragments arranged by size: 2107 879 492 298 279 BsaI GGTCTCn'nnnn_ Cuts at: 3759 3759 Size: 4055 BsaAI yAC'GTr Cuts at: 588 938 1408 588 Size: 350 470 3235 Fragments arranged by size: 3235 470 350 BsaBI GATnn'nnATC Cuts at: 6 1569 3606 6 Size: 1563 2037 455 Fragments arranged by size: 2037 1563 455 BsaHI Gr'CG_yC Cuts at: 113 676 803 1486 1734 2436 3932 113 Size: 563 127 683 248 702 1496 236 Fragments arranged by size: 1496 702 683 563 248 236 127 BsaWI w'CCGG_w Cuts at: 1244 1765 2443 2717 2864 3275 1244 Size: 521 678 274 147 411 2024 Fragments arranged by size: 2024 678 521 411 274 147 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 481 759 849 481 Size: 278 90 3687 Fragments arranged by size: 3687 278 90 BseYI C'CCAG_C Cuts at: 354 757 1480 2238 2815 3311 354 Size: 403 723 758 577 496 1098 Fragments arranged by size: 1098 758 723 577 496 403 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 631 631 Size: 4055 BsiEI CG_ry'CG Cuts at: 104 163 1597 1643 2851 104 Size: 59 1434 46 1208 1308 Fragments arranged by size: 1434 1308 1208 59 46 BsiHKAI G_wGCw'C Cuts at: 1069 1847 2037 2829 1069 Size: 778 190 792 2295 Fragments arranged by size: 2295 792 778 190 BsiWI C'GTAC_G Cuts at: 385 896 385 Size: 511 3544 Bsp1286I G_dGCh'C Cuts at: 158 194 308 727 799 1015 1069 1680 1773 Size: 36 114 419 72 216 54 611 93 Cuts at: 1773 1847 2037 2099 2829 158 Size: 74 190 62 730 1384 Fragments arranged by size: 1384 730 611 419 216 190 114 93 74 72 62 54 36 BspEI T'CCGG_A Cuts at: 3275 3275 Size: 4055 BspHI T'CATG_A Cuts at: 3231 3231 Size: 4055 BspMI ACCTGCnnnn'nnnn_ Cuts at: 1105 1319 1621 2002 1105 Size: 214 302 381 3158 Fragments arranged by size: 3158 381 302 214 BsrBI CCG'CTC Cuts at: 50 141 234 687 2347 2401 3885 4050 50 Size: 91 93 453 1660 54 1484 165 55 Fragments arranged by size: 1660 1484 453 165 93 91 55 54 BsrDI GCAATG_nn' Cuts at: 1967 1967 Size: 4055 BsrFI r'CCGG_y Cuts at: 637 853 2053 2234 2443 637 Size: 216 1200 181 209 2249 Fragments arranged by size: 2249 1200 216 209 181 BssSI C'ACGA_G Cuts at: 2326 2684 2326 Size: 358 3697 BstAPI GCAn_nnn'nTGC Cuts at: 335 1432 335 Size: 1097 2958 BstBI TT'CG_AA Cuts at: 2416 2416 Size: 4055 BstXI CCAn_nnnn'nTGG Cuts at: 672 672 Size: 4055 BstYI r'GATC_y Cuts at: 1 1080 1547 1570 1905 2151 3163 3249 3261 Size: 1079 467 23 335 246 1012 86 12 Cuts at: 3261 3607 1 Size: 346 449 Fragments arranged by size: 1079 1012 467 449 346 335 246 86 23 12 BtgI C'CryG_G Cuts at: 107 107 Size: 4055 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 2005 2186 2349 2005 Size: 181 163 3711 Fragments arranged by size: 3711 181 163 BtsI GCAGTG_nn' Cuts at: 1298 1298 Size: 4055 DraI TTT'AAA Cuts at: 1054 1054 Size: 4055 DrdI GACnn_nn'nnGTC Cuts at: 783 1761 2619 783 Size: 978 858 2219 Fragments arranged by size: 2219 978 858 EaeI y'GGCC_r Cuts at: 160 872 1640 1814 2205 2232 3577 160 Size: 712 768 174 391 27 1345 638 Fragments arranged by size: 1345 768 712 638 391 174 27 EagI C'GGCC_G Cuts at: 160 1640 160 Size: 1480 2575 EarI CTCTTCn'nnn_ Cuts at: 2078 2288 2078 Size: 210 3845 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 234 2263 2573 2719 3865 234 Size: 2029 310 146 1146 424 Fragments arranged by size: 2029 1146 424 310 146 EcoICRI GAG'CTC Cuts at: 1067 1067 Size: 4055 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 157 1880 2312 3059 3327 157 Size: 1723 432 747 268 885 Fragments arranged by size: 1723 885 747 432 268 EcoNI CCTnn'n_nnAGG Cuts at: 574 574 Size: 4055 EcoO109I rG'GnC_Cy Cuts at: 190 304 1011 1194 190 Size: 114 707 183 3051 Fragments arranged by size: 3051 707 183 114 EcoRI G'AATT_C Cuts at: 1059 1059 Size: 4055 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2074 2074 Size: 4055 FspI TGC'GCA Cuts at: 1836 1836 Size: 4055 HaeII r_GCGC'y Cuts at: 679 685 806 890 1489 1737 2759 3738 679 Size: 6 121 84 599 248 1022 979 996 Fragments arranged by size: 1022 996 979 599 248 121 84 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1897 1927 1929 1959 1897 Size: 30 2 30 3993 Fragments arranged by size: 3993 30 30 2 HincII GTy'rAC Cuts at: 1094 1094 Size: 4055 KasI G'GCGC_C Cuts at: 675 802 1485 1733 675 Size: 127 683 248 2997 Fragments arranged by size: 2997 683 248 127 KpnI G_GTAC'C Cuts at: 1075 1075 Size: 4055 MluI A'CGCG_T Cuts at: 1031 3488 1031 Size: 2457 1598 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 2726 2910 2726 Size: 184 3871 MscI TGG'CCA Cuts at: 1816 1816 Size: 4055 MslI CAynn'nnrTG Cuts at: 457 778 2044 2171 457 Size: 321 1266 127 2341 Fragments arranged by size: 2341 1266 321 127 MspA1I CmG'CkG Cuts at: 375 564 602 729 1166 1480 1840 2853 3098 Size: 189 38 127 437 314 360 1013 245 Cuts at: 3098 375 Size: 1332 Fragments arranged by size: 1332 1013 437 360 314 245 189 127 38 NaeI GCC'GGC Cuts at: 639 2236 639 Size: 1597 2458 NarI GG'CG_CC Cuts at: 676 803 1486 1734 676 Size: 127 683 248 2997 Fragments arranged by size: 2997 683 248 127 NcoI C'CATG_G Cuts at: 107 107 Size: 4055 NgoMIV G'CCGG_C Cuts at: 637 2234 637 Size: 1597 2458 NspI r_CATG'y Cuts at: 819 1108 1608 2515 819 Size: 289 500 907 2359 Fragments arranged by size: 2359 907 500 289 PciI A'CATG_T Cuts at: 2511 2511 Size: 4055 PflMI CCAn_nnn'nTGG Cuts at: 186 564 186 Size: 378 3677 PmeI GTTT'AAAC Cuts at: 1054 1054 Size: 4055 PmlI CAC'GTG Cuts at: 938 938 Size: 4055 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 412 444 3194 3226 412 Size: 32 2750 32 1241 Fragments arranged by size: 2750 1241 32 32 PspOMI G'GGCC_C Cuts at: 190 304 795 1011 190 Size: 114 491 216 3234 Fragments arranged by size: 3234 491 216 114 PspXI vC'TCGA_Gb Cuts at: 945 945 Size: 4055 PstI C_TGCA'G Cuts at: 1010 1102 1787 1010 Size: 92 685 3278 Fragments arranged by size: 3278 685 92 PvuI CG_AT'CG Cuts at: 104 104 Size: 4055 PvuII CAG'CTG Cuts at: 564 1480 1840 564 Size: 916 360 2779 Fragments arranged by size: 2779 916 360 RsrII CG'GwC_CG Cuts at: 2250 2250 Size: 4055 SacI G_AGCT'C Cuts at: 1069 1069 Size: 4055 SalI G'TCGA_C Cuts at: 1092 1092 Size: 4055 SapI GCTCTTCn'nnn_ Cuts at: 2078 2288 2078 Size: 210 3845 SbfI CC_TGCA'GG Cuts at: 1010 1102 1010 Size: 92 3963 ScaI AGT'ACT Cuts at: 3572 3572 Size: 4055 SfcI C'TryA_G Cuts at: 33 918 1006 1098 1783 2776 2967 33 Size: 885 88 92 685 993 191 1121 Fragments arranged by size: 1121 993 885 685 191 92 88 SfiI GGCCn_nnn'nGGCC Cuts at: 1010 1010 Size: 4055 SfoI GGC'GCC Cuts at: 677 804 1487 1735 677 Size: 127 683 248 2997 Fragments arranged by size: 2997 683 248 127 SgfI GCG_AT'CGC Cuts at: 104 104 Size: 4055 SmaI CCC'GGG Cuts at: 1077 1077 Size: 4055 SmlI C'TyrA_G Cuts at: 235 945 967 1210 2125 2617 2879 235 Size: 710 22 243 915 492 262 1411 Fragments arranged by size: 1411 915 710 492 262 243 22 SpeI A'CTAG_T Cuts at: 67 67 Size: 4055 SphI G_CATG'C Cuts at: 819 1108 1608 819 Size: 289 500 3266 Fragments arranged by size: 3266 500 289 StyI C'CwwG_G Cuts at: 107 299 1189 1543 107 Size: 192 890 354 2619 Fragments arranged by size: 2619 890 354 192 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 771 1638 1771 2230 2439 771 Size: 867 133 459 209 2387 Fragments arranged by size: 2387 867 459 209 133 TatI w'GTAC_w Cuts at: 1040 3570 1040 Size: 2530 1525 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3572 3604 3572 Size: 32 4023 Tth111I GACn'n_nGTC Cuts at: 1852 1852 Size: 4055 XbaI T'CTAG_A Cuts at: 1086 1086 Size: 4055 XcmI CCAnnnn_n'nnnnTGG Cuts at: 1550 1550 Size: 4055 XhoI C'TCGA_G Cuts at: 235 945 235 Size: 710 3345 XmaI C'CCGG_G Cuts at: 1075 1075 Size: 4055 Enzymes that do cut: AccI Acc65I AclI AcuI AflIII AgeI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI BtgI BtgZI BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII KasI KpnI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI PflMI PmeI PmlI PpiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SmaI SmlI SpeI SphI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI Enzymes that do not cut: AarI AatII AfeI AflII AhdI AleI AlfI AloI AscI AvrII BaeI BbsI BbvCI BcgI BfrBI BmtI Bpu10I BsaXI BsmI BsmBI BsrGI BssHII BstEII BstZ17I Bsu36I ClaI CspCI DraIII EcoRV FseI FspAI HindIII HpaI MfeI NdeI NheI NotI NruI NsiI PacI PasI PfoI PpuMI PshAI PsiI PsrI SacII SanDI SexAI SgrAI SnaBI SrfI SspI StuI SwaI XmnI ZraI