Restriction map for pF1KB7623
 (Circular) (Six-base) MAPSORT of: pF1KB7623.seq  Check: 2019  from: 1  to: 4055



pF1KB7623  4055 bp

 With 182 enzymes: SgfI * 

                        June 20, 2008 10:27  ..

AccI GT'mk_AC

Cuts at:    926    1093     926 
   Size:        167    3888
 
Acc65I G'GTAC_C

Cuts at:   1071    1071 
   Size:       4055
 
AclI AA'CG_TT

Cuts at:   3895    3895 
   Size:       4055
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1880    2312    3059    3327    1880 
   Size:        432     747     268    2608

  Fragments arranged by size: 

                2608     747     432     268
 
AflIII A'CryG_T

Cuts at:    626     935    1031    1407    2511    3488     626 
   Size:        309      96     376    1104     977    1193

  Fragments arranged by size: 

                1193    1104     977     376     309      96
 
AgeI A'CCGG_T

Cuts at:   2443    2443 
   Size:       4055
 
AlwNI CAG_nnn'CTG

Cuts at:    564    1360    2927     564 
   Size:        796    1567    1692

  Fragments arranged by size: 

                1692    1567     796
 
ApaI G_GGCC'C

Cuts at:    194     308     799    1015     194 
   Size:        114     491     216    3234

  Fragments arranged by size: 

                3234     491     216     114
 
ApaLI G'TGCA_C

Cuts at:   2825    2825 
   Size:       4055
 
ApoI r'AATT_y

Cuts at:   1059    3408    3664    1059 
   Size:       2349     256    1450

  Fragments arranged by size: 

                2349    1450     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4055
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4055
 
AvaI C'yCGr_G

Cuts at:    235     307     825     945    1020    1075     235 
   Size:         72     518     120      75      55    3215

  Fragments arranged by size: 

                3215     518     120      75      72      55
 
BamHI G'GATC_C

Cuts at:   1080    1080 
   Size:       4055
 
BanI G'GyrC_C

Cuts at:    675     802    1071    1485    1733    1768     675 
   Size:        127     269     414     248      35    2962

  Fragments arranged by size: 

                2962     414     269     248     127      35
 
BanII G_rGCy'C

Cuts at:    158     194     308     727     799    1015    1069    2099     158 
   Size:         36     114     419      72     216      54    1030    2114

  Fragments arranged by size: 

                2114    1030     419     216     114      72      54      36
            
 
BbeI G_GCGC'C

Cuts at:    679     806    1489    1737     679 
   Size:        127     683     248    2997

  Fragments arranged by size: 

                2997     683     248     127
 
BciVI GTATCCnnnnn_n'

Cuts at:   1953    2714    4055    1953 
   Size:        761    1341    1953

  Fragments arranged by size: 

                1953    1341     761
 
BclI T'GATC_A

Cuts at:   1575    1575 
   Size:       4055
 
BglI GCCn_nnn'nGGC

Cuts at:    153     899    1010     153 
   Size:        746     111    3198

  Fragments arranged by size: 

                3198     746     111
 
BglII A'GATC_T

Cuts at:   1570    3607    1570 
   Size:       2037    2018
 
BlpI GC'TnA_GC

Cuts at:   1167    1167 
   Size:       4055
 
Bme1580I G_kGCm'C

Cuts at:    194     308     799    1015    1680    1773    2829     194 
   Size:        114     491     216     665      93    1056    1420

  Fragments arranged by size: 

                1420    1056     665     491     216     114      93
 
BmgBI CAC'GTC

Cuts at:    627     627 
   Size:       4055
 
BmrI ACTGGGnnnn_n'

Cuts at:    666    1267    1348    1683    3827     666 
   Size:        601      81     335    2144     894

  Fragments arranged by size: 

                2144     894     601     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2422    2422 
   Size:       4055
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    157     157 
   Size:       4055
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    952    1231    2110    2602    2900     952 
   Size:        279     879     492     298    2107

  Fragments arranged by size: 

                2107     879     492     298     279
 
BsaI GGTCTCn'nnnn_

Cuts at:   3759    3759 
   Size:       4055
 
BsaAI yAC'GTr

Cuts at:    588     938    1408     588 
   Size:        350     470    3235

  Fragments arranged by size: 

                3235     470     350
 
BsaBI GATnn'nnATC

Cuts at:      6    1569    3606       6 
   Size:       1563    2037     455

  Fragments arranged by size: 

                2037    1563     455
 
BsaHI Gr'CG_yC

Cuts at:    113     676     803    1486    1734    2436    3932     113 
   Size:        563     127     683     248     702    1496     236

  Fragments arranged by size: 

                1496     702     683     563     248     236     127
 
BsaWI w'CCGG_w

Cuts at:   1244    1765    2443    2717    2864    3275    1244 
   Size:        521     678     274     147     411    2024

  Fragments arranged by size: 

                2024     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    481     759     849     481 
   Size:        278      90    3687

  Fragments arranged by size: 

                3687     278      90
 
BseYI C'CCAG_C

Cuts at:    354     757    1480    2238    2815    3311     354 
   Size:        403     723     758     577     496    1098

  Fragments arranged by size: 

                1098     758     723     577     496     403
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    631     631 
   Size:       4055
 
BsiEI CG_ry'CG

Cuts at:    104     163    1597    1643    2851     104 
   Size:         59    1434      46    1208    1308

  Fragments arranged by size: 

                1434    1308    1208      59      46
 
BsiHKAI G_wGCw'C

Cuts at:   1069    1847    2037    2829    1069 
   Size:        778     190     792    2295

  Fragments arranged by size: 

                2295     792     778     190
 
BsiWI C'GTAC_G

Cuts at:    385     896     385 
   Size:        511    3544
 
Bsp1286I G_dGCh'C

Cuts at:    158     194     308     727     799    1015    1069    1680    1773 
   Size:         36     114     419      72     216      54     611      93

Cuts at:   1773    1847    2037    2099    2829     158 
   Size:         74     190      62     730    1384

  Fragments arranged by size: 

                1384     730     611     419     216     190     114      93
                  74      72      62      54      36
 
BspEI T'CCGG_A

Cuts at:   3275    3275 
   Size:       4055
 
BspHI T'CATG_A

Cuts at:   3231    3231 
   Size:       4055
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1105    1319    1621    2002    1105 
   Size:        214     302     381    3158

  Fragments arranged by size: 

                3158     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     141     234     687    2347    2401    3885    4050      50 
   Size:         91      93     453    1660      54    1484     165      55

  Fragments arranged by size: 

                1660    1484     453     165      93      91      55      54
            
 
BsrDI GCAATG_nn'

Cuts at:   1967    1967 
   Size:       4055
 
BsrFI r'CCGG_y

Cuts at:    637     853    2053    2234    2443     637 
   Size:        216    1200     181     209    2249

  Fragments arranged by size: 

                2249    1200     216     209     181
 
BssSI C'ACGA_G

Cuts at:   2326    2684    2326 
   Size:        358    3697
 
BstAPI GCAn_nnn'nTGC

Cuts at:    335    1432     335 
   Size:       1097    2958
 
BstBI TT'CG_AA

Cuts at:   2416    2416 
   Size:       4055
 
BstXI CCAn_nnnn'nTGG

Cuts at:    672     672 
   Size:       4055
 
BstYI r'GATC_y

Cuts at:      1    1080    1547    1570    1905    2151    3163    3249    3261 
   Size:       1079     467      23     335     246    1012      86      12

Cuts at:   3261    3607       1 
   Size:        346     449

  Fragments arranged by size: 

                1079    1012     467     449     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       4055
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2005    2186    2349    2005 
   Size:        181     163    3711

  Fragments arranged by size: 

                3711     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1298    1298 
   Size:       4055
 
DraI TTT'AAA

Cuts at:   1054    1054 
   Size:       4055
 
DrdI GACnn_nn'nnGTC

Cuts at:    783    1761    2619     783 
   Size:        978     858    2219

  Fragments arranged by size: 

                2219     978     858
 
EaeI y'GGCC_r

Cuts at:    160     872    1640    1814    2205    2232    3577     160 
   Size:        712     768     174     391      27    1345     638

  Fragments arranged by size: 

                1345     768     712     638     391     174      27
 
EagI C'GGCC_G

Cuts at:    160    1640     160 
   Size:       1480    2575
 
EarI CTCTTCn'nnn_

Cuts at:   2078    2288    2078 
   Size:        210    3845
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    234    2263    2573    2719    3865     234 
   Size:       2029     310     146    1146     424

  Fragments arranged by size: 

                2029    1146     424     310     146
 
EcoICRI GAG'CTC

Cuts at:   1067    1067 
   Size:       4055
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    157    1880    2312    3059    3327     157 
   Size:       1723     432     747     268     885

  Fragments arranged by size: 

                1723     885     747     432     268
 
EcoNI CCTnn'n_nnAGG

Cuts at:    574     574 
   Size:       4055
 
EcoO109I rG'GnC_Cy

Cuts at:    190     304    1011    1194     190 
   Size:        114     707     183    3051

  Fragments arranged by size: 

                3051     707     183     114
 
EcoRI G'AATT_C

Cuts at:   1059    1059 
   Size:       4055
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2074    2074 
   Size:       4055
 
FspI TGC'GCA

Cuts at:   1836    1836 
   Size:       4055
 
HaeII r_GCGC'y

Cuts at:    679     685     806     890    1489    1737    2759    3738     679 
   Size:          6     121      84     599     248    1022     979     996

  Fragments arranged by size: 

                1022     996     979     599     248     121      84       6
            
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1897    1927    1929    1959    1897 
   Size:         30       2      30    3993

  Fragments arranged by size: 

                3993      30      30       2
 
HincII GTy'rAC

Cuts at:   1094    1094 
   Size:       4055
 
KasI G'GCGC_C

Cuts at:    675     802    1485    1733     675 
   Size:        127     683     248    2997

  Fragments arranged by size: 

                2997     683     248     127
 
KpnI G_GTAC'C

Cuts at:   1075    1075 
   Size:       4055
 
MluI A'CGCG_T

Cuts at:   1031    3488    1031 
   Size:       2457    1598
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2726    2910    2726 
   Size:        184    3871
 
MscI TGG'CCA

Cuts at:   1816    1816 
   Size:       4055
 
MslI CAynn'nnrTG

Cuts at:    457     778    2044    2171     457 
   Size:        321    1266     127    2341

  Fragments arranged by size: 

                2341    1266     321     127
 
MspA1I CmG'CkG

Cuts at:    375     564     602     729    1166    1480    1840    2853    3098 
   Size:        189      38     127     437     314     360    1013     245

Cuts at:   3098     375 
   Size:       1332

  Fragments arranged by size: 

                1332    1013     437     360     314     245     189     127
                  38
 
NaeI GCC'GGC

Cuts at:    639    2236     639 
   Size:       1597    2458
 
NarI GG'CG_CC

Cuts at:    676     803    1486    1734     676 
   Size:        127     683     248    2997

  Fragments arranged by size: 

                2997     683     248     127
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4055
 
NgoMIV G'CCGG_C

Cuts at:    637    2234     637 
   Size:       1597    2458
 
NspI r_CATG'y

Cuts at:    819    1108    1608    2515     819 
   Size:        289     500     907    2359

  Fragments arranged by size: 

                2359     907     500     289
 
PciI A'CATG_T

Cuts at:   2511    2511 
   Size:       4055
 
PflMI CCAn_nnn'nTGG

Cuts at:    186     564     186 
   Size:        378    3677
 
PmeI GTTT'AAAC

Cuts at:   1054    1054 
   Size:       4055
 
PmlI CAC'GTG

Cuts at:    938     938 
   Size:       4055
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    412     444    3194    3226     412 
   Size:         32    2750      32    1241

  Fragments arranged by size: 

                2750    1241      32      32
 
PspOMI G'GGCC_C

Cuts at:    190     304     795    1011     190 
   Size:        114     491     216    3234

  Fragments arranged by size: 

                3234     491     216     114
 
PspXI vC'TCGA_Gb

Cuts at:    945     945 
   Size:       4055
 
PstI C_TGCA'G

Cuts at:   1010    1102    1787    1010 
   Size:         92     685    3278

  Fragments arranged by size: 

                3278     685      92
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4055
 
PvuII CAG'CTG

Cuts at:    564    1480    1840     564 
   Size:        916     360    2779

  Fragments arranged by size: 

                2779     916     360
 
RsrII CG'GwC_CG

Cuts at:   2250    2250 
   Size:       4055
 
SacI G_AGCT'C

Cuts at:   1069    1069 
   Size:       4055
 
SalI G'TCGA_C

Cuts at:   1092    1092 
   Size:       4055
 
SapI GCTCTTCn'nnn_

Cuts at:   2078    2288    2078 
   Size:        210    3845
 
SbfI CC_TGCA'GG

Cuts at:   1010    1102    1010 
   Size:         92    3963
 
ScaI AGT'ACT

Cuts at:   3572    3572 
   Size:       4055
 
SfcI C'TryA_G

Cuts at:     33     918    1006    1098    1783    2776    2967      33 
   Size:        885      88      92     685     993     191    1121

  Fragments arranged by size: 

                1121     993     885     685     191      92      88
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   1010    1010 
   Size:       4055
 
SfoI GGC'GCC

Cuts at:    677     804    1487    1735     677 
   Size:        127     683     248    2997

  Fragments arranged by size: 

                2997     683     248     127
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4055
 
SmaI CCC'GGG

Cuts at:   1077    1077 
   Size:       4055
 
SmlI C'TyrA_G

Cuts at:    235     945     967    1210    2125    2617    2879     235 
   Size:        710      22     243     915     492     262    1411

  Fragments arranged by size: 

                1411     915     710     492     262     243      22
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4055
 
SphI G_CATG'C

Cuts at:    819    1108    1608     819 
   Size:        289     500    3266

  Fragments arranged by size: 

                3266     500     289
 
StyI C'CwwG_G

Cuts at:    107     299    1189    1543     107 
   Size:        192     890     354    2619

  Fragments arranged by size: 

                2619     890     354     192
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    771    1638    1771    2230    2439     771 
   Size:        867     133     459     209    2387

  Fragments arranged by size: 

                2387     867     459     209     133
 
TatI w'GTAC_w

Cuts at:   1040    3570    1040 
   Size:       2530    1525
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3572    3604    3572 
   Size:         32    4023
 
Tth111I GACn'n_nGTC

Cuts at:   1852    1852 
   Size:       4055
 
XbaI T'CTAG_A

Cuts at:   1086    1086 
   Size:       4055
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1550    1550 
   Size:       4055
 
XhoI C'TCGA_G

Cuts at:    235     945     235 
   Size:        710    3345
 
XmaI C'CCGG_G

Cuts at:   1075    1075 
   Size:       4055
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI     ApaI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstXI    BstYI     BtgI    BtgZI     BtsI     DraI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI     NcoI
   NgoMIV     NspI     PciI    PflMI     PmeI     PmlI     PpiI   PspOMI
    PspXI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI     BmtI
   Bpu10I    BsaXI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I
   Bsu36I     ClaI    CspCI   DraIII    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NdeI     NheI     NotI     NruI     NsiI     PacI
     PasI     PfoI    PpuMI    PshAI     PsiI     PsrI    SacII    SanDI
    SexAI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XmnI
     ZraI
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